addgene 208775 (Addgene inc)
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addgene 208775
Addgene 208775, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/randomization/pCMV-luc-random+(Plasmid+%2320877)/pmc12796307-16-9-9
Average 93 stars, based on 4 article reviews
Addgene 208775, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/randomization/pCMV-luc-random+(Plasmid+%2320877)/pmc12796307-16-9-9
Average 93 stars, based on 4 article reviews
addgene 208775 - by Bioz Stars,
2026-10
93/100 stars
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Plasmid Preparation:Article Title: NINJ1 is a restriction factor for HSV-1 in mouse macrophages Article Snippet: Primary antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (4030-05; Southern Biotech; 1:5000), HRP-conjugated goat anti-mouse (1034-05; Southern Biotech; 1:5000 or 12-349; MilliporeSigma; 1:2000) secondary antibodies. .. Article Title: NINJ1 induces plasma membrane rupture and release of damage-associated molecular pattern molecules during ferroptosis Article Snippet: Doxycycline inducible C-terminal GFP tagged hNINJ1 (hNINJ1-GFP) is Addgene plasmid 208779 and was generated as previously described (Degen et al, ). .. Plasmids for the MSCV experiments are as follows: MSCV-IRES-GFP is available on Addgene (20672); MSCV-WT mouse NINJ1-GFP (Addgene plasmid 208776); MSCV-K45Q-NINJ1-GFP (Addgene plasmid 208777) and Article Title: NINJ1 blocks HSV-1 entry into macrophages to impact viral replication and immunity Article Snippet: Primary antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (4030-05; Southern Biotech; 1:5000), HRP-conjugated goat anti-mouse (1034-05; Southern Biotech; 1:5000 or 12-349; MilliporeSigma; 1:2000) secondary antibodies. .. Article Title: NINJ1 blocks HSV-1 entry into macrophages to impact viral replication and immunity. Article Snippet: Primary antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (4030-05; Southern Biotech; 1:5000), HRP-conjugated goat anti-mouse (1034-05; Southern Biotech; 1:5000 or 12-349; MilliporeSigma; 1:2000) secondary antibodies. .. Article Title: NINJ1 induces plasma membrane rupture and release of damage-associated molecular pattern molecules during ferroptosis Article Snippet: The plasmids used in this manuscript are all available on Addgene. .. Doxycycline inducible C-terminal Transfection:Article Title: NINJ1 is a restriction factor for HSV-1 in mouse macrophages Article Snippet: Primary antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (4030-05; Southern Biotech; 1:5000), HRP-conjugated goat anti-mouse (1034-05; Southern Biotech; 1:5000 or 12-349; MilliporeSigma; 1:2000) secondary antibodies. .. Article Title: NINJ1 blocks HSV-1 entry into macrophages to impact viral replication and immunity Article Snippet: Primary antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (4030-05; Southern Biotech; 1:5000), HRP-conjugated goat anti-mouse (1034-05; Southern Biotech; 1:5000 or 12-349; MilliporeSigma; 1:2000) secondary antibodies. .. Article Title: NINJ1 blocks HSV-1 entry into macrophages to impact viral replication and immunity. Article Snippet: Primary antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (4030-05; Southern Biotech; 1:5000), HRP-conjugated goat anti-mouse (1034-05; Southern Biotech; 1:5000 or 12-349; MilliporeSigma; 1:2000) secondary antibodies. .. Generated:Article Title: NINJ1 induces plasma membrane rupture and release of damage-associated molecular pattern molecules during ferroptosis Article Snippet: Doxycycline inducible C-terminal GFP tagged hNINJ1 (hNINJ1-GFP) is Addgene plasmid 208779 and was generated as previously described (Degen et al, ). .. Plasmids for the MSCV experiments are as follows: MSCV-IRES-GFP is available on Addgene (20672); MSCV-WT mouse NINJ1-GFP (Addgene plasmid 208776); MSCV-K45Q-NINJ1-GFP (Addgene plasmid 208777) and Article Title: NINJ1 induces plasma membrane rupture and release of damage-associated molecular pattern molecules during ferroptosis Article Snippet: The plasmids used in this manuscript are all available on Addgene. .. Doxycycline inducible C-terminal Recombinant:Article Title: NINJ1 blocks HSV-1 entry into macrophages to impact viral replication and immunity. Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK 293T cells (H. sapiens) ATCC CRL 3216 Vero cells (C. aethiops) Florian Schmidt (Uni Bonn) N/A BSC-40 cells (C. aethiops) Florian Schmidt (Uni Bonn) N/A Wildtype BMDM (M. musculus) This study N/A Ninj1−/− BMDM (M. musculus) This study N/A Wildtype iBMDM (M. musculus) This study N/A Ninj1−/− iBMDM (M. musculus) This study N/A MEF (M. musculus) Dondelinger et al, (2023) N/A Ninj1 ko MEF (M. musculus) Dondelinger et al, (2023) N/A 3T3 (M. musculus) Ramos et al, (2024) N/A Ninj1 ko 3T3 (M. musculus) Ramos et al, (2024) N/A Reagent/resource Reference or source Identifier or catalog number L929 (M. musculus) ATCC NCTN Clone 929 KOS HSV-1 ICP47 GFP Florian Schmidt (Uni Bonn) N/A VACVWestern Reserve EL EGFP Florian Schmidt (Uni Bonn) N/A Recombinant DNA pLVX mNINJ1 Ramos et al, (2024) Sequencing:Article Title: NINJ1 blocks HSV-1 entry into macrophages to impact viral replication and immunity. Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK 293T cells (H. sapiens) ATCC CRL 3216 Vero cells (C. aethiops) Florian Schmidt (Uni Bonn) N/A BSC-40 cells (C. aethiops) Florian Schmidt (Uni Bonn) N/A Wildtype BMDM (M. musculus) This study N/A Ninj1−/− BMDM (M. musculus) This study N/A Wildtype iBMDM (M. musculus) This study N/A Ninj1−/− iBMDM (M. musculus) This study N/A MEF (M. musculus) Dondelinger et al, (2023) N/A Ninj1 ko MEF (M. musculus) Dondelinger et al, (2023) N/A 3T3 (M. musculus) Ramos et al, (2024) N/A Ninj1 ko 3T3 (M. musculus) Ramos et al, (2024) N/A Reagent/resource Reference or source Identifier or catalog number L929 (M. musculus) ATCC NCTN Clone 929 KOS HSV-1 ICP47 GFP Florian Schmidt (Uni Bonn) N/A VACVWestern Reserve EL EGFP Florian Schmidt (Uni Bonn) N/A Recombinant DNA pLVX mNINJ1 Ramos et al, (2024) |