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lentivirus vector  (ATCC)


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    Structured Review

    ATCC lentivirus vector
    Lentivirus Vector, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+atcc/Lentivirus+vector%3Breference+material/pmc12283003-303-7-21
    Average 93 stars, based on 10 article reviews
    lentivirus vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation.
    Article Snippet: .. The lentivirus vector was produced by tripartite-transfection of pCMV-VSV-G-RSV-Rev, pCAG-HIVgp and pCW57.1KSHVK-Rta/ORF50 into 293 T cells (ATCC CRL-3216)59,60. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Treatment of cancer using chimeric CD3 receptor proteins
    Article Snippet: .. Lentivirus vector titers were determined using limited dilution on Sup-T1 cells (ATCC) cultured in RPMI-1640 supplemented with 10% FBS. .. Vectors were 3-fold serial diluted then 50 uL of diluted vector was added to a flat bottom microtiter plate containing Sup-T1 cells.

    Article Title: KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation
    Article Snippet: .. The lentivirus vector was produced by tripartite-transfection of pCMV-VSV-G-RSV-Rev, pCAG-HIVgp and pCW57.1-KSHVK-Rta/ORF50 into 293 T cells (ATCC CRL-3216) , . ..

    Article Title: Erythropoietin-Modified Mesenchymal Stem Cells Enhance Anti-fibrosis Efficacy in Mouse Liver Fibrosis Model
    Article Snippet: .. After the lentivirus vector was constructed, viral packaging was carried out in HEK 293T (ATCC, ACS-4500) cells. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Produced:

    Article Title: KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation.
    Article Snippet: .. The lentivirus vector was produced by tripartite-transfection of pCMV-VSV-G-RSV-Rev, pCAG-HIVgp and pCW57.1KSHVK-Rta/ORF50 into 293 T cells (ATCC CRL-3216)59,60. ..

    Article Title: KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation
    Article Snippet: .. The lentivirus vector was produced by tripartite-transfection of pCMV-VSV-G-RSV-Rev, pCAG-HIVgp and pCW57.1-KSHVK-Rta/ORF50 into 293 T cells (ATCC CRL-3216) , . ..

    Clone Assay:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Targeting Androgen Receptor to Suppress Macrophage-induced EMT and Benign Prostatic Hyperplasia (BPH) Development
    Article Snippet: .. To generate AR overexpressed stable clones of the BPH-1 cell line, the lentivirus vector, pWPI-AR, or pWPI-control was cotransfected with pMD2.G and pAX2 into 293T cells (ATCC). .. After transfection for 48 h, the cultured medium of 293T cells was harvested and mixed with fresh F-12K culture medium (ratio 1:1) and 8 μg/ml polybrene (Millipore Corp., Billerica, MA), and then incubated with BPH-1 cells for 24 h. The viral-infected BPH-1 cells contain green fluorescence protein (GFP), so the BPH-AR or BPH-vector stable cells were obtained by the flow cytometry sorting.

    Article Title: Targeting Androgen Receptor to Suppress Macrophage-induced EMT and Benign Prostatic Hyperplasia (BPH) Development
    Article Snippet: .. Plasmids and stable cell lines To generate AR overexpressed stable clones of the BPH-1 cell line, the lentivirus vector, pWPI-AR, or pWPI-control was cotransfected with pMD2.G and pAX2 into 293T cells (ATCC). .. After transfection for 48 h, the cultured medium of 293T cells was harvested and mixed with fresh F-12K culture medium (ratio 1:1) and 8 μg/ml polybrene (Millipore Corp., Billerica, MA), and then incubated with BPH-1 cells for 24 h. The viral-infected BPH-1 cells contain green fluorescence protein (GFP), so the BPH-AR or BPH-vector stable cells were obtained by the flow cytometry sorting.

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Recombinant:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Transfection:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Amplification:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Concentration Assay:

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease.
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Article Title: Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease
    Article Snippet: .. Briefly, the plasmid was cloned into the lentivirus vector, and then the recombinant lentivirus was further transfected into the HEK-293T cells (ATCC, CRL-3216) for amplification and concentration. ..

    Cell Culture:

    Article Title: Treatment of cancer using chimeric CD3 receptor proteins
    Article Snippet: .. Lentivirus vector titers were determined using limited dilution on Sup-T1 cells (ATCC) cultured in RPMI-1640 supplemented with 10% FBS. .. Vectors were 3-fold serial diluted then 50 uL of diluted vector was added to a flat bottom microtiter plate containing Sup-T1 cells.

    Construct:

    Article Title: Erythropoietin-Modified Mesenchymal Stem Cells Enhance Anti-fibrosis Efficacy in Mouse Liver Fibrosis Model
    Article Snippet: .. After the lentivirus vector was constructed, viral packaging was carried out in HEK 293T (ATCC, ACS-4500) cells. ..

    Stable Transfection:

    Article Title: Targeting Androgen Receptor to Suppress Macrophage-induced EMT and Benign Prostatic Hyperplasia (BPH) Development
    Article Snippet: .. Plasmids and stable cell lines To generate AR overexpressed stable clones of the BPH-1 cell line, the lentivirus vector, pWPI-AR, or pWPI-control was cotransfected with pMD2.G and pAX2 into 293T cells (ATCC). .. After transfection for 48 h, the cultured medium of 293T cells was harvested and mixed with fresh F-12K culture medium (ratio 1:1) and 8 μg/ml polybrene (Millipore Corp., Billerica, MA), and then incubated with BPH-1 cells for 24 h. The viral-infected BPH-1 cells contain green fluorescence protein (GFP), so the BPH-AR or BPH-vector stable cells were obtained by the flow cytometry sorting.



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    Image Search Results


    Improving the tolerance to formaldehyde via ALE. (A) Growth of FM-1 in minimal medium with 10 g/L glucose and different formaldehyde concentrations. 0 mM (square), 0.5 mM (triangle), 0.8 mM (circle), and 1 mM (inverted triangle). (B) ALE procedure of culture-1 in CGXII minimal medium supplemented with different formaldehyde concentrations and 10 g/L glucose. (C) Growth curve of the evolved mutants in CGXII minimal medium supplemented with 10 g/L glucose and 0.8 mM formaldehyde. (D) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose and 1.6 mM formaldehyde. (E) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose. (F) Formaldehyde degradation during cell growth of wild-type C. glutamicum ATCC 13032, FM-1 and FM-3. Values and error bars reflect the mean ± s.d. of three biological replicates (N = 3).

    Journal: Synthetic and Systems Biotechnology

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress

    doi: 10.1016/j.synbio.2026.01.020

    Figure Lengend Snippet: Improving the tolerance to formaldehyde via ALE. (A) Growth of FM-1 in minimal medium with 10 g/L glucose and different formaldehyde concentrations. 0 mM (square), 0.5 mM (triangle), 0.8 mM (circle), and 1 mM (inverted triangle). (B) ALE procedure of culture-1 in CGXII minimal medium supplemented with different formaldehyde concentrations and 10 g/L glucose. (C) Growth curve of the evolved mutants in CGXII minimal medium supplemented with 10 g/L glucose and 0.8 mM formaldehyde. (D) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose and 1.6 mM formaldehyde. (E) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose. (F) Formaldehyde degradation during cell growth of wild-type C. glutamicum ATCC 13032, FM-1 and FM-3. Values and error bars reflect the mean ± s.d. of three biological replicates (N = 3).

    Article Snippet: Each evolved strain possesses more than 100 mutations including single nucleotide polymorphisms (SNPs), insertions, and deletions, which were aligned against the C. glutamicum ATCC 13032 reference genome (GenBank accession number GCA_000011325.1).

    Techniques: Mutagenesis