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anti timp 1 antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology anti timp 1 antibody
    Anti Timp 1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/representative+tissue+microarray+staining/TIMP-1+Antibody/pm15023835-62-24-27
    Average 96 stars, based on 692 article reviews
    anti timp 1 antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: The TRAF6/SPP1 axis participates in osteoarthritis progression through regulating the catabolism and anabolism of cartilage matrix.
    Article Snippet: The tissue inhibitor of metalloproteinase 1 (TIMP1) (sc-21734, Santa Cruz, California, USA) antibody was procured from Santa Cruz Biotechnology.

    Article Title: CD44/POU2F2/BCL9L axis mediates MIF-driven SPP1 + TAM activation in colorectal cancer metastasis
    Article Snippet: Primary antibodies including anti-SPP1, anti-MARCO, anti-MIF, anti-POU2F2, anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-MMP2, anti-GAPDH, anti-TIMP-1, anti-CD74, anti-CD44, anti-POU2F2 and anti-CXCR4 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Staining:

    Article Title: Physalin A Suppresses Human Oral Squamous Carcinoma Cell Migration and Invasion Through Inhibiting Grb2/Ras and MMP/uPA Signaling Pathways.
    Article Snippet: The quantified proteins were electrophoretically separated by 8-12% SDSPAGE gel (based on different KDa), and were transferred to polyvinylidene difluoride (PVDF; Cat. No. IPVH00010; Merck Millipore, Burlington, MA, USA) membranes, which were blocked with 5% albumin. .. The membranes were then stained using primary antibodies [anti- plasminogen activator inhibitor 1 (PAI-1; sc-5297; Santa Cruz Biotechnology), -uPA (sc-59727; Santa Cruz Biotechnology), -E-cad (sc-374067; Santa Cruz Biotechnology), -α-tubulin (α-tub; sc-8035; Santa Cruz Biotechnology), -MMP-1 (sc21731; Santa Cruz Biotechnology), -MMP-2 (sc-13595; Santa Cruz Biotechnology), -MMP-3 (sc-21732; Santa Cruz Biotechnology), -MMP-9 (sc-393859; Santa Cruz Biotechnology), -tissue inhibitor of metalloprotease (TIMP1; sc-365905; Santa Cruz Biotechnology), -TIMP-2 (sc21735; Santa Cruz Biotechnology), -p-IKK (p-IKK; sc293135; Santa Cruz Biotechnology), -IKK (sc-8032; Santa Cruz Biotechnology), -p-IκB (sc-8404; Santa Cruz Biotechnology), -IκB (sc-1643; Santa Cruz Biotechnology), -NF-κB p65 (sc-8008; Santa Cruz Biotechnology), -NF-κB p50 (sc-8414; Santa Cruz Biotechnology), -p-ERK (9911; Cell Signaling Technology), -ERK (9911; Cell Signaling Technology), -p-c-Jun N-terminal kinase (-p-JNK; 4668; Cell Signaling Technology), -JNK (9252; Cell Signaling Technology), -p-p38 (9211; Cell Signaling Technology), -p38 (9212; Cell Signaling Technology), -p-c-Jun (3270; Cell Signaling Technology), -c-Jun (9165; Cell Signaling Technology), -Grb2 (3972; Cell Signaling Technology), -Ras (3965; Cell Signaling Technology), -p-PI3K (4228; Cell Signaling Technology), -PI3K (4292; Cell Signaling Technology), -p-Akt (9271; Cell Signaling Technology), -Akt (9272; Cell Signaling Technology), -PCNA (sc-56; Santa Cruz Biotechnology) and -β-actin (sc-47778; Santa Cruz Biotechnology)] in 4°C overnight. ..

    Article Title: Physalin A Suppresses Human Oral Squamous Carcinoma Cell Migration and Invasion Through Inhibiting Grb2/Ras and MMP/uPA Signaling Pathways
    Article Snippet: The quantified proteins were electrophoretically separated by 8-12% SDS-PAGE gel (based on different KDa), and were transferred to polyvinylidene difluoride (PVDF; Cat. No. IPVH00010; Merck Millipore, Burlington, MA, USA) membranes, which were blocked with 5% albumin. .. The membranes were then stained using primary antibodies [anti- plasminogen activator inhibitor 1 (PAI-1; sc-5297; Santa Cruz Biotechnology), -uPA (sc-59727; Santa Cruz Biotechnology), -E-cad (sc-374067; Santa Cruz Biotechnology), -α-tubulin (α-tub; sc-8035; Santa Cruz Biotechnology), -MMP-1 (sc-21731; Santa Cruz Biotechnology), -MMP-2 (sc-13595; Santa Cruz Biotechnology), -MMP-3 (sc-21732; Santa Cruz Biotechnology), -MMP-9 (sc-393859; Santa Cruz Biotechnology), -tissue inhibitor of metalloprotease (TIMP-1; sc-365905; Santa Cruz Biotechnology), -TIMP-2 (sc-21735; Santa Cruz Biotechnology), -p-IKK (p-IKK; sc-293135; Santa Cruz Biotechnology), -IKK (sc-8032; Santa Cruz Biotechnology), -p-IκB (sc-8404; Santa Cruz Biotechnology), -IκB (sc-1643; Santa Cruz Biotechnology), -NF-κB p65 (sc-8008; Santa Cruz Biotechnology), -NF-κB p50 (sc-8414; Santa Cruz Biotechnology), -p-ERK (9911; Cell Signaling Technology), -ERK (9911; Cell Signaling Technology), -p-c-Jun N-terminal kinase (-p-JNK; 4668; Cell Signaling Technology), -JNK (9252; Cell Signaling Technology), -p-p38 (9211; Cell Signaling Technology), -p38 (9212; Cell Signaling Technology), -p-c-Jun (3270; Cell Signaling Technology), -c-Jun (9165; Cell Signaling Technology), -Grb2 (3972; Cell Signaling Technology), -Ras (3965; Cell Signaling Technology), -p-PI3K (4228; Cell Signaling Technology), -PI3K (4292; Cell Signaling Technology), -p-Akt (9271; Cell Signaling Technology), -Akt (9272; Cell Signaling Technology), -PCNA (sc-56; Santa Cruz Biotechnology) and -β-actin (sc-47778; Santa Cruz Biotechnology)] in 4°C overnight. ..

    Incubation:

    Article Title: Kaempferia parviflora Extract Stabilizes Cartilage Homeostasis via TIMP-1-Associated Matrix Modulation in Monosodium Iodoacetate-Induced Rat Osteoarthritis.
    Article Snippet: .. Membranes were blocked with 5% skim milk in TBST (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween-20) for 1 h at room temperature and then incubated overnight at 4 ◦C with the following primary antibodies (1:500–1:1000 in TBST containing 5% skim milk): antiMMP1 (A306151; Abcam, Cambridge, UK), anti-MMP3 (14351; Cell Signaling Technology, Danvers, MA, USA), anti-MMP13 (ab39012; Abcam, Cambridge, UK), anti-TIMP-1 (sc21734; Santa Cruz Biotechnology, Dallas, TX, USA), and anti-β-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA). .. After washing, membranes were incubated for 1 h at room temperature with HRP-conjugated secondary antibodies (1:5000–1:10,000; anti-rabbit IgG-HRP, #7074; anti-mouse IgG-HRP, #7076; Cell Signaling Technology).

    Article Title: Kaempferia parviflora Extract Stabilizes Cartilage Homeostasis via TIMP-1–Associated Matrix Modulation in Monosodium Iodoacetate–Induced Rat Osteoarthritis
    Article Snippet: .. Membranes were blocked with 5% skim milk in TBST (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween-20) for 1 h at room temperature and then incubated overnight at 4 °C with the following primary antibodies (1:500–1:1000 in TBST containing 5% skim milk): anti-MMP1 (A306151; Abcam, Cambridge, UK), anti-MMP3 (14351; Cell Signaling Technology, Danvers, MA, USA), anti-MMP13 (ab39012; Abcam, Cambridge, UK), anti-TIMP-1 (sc-21734; Santa Cruz Biotechnology, Dallas, TX, USA), and anti-β-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA). .. After washing, membranes were incubated for 1 h at room temperature with HRP-conjugated secondary antibodies (1:5000–1:10,000; anti-rabbit IgG-HRP, #7074; anti-mouse IgG-HRP, #7076; Cell Signaling Technology).

    Bioprocessing:

    Article Title: Complex I Modulator BI4500 Reduces MASH by Limiting Oxidative Stress and Reprogramming Lipid Metabolism via AMPK in MCD Rats
    Article Snippet: .. Primary Antibody Secondary Antibody Mouse monoclonal antibodies ADAM-10 (Santa Cruz Biotechnology, Inc.) 1:200 1:1000 Desmin (Santa Cruz Biotechnology, Inc.) 1:5000 1:2000 HDAC (Cell Signaling Technology) 1:1000 1:2000 MMP-2 (Thermo Scientific) 1:200 1:1000 NFκB (Santa Cruz Biotechnology, Inc.) 1:1000 1:1000 PPARα (Santa Cruz Biotechnology, Inc.) 1:1000 1:1000 SREBP-1c (Santa Cruz Biotechnology, Inc.) 1:500 1:1000 TIMP-1 (Santa Cruz Biotechnology, Inc.) 1:500 1:1000 Tubulin (Merck Millipore) 1:1000 1:2000 Vinculin (Santa Cruz Biotechnology, Inc.) 1:1000 1:2000 https://doi.org/10.3390/antiox15010082 Table A2. ..



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    representative tissue microarray staining  (Proteintech)
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    Proteintech representative tissue microarray staining
    Fig. 9. Reduced emerin expression at the nuclear periphery correlates with breast cancer invasiveness in patients. (A) Representative tissue <t>microarray</t> staining of emerin in 159 patients using emerin polyclonal antibodies (Proteintech, cat# 10351-1-AP) or secondary alone (Vector Lab, cat#: MP-7451). Nuclei are blue, emerin is brown, and arrows denote emerin staining in certain images for reference. As severity of cases increases, there is a visible reduction in emerin expression at the nuclear envelope and more deformed nuclei are present. (B) Quantification of emerin staining on IHC-stained patient samples using 0–3, with 0 having no staining at the nuclear periphery and 3 having complete, dark rim staining. N = 159 total samples, *P < 0.05 compared to normal tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation. (C) Representative tissue microarray staining of emerin in 183 patients using emerin monoclonal antibodies (Leica, NCL-Emerin) or secondary alone (Vector Lab, cat#: MP-7452) using the same samples used in A. Nuclei are blue and emerin is brown. As aggressiveness of cases increases, there is a visible reduction in emerin expression and more deformed nuclei are present. (D) Quantification of emerin staining using the 0 to 3 grading system. N = 183 total samples #P < 0.02 compared to all non-cancerous tissue, *P < 0.0062 compared to both normal and benign tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation.
    Representative Tissue Microarray Staining, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/representative+tissue+microarray+staining/Emerin+Antibody/pm39198511-175-15-28
    Average 94 stars, based on 1 article reviews
    representative tissue microarray staining - by Bioz Stars, 2026-09
    94/100 stars
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    Fig. 9. Reduced emerin expression at the nuclear periphery correlates with breast cancer invasiveness in patients. (A) Representative tissue microarray staining of emerin in 159 patients using emerin polyclonal antibodies (Proteintech, cat# 10351-1-AP) or secondary alone (Vector Lab, cat#: MP-7451). Nuclei are blue, emerin is brown, and arrows denote emerin staining in certain images for reference. As severity of cases increases, there is a visible reduction in emerin expression at the nuclear envelope and more deformed nuclei are present. (B) Quantification of emerin staining on IHC-stained patient samples using 0–3, with 0 having no staining at the nuclear periphery and 3 having complete, dark rim staining. N = 159 total samples, *P < 0.05 compared to normal tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation. (C) Representative tissue microarray staining of emerin in 183 patients using emerin monoclonal antibodies (Leica, NCL-Emerin) or secondary alone (Vector Lab, cat#: MP-7452) using the same samples used in A. Nuclei are blue and emerin is brown. As aggressiveness of cases increases, there is a visible reduction in emerin expression and more deformed nuclei are present. (D) Quantification of emerin staining using the 0 to 3 grading system. N = 183 total samples #P < 0.02 compared to all non-cancerous tissue, *P < 0.0062 compared to both normal and benign tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation.

    Journal: Scientific reports

    Article Title: Emerin deficiency drives MCF7 cells to an invasive phenotype.

    doi: 10.1038/s41598-024-70752-5

    Figure Lengend Snippet: Fig. 9. Reduced emerin expression at the nuclear periphery correlates with breast cancer invasiveness in patients. (A) Representative tissue microarray staining of emerin in 159 patients using emerin polyclonal antibodies (Proteintech, cat# 10351-1-AP) or secondary alone (Vector Lab, cat#: MP-7451). Nuclei are blue, emerin is brown, and arrows denote emerin staining in certain images for reference. As severity of cases increases, there is a visible reduction in emerin expression at the nuclear envelope and more deformed nuclei are present. (B) Quantification of emerin staining on IHC-stained patient samples using 0–3, with 0 having no staining at the nuclear periphery and 3 having complete, dark rim staining. N = 159 total samples, *P < 0.05 compared to normal tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation. (C) Representative tissue microarray staining of emerin in 183 patients using emerin monoclonal antibodies (Leica, NCL-Emerin) or secondary alone (Vector Lab, cat#: MP-7452) using the same samples used in A. Nuclei are blue and emerin is brown. As aggressiveness of cases increases, there is a visible reduction in emerin expression and more deformed nuclei are present. (D) Quantification of emerin staining using the 0 to 3 grading system. N = 183 total samples #P < 0.02 compared to all non-cancerous tissue, *P < 0.0062 compared to both normal and benign tissue, one-way ANOVA and Dunnett’s test. Error bars represent standard deviation.

    Article Snippet: Reduced emerin expression at the nuclear periphery correlates with breast cancer invasiveness in patients. (A) Representative tissue microarray staining of emerin in 159 patients using emerin polyclonal antibodies (Proteintech, cat# 10351-1-AP) or secondary alone (Vector Lab, cat#: MP-7451).

    Techniques: Expressing, Microarray, Staining, Plasmid Preparation, Standard Deviation, Bioprocessing