gene exp hsf1 hs01027608 g1 (Thermo Fisher)
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gene exp hsf1 hs01027608 g1
Gene Exp Hsf1 Hs01027608 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/real-time+executable+program+in+c+++code/pmc03953446-61-31-13?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
Gene Exp Hsf1 Hs01027608 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/real-time+executable+program+in+c+++code/pmc03953446-61-31-13?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp hsf1 hs01027608 g1 - by Bioz Stars,
2026-08
85/100 stars
Images
1) Product Images from "Transcriptional and Epigenetic Regulation of KIF14 Overexpression in Ovarian Cancer"
Article Title: Transcriptional and Epigenetic Regulation of KIF14 Overexpression in Ovarian Cancer
Journal: PLoS ONE
doi: 10.1371/journal.pone.0091540
Figure Legend Snippet: Promoter deletion constructs fused to Luciferase were tested for activity in HeLa, SKOV3 and OvCa429 cells. pRSV-Luc, positive control, 0, empty vector control. Numbers are base pairs relative to the transcriptional start site (0). Bioinformatic analysis (Genomatix) of the most active region within the KIF14 promoter (−2366 to −2245) identified putative binding sites for transcription factors YY1, HSF1 and Sp1. Specific transcription factor recognition sites are underlined, and space within sequence denotes location of deletion constructs. N = 3, * Significance at P <0.05, unpaired t-test. P = 0.01 (HeLa); P = 0.01 (SKOV3); P = 0.03 (OvCa429).
Techniques Used: Construct, Luciferase, Activity Assay, Positive Control, Plasmid Preparation, Control, Binding Assay, Sequencing
Figure Legend Snippet: siRNA knockdown of endogenous Sp1 (orange), HSF1 (green), and YY1 (red) transcription factors, and measurement of their mRNA expression along with corresponding KIF14 mRNA levels (blue) via real-time PCR in A SKOV3 and B OvCa429 cells. Y-axes: normalized mRNA expression relative to MOCK. GL2, control siRNA; N = 3, * Significance at P <0.05, unpaired t-test. Three different siRNA molecules (A, B, C) were used to knock down each gene. P values for panel A (SKOV3 cells): P = 0.02 for Sp1 expression (orange) with Sp1 siRNAs A, B, and C; P = 0.03 (siRNA-A), P = 0.047 (siRNA–B), P = 0.04 (siRNA–C) for KIF14 expression (blue). P = 0.001 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.23 (siRNA-A), P = 0.12 (siRNA-B), P = 0.4 (siRNA-C) for KIF14 expression (blue). P = 0.01 for YY1 expression (red) with YY1 siRNAs A, B and C; P = 0.006 for KIF14 expression (blue) with YY1 siRNAs A, B, and C. P values for panel B (OvCa429 cells): P = 0.03 for Sp1 expression (orange) with Sp1 siRNAs A, B, and C; P = 0.05 (siRNA-A), P = 0.04 (siRNA-B), P = 0.045 (siRNA-C) for KIF14 expression (blue). P = 0.003 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.31 (siRNA-A), P = 0.45 (siRNA-B), P = 0.39 (siRNA-C) for KIF14 expression (blue). P = 0.02 (siRNA-A), P = 0.01 (siRNA-B), P = 0.01 for YY1 expression (red); P = 0.02 (siRNA-A), P = 0.001 (siRNA-B), P = 0.006 (siRNA-C) for KIF14 expression (blue).
Techniques Used: Knockdown, Expressing, Real-time Polymerase Chain Reaction, Control
Figure Legend Snippet: A siRNA knockdown of endogenous Sp1 (orange), HSF1 (green), and YY1 (red) transcription factors, and measurement of their protein expression along with corresponding KIF14 protein levels (blue) via immunoblot in SKOV3 cells. x-axis: normalized protein expression relative to MOCK. B Representative immunoblot of KIF14 and transcription factor expression. Numbers represent normalized expression values for the experiment shown. Similar results were seen with OvCa429 cells. GL2, control siRNA; N = 3; *, P <0.05 for transcription factor expression; #, P <0.05 for KIF14 expression, unpaired t-test. P values for panel A (SKOV3 cells): P = 0.009 (siRNA-A), P = 0.003 (siRNA-B), P = 0.006 (siRNA-C) for Sp1 expression (orange); P = 0.005 (siRNA-A), P = 0.007 (siRNA-B), P = 0.004 (siRNA-C) for KIF14 expression (blue). P = 0.01 for HSF1 expression (green) with HSF1 siRNAs A, B, and C; P = 0.54 (siRNA-A), P = 0.65 (siRNA-B), P = 0.41 (siRNA-C) for KIF14 expression (blue). P = 0.001 for YY1 expression (red) with YY1 siRNAs A, B and C; P = 0.01 (siRNA-A), P = 0.02 (siRNA-B), P = 0.005 (siRNA-C) for KIF14 expression (blue).
Techniques Used: Knockdown, Expressing, Western Blot, Control
Figure Legend Snippet: ChIP assays of endogenous YY1, Sp1 and HSF1 followed by real time PCR with the KIF14 promoter region (−2300 to −2133) in cell lines SKOV3, OvCa429, and HeLa compared to IgG (negative control). Values represent average quantity of promoter region product relative to IgG control. Error bars represent standard deviation of triplicate assays.
Techniques Used: Real-time Polymerase Chain Reaction, Negative Control, Control, Standard Deviation
Figure Legend Snippet: Quantitative mRNA expression analysis of primary OvCa tumors with KIF14 HIGH (red) and KIF14 LOW (green) mRNA expression (but no KIF14 genomic gain) for Sp1 (circle), YY1 (triangle), and HSF1 (diamond) normalized to normal ovary expression (set as 1, black dashed line). Mean, black line. Individual tumors represented by symbols. * Significance at P <0.05, paired t-test. P = 0.03 (Sp1), P = 0.01 (YY1), P = 0.32 (HSF1).
Techniques Used: Expressing
Figure Legend Snippet: Pearson correlation between KIF14 gain/no gain tumors and Sp1 , HSF1 and YY1 mRNA.
Techniques Used: