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reverse transcriptase kit  (Jena Bioscience)


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    Structured Review

    Jena Bioscience reverse transcriptase kit
    Reverse Transcriptase Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+kit/SCRIPT+Reverse+Transcriptase+Kit/pm42285501-335-19-22
    Average 94 stars, based on 26 article reviews
    reverse transcriptase kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Hypoxia impedes differentiation of cranial neural crest cells into derivatives relevant for craniofacial development.
    Article Snippet: Osteoblast differentiation medium was alpha-MEM (Gibco) containing 10% FCS, 1% penicillin/streptomycin, 10 mM beta-glycerophosphate, 0.1 μM dexamethasone and 100 ng ml−1 BMP-2. .. After reverse transcription with SCRIPT cDNA synthesis kit (Jena bioscience), quantitative reverse transcriptase PCR (qRT-PCR) was performed on a Roche LightCycler 96 with the primer pairs listed in electronic supplementary material, table S4. ..

    Article Title: Coordinated IFN-γ/TNF Axis Drives Selective Loss of Activated Enteric Glia in Inflammatory Bowel Diseases.
    Article Snippet: 653 Jo urn al Pr -pr oo f 22 AntiTUBB3 Biotinylated 1:100 Mouse 801212 BioLegend, Inc. AntiGFAP Alexa Flour 488 1:400 Mouse 644704 BioLegend, Inc. Anti-CD4 Unconjugated 1:100 Rat BD (553043) BD Biosciences AntipMLKL (S345) Unconjugated 1:400 Rabbit 37333S Cell Signaling Technologies Secondary antibody AntiRabbit Biotinylated 1:500 Goat 111-065-144 Jackson Anti-Rat Biotinylated 1:500 Goat 554014 BD Biosciences AntiRabbit Alexa Flour 647 1:400 Donkey 406414 BioLegend, Inc. Anti-Rat Dylight 550 1:400 Donkey SA5-10027 ThermoFisher Scientific GmbH FACS antibodies AntiGFAP Brilliant Violet 421 1:200 Mouse 644710 BioLegend, Inc. MHC II I-A/E-A FITC & APCCy7 1:2000 Rat 107606 & 107628 BioLegend, Inc. Western blot antibodies PLP1 Unconjugated 1:500 Mouse MCA839G BIO-RAD Jo urn al Pr e-p roo f 23 655 RNA isolation, reverse transcription, and qPCR 656 RNA was obtained from homogenized tissue or FACS sorted EGC with the column-based RNeasy 657 kit (Macherey-Nagel GmbH). .. After quality control via Nanodrop, 200 ng of the RNA was processed 658 into first strand cDNA using the reverse transcriptase kit (Jena Bioscience GmbH) according to 659 manufactures instructions. .. For qPCR, 2 μL of cDNA was used in a 20 μL SYBR Green based PCR 660 reactions in 96-well format, reaction performed in a BioRad PCR CFX Connect OM.

    Article Title: Regulation of long non-coding RNA NEAT1 by isoforms of transcription factor C/EBPα in acute myeloid leukemia
    Article Snippet: .. One microgram of total RNA was converted into complementary DNA (cDNA) using the SCRIPT Reverse Transcriptase kit from Jena Bioscience (PCR-505S, Jena Bioscience, Jena, Germany) and random hexamer primers. .. Quantitative real-time PCR was performed to quantify the expression levels of NEAT1.1 (thermofisher assayID: Hs03453535_s1; Cat#:4331182, Thermo Fisher Scientific, Waltham, USA) and NEAT1.2 (thermofisher assayID: Hs03924655_s1; Cat#: 4426961, Thermo Fisher Scien-tific, Waltham, USA).

    Article Title: Immunohistochemical analysis of YB-1 expression in the developing mouse eye
    Article Snippet: Briefly, tissue was homogenized in lysis buffer (Macherey and Nagel Nucleospin RNA/Protein kit), and RNA and protein was prepared according to the manufacturer’s recommendations. .. RNA was quantified by UV spectroscopy, and cDNA was synthesized from 500 ng using anchored oligo-dT-primer and Script reverse transcriptase, following the manufacturer’s (Jena Bioscience, Jena, Germany) manual. .. PCR was performed using YourTaq Direct-Load PCR Mix (biotechrabbit, Berlin, Germany) in 30 cycles (96°C for 20 s; 60°C for 20 s; 72°C for 1 min, with a final 10 min extension at 72°C) using the following primers (Eurofins MWG Operon, Ebersberg, Germany): fw1: GGAGAAGTGATG-GAGGGTGC; rev1: GGTTTAGGGTTCTCTGGGCG; fw2: AGAACGAGGGATCGGAAAGC; rev2: TGCCATCCTCTC-TAGGCTGT.

    cDNA Synthesis:

    Article Title: Hypoxia impedes differentiation of cranial neural crest cells into derivatives relevant for craniofacial development.
    Article Snippet: Osteoblast differentiation medium was alpha-MEM (Gibco) containing 10% FCS, 1% penicillin/streptomycin, 10 mM beta-glycerophosphate, 0.1 μM dexamethasone and 100 ng ml−1 BMP-2. .. After reverse transcription with SCRIPT cDNA synthesis kit (Jena bioscience), quantitative reverse transcriptase PCR (qRT-PCR) was performed on a Roche LightCycler 96 with the primer pairs listed in electronic supplementary material, table S4. ..

    Polymerase Chain Reaction:

    Article Title: Hypoxia impedes differentiation of cranial neural crest cells into derivatives relevant for craniofacial development.
    Article Snippet: Osteoblast differentiation medium was alpha-MEM (Gibco) containing 10% FCS, 1% penicillin/streptomycin, 10 mM beta-glycerophosphate, 0.1 μM dexamethasone and 100 ng ml−1 BMP-2. .. After reverse transcription with SCRIPT cDNA synthesis kit (Jena bioscience), quantitative reverse transcriptase PCR (qRT-PCR) was performed on a Roche LightCycler 96 with the primer pairs listed in electronic supplementary material, table S4. ..

    Article Title: Regulation of long non-coding RNA NEAT1 by isoforms of transcription factor C/EBPα in acute myeloid leukemia
    Article Snippet: .. One microgram of total RNA was converted into complementary DNA (cDNA) using the SCRIPT Reverse Transcriptase kit from Jena Bioscience (PCR-505S, Jena Bioscience, Jena, Germany) and random hexamer primers. .. Quantitative real-time PCR was performed to quantify the expression levels of NEAT1.1 (thermofisher assayID: Hs03453535_s1; Cat#:4331182, Thermo Fisher Scientific, Waltham, USA) and NEAT1.2 (thermofisher assayID: Hs03924655_s1; Cat#: 4426961, Thermo Fisher Scien-tific, Waltham, USA).

    other:

    Article Title: Disrupting Pathogenicity in Foodborne Staphylococcus aureus : Biofilm Inhibition and Attenuation of Resistance and Virulence by Tunisian Aromatic Plant Essential Oils.
    Article Snippet: RNA quality and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    QC/EQA/Proficiency:

    Article Title: Coordinated IFN-γ/TNF Axis Drives Selective Loss of Activated Enteric Glia in Inflammatory Bowel Diseases.
    Article Snippet: 653 Jo urn al Pr -pr oo f 22 AntiTUBB3 Biotinylated 1:100 Mouse 801212 BioLegend, Inc. AntiGFAP Alexa Flour 488 1:400 Mouse 644704 BioLegend, Inc. Anti-CD4 Unconjugated 1:100 Rat BD (553043) BD Biosciences AntipMLKL (S345) Unconjugated 1:400 Rabbit 37333S Cell Signaling Technologies Secondary antibody AntiRabbit Biotinylated 1:500 Goat 111-065-144 Jackson Anti-Rat Biotinylated 1:500 Goat 554014 BD Biosciences AntiRabbit Alexa Flour 647 1:400 Donkey 406414 BioLegend, Inc. Anti-Rat Dylight 550 1:400 Donkey SA5-10027 ThermoFisher Scientific GmbH FACS antibodies AntiGFAP Brilliant Violet 421 1:200 Mouse 644710 BioLegend, Inc. MHC II I-A/E-A FITC & APCCy7 1:2000 Rat 107606 & 107628 BioLegend, Inc. Western blot antibodies PLP1 Unconjugated 1:500 Mouse MCA839G BIO-RAD Jo urn al Pr e-p roo f 23 655 RNA isolation, reverse transcription, and qPCR 656 RNA was obtained from homogenized tissue or FACS sorted EGC with the column-based RNeasy 657 kit (Macherey-Nagel GmbH). .. After quality control via Nanodrop, 200 ng of the RNA was processed 658 into first strand cDNA using the reverse transcriptase kit (Jena Bioscience GmbH) according to 659 manufactures instructions. .. For qPCR, 2 μL of cDNA was used in a 20 μL SYBR Green based PCR 660 reactions in 96-well format, reaction performed in a BioRad PCR CFX Connect OM.

    Random Hexamer:

    Article Title: Regulation of long non-coding RNA NEAT1 by isoforms of transcription factor C/EBPα in acute myeloid leukemia
    Article Snippet: .. One microgram of total RNA was converted into complementary DNA (cDNA) using the SCRIPT Reverse Transcriptase kit from Jena Bioscience (PCR-505S, Jena Bioscience, Jena, Germany) and random hexamer primers. .. Quantitative real-time PCR was performed to quantify the expression levels of NEAT1.1 (thermofisher assayID: Hs03453535_s1; Cat#:4331182, Thermo Fisher Scientific, Waltham, USA) and NEAT1.2 (thermofisher assayID: Hs03924655_s1; Cat#: 4426961, Thermo Fisher Scien-tific, Waltham, USA).

    Spectroscopy:

    Article Title: Immunohistochemical analysis of YB-1 expression in the developing mouse eye
    Article Snippet: Briefly, tissue was homogenized in lysis buffer (Macherey and Nagel Nucleospin RNA/Protein kit), and RNA and protein was prepared according to the manufacturer’s recommendations. .. RNA was quantified by UV spectroscopy, and cDNA was synthesized from 500 ng using anchored oligo-dT-primer and Script reverse transcriptase, following the manufacturer’s (Jena Bioscience, Jena, Germany) manual. .. PCR was performed using YourTaq Direct-Load PCR Mix (biotechrabbit, Berlin, Germany) in 30 cycles (96°C for 20 s; 60°C for 20 s; 72°C for 1 min, with a final 10 min extension at 72°C) using the following primers (Eurofins MWG Operon, Ebersberg, Germany): fw1: GGAGAAGTGATG-GAGGGTGC; rev1: GGTTTAGGGTTCTCTGGGCG; fw2: AGAACGAGGGATCGGAAAGC; rev2: TGCCATCCTCTC-TAGGCTGT.

    Synthesized:

    Article Title: Immunohistochemical analysis of YB-1 expression in the developing mouse eye
    Article Snippet: Briefly, tissue was homogenized in lysis buffer (Macherey and Nagel Nucleospin RNA/Protein kit), and RNA and protein was prepared according to the manufacturer’s recommendations. .. RNA was quantified by UV spectroscopy, and cDNA was synthesized from 500 ng using anchored oligo-dT-primer and Script reverse transcriptase, following the manufacturer’s (Jena Bioscience, Jena, Germany) manual. .. PCR was performed using YourTaq Direct-Load PCR Mix (biotechrabbit, Berlin, Germany) in 30 cycles (96°C for 20 s; 60°C for 20 s; 72°C for 1 min, with a final 10 min extension at 72°C) using the following primers (Eurofins MWG Operon, Ebersberg, Germany): fw1: GGAGAAGTGATG-GAGGGTGC; rev1: GGTTTAGGGTTCTCTGGGCG; fw2: AGAACGAGGGATCGGAAAGC; rev2: TGCCATCCTCTC-TAGGCTGT.



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