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Promega amv reverse transcriptase kit
Amv Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+kit/amv+reverse+transcriptase+kit/pmc01924770-94-9-13
Average 90 stars, based on 1 article reviews
amv reverse transcriptase kit - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Synthetic auxotrophy reveals metabolic regulation of plasma cell generation, affinity maturation, and cytokine receptor signaling
Article Snippet: RNA was isolated from sorted B cells or in vitro-generated B lymphoblasts using TRIzol reagent following the manufacturer’s instructions (Life Technologies, Carlsbad, CA).

Reverse Transcription:

Article Title: AKRs confer oligodendrocytes resistance to differentiation-stimulated ferroptosis.
Article Snippet: TripleXtactor reagent (Smobio) was used to isolate total RNA. .. The AMV Reverse Transcriptase kit (Promega) was used to produce cDNA following the manufacturer’s recommendations. .. Quantitative PCR reactions were performed by using a CFX96 (Bio-Rad) thermocycler.

Article Title: Prevalence and Risk Factors of Occult HCV Infection in the Adult Population of Mexico City.
Article Snippet: RNA from plasma and PBMCs was quantified on a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and adjusted to a 20 ng/μL concentration. .. The cDNA was obtained by reverse transcription using the AMV reverse transcriptase kit and random hexamer primers (Promega, Madison, WI, USA), following the manufacturer’s instructions. ..

Article Title: The aryl hydrocarbon receptor controls mesenchymal stromal cell-mediated immunomodulation via ubiquitination of eukaryotic elongation factor-2 kinase
Article Snippet: After 24 h, MSCs were harvested to extract the total mRNA. mRNA was extracted from MSCs using the TRIzol reagent (Gibco). .. According to the manufacturer’s instructions, cDNA was synthesized using an AMV reverse transcriptase Kit (Promega, Madison, WI, USA). .. The cDNA products were amplified using the Kapa SYBR qPCR Kit master mix (2x) from Kapa Biosystems (Sigma-Aldrich). cDNA was amplified using primers specific for iNOS and β-actin (Qiagen, Hilden, Germany).

Article Title: T Cell-Induced Colitis Is Exacerbated by Prolonged Stress: A Comparison in Male and Female Mice
Article Snippet: Mid colons were snap frozen in liquid nitrogen and stored at −80 °C until isolating total RNA using Tri-zol reagent (Invitrogen, Carlsbad, CA, USA) following manufacturer protocols. .. Complimentary DNA was synthesized with the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA). .. All mouse mRNA primers were purchased from Integrated DNA Technologies (Redwood City, CA, USA).

Article Title: The Impact of Varying Alginate Co-activation with Probiotics on the Artemia Bioencapsulation to Enhance Immunity Against Vibrio spp.
Article Snippet: The total RNA was extracted from Artemia's whole body with TRIzol Reagent (Thermo Fisher Scientific) based on the procedure by the manufacturer. .. The total RNA was then synthesized to get cDNA using AMV Reverse Transcriptase Kit (Promega Corp.) at 42°C for 30 min, followed by transcriptase enzyme inactivated at 94°C for 5 min. .. The expression of proPO, LGBP, and lectin was analyzed relatively with an internal control using Sybr Green Master Mix (Kapa SYBER FAST qPCR Master Mix) (Kapa biosystems).

Article Title: AKRs confer oligodendrocytes resistance to differentiation-stimulated ferroptosis
Article Snippet: TripleXtactor reagent (Smobio) was used to isolate total RNA. .. The AMV Reverse Transcriptase kit (Promega) was used to produce cDNA following the manufacturer's recommendations. .. Quantitative PCR reactions were performed by using a CFX96 (Bio-Rad) thermocycler.

Article Title: Down-regulating nuclear factor of activated T cells 1 alleviates cognitive deficits in a mouse model of sepsis-associated encephalopathy, possibly by stimulating hippocampal neurogenesis.
Article Snippet: Sepsis-associated encephalopathy (SAE) is a common complication of sepsis, and has been associated with increased morbidity and mortality.. Nuclear factor of activated T cells (NFATs) 1, a transcriptional factor that regulates T cell development, activation and differentiation, has been implicated in neuronal plasticity.. Here we examined the potential role of NFAT1 in sepsis-associated encephalopathy in mice.

Random Hexamer:

Article Title: Prevalence and Risk Factors of Occult HCV Infection in the Adult Population of Mexico City.
Article Snippet: RNA from plasma and PBMCs was quantified on a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and adjusted to a 20 ng/μL concentration. .. The cDNA was obtained by reverse transcription using the AMV reverse transcriptase kit and random hexamer primers (Promega, Madison, WI, USA), following the manufacturer’s instructions. ..

Synthesized:

Article Title: The aryl hydrocarbon receptor controls mesenchymal stromal cell-mediated immunomodulation via ubiquitination of eukaryotic elongation factor-2 kinase
Article Snippet: After 24 h, MSCs were harvested to extract the total mRNA. mRNA was extracted from MSCs using the TRIzol reagent (Gibco). .. According to the manufacturer’s instructions, cDNA was synthesized using an AMV reverse transcriptase Kit (Promega, Madison, WI, USA). .. The cDNA products were amplified using the Kapa SYBR qPCR Kit master mix (2x) from Kapa Biosystems (Sigma-Aldrich). cDNA was amplified using primers specific for iNOS and β-actin (Qiagen, Hilden, Germany).

Article Title: T Cell-Induced Colitis Is Exacerbated by Prolonged Stress: A Comparison in Male and Female Mice
Article Snippet: Mid colons were snap frozen in liquid nitrogen and stored at −80 °C until isolating total RNA using Tri-zol reagent (Invitrogen, Carlsbad, CA, USA) following manufacturer protocols. .. Complimentary DNA was synthesized with the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA). .. All mouse mRNA primers were purchased from Integrated DNA Technologies (Redwood City, CA, USA).

Article Title: The Impact of Varying Alginate Co-activation with Probiotics on the Artemia Bioencapsulation to Enhance Immunity Against Vibrio spp.
Article Snippet: The total RNA was extracted from Artemia's whole body with TRIzol Reagent (Thermo Fisher Scientific) based on the procedure by the manufacturer. .. The total RNA was then synthesized to get cDNA using AMV Reverse Transcriptase Kit (Promega Corp.) at 42°C for 30 min, followed by transcriptase enzyme inactivated at 94°C for 5 min. .. The expression of proPO, LGBP, and lectin was analyzed relatively with an internal control using Sybr Green Master Mix (Kapa SYBER FAST qPCR Master Mix) (Kapa biosystems).

Virus:

Article Title: T Cell-Induced Colitis Is Exacerbated by Prolonged Stress: A Comparison in Male and Female Mice
Article Snippet: Mid colons were snap frozen in liquid nitrogen and stored at −80 °C until isolating total RNA using Tri-zol reagent (Invitrogen, Carlsbad, CA, USA) following manufacturer protocols. .. Complimentary DNA was synthesized with the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA). .. All mouse mRNA primers were purchased from Integrated DNA Technologies (Redwood City, CA, USA).



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