antibodies against na v 1 7 α1 subunit (OriGene)

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OriGene antibodies against na v 1 7 α1 subunit

Na V 1.7 α1 subunit expression in wild-type (WT) and R192Q KI TG cultures. (A) Representative immunofluorescent examples of Na V 1.7 α1 expression in WT and R192Q KI mouse trigeminal ganglia (TG) cultures. Nuclei are visualized with DAPI (blue); scale bar = 20 μm. Note extensive co-staining of Na V 1.7 α1 (red) and β-tubulin III (green) protein in both WT and KI samples. (B) Western blot example showing protein expression of Na V 1.7 α1 in TG from P12 mice. β-actin was used as a loading control. Histograms representing Na V 1.7 α1 relative optical density values for each group (* p = 0.46, Mann-Whitney test; n = 8 experiments). Note the significant difference between WT and KI groups. (C) The histograms quantify the cell diameter distribution of Na V 1.7 α1 immunofluorescence in different subgroups of WT and KI cultures ( n = 3 experiments). The percentages of positive neurons for Na V 1.7 were calculated by considering the total number of neurons labeled by β-Tubulin III as standard.

Antibodies Against Na V 1 7 α1 Subunit, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickblock+western+blocking+buffer/SOX10+(BC002824)+Human+Untagged+Clone/pmc08185157-40-6-20
Average 90 stars, based on 3 article reviews

antibodies against na v 1 7 α1 subunit - by Bioz Stars, 2026-11

90/100 stars

Images

1) Product Images from "Overexpressed Na V 1.7 Channels Confer Hyperexcitability to in vitro Trigeminal Sensory Neurons of Ca V 2.1 Mutant Hemiplegic Migraine Mice"

Article Title: Overexpressed Na V 1.7 Channels Confer Hyperexcitability to in vitro Trigeminal Sensory Neurons of Ca V 2.1 Mutant Hemiplegic Migraine Mice

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2021.640709

Figure Legend Snippet: Na V 1.7 α1 subunit expression in wild-type (WT) and R192Q KI TG cultures. (A) Representative immunofluorescent examples of Na V 1.7 α1 expression in WT and R192Q KI mouse trigeminal ganglia (TG) cultures. Nuclei are visualized with DAPI (blue); scale bar = 20 μm. Note extensive co-staining of Na V 1.7 α1 (red) and β-tubulin III (green) protein in both WT and KI samples. (B) Western blot example showing protein expression of Na V 1.7 α1 in TG from P12 mice. β-actin was used as a loading control. Histograms representing Na V 1.7 α1 relative optical density values for each group (* p = 0.46, Mann-Whitney test; n = 8 experiments). Note the significant difference between WT and KI groups. (C) The histograms quantify the cell diameter distribution of Na V 1.7 α1 immunofluorescence in different subgroups of WT and KI cultures ( n = 3 experiments). The percentages of positive neurons for Na V 1.7 were calculated by considering the total number of neurons labeled by β-Tubulin III as standard.

Techniques Used: Expressing, Staining, Western Blot, MANN-WHITNEY, Immunofluorescence, Labeling

Na V 1.7 α1 subunit expression in WT and R192Q KI TG cultures after pretreatment with ω-agatoxin IVA. (A) Representative immunofluorescent examples of Na V 1.7 α1 expression in WT and R192Q KI mouse TG cultures. ω-agatoxin IVA (200 nM, overnight: in this and subsequent Figures the toxin is abbreviated as ω-Agatoxin) was used to specifically block Ca V 2.1 channels (that are mutated in the KI model). Nuclei are visualized with DAPI (blue); scale bar = 20 μm. Note extensive co-staining of Na V 1.7 α1 (red) and β-tubulin III (green) protein in both WT and KI samples. (B) Histograms represent the percentage of Na V 1.7-positive cells in WT and KI cultures. Note significant difference between WT and KI control groups (* p = 0.015, Mann–Whitney test; n = 7 experiments), and the decrease in Na V 1.7 positive neurons in the KI after application of ω-agatoxin IVA ( p = 0.035; n = 3 experiments). (C) The histograms quantify the cell diameter distribution of Na V 1.7 α1 immunofluorescence in different subgroups in WT and KI ω-agatoxin IVA-treated cultures.

Figure Legend Snippet: Na V 1.7 α1 subunit expression in WT and R192Q KI TG cultures after pretreatment with ω-agatoxin IVA. (A) Representative immunofluorescent examples of Na V 1.7 α1 expression in WT and R192Q KI mouse TG cultures. ω-agatoxin IVA (200 nM, overnight: in this and subsequent Figures the toxin is abbreviated as ω-Agatoxin) was used to specifically block Ca V 2.1 channels (that are mutated in the KI model). Nuclei are visualized with DAPI (blue); scale bar = 20 μm. Note extensive co-staining of Na V 1.7 α1 (red) and β-tubulin III (green) protein in both WT and KI samples. (B) Histograms represent the percentage of Na V 1.7-positive cells in WT and KI cultures. Note significant difference between WT and KI control groups (* p = 0.015, Mann–Whitney test; n = 7 experiments), and the decrease in Na V 1.7 positive neurons in the KI after application of ω-agatoxin IVA ( p = 0.035; n = 3 experiments). (C) The histograms quantify the cell diameter distribution of Na V 1.7 α1 immunofluorescence in different subgroups in WT and KI ω-agatoxin IVA-treated cultures.

Techniques Used: Expressing, Blocking Assay, Staining, MANN-WHITNEY, Immunofluorescence

Co-expression of Na V 1.7 α1 subunit and P2X3R in WT and R192Q KI cultures. (A) Representative examples of Na V 1.7 α1–P2X3 immunostaining in WT and R192Q KI mouse TG cultures. Nuclei are visualized with DAPI (blue); scale bar = 20 μm. Note the extensive co-staining of Na V 1.7 α1 (red) and P2X3 (green) protein in both WT and KI samples. (B) Histograms represent percentage co-expression of Na V 1.7 α1–P2X3 proteins in WT and KI cultures ( p = 0.95, Mann–Whitney test; n = 3 experiments). (C) The histograms quantify the cell diameter distribution of Na V 1.7 α1–P2X3R immunofluorescence in different subgroups in WT and KI cultures.

Figure Legend Snippet: Co-expression of Na V 1.7 α1 subunit and P2X3R in WT and R192Q KI cultures. (A) Representative examples of Na V 1.7 α1–P2X3 immunostaining in WT and R192Q KI mouse TG cultures. Nuclei are visualized with DAPI (blue); scale bar = 20 μm. Note the extensive co-staining of Na V 1.7 α1 (red) and P2X3 (green) protein in both WT and KI samples. (B) Histograms represent percentage co-expression of Na V 1.7 α1–P2X3 proteins in WT and KI cultures ( p = 0.95, Mann–Whitney test; n = 3 experiments). (C) The histograms quantify the cell diameter distribution of Na V 1.7 α1–P2X3R immunofluorescence in different subgroups in WT and KI cultures.

Techniques Used: Expressing, Immunostaining, Staining, MANN-WHITNEY, Immunofluorescence

Na V 1.7 current in WT and R192Q KI TG neurons. (A) Mean amplitudes of the currents evoked by a square pulse 100 ms depolarizing step from –75 to –45 mV, in WT and R192Q KI TG neurons under control conditions and after application of 1 μM TTX. Note larger control current in KI (* p = 0.039, two-sample Student’s t -test). Number of cells in each group: n = 29 (WT, control), n = 20 (WT, TTX), n = 43 (KI, control), i = 24 (KI, TTX). (B) Representative traces of inward currents recorded from one WT neuron in response to the same stimulation in control and after 1 μM TTX; the trace of TTX-sensitive current was obtained by subtraction of the TTX-resistant current from the control. (C) Superimposed representative traces of WT and KI TTX-sensitive currents obtained by subtraction; note larger KI current. (D) Histograms represent the calculated mean amplitudes of the currents evoked by the same stimulus (100-ms step from –75 to –45 mV) in control and after Tp1a (7 nM, 30 min). *Indicates statistically significant change (two-sample Student’s t -test, p -values are given in the text body). Number of cells in each group: n = 29 (WT, control), n = 28 (WT, Tp1a), n = 43 (KI, control), n = 38 (KI, Tp1a). (E) Superimposed representative traces of WT and KI Tp1a-sensitive (Na V 1.7) currents obtained by subtraction of the TTX-resistant current from the control; note larger Na V 1.7 current in KI.

Figure Legend Snippet: Na V 1.7 current in WT and R192Q KI TG neurons. (A) Mean amplitudes of the currents evoked by a square pulse 100 ms depolarizing step from –75 to –45 mV, in WT and R192Q KI TG neurons under control conditions and after application of 1 μM TTX. Note larger control current in KI (* p = 0.039, two-sample Student’s t -test). Number of cells in each group: n = 29 (WT, control), n = 20 (WT, TTX), n = 43 (KI, control), i = 24 (KI, TTX). (B) Representative traces of inward currents recorded from one WT neuron in response to the same stimulation in control and after 1 μM TTX; the trace of TTX-sensitive current was obtained by subtraction of the TTX-resistant current from the control. (C) Superimposed representative traces of WT and KI TTX-sensitive currents obtained by subtraction; note larger KI current. (D) Histograms represent the calculated mean amplitudes of the currents evoked by the same stimulus (100-ms step from –75 to –45 mV) in control and after Tp1a (7 nM, 30 min). *Indicates statistically significant change (two-sample Student’s t -test, p -values are given in the text body). Number of cells in each group: n = 29 (WT, control), n = 28 (WT, Tp1a), n = 43 (KI, control), n = 38 (KI, Tp1a). (E) Superimposed representative traces of WT and KI Tp1a-sensitive (Na V 1.7) currents obtained by subtraction of the TTX-resistant current from the control; note larger Na V 1.7 current in KI.

Techniques Used:

Infection:

Article Title: MT-100, a human Tie2-agonistic antibody, improves penile neurovasculature in diabetic mice via the novel target Srpx2.
Article Snippet: To test the ability of human Tie2-agonistic antibody (MT-100, provided by Mabtics Co., Ltd., South Korea, Daejeon) to alleviate erectile function in diabetes-induced ED mice, the STZ-induced type 1 diabetic mice received two intracavernous injections of PBS (Gibco, Carlsbad, CA, USA), NC (10 μg in 20 μL of PBS), or MT-100 (days −3 and 0; 1 μg, 10 μg, and 20 μg in 20 μL of PBS). .. For lentivirus infection, scrambled control shRNA (shCon) lentivirus particles (Santa Cruz Biotechnology, Dallas, TX, USA) or mouse lentivirus containing shRNA targeting Srpx2 (shSrpx2, OriGene Technologies Inc., Rockville, USA) were intracavernously injected at different transduction units (TU; 5 × 103 TU/mL, 2.5 × 104 TU/mL, 5 × 104 TU/mL lentivirus). ..

Control:

shRNA:

Injection:

Transduction:

other:

Article Title: ECM29/proteasome-mediated self-antigen generation by CNS-resident neuroglia promotes regulatory T cell activation.
Article Snippet: These primers, designed to encode 9 + 3 amino acids of the sequence corresponding to the predicted gene origin of the peptides, were conveniently ordered with 50-phosphorylation, enabling seamless ligation after enzymatic removal of vector phosphates by Antarctic Phosphatase (AnP, NEB).

Article Title: The intestinal fungus Aspergillus tubingensis promotes polycystic ovary syndrome through a secondary metabolite.
Article Snippet: Article The intestinal fungus Aspergillus tubingensis promotes polycystic ovary syndrome through a secondary metabolite

Article Title: Neutrophil extracellular traps promote pre-metastatic niche formation in the omentum by expanding innate-like B cells that express IL-10.
Article Snippet: VertX (B6(Cg)-Il10tm1.1Karp/J) mice, that express enhanced green fluorescent protein (eGFP) under the control of an internal ribosome entry site in the Il10 30-untranslated region,39 were purchased from the Jackson Laboratory.

Incubation:

Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis.
Article Snippet: To verify the presence of the fortilin-CTNNA3 interaction and also to determine whether fortilin interacts with CTNNA1, CTNNA2, and CTNNB in vivo, we performed the PLA6,9 using a commercially available kit (Sigma, Duolink® series) according to the manufacturer’s instructions. .. Briefly, we seeded U2OS cells into 2-chamber slides (Thermo-Fisher; Catalog #: 154461), incubated them for 24 h, fixed them with 4% paraformaldehyde, permeabilized themwith 0.1% Triton-X-100 in PBS, blocked them with Duolink Blocking solution at 37 °C for 1 h, and incubated them with (a) mouse anti-CTNNA3 mAb (1:200 dilution; OriGene; Catalog #: BM6021P), mouse anti-CTNNA1mAb (1:200 dilution; Abcam, Catalog #: ab231306), mouse anti-CTNNA2 pAb (1:200 dilution; Abnova (Taipei, Taiwan), Catalog #:H00001496-B01P), ormouse anti-CTNNBmAb (1:200 dilution; Sigma; Catalog #: C7207) and (b) rabbit anti-fortilin mAb (1:200 dilution; Abcam;Catalog #: ab133568) overnight at 4 °C. .. The nextmorning, after washing withWash Buffer (Sigma, Duolink®, Catalog #: DUO82049), we incubated the cellswith theDuolinkPLAprobes,whichwere anti-mouse and anti-rabbit mAbs conjugated to oligonucleotides (Sigma, Duolink® series; PLA probe anti-mouse MINUS, Catalog #: DUO92004 and PLA probe anti-rabbit PLUS, Catalog #: DUO92002) for 1 h at 37 °C.

Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis
Article Snippet: To verify the presence of the fortilin-CTNNA3 interaction and also to determine whether fortilin interacts with CTNNA1, CTNNA2, and CTNNB in vivo, we performed the PLA , using a commercially available kit (Sigma, Duolink® series) according to the manufacturer’s instructions. .. Briefly, we seeded U2OS cells into 2-chamber slides (Thermo-Fisher; Catalog #: 154461), incubated them for 24 h, fixed them with 4% paraformaldehyde, permeabilized them with 0.1% Triton-X-100 in PBS, blocked them with Duolink Blocking solution at 37 °C for 1 h, and incubated them with (a) mouse anti-CTNNA3 mAb (1:200 dilution; OriGene; Catalog #: BM6021P), mouse anti-CTNNA1 mAb (1:200 dilution; Abcam, Catalog #: ab231306), mouse anti-CTNNA2 pAb (1:200 dilution; Abnova (Taipei, Taiwan), Catalog #: H00001496-B01P), or mouse anti-CTNNB mAb (1:200 dilution; Sigma; Catalog #: C7207) and (b) rabbit anti-fortilin mAb (1:200 dilution; Abcam; Catalog #: ab133568) overnight at 4 °C. .. The next morning, after washing with Wash Buffer (Sigma, Duolink®, Catalog #: DUO82049), we incubated the cells with the Duolink PLA probes, which were anti-mouse and anti-rabbit mAbs conjugated to oligonucleotides (Sigma, Duolink® series; PLA probe anti-mouse MINUS, Catalog #: DUO92004 and PLA probe anti-rabbit PLUS, Catalog #: DUO92002) for 1 h at 37 °C.

Review