ice bucket, rectangular (Fisher Scientific)

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Fisher Scientific ice bucket, rectangular
Ice Bucket, Rectangular, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rectangular+bucket/ice+bucket/pmc06922010-19-0-4
Average 90 stars, based on 1 article reviews

ice bucket, rectangular - by Bioz Stars, 2026-10

90/100 stars

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Article Title: An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
Article Snippet: QIAQuick PCR purification kit (QIAGEN, catalog number: 28106) iQ SYBR Green Supermix, 500 × 50 μI rxns (Bio-Rad Laboratories, catalog number: 1708882) Instant non-fat dry milk (Carnation) Antibody ( i.e. , α-TBP, monoclonal, Abcam, catalog number: ab61411) Secondary ECL-conjugated antibody (we use Amersham ECL mouse or rabbit IgG HRP-linked whole Ab, GE Healthcare, catalog numbers: NXA931 or NA934V) Amersham ECL Prime Western blotting detecting reagent (GE Healthcare, catalog number: RPN2232) Yeast extract (BD, BactoTM, catalog number: 212750) Bacto peptone (BD, BactoTM, catalog number: 211677) Sugar source, i.e. ,: D-(+)-Glucose (Sigma-Aldrich, catalog number: G7021) D-(+)-Galactose (Sigma-Aldrich, catalog number: G5388) D-(+)-Raffinose (MP Biomedicals, catalog number: 02102797) Difco yeast nitrogen base without amino acids and ammonium sulfate (BD, DifcoTM, catalog number: 233520) Note: Yeast nitrogen base without amino acids (with or without sugar source) can be used as an alternative and doesn’t require the addition of ammonium sulfate (i.e., Sigma-Aldrich, catalog number: Y0626) .

Article Title: An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
Article Snippet: Pipette tips Reusable cotton-plug top serological (glass) pipettes (can also use single-use plastic pipettes) 10 ml pipettes (Fisher Scientific, catalog number: 13-675M) 25 ml pipettes (Fisher Scientific, catalog number: 13-675N) Pipette sterilizing boxes (Fisher Scientific, catalog number: 03-465) Nalgene PPCO centrifuge bottles with sealing closure (Thermo Fisher Scientific, Thermo ScientificTM, catalog number: 3141-0500) 50 ml conical tubes 30 × 115 mm (Corning, Falcon ® , catalog number: 352070) 2.0 ml microcentrifuge conical screw cap tubes (FastPrep tubes, Fisher Scientific, catalog number: 02-681-344) Microcentrifuge tube screw caps with O-Rings (for FastPrep tubes, Fisher Scientific, catalog number: 02-681-366) Acid washed 425-600 μm glass beads (Sigma-Aldrich, catalog number: G8772) 18 gauge needle (PrecisionGlide, BD, catalog number: 305195) Disposable culture tubes, glass 13 × 100 mm (Fisher Scientific, catalog number: 14-961-27) 1.5 ml microcentrifuge tubes graduated (Fisher Scientific, catalog number: 05-408-129) Whatman filter paper (GE Healthcare, catalog number: 1003-917) Autoradiography film (Genesee Scientific, catalog number: 30-101) 1.5-1.7 ml polypropylene graduated tube with locking lid (Fisher Scientific, catalog number: 02-681-285) Hard-Shell High-Profile 96-well semi-skirted PCR plates (Bio-Rad Laboratories, catalog number: HSS9641) Tubular roll stock (to seal membrane in plastic before developing; Ampac, catalog number: TRS-95125-3) Immobilon-P membrane (PVDF; Merck, catalog number: IPVH00010) Microseal ‘B’ seal seals (Bio-Rad Laboratories, catalog number: MSB1001) Stericup sterile vacuum filter units, 500 ml (Merck, catalog number: SCGPU05RE) Yeast strains, wild type (WT) and overexpression (OE) for each TF of interest (see Poorey et al. , 2013 and Zaidi et al. , 2017 for strain lists) Plasmids for TF overexpression and vector control for WT strain (see Poorey et al. , 2013 and Zaidi et al. , 2017 for plasmid lists) Locus specific primers (Invitrogen) In-Fusion HD cloning kit (Takara Bio, Clontech, catalog number: 639649) Ice bucket 37% formaldehyde (Fisher Scientific, catalog number: F79) Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, catalog number: 500-0006) nProtein A Sepharose 4 Fast Flow beads (GE Healthcare, catalog number: 17-5280-01) Note: Ab-conjugated beads can be used instead, such as IgG Sepharose 6 Fast Flow (GE Healthcare, catalog number: 17-0969-01) and Sepharose 6 Fast Flow beads (GE Healthcare, catalog number: 17-0159-99) .

Article Title: Oxidative Stress, DNA Damage, and Antioxidant Enzyme Activity Induced by Hexavalent Chromium in Sprague-Dawley Rats
Article Snippet: This was vortexed well and kept on ice (Ice Bucket, Fischer-Scientific, Pittsburg, PA, USA) for 30 min. Then, the tubes containing the sample and the blank were brought to room temperature and 0.01 mL of Triton X-100 was added and vortexed well till the whole tissue extract was completely dissolved.

Article Title: Circulating biomarkers for metastatic prostate cancer
Article Snippet: Ice bucket, Fisher, catalog number 02-591-46 (Fisher Scientific, Part of Thermo Fisher Scientific, Pittsburgh, Pa.) j.

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Article Title: An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Article Snippet: Avant 1.7 ml microcentrifuge tubes (MIDSCI, catalog number: AVSS1700) Pipette tips, 20 μl (Rainin Instrument, LLC, catalog number: 17005091) Pipette tips, 250 μl (Rainin Instrument, LLC, catalog number: 17005093) Razor blades (Fisher Scientific, catalog number: 12640) Microcentrifuge tube rack (Fisher Scientific, catalog number: 22–313630) Scotch double-sided tape, 3⁄4 in. x 400 in. (3M) Microscope slides, 3 in. x 1 in. (Fisher Scientific, catalog number: 12518100A3) Cover glass, No. 1.5, 30 mm x 22 mm (Fisher Scientific, catalog number: 12544A) Filter forcep (EMD Millipore, catalog number: XX6200006P) Kimwipe (Fisher Scientific, catalog number: 06666A) Microscope lens paper, 4 in. x 6 in. sheets (Fisher Scientific, catalog number: 11996) Unlabeled porcine tubulin, > 99% pure, 1 mg (Cytoskeleton, Inc., catalog number: T240) (store at −80 °C) HiLyte Fluor 488 labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL488M) (store at −80 °C) Rhodamine labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL590M) (store at −80 °C) 100 mM GTP (Cytoskeleton, Inc., catalog number: BST06–010) (store at −20 °C) 10 mM Taxol (Cytoskeleton, Inc., catalog number: TXD01) (store at −20 °C) Precision Red Advanced Protein Assay Reagent (Cytoskeleton, Inc., catalog number: ADV02) (store at room temperature) PIPES (Sigma-Aldrich, catalog number: P1851) (store at room temperature) EGTA (Fisher Scientific, catalog number: O2783–100) (store at room temperature) Magnesium Chloride Hexahydrate (Fisher Scientific, catalog number: BP214–500) Ultrapure water from Milli-Q ® Advantage A10 Water Purification System (Millipore Sigma) THETM alpha Tubulin Antibody, mAb, mouse (GenScript, catalog number: A01410) (store at −20 °C) Pluronic F-127, 100 g (Anatrace, catalog number: P305) (store at room temperature) Carl Zeiss ImmersolTM 518 F Immersion Oil (Fisher Scientific, catalog number: 1262466A) (store at room temperature) PEM Buffer (see Recipes ) Blocking buffer (see Recipes ) .. Ice bucket (Fisher Scientific, catalog number: 07210123) Milli-Q ® Advantage A10 Water Purification System (Millipore Sigma) Sorvall centrifuge equipped with S120-AT2 rotor (Thermo Fisher Scientific, model: Discovery) 37 °C incubator (Fisher Scientific, catalog number: 11690525D) Microcentrifuge (Eppendorf, model: 5424) Inverted fluorescence microscope equipped with Perfect Focus System and a 100×/1.49 NA objective (Nikon, model: Ti-E) Laser launch system with 405/488/561/640 nm wavelengths at 15 mW per line (Nikon, model: LUN4) Electron multiplying CCD camera (Andor Technology, model: iXon 888) GFP filter cube set for imaging HiLyte Fluor 488 or Alexa Fluor 488 fluorescence (Chroma Technology Corp., catalog number: 49002) TRITC filter cube set for imaging rhodamine or TMR fluorescence (Chroma Technology Corp., catalog number: 49008) HP workstation with 3.5 GHz 6 core Xeon processor, 64 GB memory, 1 TB hard drive, and 64bit Windows 7 (Micro Video Instruments, Inc., catalog number: 99909) Pipet-Lite LTS pipette L-20XLS+ (Rainin Instrument, LLC, catalog number: 17014392) Pipet-Lite LTS pipette L-200XLS+ (Rainin Instrument, LLC, catalog number: 17014391) .. NIS-Elements Advanced Research (Nikon) ( https://www.nikoninstruments.com/Products/Software/NIS-Elements-Advanced-Research ) ImageJ (NIH, https://imagej.nih.gov/ij/download.html ) KaleidaGraph ( http://www.synergy.com/wordpress_650164087/kaleidagraph/ )

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Article Title: Simple Workflow and Comparison of Media for hPSC-Cardiomyocyte Cryopreservation and Recovery.
Article Snippet: .. Materials Geltrex (Thermo Fisher, cat. no. A1413302) DMEM/F-12 (Thermo Fisher, cat. no. 11330032) CM Suspension medium (see recipe) Dry ice or liquid nitrogen Cryovials of cryopreserved hPSC-CMs (see Basic Protocol 1) RevitaCell TM Supplement 100× (Thermo Fisher, cat. no. A2644501) CM Maintenance medium (see recipe) Trypan blue solution (e.g., Sigma Aldrich, cat. no. T8154) Multi-well culture plates (e.g., 24-well, Greiner Bio-One, cat. no. 662160) Parafilm® M sealing film (Sigma, cat. no. P6543-1EA) Isothermal ice bucket (e.g., Fisherbrand TM Polyurethane Ice Buckets, Fisher Scientific, cat. no. 11324085) Water bath (e.g., VWR, cat. no. 462-0554) 1000-μl manual pipette plus 1000-μl filter tips (e.g., Eppendorf, cat. no. 613-0866; Biozym, cat. no. VT0270) 15- and 50-ml Falcon TM polypropylene tubes (e.g., Corning, cat. nos. .. 352096 and 352070) Pipette gun and 5-10-ml stripettes (e.g., INTEGRA Biosciences, cat. no. 156400; Sarstedt, 86.1253.001; Sarstedt86.1254.001) Centrifuge (e.g., Eppendorf 5810R, Thermo Fisher, cat. no. 10012724) 37°C, 5% CO2 incubator Countess TM II automated cell counter (Thermo Fisher, cat. no. AMQAX1000) Countess TM cell counting slides (Thermo Fisher, cat. no. C10228) Miller et al. 9 of 18 Current Protocols in Stem Cell Biology Table 3 Volumes of CM Suspension Medium Required for the Resuspension of hPSC-CMs Immediately After Thawinga No. cryovials Volume frozen cells Volume initial resuspension Top up volume 1 0.5-1 ml 1 ml 5 ml 2 1-2 ml 2 ml 8 ml aThe volume of initial resuspension is to be added dropwise using a 1000 pipette-μl, with the top up volume subsequently added dropwise using a 5-10-ml stripette.

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