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anti fh rabbit polyclonal  (Proteintech)


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    Structured Review

    Proteintech anti fh rabbit polyclonal
    Anti Fh Rabbit Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+complement/Complement+factor+H+Antibody/pm41781748-285-17-20
    Average 88 stars, based on 4 article reviews
    anti fh rabbit polyclonal - by Bioz Stars, 2026-11
    88/100 stars

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    Related Articles

    Staining:

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: .. Cells were stained with 1:50 α-p53 mAb (SantaCruz sc126) or anti-FH OX24 (Mouse, expressed in house) or anti-FH rabbit polyclonal (Proteintech, ref 12748-1-AP) or isotype controls (Dako FLEX negative control mouse and rabbit cocktail, catalog no IR750 and IR600 respectively) overnight at 4°C, and then with Phalloidin-IFluor 488 reagent (Abcam ab176753) for 90 minutes in the dark. .. were used 70,71 at different concentrations ranging from 500 nM to 15.62 nM or 250 μM to 7.8 nM diluted in either normal or low ionic strength buffers.Data were analyzed using BIAevaluation software (version 4.1; Biacore, GE Healthcare) and the binding to the empty chip sensor surface was subtracted to estimate the kinetic binding constants.

    Negative Control:

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: .. Cells were stained with 1:50 α-p53 mAb (SantaCruz sc126) or anti-FH OX24 (Mouse, expressed in house) or anti-FH rabbit polyclonal (Proteintech, ref 12748-1-AP) or isotype controls (Dako FLEX negative control mouse and rabbit cocktail, catalog no IR750 and IR600 respectively) overnight at 4°C, and then with Phalloidin-IFluor 488 reagent (Abcam ab176753) for 90 minutes in the dark. .. were used 70,71 at different concentrations ranging from 500 nM to 15.62 nM or 250 μM to 7.8 nM diluted in either normal or low ionic strength buffers.Data were analyzed using BIAevaluation software (version 4.1; Biacore, GE Healthcare) and the binding to the empty chip sensor surface was subtracted to estimate the kinetic binding constants.

    Flow Cytometry:

    Article Title: In Vitro Characterization of Derrone as an Aurora Kinase Inhibitor.
    Article Snippet: .. Flow cytometry and xCelligence system were performed in the Key Laboratory of Enzyme and Protein Technology facilities (HUS). ..

    Vaccines:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Control:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Membrane:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Binding Assay:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Recombinant:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Article Title: Multiplex colon cancer marker panel
    Article Snippet: Detection antibody enzyme Secondary antibody Substrate 11 Rabbit anti-human Anti-Rabbit IgG HRP HRP SF3B3 pAb4) (Invitrogen) chromogene 0.7 mg/ml 1/50000 12 Rabbit anti-human ALP Colorimetric GARS pAb4) ALP 0.4 mg/ml substrate 13 Rabbit anti-human Anti-Rabbit IgG HRP HRP ENO1 pAb (Invitrogen) chromogene (Proteintech Group) 1/50000 0.3 mg/ml 14 Mouse anti-human Anti-Mouse IgG HRP HRP RCN1 mAb (Abnova) (Invitrogen) chromogene 0.5 mg/ml 1/10000 15 TABLE 4 Gene No. .. Protein Name Name ELISA Kit Standard protein Capture antibody 16 Protein S100- S100A8/A9 Calprotectin, Human, A8/8100-S9 ELISA kit (Hycult biotechnology, Plymouth Meeting, PA) 17 Heat shock protein HSPB1 Hsp27 ELISA Kit beta-1 (Calbiochem, Darmstadt, Germany) 18 Receptor-type PTPRA Recombinant PTPRA Rabbit anti-human tyrosine-protein (SignalChem, British PTPRA pAb phosphatase alpha Columbia, Canada) (Proteintech Group) 3 mg/ml 19 Endoplasmin HSP90B1 Recombinant TRA1 Mouse anti-human (Abnova) TRA1 mAb (Proteintech Group) 5 mg/ml 20 Complement factor H CFH Native Factor H Sheep anti-human (AbD serotec, Factor H pAb (Gene Kidlington, UK) Tex, Irvine, CA) 5 μg/ml Conjugated No. .. Detection antibody enzyme Secondary antibody Substrate 16 17 18 Rabbit anti-human ALP Chemilumine PTPRA pAb4) scent ALP 1 mg/ml substrate 19 Rabbit anti-human Anti-Rabbit IgG ALP Chemilumine TRA1 pAb (Invitrogen) scent ALP (Proteintech Group) 1/20000 substrate 0.5 mg/ml 20 Sheep anti-human ALP Colorimetric Factor H pAb (Gene ALP Tex) substrate 0.5 μg/ml TABLE 5 Gene No.

    Knockdown:

    Article Title: Four and a half LIM domains 1 (FHL1) and receptor interacting protein of 140kDa (RIP140) interact and cooperate in estrogen signaling.
    Article Snippet: Four and a half LIM domains 1 (FHL1) belongs to a family of LIM-only proteins that regulate gene transcription, cell proliferation, differentiation and apoptosis.. However, the biological function of FHL1 remains largely unknown.. We used a yeast two-hybrid system and identified receptor interacting protein of 140 kDa (RIP140) as a novel FHL1-binding protein.

    Western Blot:

    Article Title: Four and a half LIM domains 1 (FHL1) and receptor interacting protein of 140kDa (RIP140) interact and cooperate in estrogen signaling.
    Article Snippet: Four and a half LIM domains 1 (FHL1) belongs to a family of LIM-only proteins that regulate gene transcription, cell proliferation, differentiation and apoptosis.. However, the biological function of FHL1 remains largely unknown.. We used a yeast two-hybrid system and identified receptor interacting protein of 140 kDa (RIP140) as a novel FHL1-binding protein.

    other:

    Article Title: Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter
    Article Snippet: FHL1 (rabbit) antibody was purchased from ProteinTech.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Multiplex colon cancer marker panel
    Article Snippet: Detection antibody enzyme Secondary antibody Substrate 11 Rabbit anti-human Anti-Rabbit IgG HRP HRP SF3B3 pAb4) (Invitrogen) chromogene 0.7 mg/ml 1/50000 12 Rabbit anti-human ALP Colorimetric GARS pAb4) ALP 0.4 mg/ml substrate 13 Rabbit anti-human Anti-Rabbit IgG HRP HRP ENO1 pAb (Invitrogen) chromogene (Proteintech Group) 1/50000 0.3 mg/ml 14 Mouse anti-human Anti-Mouse IgG HRP HRP RCN1 mAb (Abnova) (Invitrogen) chromogene 0.5 mg/ml 1/10000 15 TABLE 4 Gene No. .. Protein Name Name ELISA Kit Standard protein Capture antibody 16 Protein S100- S100A8/A9 Calprotectin, Human, A8/8100-S9 ELISA kit (Hycult biotechnology, Plymouth Meeting, PA) 17 Heat shock protein HSPB1 Hsp27 ELISA Kit beta-1 (Calbiochem, Darmstadt, Germany) 18 Receptor-type PTPRA Recombinant PTPRA Rabbit anti-human tyrosine-protein (SignalChem, British PTPRA pAb phosphatase alpha Columbia, Canada) (Proteintech Group) 3 mg/ml 19 Endoplasmin HSP90B1 Recombinant TRA1 Mouse anti-human (Abnova) TRA1 mAb (Proteintech Group) 5 mg/ml 20 Complement factor H CFH Native Factor H Sheep anti-human (AbD serotec, Factor H pAb (Gene Kidlington, UK) Tex, Irvine, CA) 5 μg/ml Conjugated No. .. Detection antibody enzyme Secondary antibody Substrate 16 17 18 Rabbit anti-human ALP Chemilumine PTPRA pAb4) scent ALP 1 mg/ml substrate 19 Rabbit anti-human Anti-Rabbit IgG ALP Chemilumine TRA1 pAb (Invitrogen) scent ALP (Proteintech Group) 1/20000 substrate 0.5 mg/ml 20 Sheep anti-human ALP Colorimetric Factor H pAb (Gene ALP Tex) substrate 0.5 μg/ml TABLE 5 Gene No.



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    Cedarlane baby rabbit complement
    (A) Biofilm formation ability of commensal vs. inflammatory S. epidermidis strains. Biofilm positive strains were defined relative to a canonical biofilm-forming strain (Sep_1457). Numbers indicate strain counts per category and P-value and odds ratio calculated using Fisher’s exact test. (B-E) Survival ability in blood as measured by CFUs at 0, 2, and 24h of S. epidermidis strains (D) with respect to a bloodstream infection S. aureus strain (USA300LAC, (B)) with or without <t>complement</t> (+/-Comp) depletion (C, E). Points represent individual strains (or replicates for S. aureus) and boxes indicate IQR with median. Lines connect matched conditions. Significance was assessed by paired t-test with Bonferroni correction. (F-G) PCA plot of Staphylococcus cellular (F) or extracellular (G) metabolomes. Staphylococcus metabolomes were inferred by metabolic feature intensity within untargeted metabolomes. (H) Diagram of arginine metabolism pathway highlighting reactions catalyzed by arcA , argF , and argG . (I-K) Violin plots showing the metabolite abundance of arginine (I), carbamoyl-P (J), and argininosuccinate (K) in strains with or without arcA , argF , or argG. Points indicate normalized metabolite intensity; violins show distributions with embedded boxplots (median and IQR). Gene labels are black if acting on or downstream of the metabolite, grey otherwise. (L-N) Growth dynamics of inflammatory vs. commensal S. epidermidis strains in skin-like media with (+Arg) or without (-Arg) arginine. Maximum growth rate (L), maximum density (M), and area under the curve (AUC) (N) are derived from OD600 measurements every 0.5h over 10 hours at 37C. Points represent strains with paired conditions connected with lines. Statistical significance was determined by paired t -test followed by Bonferroni correction. (O-Q) Protein expression of cytokines IL-8 (O), CCL20 (P) and IL-36γ (Q) in the basal media of Staphylococcus -colonized RHE was measured by ELISA (n=3-4 replicates per strain in an independent experiment colonizing 3 inflammatory and 4 commensal S. epidermidis strains and S. aureus as a reference). Points represent individual replicates colored by cluster and boxes show IQR with median. (R) Model summarizing species- and strain-level effects of Staphylococcus on RHE. Species-level differences distinguish commensal and pathogenic effects, while strain-level variation within S. epidermidis identifies inflammatory strains that activate a proinflammatory transcriptional program including upstream regulators (e.g. NFKB), chemokines (e.g., CCL20), cytokines (e.g., IL36γ) and downstream antimicrobial effectors (e.g. LCN2). Inflammatory S. epidermidis also enhances skin barrier defensive genes, particularly members of the LCE and SPRR gene families, which contribute to both barrier integrity and antimicrobial defense, and are regulated by transcription factors such as MAFB. These effects are linked to differences in gene content (e.g., cell surface genes), transcriptional programs, metabolism (e.g., arginine pathway), and pathogen-like growth phenotypes. P-values: **** p ≤ 0.0001, *** 0.0001 < p ≤ 0.001, ** 0.001 < p ≤ 0.01, * 0.01 < p ≤ 0.05., ns p > 0.05.
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    (A) Biofilm formation ability of commensal vs. inflammatory S. epidermidis strains. Biofilm positive strains were defined relative to a canonical biofilm-forming strain (Sep_1457). Numbers indicate strain counts per category and P-value and odds ratio calculated using Fisher’s exact test. (B-E) Survival ability in blood as measured by CFUs at 0, 2, and 24h of S. epidermidis strains (D) with respect to a bloodstream infection S. aureus strain (USA300LAC, (B)) with or without complement (+/-Comp) depletion (C, E). Points represent individual strains (or replicates for S. aureus) and boxes indicate IQR with median. Lines connect matched conditions. Significance was assessed by paired t-test with Bonferroni correction. (F-G) PCA plot of Staphylococcus cellular (F) or extracellular (G) metabolomes. Staphylococcus metabolomes were inferred by metabolic feature intensity within untargeted metabolomes. (H) Diagram of arginine metabolism pathway highlighting reactions catalyzed by arcA , argF , and argG . (I-K) Violin plots showing the metabolite abundance of arginine (I), carbamoyl-P (J), and argininosuccinate (K) in strains with or without arcA , argF , or argG. Points indicate normalized metabolite intensity; violins show distributions with embedded boxplots (median and IQR). Gene labels are black if acting on or downstream of the metabolite, grey otherwise. (L-N) Growth dynamics of inflammatory vs. commensal S. epidermidis strains in skin-like media with (+Arg) or without (-Arg) arginine. Maximum growth rate (L), maximum density (M), and area under the curve (AUC) (N) are derived from OD600 measurements every 0.5h over 10 hours at 37C. Points represent strains with paired conditions connected with lines. Statistical significance was determined by paired t -test followed by Bonferroni correction. (O-Q) Protein expression of cytokines IL-8 (O), CCL20 (P) and IL-36γ (Q) in the basal media of Staphylococcus -colonized RHE was measured by ELISA (n=3-4 replicates per strain in an independent experiment colonizing 3 inflammatory and 4 commensal S. epidermidis strains and S. aureus as a reference). Points represent individual replicates colored by cluster and boxes show IQR with median. (R) Model summarizing species- and strain-level effects of Staphylococcus on RHE. Species-level differences distinguish commensal and pathogenic effects, while strain-level variation within S. epidermidis identifies inflammatory strains that activate a proinflammatory transcriptional program including upstream regulators (e.g. NFKB), chemokines (e.g., CCL20), cytokines (e.g., IL36γ) and downstream antimicrobial effectors (e.g. LCN2). Inflammatory S. epidermidis also enhances skin barrier defensive genes, particularly members of the LCE and SPRR gene families, which contribute to both barrier integrity and antimicrobial defense, and are regulated by transcription factors such as MAFB. These effects are linked to differences in gene content (e.g., cell surface genes), transcriptional programs, metabolism (e.g., arginine pathway), and pathogen-like growth phenotypes. P-values: **** p ≤ 0.0001, *** 0.0001 < p ≤ 0.001, ** 0.001 < p ≤ 0.01, * 0.01 < p ≤ 0.05., ns p > 0.05.

    Journal: bioRxiv

    Article Title: Species and strain diversity in Staphylococcus drive divergent host responses in human skin

    doi: 10.64898/2026.04.30.720712

    Figure Lengend Snippet: (A) Biofilm formation ability of commensal vs. inflammatory S. epidermidis strains. Biofilm positive strains were defined relative to a canonical biofilm-forming strain (Sep_1457). Numbers indicate strain counts per category and P-value and odds ratio calculated using Fisher’s exact test. (B-E) Survival ability in blood as measured by CFUs at 0, 2, and 24h of S. epidermidis strains (D) with respect to a bloodstream infection S. aureus strain (USA300LAC, (B)) with or without complement (+/-Comp) depletion (C, E). Points represent individual strains (or replicates for S. aureus) and boxes indicate IQR with median. Lines connect matched conditions. Significance was assessed by paired t-test with Bonferroni correction. (F-G) PCA plot of Staphylococcus cellular (F) or extracellular (G) metabolomes. Staphylococcus metabolomes were inferred by metabolic feature intensity within untargeted metabolomes. (H) Diagram of arginine metabolism pathway highlighting reactions catalyzed by arcA , argF , and argG . (I-K) Violin plots showing the metabolite abundance of arginine (I), carbamoyl-P (J), and argininosuccinate (K) in strains with or without arcA , argF , or argG. Points indicate normalized metabolite intensity; violins show distributions with embedded boxplots (median and IQR). Gene labels are black if acting on or downstream of the metabolite, grey otherwise. (L-N) Growth dynamics of inflammatory vs. commensal S. epidermidis strains in skin-like media with (+Arg) or without (-Arg) arginine. Maximum growth rate (L), maximum density (M), and area under the curve (AUC) (N) are derived from OD600 measurements every 0.5h over 10 hours at 37C. Points represent strains with paired conditions connected with lines. Statistical significance was determined by paired t -test followed by Bonferroni correction. (O-Q) Protein expression of cytokines IL-8 (O), CCL20 (P) and IL-36γ (Q) in the basal media of Staphylococcus -colonized RHE was measured by ELISA (n=3-4 replicates per strain in an independent experiment colonizing 3 inflammatory and 4 commensal S. epidermidis strains and S. aureus as a reference). Points represent individual replicates colored by cluster and boxes show IQR with median. (R) Model summarizing species- and strain-level effects of Staphylococcus on RHE. Species-level differences distinguish commensal and pathogenic effects, while strain-level variation within S. epidermidis identifies inflammatory strains that activate a proinflammatory transcriptional program including upstream regulators (e.g. NFKB), chemokines (e.g., CCL20), cytokines (e.g., IL36γ) and downstream antimicrobial effectors (e.g. LCN2). Inflammatory S. epidermidis also enhances skin barrier defensive genes, particularly members of the LCE and SPRR gene families, which contribute to both barrier integrity and antimicrobial defense, and are regulated by transcription factors such as MAFB. These effects are linked to differences in gene content (e.g., cell surface genes), transcriptional programs, metabolism (e.g., arginine pathway), and pathogen-like growth phenotypes. P-values: **** p ≤ 0.0001, *** 0.0001 < p ≤ 0.001, ** 0.001 < p ≤ 0.01, * 0.01 < p ≤ 0.05., ns p > 0.05.

    Article Snippet: Rabbit red blood cells with complement (Innovative Research Inc.; #IRBRBC10ML) was aliquoted into 96-well plates (150 μL per well).

    Techniques: Infection, Derivative Assay, Expressing, Enzyme-linked Immunosorbent Assay