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hiscript reverse transcriptase kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript reverse transcriptase kit
    Hiscript Reverse Transcriptase Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 5357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+kit/HiScript+III+1st+Strand+cDNA+Synthesis+Kit+%2BgDNA+wiper/pm42816486-356-13-17
    Average 99 stars, based on 5357 article reviews
    hiscript reverse transcriptase kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Targeting SUV39H2 exon skipping reverses resistance to CDK4/6 inhibitors via transcriptional activation of p21
    Article Snippet: RNA quality and concentration were detected with a NanoDrop 2000 (Invitrogen). .. RNA was reverse-transcribed to cDNA using HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, R312-01). .. RT-qPCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02).

    Article Title: α-KG conformationally stabilizes nuclear GLUD1-SNAIL complex to fuel trans-endothelial migration in metastasis.
    Article Snippet: Quantify tube formation using Fiji (ImageJ) with the Angiogenesis Analyzer2 plugin to automatically assess endothelial network parameters (e.g., tube length, branching points). .. Total RNA was isolated using TRIzol Reagent, followed by cDNA synthesis with the HiScript Reverse Transcriptase kit (Vazyme). .. Quantitative PCR was performed on a CFX96 Real-Time System (BioRad) using SYBR Green PCR Master Mix (Bio-Rad) under the following conditions: 95 °C for 15 sec (denaturation), 60 °C for 30 sec (annealing/ extension) for 40 cycles.

    Article Title: Heterologous expression of Xanthophyllomyces dendrorhous crtS/crtR in Escherichia coli for preliminary astaxanthin formation.
    Article Snippet: .. Total RNA was extracted with Trizol reagent (Sangon Biotech, B511311-0025), reverse transcription was performed using HiScript III RT SuperMix for qPCR (Vazyme, R312-01), and seamless cloning was performed using ClonExpress Ultra One Step Cloning Kit (Vazyme, C115). .. β-Carotene standard (B21973) and astaxanthin standard (B45743) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd.

    Article Title: Bio-NR1@ZIF8 Ameliorate Pulpitis Via Alleviating RSAD2-Mediated Mitochondrial Dysfunction
    Article Snippet: Total RNA was harvested from the hDPSCs utilising the TRIzol-mediated extraction protocol (Invitrogen Life Technologies). .. Subsequently, the isolated RNA templates were converted into complementary DNA (cDNA) using a HiScript Reverse Transcription Kit (Vazyme Biotech) following the manufacturer’s instructions. .. The quantitative amplification was executed on a real-time PCR system using the SYBR Green Master Mix (Vazyme).

    cDNA Synthesis:

    Article Title: Targeting SUV39H2 exon skipping reverses resistance to CDK4/6 inhibitors via transcriptional activation of p21
    Article Snippet: RNA quality and concentration were detected with a NanoDrop 2000 (Invitrogen). .. RNA was reverse-transcribed to cDNA using HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, R312-01). .. RT-qPCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02).

    Article Title: α-KG conformationally stabilizes nuclear GLUD1-SNAIL complex to fuel trans-endothelial migration in metastasis.
    Article Snippet: Quantify tube formation using Fiji (ImageJ) with the Angiogenesis Analyzer2 plugin to automatically assess endothelial network parameters (e.g., tube length, branching points). .. Total RNA was isolated using TRIzol Reagent, followed by cDNA synthesis with the HiScript Reverse Transcriptase kit (Vazyme). .. Quantitative PCR was performed on a CFX96 Real-Time System (BioRad) using SYBR Green PCR Master Mix (Bio-Rad) under the following conditions: 95 °C for 15 sec (denaturation), 60 °C for 30 sec (annealing/ extension) for 40 cycles.

    Article Title: Chromatin interaction landscape of epimorphic regeneration in teleost fish
    Article Snippet: .. The cDNA of knockout transgenic killifish lines was prepared using the HiScript III first-strand cDNA Synthesis Kit (Vazyme, R312). .. Quantitative real-time PCR was performed using Taq Pro Universal SYBR quantitative PCR (qPCR) Master Mix (Vazyme, Q712) according to the manufacturer’s instructions.

    Article Title: From genome to gene module: decoding the BsAlfin2-BsTT2 regulatory network controlling low temperature-induced anthocyanin biosynthesis in Begonia semperflorens
    Article Snippet: Total RNA using FastPure Universal Plant Total RNA Isolation Kit (Vazyme, China). .. DNA-free RNA was used to synthesize the first strand of cDNA with the HiScript III 1st Strand cDNA Synthesis Kit (Vazyme, China). ..

    Isolation:

    Article Title: α-KG conformationally stabilizes nuclear GLUD1-SNAIL complex to fuel trans-endothelial migration in metastasis.
    Article Snippet: Quantify tube formation using Fiji (ImageJ) with the Angiogenesis Analyzer2 plugin to automatically assess endothelial network parameters (e.g., tube length, branching points). .. Total RNA was isolated using TRIzol Reagent, followed by cDNA synthesis with the HiScript Reverse Transcriptase kit (Vazyme). .. Quantitative PCR was performed on a CFX96 Real-Time System (BioRad) using SYBR Green PCR Master Mix (Bio-Rad) under the following conditions: 95 °C for 15 sec (denaturation), 60 °C for 30 sec (annealing/ extension) for 40 cycles.

    Article Title: Bio-NR1@ZIF8 Ameliorate Pulpitis Via Alleviating RSAD2-Mediated Mitochondrial Dysfunction
    Article Snippet: Total RNA was harvested from the hDPSCs utilising the TRIzol-mediated extraction protocol (Invitrogen Life Technologies). .. Subsequently, the isolated RNA templates were converted into complementary DNA (cDNA) using a HiScript Reverse Transcription Kit (Vazyme Biotech) following the manufacturer’s instructions. .. The quantitative amplification was executed on a real-time PCR system using the SYBR Green Master Mix (Vazyme).

    Knock-Out:

    Article Title: Chromatin interaction landscape of epimorphic regeneration in teleost fish
    Article Snippet: .. The cDNA of knockout transgenic killifish lines was prepared using the HiScript III first-strand cDNA Synthesis Kit (Vazyme, R312). .. Quantitative real-time PCR was performed using Taq Pro Universal SYBR quantitative PCR (qPCR) Master Mix (Vazyme, Q712) according to the manufacturer’s instructions.

    Transgenic Assay:

    Article Title: Chromatin interaction landscape of epimorphic regeneration in teleost fish
    Article Snippet: .. The cDNA of knockout transgenic killifish lines was prepared using the HiScript III first-strand cDNA Synthesis Kit (Vazyme, R312). .. Quantitative real-time PCR was performed using Taq Pro Universal SYBR quantitative PCR (qPCR) Master Mix (Vazyme, Q712) according to the manufacturer’s instructions.

    Synthesized:

    Article Title: Extremely low light promotes adventitious shoot regeneration via RpPHYA/RpHYH-antagonized auxin biosynthesis and transport through dual regulation of RpPIN1 in Robinia pseudoacacia
    Article Snippet: .. Genomic DNA (DC104-01, Vazyme) and total RNA (RC411-01, Vazyme) were extracted, and first-strand cDNA (R312-01, Vazyme) was synthesized. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Heterologous expression of Xanthophyllomyces dendrorhous crtS/crtR in Escherichia coli for preliminary astaxanthin formation.
    Article Snippet: .. Total RNA was extracted with Trizol reagent (Sangon Biotech, B511311-0025), reverse transcription was performed using HiScript III RT SuperMix for qPCR (Vazyme, R312-01), and seamless cloning was performed using ClonExpress Ultra One Step Cloning Kit (Vazyme, C115). .. β-Carotene standard (B21973) and astaxanthin standard (B45743) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd.

    Cloning:

    Article Title: Heterologous expression of Xanthophyllomyces dendrorhous crtS/crtR in Escherichia coli for preliminary astaxanthin formation.
    Article Snippet: .. Total RNA was extracted with Trizol reagent (Sangon Biotech, B511311-0025), reverse transcription was performed using HiScript III RT SuperMix for qPCR (Vazyme, R312-01), and seamless cloning was performed using ClonExpress Ultra One Step Cloning Kit (Vazyme, C115). .. β-Carotene standard (B21973) and astaxanthin standard (B45743) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd.



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