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mouse llc  (ATCC)


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  • 96

    Structured Review

    ATCC mouse llc
    KEY RESOURCES TABLE
    Mouse Llc, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/representative+tissue+microarray+staining/LL%2F2+(LLC1)%3B+Lewis+Lung+Carcinoma%3B+Mouse/pmc06719696-87-0-3
    Average 96 stars, based on 282 article reviews
    mouse llc - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer"

    Article Title: Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer

    Journal: Cell reports

    doi: 10.1016/j.celrep.2019.03.066

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Flow Cytometry, Purification, In Vitro, Activation Assay, In Vivo, Control, Virus, Plasmid Preparation, Recombinant, Staining, Luminex, Multiplex Assay, Cell Viability Assay, Gene Expression, Transgenic Assay, Software, Imaging, Microarray

    Related Articles

    Expressing:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Marker:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Cell Culture:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Activity Assay:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Concentration Assay:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Comparison:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Control:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Hydroxyproline Assay:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    SYBR Green Assay:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Labeling:

    Article Title: Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclasts
    Article Snippet: Reagent Form Catalog # Company Location TRAP 5b Mouse TRAPTM (TRAP 5b) ELISA Kit Cat #: SB-TR103 Immunodiagnostic Systems United Kingdom IL-1β Mouse IL-1 beta DuoSet ELISA Kit Cat #: DY40105 R&D Systems Inc Minneapolis, MN CRP Mouse C-Reactive Protein DuoSet ELISA Kit Cat #: DY1829 R&D Systems Inc Minneapolis, MN RANKL Mouse RANK L/TNFSF11 Quantikine ELISA Kit Cat #: MTR00 R&D Systems Inc Minneapolis, MN OPG Mouse OPG/TNFRSF11B ELISA Kit Cat #: DY459 R&D Systems Inc Minneapolis, MN M-CSF Mouse M-CSF DuoSet ELISA Kit Cat #: DY416 R&D Systems Inc Minneapolis, MN anti-mouse RANKL Monoclonal antibody Cat #:BE0191, Clone IK 22-5 Bio-X-Cell West Lebanon, NH Rat Ig2a Isotype control IgG Cat #: BE0089, Clone 2A-3 Bio-X-Cell West Lebanon, NH anti-Rabbit IgG Secondary antibody Cat # 7074V Cell Signaling Danvers, MA Hydroxyproline Assay kit Cat #: QZBhypro5 QuickZyme biosciences Leiden, Netherlands Type I collagenase Enzyme from Clostridium histolyticum Cat #: AAJ62406MC Thermo Scientific Rockford, IL RNAzol RT RNA preparation Cat #: RN 190 Molecular Research Ctr Cincinnati, OH SYBR Green Master Mix for rtPCR Cat #: A25777 Applied Biosystems Beverly, MA High-capacity cDNA prep RT kit Cat #: 43-688-14 Applied Biosystems Beverly, MA Endotoxin LAL Chromogenic Quant Kit Cat #: A39552 Thermo Scientific Rockford, IL Lewis Lung Ca cells LL2-DS-Red labeled Cat #: ATCC CRL-1642 Gift, Dr. Palumbo, CCHMC Cincinnati, OH Steritalc Graded mineral particle NDC-62327-444-44 Boston Medical Boston, MA Talc Ungraded mineral particle Cat #: T2-500 ThermoFisher Fairlawn, N.J. Hydroxyapatite Particle nanoXIM•HAp202 CAS No.1306-06-5 Fluidinova Portugal Hydroxyapatite Spherical particles CER-0007 Matexcel Shirley, N.J.


    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).



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    Proteintech representative tissue microarray staining
    Fig. 9. Reduced emerin expression at the nuclear periphery correlates with breast cancer invasiveness in patients. (A) Representative tissue <t>microarray</t> staining of emerin in 159 patients using emerin polyclonal antibodies (Proteintech, cat# 10351-1-AP) or secondary alone (Vector Lab, cat#: MP-7451). Nuclei are blue, emerin is brown, and arrows denote emerin staining in certain images for reference. As severity of cases increases, there is a visible reduction in emerin expression at the nuclear envelope and more deformed nuclei are present. (B) Quantification of emerin staining on IHC-stained patient samples using 0–3, with 0 having no staining at the nuclear periphery and 3 having complete, dark rim staining. N = 159 total samples, *P < 0.05 compared to normal tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation. (C) Representative tissue microarray staining of emerin in 183 patients using emerin monoclonal antibodies (Leica, NCL-Emerin) or secondary alone (Vector Lab, cat#: MP-7452) using the same samples used in A. Nuclei are blue and emerin is brown. As aggressiveness of cases increases, there is a visible reduction in emerin expression and more deformed nuclei are present. (D) Quantification of emerin staining using the 0 to 3 grading system. N = 183 total samples #P < 0.02 compared to all non-cancerous tissue, *P < 0.0062 compared to both normal and benign tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation.
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    Fig. 9. Reduced emerin expression at the nuclear periphery correlates with breast cancer invasiveness in patients. (A) Representative tissue microarray staining of emerin in 159 patients using emerin polyclonal antibodies (Proteintech, cat# 10351-1-AP) or secondary alone (Vector Lab, cat#: MP-7451). Nuclei are blue, emerin is brown, and arrows denote emerin staining in certain images for reference. As severity of cases increases, there is a visible reduction in emerin expression at the nuclear envelope and more deformed nuclei are present. (B) Quantification of emerin staining on IHC-stained patient samples using 0–3, with 0 having no staining at the nuclear periphery and 3 having complete, dark rim staining. N = 159 total samples, *P < 0.05 compared to normal tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation. (C) Representative tissue microarray staining of emerin in 183 patients using emerin monoclonal antibodies (Leica, NCL-Emerin) or secondary alone (Vector Lab, cat#: MP-7452) using the same samples used in A. Nuclei are blue and emerin is brown. As aggressiveness of cases increases, there is a visible reduction in emerin expression and more deformed nuclei are present. (D) Quantification of emerin staining using the 0 to 3 grading system. N = 183 total samples #P < 0.02 compared to all non-cancerous tissue, *P < 0.0062 compared to both normal and benign tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation.

    Journal: Scientific reports

    Article Title: Emerin deficiency drives MCF7 cells to an invasive phenotype.

    doi: 10.1038/s41598-024-70752-5

    Figure Lengend Snippet: Fig. 9. Reduced emerin expression at the nuclear periphery correlates with breast cancer invasiveness in patients. (A) Representative tissue microarray staining of emerin in 159 patients using emerin polyclonal antibodies (Proteintech, cat# 10351-1-AP) or secondary alone (Vector Lab, cat#: MP-7451). Nuclei are blue, emerin is brown, and arrows denote emerin staining in certain images for reference. As severity of cases increases, there is a visible reduction in emerin expression at the nuclear envelope and more deformed nuclei are present. (B) Quantification of emerin staining on IHC-stained patient samples using 0–3, with 0 having no staining at the nuclear periphery and 3 having complete, dark rim staining. N = 159 total samples, *P < 0.05 compared to normal tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation. (C) Representative tissue microarray staining of emerin in 183 patients using emerin monoclonal antibodies (Leica, NCL-Emerin) or secondary alone (Vector Lab, cat#: MP-7452) using the same samples used in A. Nuclei are blue and emerin is brown. As aggressiveness of cases increases, there is a visible reduction in emerin expression and more deformed nuclei are present. (D) Quantification of emerin staining using the 0 to 3 grading system. N = 183 total samples #P < 0.02 compared to all non-cancerous tissue, *P < 0.0062 compared to both normal and benign tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation.

    Article Snippet: Reduced emerin expression at the nuclear periphery correlates with breast cancer invasiveness in patients. (A) Representative tissue microarray staining of emerin in 159 patients using emerin polyclonal antibodies (Proteintech, cat# 10351-1-AP) or secondary alone (Vector Lab, cat#: MP-7451).

    Techniques: Expressing, Microarray, Staining, Plasmid Preparation, Standard Deviation, Bioprocessing