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random n6 primers (New England Biolabs)

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New England Biolabs random n6 primers
Random N6 Primers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/randomization/Random+Primer+6/pmc08533754-95-40-44
Average 95 stars, based on 448 article reviews

random n6 primers - by Bioz Stars, 2026-11

95/100 stars

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Article Title: DdmABC-dependent death triggered by viral palindromic DNA sequences
Article Snippet: Briefly, chromosomal and plasmid DNA was separated on a 0.7% agarose gel and then denatured, neutralized, and transferred to a BrightStar-Plus Positively Charged Nylon Membrane (Invitrogen AM10100) in 10X SSC buffer.

Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA.
Article Snippet: cDNAs were size selected twice using NEBNext DNA Sample Purification Beads (NEB, E6178) by incubating with 0.97% PEG8000 and 97 mM MgCl 2 for 30 minutes ( 26 ).

Incubation:

Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA
Article Snippet: Remaining RNAs were treated with 200 units of RNase R (Lucigen, RNR07250) at 37°C for 30 min, then purified with NEBNext RNA Sample Purification Beads (NEB, E6351). .. 4 μg of total RNA from human brain (BioChain, Catalog #: R1234035-P, Lot #: C404081), HEK293 cells or lung tumor (BioChain, Catalog #: R1235152-50, Lot #: A808151) or ribo-depleted and RNaseR treated RNA (500–700 ng) from human brain and HEK293 cells were incubated with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 10 μl at 72°C for 5 minutes then cooled down to 4°C with 0.1°C/s ramp. .. Then 2 μl 10mM dNTPs and 400 units of Induro RT (NEB, M0681) were added to a final volume of 40 μl in the 1× Induro-RT buffer supplemented with 3 mM MgCl 2 .

Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA
Article Snippet: .. For human plasma samples (AMSBIO, 100048013 and 100028013), around 10 ng of extracted, DNaseI treated RNA in 10 μl was mixed with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 12 μl and incubated at 72°C for 5 minutes then directly cooled down to 4°C. .. For human plasma samples (AMSBIO, 100048013 and 100028013), around 10 ng of extracted, DNaseI treated RNA in 10 μl was mixed with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 12 μl and incubated at 72°C for 5 minutes then directly cooled down to 4°C.

Clinical Proteomics:

Article Title: Induro-RT mediated circRNA-sequencing (IMCR-seq) enables comprehensive profiling of full-length and long circular RNAs from low input total RNA
Article Snippet: .. For human plasma samples (AMSBIO, 100048013 and 100028013), around 10 ng of extracted, DNaseI treated RNA in 10 μl was mixed with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 12 μl and incubated at 72°C for 5 minutes then directly cooled down to 4°C. .. For human plasma samples (AMSBIO, 100048013 and 100028013), around 10 ng of extracted, DNaseI treated RNA in 10 μl was mixed with 2 μl from 100 μM random N6 primers (NEB, S1230S) in a total volume of 12 μl and incubated at 72°C for 5 minutes then directly cooled down to 4°C.

cDNA Synthesis:

Article Title: Direct genome sequencing of respiratory viruses from low viral load clinical specimens using the target capture sequencing technology.
Article Snippet: Libraries for the target capture sequencing were prepared using a Twist Comprehen sive Viral Research Panel (Twist Bioscience) as follows. .. Fifteen microliters of each RNA was converted to cDNA using Protoscript II First-Strand cDNA synthesis (NEB) and random primer 6 (NEB). ..

Article Title: Direct genome sequencing of respiratory viruses from low viral load clinical specimens using the target capture sequencing technology
Article Snippet: Libraries for the target capture sequencing were prepared using a Twist Comprehensive Viral Research Panel (Twist Bioscience) as follows. .. Fifteen microliters of each RNA was converted to cDNA using Protoscript II First-Strand cDNA synthesis (NEB) and random primer 6 (NEB). ..