Review



signal solution 2  (Toyobo)


Bioz Verified Symbol Toyobo is a verified supplier
Bioz Manufacturer Symbol Toyobo manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    Toyobo signal solution 2
    Signal Solution 2, supplied by Toyobo, used in various techniques. Bioz Stars score: 98/100, based on 3812 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblock+western+blocking+buffer/Can+Get+Signal/pm26645585-48-34-37
    Average 98 stars, based on 3812 article reviews
    signal solution 2 - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Impact of Host α2-Antiplasmin Deficiency on Engraftment and the Morphological Maturation of Transplanted Neural Precursor Cells in the Mouse Brain.
    Article Snippet: Immunohistochemistry Floating sections were incubated in a blocking solution consisting of 0.3% Triton X-100 and 10% normal goat serum (Vector Laboratories, Burlingame, CA, U.S.A.) in PBS. .. The sections were then incubated with the following primary Abs in Can Get Signal Immunostain Immunoreaction Enhancer Solution B (Toyobo, Osaka, Japan) for 72 h at 4°C: rabbit anti-DCX Ab (1 : 300; ab18723; Abcam) and rabbit anti-NeuN Ab (1 : 1000; ABN78; Millipore, Temecula, CA, U.S.A.). .. The sections were washed with PBS and incubated with Alexa Fluor 546-conjugated goat anti-rabbit immunoglobulin G (1 : 1000; A11001, Thermo Fisher Scientific) for 2 h at r.t. After nuclear staining with DAPI (1 : 800; Dojindo), the sections were mounted on glass slides and imaged using a confocal laser scanning microscope (LSM800; Carl Zeiss).

    Article Title: BRD8 deficiency leads to progressive impairment of spermatogenesis in mice.
    Article Snippet: The sections were then blocked with 5% goat serum (Abcam, Cambridge, UK, Cat# ab7481, AB_2716553) in TBS containing 0.05% Tween-20 for 30 min at room temperature. .. Sections were incubated overnight at 4°C with anti-BRD8 antibody (Sigma-Aldrich, MO, Cat# HPA001841, AB_1845438, 1:1000) diluted in Can Get Signal Immunostain Solution A (Toyobo, Osaka, Japan). .. After washing, sections were incubated with an HRP-labeled secondary antibody using the EnVision+ System (Dako, Glostrup, Denmark, Cat# K5007).

    Article Title: ALIX-dependent small extracellular vesicles from tissue-resident mesenchymal cells regulate pancreatic β cell proliferation in obesity
    Article Snippet: The membranes were blocked with Blocking One (Nacalai Tesque, #03953-95) or Blocking One-P (Nacalai Tesque, #05999-84), followed by overnight incubation at 4°C with primary antibodies diluted in Can Get Signal Solution 1 (TOYOBO, #NKB-201). .. After being washed, the membranes were incubated for 1 hour at room temperature with HRP-conjugated secondary antibodies diluted in Can Get Signal Solution 2 (TOYOBO, #NKB-301). .. Protein signals were developed using Chemi-Lumi One Super (Nacalai Tesque, #02230-30) and visualized and quantified with a ChemiDoc Touch MP system and Image Lab software (Bio-Rad).

    Article Title: Acute trazodone treatment ameliorates behavioral abnormalities and improves subsequent short‐term memory in a lipopolysaccharide‐induced mouse model of acute brain dysfunction
    Article Snippet: Proteins were transferred onto 0.2‐μm polyvinylidene difluoride membranes (Trans‐Blot Turbo Transfer Pack; Bio‐Rad, 1704156) using the Trans‐Blot Turbo system (Bio‐Rad). .. Membranes were blocked with 5% skim milk in phosphate‐buffered saline containing 0.1% Tween 20 (PBST) at 4°C overnight and incubated overnight at 4°C with primary antibodies diluted in Can Get Signal Solution 1 (TOYOBO, NKB‐101). .. In the cerebral cortex and hippocampus, the microglial marker ionized calcium‐binding adaptor molecule 1 (Iba1), the astrocyte marker glial fibrillary acidic protein (GFAP), the neuronal marker neuronal nuclei (NeuN), and the inflammatory cytokines IL‐6 and TNF‐α were quantified using primary antibodies against Iba1 (Proteintech, 81728‐1‐RR; 1:2000), GFAP (Proteintech, 16825‐1‐AP; 1:5000), NeuN (Abcam, ab128886; 1:1000), IL‐6 (Proteintech, 29444‐1‐AP; 1:1000), and TNF‐α (Proteintech, 26162‐1‐AP; 1:1000), with β‐actin (Proteintech, 20536‐1‐AP; 1:2000) used as a loading control.

    Article Title: Fibroblast-like synoviocytes exacerbate autoimmune arthritis upon psychological stress in an IL-6–dependent manner
    Article Snippet: For antigen retrieval, slides were boiled in tris-EDTA (pH 9.0) antigen retrieval buffer (BioLegend, San Diego, CA) for 10 min. After antigen retrieval, slides were processed using the ImmPRESS HRP Universal PLUS Polymer Kit (Vector Laboratories, Newark, CA) according to the manufacturer’s protocol. .. Briefly, slides were blocked with 2.5% normal horse serum at room temperature for 30 min and then incubated with anti–IL-6 antibody (EPR23819-103, 1:100) (Abcam, Cambridge, UK) diluted in Can Get Signal Immunoreaction Enhancer Solution 1 (TOYOBO, Osaka, Japan) at room temperature for 1 hour. ..

    Saline:

    Article Title: Acute trazodone treatment ameliorates behavioral abnormalities and improves subsequent short‐term memory in a lipopolysaccharide‐induced mouse model of acute brain dysfunction
    Article Snippet: Proteins were transferred onto 0.2‐μm polyvinylidene difluoride membranes (Trans‐Blot Turbo Transfer Pack; Bio‐Rad, 1704156) using the Trans‐Blot Turbo system (Bio‐Rad). .. Membranes were blocked with 5% skim milk in phosphate‐buffered saline containing 0.1% Tween 20 (PBST) at 4°C overnight and incubated overnight at 4°C with primary antibodies diluted in Can Get Signal Solution 1 (TOYOBO, NKB‐101). .. In the cerebral cortex and hippocampus, the microglial marker ionized calcium‐binding adaptor molecule 1 (Iba1), the astrocyte marker glial fibrillary acidic protein (GFAP), the neuronal marker neuronal nuclei (NeuN), and the inflammatory cytokines IL‐6 and TNF‐α were quantified using primary antibodies against Iba1 (Proteintech, 81728‐1‐RR; 1:2000), GFAP (Proteintech, 16825‐1‐AP; 1:5000), NeuN (Abcam, ab128886; 1:1000), IL‐6 (Proteintech, 29444‐1‐AP; 1:1000), and TNF‐α (Proteintech, 26162‐1‐AP; 1:1000), with β‐actin (Proteintech, 20536‐1‐AP; 1:2000) used as a loading control.

    Blocking Assay:

    Article Title: Examination of the expression of G-protein-coupled receptor (GPR) 37 across whole-body organs using GPR37-deficient mice by Western blotting.
    Article Snippet: 103 104 Western blotting 105 Protein samples were mixed with Laemmli sample buffer (#1610747, Bio-Rad Laboratories, 106 Hercules, CA, USA) and 1% 2-mercaptoethanol and boiled at 95 °C for 5 min. Proteins were 107 separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) using Any kD Mini-108 PROTEAN TGX Precast Gels (Bio-Rad Laboratories) and subsequently transferred to PVDF 109 membranes (Trans-Blot Turbo Transfer Pack, PVDF (Mini), Bio-Rad Laboratories). .. 110 Blocking of membranes was conducted with PVDF Blocking Reagent for Can Get Signal 111 (TOYOBO Co., Ltd., Osaka, Japan) for 60 min at room temperature (RT). .. Primary antibody (GPR37 112 (D4C8H) Rabbit mAb #64407, Cell Signaling Technology, Danvers, MA, USA) was diluted 500 113 times in Can Get Signal Solution 1 (TOYOBO Co., Ltd.) and applied to membranes for 12 hr at 4 °C.



    Similar Products

    90
    Beyotime quickblock blocking buffer for western blotting
    Quickblock Blocking Buffer For Western Blotting, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblock+western+blocking+buffer/quickblocktm+blocking+buffer+for+western+blot/pm40598642-79-19-20
    Average 90 stars, based on 1 article reviews
    quickblock blocking buffer for western blotting - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Beyotime quickblock western blocking buffer p0252
    Quickblock Western Blocking Buffer P0252, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblock+western+blocking+buffer/quickblocktm+blocking+buffer+for+western+blot/pmc12116433-90-4-9
    Average 90 stars, based on 1 article reviews
    quickblock western blocking buffer p0252 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Beyotime quickblock western blocking buffer
    Quickblock Western Blocking Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblock+western+blocking+buffer/quickblocktm+blocking+buffer+for+western+blot/pm40310626-74-11-15
    Average 90 stars, based on 1 article reviews
    quickblock western blocking buffer - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Beyotime quickblock blocking buffer for western blot p0252
    Quickblock Blocking Buffer For Western Blot P0252, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblock+western+blocking+buffer/quickblocktm+blocking+buffer+for+western+blot/pm40275795-101-16-19
    Average 90 stars, based on 1 article reviews
    quickblock blocking buffer for western blot p0252 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Beyotime quickblock western blot blocking buffer
    Quickblock Western Blot Blocking Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblock+western+blocking+buffer/quickblocktm+blocking+buffer+for+western+blot/pm39828780-98-10-15
    Average 90 stars, based on 1 article reviews
    quickblock western blot blocking buffer - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results