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reverse transcriptase enzyme improm-ii reserve transcription system  (Promega)

 
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    Promega reverse transcriptase enzyme improm-ii reserve transcription system
    Reverse Transcriptase Enzyme Improm Ii Reserve Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/reverse+transcriptase+enzyme+improm+ii+reserve+transcription+system/pm21422666-97-13-20
    Average 90 stars, based on 1 article reviews
    reverse transcriptase enzyme improm-ii reserve transcription system - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Differentiation of MC3T3-E1 on poly(4-styrenesulfonic acid-co-maleic acid)sodium salt-coated films.
    Article Snippet: RNA yields were evaluated with a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) based on the absorbance ratio at 260/280 nm. .. First strand DNA was reverse transcribed from 1 μg of total RNA using reverse transcriptase enzyme (ImProm-II Reserve Transcription System, Promega, Madison, WI, USA). qPCR was performed using the LightCycler 480 (Roche, Mannheim, Germany) and LightCycler® SYBR Green I Master (Roche, Mannheim, Germany) in a 10 μL reaction volume under the following cycling conditions: 95°C, 5 min, followed by 40 cycles of 95°C for 10 s; 60°C for 10 s: 72°C for 25s. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Differentiation of MC3T3-E1 on poly(4-styrenesulfonic acid-co-maleic acid)sodium salt-coated films.
    Article Snippet: RNA yields were evaluated with a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) based on the absorbance ratio at 260/280 nm. .. First strand DNA was reverse transcribed from 1 μg of total RNA using reverse transcriptase enzyme (ImProm-II Reserve Transcription System, Promega, Madison, WI, USA). qPCR was performed using the LightCycler 480 (Roche, Mannheim, Germany) and LightCycler® SYBR Green I Master (Roche, Mannheim, Germany) in a 10 μL reaction volume under the following cycling conditions: 95°C, 5 min, followed by 40 cycles of 95°C for 10 s; 60°C for 10 s: 72°C for 25s. ..

    SYBR Green Assay:

    Article Title: Differentiation of MC3T3-E1 on poly(4-styrenesulfonic acid-co-maleic acid)sodium salt-coated films.
    Article Snippet: RNA yields were evaluated with a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) based on the absorbance ratio at 260/280 nm. .. First strand DNA was reverse transcribed from 1 μg of total RNA using reverse transcriptase enzyme (ImProm-II Reserve Transcription System, Promega, Madison, WI, USA). qPCR was performed using the LightCycler 480 (Roche, Mannheim, Germany) and LightCycler® SYBR Green I Master (Roche, Mannheim, Germany) in a 10 μL reaction volume under the following cycling conditions: 95°C, 5 min, followed by 40 cycles of 95°C for 10 s; 60°C for 10 s: 72°C for 25s. ..



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    a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of <t>transcription</t> factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.
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    Image Search Results


    a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of transcription factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Dyna-vivo-seq unveils cellular RNA dynamics during acute kidney injury via in vivo metabolic RNA labeling-based scRNA-seq

    doi: 10.1038/s41467-024-54202-4

    Figure Lengend Snippet: a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of transcription factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.

    Article Snippet: The barcoded beads were then resuspended in a reverse transcription mix, consisting of 1× RT buffer, 1 mM dNTP (TransGen Biotech, cat# AD101-12), 2.5 μM Template Switch Oligo (Sangon, 5’-AAGCAGTGGTATCAACGCAGAGTGAATrGrGrG-3’), 1U/μL Recombination RNase Inhibitor (TaKaRa, cat# 2313 A), 10 U/μL reverse transcription enzyme (Thermo Fisher, cat# EP0753), 1 mM GTP (Thermo Fisher, cat# R0461), and 5% PEG-8000 (Beyotime, cat# R0056).

    Techniques: Labeling, In Vivo, Expressing, Activity Assay