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Promega quantustm fluoremeter
Quantustm Fluoremeter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantustm+fluorometer+dsdna+quantification+kit/quantustm+fluorimeter/pmc08117269-80-10-12
Average 90 stars, based on 1 article reviews
quantustm fluoremeter - by Bioz Stars, 2026-11
90/100 stars

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Article Title: The Extract from the Stem and Leaf of Paeonia lactiflora Pall Has Demonstrated an Anti-Oxidative Stress Effect in Alleviating Diarrhea by Regulating the Gut-Liver Axis
Article Snippet: The polymerase chain reaction (PCR) was conducted under the following stringent conditions: pre-denaturation at 95 °C for 3 min, followed by 27 cycles of denaturation at 95 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 10 min. PCR products were purified using 2% agarose gel electrophoresis and the AxyPrep DNA Gel Extraction Kit(AXYGEN Scientific, Union City, CA, USA), then quantified with the QuantusTM -ST Fluorometer(Promega Corporation, Madison, WI, USA).

Sequencing:

Article Title: Soil Physicochemical Properties and Rhizosphere Microorganisms Mediates the Growth of Cigar Tobacco Across Different Ecoregions in Yunnan, Southwest China
Article Snippet: .. Illumina MiSeq Sequencing Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, USA) was used to extract genomic DNA from 0.5g soil sample, and then the PCR productwas extracted from a 2% agarose gel and purified usingthe AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, USA).Diluted genomic DNA at a concentration of 1 ng/μL was used as template and PCR was performed using specific primers with Barcode and HiFi Hot Start Ready Mix high-fidelity enzymes from Qingke Biological Co., Ltd. to ensure amplification efficiency and accuracy, depending on the selected sequencing region. ..

Polymerase Chain Reaction:

Article Title: Soil Physicochemical Properties and Rhizosphere Microorganisms Mediates the Growth of Cigar Tobacco Across Different Ecoregions in Yunnan, Southwest China
Article Snippet: .. Illumina MiSeq Sequencing Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, USA) was used to extract genomic DNA from 0.5g soil sample, and then the PCR productwas extracted from a 2% agarose gel and purified usingthe AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, USA).Diluted genomic DNA at a concentration of 1 ng/μL was used as template and PCR was performed using specific primers with Barcode and HiFi Hot Start Ready Mix high-fidelity enzymes from Qingke Biological Co., Ltd. to ensure amplification efficiency and accuracy, depending on the selected sequencing region. ..

Article Title: Mechanisms of tomato growth promotion in three soils after applying Bacillus combinations
Article Snippet: The application of Bacillus combinations is a promising strategy to increase plant growth by increasing phosphorus availability in different soil environments.. In this study, we investigated the effects of Bacillus inoculation on tomato seedling growth in Haplic Chernozems, Haplic Calcisols, and Haplic Luvisols soils.. The results revealed that Bacillus colonization varied significantly among the soil types, with better colonization observed in the Haplic Calcisols and Haplic Luvisols soils, which was positively correlated with the total phosphorus content of the soil.

Article Title: Alterations in the Salivary Microbiome and Metabolism in Patients With Carotid Atherosclerosis from Rural Northeast China
Article Snippet: Bacterial DNA from saliva samples was extracted using the DNeasy PowerSoil Pro Kit (QIAGEN, Hilden, Germany). .. Polymerase chain reaction was performed using bacterial primers 27F (5′- AGRGTTYGATYMTGGCTCAG- 3′) and 1492R (5′- RGYTACCTTGTTACGACTT- 3′) to amplify the bacterial 16S rRNA genes.23 AMPure PB beads (Pacific Biosciences, Menlo Park, CA, USA) and QuantusTM Fluorometers (Promega, WI, USA) were used to purify and quantify polymerase chain reaction products. .. DNA library was constructed using the SMRTbell prep kit 3.0 (Pacific Biosciences, Menlo Park, CA, USA) and then applied to single- end sequencing on the PacBio Sequel IIe System (Pacific Biosciences, Menlo Park, CA, USA).

Article Title: Alterations in the Salivary Microbiome and Metabolism in Patients With Carotid Atherosclerosis from Rural Northeast China
Article Snippet: .. AMPure PB beads (Pacific Biosciences, Menlo Park, CA, USA) and QuantusTM Fluorometers (Promega, WI, USA) were used to purify and quantify polymerase chain reaction products. .. DNA library was constructed using the SMRTbell prep kit 3.0 (Pacific Biosciences, Menlo Park, CA, USA) and then applied to single‐end sequencing on the PacBio Sequel IIe System (Pacific Biosciences, Menlo Park, CA, USA).

Article Title: Effects of different fertilization treatments on coffee bean quality and rhizosphere microorganisms
Article Snippet: .. Analysis of the functional diversity of soil microorganisms Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, U.S.A.) was used to extract genomic DNA from 0.5 g soil sample, and then the PCR product was extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.A.) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, U.S.A.). ..

Agarose Gel Electrophoresis:

Article Title: Soil Physicochemical Properties and Rhizosphere Microorganisms Mediates the Growth of Cigar Tobacco Across Different Ecoregions in Yunnan, Southwest China
Article Snippet: .. Illumina MiSeq Sequencing Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, USA) was used to extract genomic DNA from 0.5g soil sample, and then the PCR productwas extracted from a 2% agarose gel and purified usingthe AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, USA).Diluted genomic DNA at a concentration of 1 ng/μL was used as template and PCR was performed using specific primers with Barcode and HiFi Hot Start Ready Mix high-fidelity enzymes from Qingke Biological Co., Ltd. to ensure amplification efficiency and accuracy, depending on the selected sequencing region. ..

Article Title: Mechanisms of tomato growth promotion in three soils after applying Bacillus combinations
Article Snippet: The application of Bacillus combinations is a promising strategy to increase plant growth by increasing phosphorus availability in different soil environments.. In this study, we investigated the effects of Bacillus inoculation on tomato seedling growth in Haplic Chernozems, Haplic Calcisols, and Haplic Luvisols soils.. The results revealed that Bacillus colonization varied significantly among the soil types, with better colonization observed in the Haplic Calcisols and Haplic Luvisols soils, which was positively correlated with the total phosphorus content of the soil.

Article Title: Effects of different fertilization treatments on coffee bean quality and rhizosphere microorganisms
Article Snippet: .. Analysis of the functional diversity of soil microorganisms Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, U.S.A.) was used to extract genomic DNA from 0.5 g soil sample, and then the PCR product was extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.A.) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, U.S.A.). ..

Purification:

Article Title: Soil Physicochemical Properties and Rhizosphere Microorganisms Mediates the Growth of Cigar Tobacco Across Different Ecoregions in Yunnan, Southwest China
Article Snippet: .. Illumina MiSeq Sequencing Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, USA) was used to extract genomic DNA from 0.5g soil sample, and then the PCR productwas extracted from a 2% agarose gel and purified usingthe AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, USA).Diluted genomic DNA at a concentration of 1 ng/μL was used as template and PCR was performed using specific primers with Barcode and HiFi Hot Start Ready Mix high-fidelity enzymes from Qingke Biological Co., Ltd. to ensure amplification efficiency and accuracy, depending on the selected sequencing region. ..

Article Title: Mechanisms of tomato growth promotion in three soils after applying Bacillus combinations
Article Snippet: The application of Bacillus combinations is a promising strategy to increase plant growth by increasing phosphorus availability in different soil environments.. In this study, we investigated the effects of Bacillus inoculation on tomato seedling growth in Haplic Chernozems, Haplic Calcisols, and Haplic Luvisols soils.. The results revealed that Bacillus colonization varied significantly among the soil types, with better colonization observed in the Haplic Calcisols and Haplic Luvisols soils, which was positively correlated with the total phosphorus content of the soil.

Article Title: Effects of different fertilization treatments on coffee bean quality and rhizosphere microorganisms
Article Snippet: .. Analysis of the functional diversity of soil microorganisms Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, U.S.A.) was used to extract genomic DNA from 0.5 g soil sample, and then the PCR product was extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.A.) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, U.S.A.). ..

Gel Extraction:

Article Title: Soil Physicochemical Properties and Rhizosphere Microorganisms Mediates the Growth of Cigar Tobacco Across Different Ecoregions in Yunnan, Southwest China
Article Snippet: .. Illumina MiSeq Sequencing Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, USA) was used to extract genomic DNA from 0.5g soil sample, and then the PCR productwas extracted from a 2% agarose gel and purified usingthe AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, USA).Diluted genomic DNA at a concentration of 1 ng/μL was used as template and PCR was performed using specific primers with Barcode and HiFi Hot Start Ready Mix high-fidelity enzymes from Qingke Biological Co., Ltd. to ensure amplification efficiency and accuracy, depending on the selected sequencing region. ..

Article Title: Effects of different fertilization treatments on coffee bean quality and rhizosphere microorganisms
Article Snippet: .. Analysis of the functional diversity of soil microorganisms Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, U.S.A.) was used to extract genomic DNA from 0.5 g soil sample, and then the PCR product was extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.A.) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, U.S.A.). ..

Concentration Assay:

Article Title: Soil Physicochemical Properties and Rhizosphere Microorganisms Mediates the Growth of Cigar Tobacco Across Different Ecoregions in Yunnan, Southwest China
Article Snippet: .. Illumina MiSeq Sequencing Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, USA) was used to extract genomic DNA from 0.5g soil sample, and then the PCR productwas extracted from a 2% agarose gel and purified usingthe AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, USA).Diluted genomic DNA at a concentration of 1 ng/μL was used as template and PCR was performed using specific primers with Barcode and HiFi Hot Start Ready Mix high-fidelity enzymes from Qingke Biological Co., Ltd. to ensure amplification efficiency and accuracy, depending on the selected sequencing region. ..

Amplification:

Article Title: Soil Physicochemical Properties and Rhizosphere Microorganisms Mediates the Growth of Cigar Tobacco Across Different Ecoregions in Yunnan, Southwest China
Article Snippet: .. Illumina MiSeq Sequencing Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, USA) was used to extract genomic DNA from 0.5g soil sample, and then the PCR productwas extracted from a 2% agarose gel and purified usingthe AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, USA).Diluted genomic DNA at a concentration of 1 ng/μL was used as template and PCR was performed using specific primers with Barcode and HiFi Hot Start Ready Mix high-fidelity enzymes from Qingke Biological Co., Ltd. to ensure amplification efficiency and accuracy, depending on the selected sequencing region. ..

DNA Purification:

Article Title: No Evidence of Neutrophil Response Modulation in Goats after Immunization against Paratuberculosis with a Heat-Inactivated Vaccine
Article Snippet: .. The DNA was extracted using the Maxwell ® 16 Cell DNA Purification Kit with the Maxwell 16 Instrument (Promega, Madison, WI, USA) and quantified using the QuantiFluorTM ONEdsDNA System kit (Promega, Madison, WI, USA) and QuantusTM Fluoremeter (Promega, Madison, WI, USA). ..

Functional Assay:

Article Title: Effects of different fertilization treatments on coffee bean quality and rhizosphere microorganisms
Article Snippet: .. Analysis of the functional diversity of soil microorganisms Fast DNA SPIN Kit For Soil (MP Biomedicals, LLC, U.S.A.) was used to extract genomic DNA from 0.5 g soil sample, and then the PCR product was extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.A.) according to the manufacturer’s instructions and quantified using a QuantusTMF Fluorometer (Promega, U.S.A.). ..



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