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cdna first strand synthesis kit  (tiangen biotech co)


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    Structured Review

    tiangen biotech co cdna first strand synthesis kit
    Cdna First Strand Synthesis Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantiscript+reverse+transcriptase+kit/miRNA+First-Strand+cDNA+Synthesis+Kit1/pmc10377183-66-14-21
    Average 96 stars, based on 726 article reviews
    cdna first strand synthesis kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: MYO1C is a urinary extracellular vesicle biomarker and mediator of podocyte injury in diabetic nephropathy
    Article Snippet: RNA concentrations were quantified using the Qubit 4.0 fluorometer with Qubit RNA Assay Kits ( Q32852 , ThermoFisher). .. For mRNA, complementary DNA (cDNA) was synthesized via reverse transcription using the PrimeScript RT Master Mix (RR036A, Takara, Japan). miRNA cDNA was prepared using the miReute Enhanced miRNA First-Strand Synthesis Kit (KR211, TIANGEN, China). qPCR was performed using the Premix Ex Taq ROX plus (RR39LR,Takara, Japan) to measure the relative expression levels of target genes. .. Primers and probes for mRNA and miRNA were designed and synthesized by Generalbiol (Chuzhou, China) and are detailed in .

    Reverse Transcription:

    Article Title: MYO1C is a urinary extracellular vesicle biomarker and mediator of podocyte injury in diabetic nephropathy
    Article Snippet: RNA concentrations were quantified using the Qubit 4.0 fluorometer with Qubit RNA Assay Kits ( Q32852 , ThermoFisher). .. For mRNA, complementary DNA (cDNA) was synthesized via reverse transcription using the PrimeScript RT Master Mix (RR036A, Takara, Japan). miRNA cDNA was prepared using the miReute Enhanced miRNA First-Strand Synthesis Kit (KR211, TIANGEN, China). qPCR was performed using the Premix Ex Taq ROX plus (RR39LR,Takara, Japan) to measure the relative expression levels of target genes. .. Primers and probes for mRNA and miRNA were designed and synthesized by Generalbiol (Chuzhou, China) and are detailed in .

    Article Title: Genomic locus of lncRNA- Gm26793 forms an inter-chromosomal interaction with Cubn to ensure proper stem cell differentiation in vitro and in vivo
    Article Snippet: .. Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reverse transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf) using Stormstar SYBR green qPCR master mix (DBI-2144). ..

    Article Title: OSW-1 inhibits tumor cell proliferation and migration via uncoupling protein 2 in hepatocellular carcinoma
    Article Snippet: RNA was extracted using the TRNzol Universal method (DP424; Tiangen Biotech Co., Ltd.). .. Reverse transcription was performed with the FastKing cDNA First Strand Synthesis Kit (KR116; Tiangen Biotech Co., Ltd.) under the following conditions: 42°C for 15 min followed by 95°C for 3 min. Quantitative PCR was performed using a Thermo 7300 Plus PCR instrument (Thermo Fisher Scientific) with the SuperReal Fluorescence Quantitative PreMix Enhanced kit (FP205; Tiangen Biotech Co., Ltd.). ..

    Real-time Polymerase Chain Reaction:

    Article Title: MYO1C is a urinary extracellular vesicle biomarker and mediator of podocyte injury in diabetic nephropathy
    Article Snippet: RNA concentrations were quantified using the Qubit 4.0 fluorometer with Qubit RNA Assay Kits ( Q32852 , ThermoFisher). .. For mRNA, complementary DNA (cDNA) was synthesized via reverse transcription using the PrimeScript RT Master Mix (RR036A, Takara, Japan). miRNA cDNA was prepared using the miReute Enhanced miRNA First-Strand Synthesis Kit (KR211, TIANGEN, China). qPCR was performed using the Premix Ex Taq ROX plus (RR39LR,Takara, Japan) to measure the relative expression levels of target genes. .. Primers and probes for mRNA and miRNA were designed and synthesized by Generalbiol (Chuzhou, China) and are detailed in .

    Article Title: Cuproptosis-related gene signatures define the immune microenvironment in diabetic nephropathy
    Article Snippet: .. We used TRIpure Reagent (Aidlab, China) to extract total RNA from cells, reversed transcribe it with FastKing cDNA first-strand synthesis kit(TIANGEN, China) and used SuperReal enhanced version of the fluorometric premixed reagent (TIANGEN, China) for quantitative real-time PCR. ..

    Article Title: Supporting Information for Cellular redox homeostasis maintained by malic enzyme 2 is essential for MYC-driven T-cell lymphomagenesis
    Article Snippet: Quantitative PCR analysis Total RNA was isolated with RNA Simple Total RNA Kit (TIANGEN, #DP419). .. 1μg RNA per sample was reversed to complementary cDNA through first-strand cDNA Synthesis System (TIANGEN, #KR116). cDNA of each sample was used as template for quantitative PCR. ..

    Article Title: Kudiezi injection attenuates apoptosis and neuroinflammation for ameliorating angiogenesis via miR-21/PDCD4/NF-κB axis in MCAO/reperfusion rats.
    Article Snippet: Ethnopharmacological relevance: miR-21 has multiple neuroprotective effects in central nervous system (CNS) ischemic damage.. Kudiezi (KDZ), a traditional Chinese medication, has a longstanding application in cerebral ischemia treatment and has been clinically confirmed to be effective for ischemic injury.. However, its specific effects on miR-21 regulation are yet unclear.

    Article Title: Genomic locus of lncRNA- Gm26793 forms an inter-chromosomal interaction with Cubn to ensure proper stem cell differentiation in vitro and in vivo
    Article Snippet: .. Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reverse transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf) using Stormstar SYBR green qPCR master mix (DBI-2144). ..

    Article Title: miR-423–5p/NDUFS7-mediated mitochondrial function modulation contributes to quercetin-induced attenuation of pulmonary fibrosis via extracellular matrix remodeling regulation
    Article Snippet: Band intensities were quantified via ImageJ software. .. For miRNA analysis, cDNA synthesis was performed via the miRcute Enhanced miRNA cDNA First-Strand Synthesis Kit (Cat. No. KR211, TIANGEN), followed by quantitative real-time PCR via the miRcute Enhanced miRNA Fluorescence Quantitative Detection Kit (Cat. No. FP411, TIANGEN). .. For miRNA analysis, cDNA synthesis was performed via the miRcute Enhanced miRNA cDNA First-Strand Synthesis Kit (Cat. No. KR211, TIANGEN), followed by quantitative real-time PCR via the miRcute Enhanced miRNA Fluorescence Quantitative Detection Kit (Cat. No. FP411, TIANGEN).

    Article Title: OSW-1 inhibits tumor cell proliferation and migration via uncoupling protein 2 in hepatocellular carcinoma
    Article Snippet: RNA was extracted using the TRNzol Universal method (DP424; Tiangen Biotech Co., Ltd.). .. Reverse transcription was performed with the FastKing cDNA First Strand Synthesis Kit (KR116; Tiangen Biotech Co., Ltd.) under the following conditions: 42°C for 15 min followed by 95°C for 3 min. Quantitative PCR was performed using a Thermo 7300 Plus PCR instrument (Thermo Fisher Scientific) with the SuperReal Fluorescence Quantitative PreMix Enhanced kit (FP205; Tiangen Biotech Co., Ltd.). ..

    Expressing:

    Article Title: MYO1C is a urinary extracellular vesicle biomarker and mediator of podocyte injury in diabetic nephropathy
    Article Snippet: RNA concentrations were quantified using the Qubit 4.0 fluorometer with Qubit RNA Assay Kits ( Q32852 , ThermoFisher). .. For mRNA, complementary DNA (cDNA) was synthesized via reverse transcription using the PrimeScript RT Master Mix (RR036A, Takara, Japan). miRNA cDNA was prepared using the miReute Enhanced miRNA First-Strand Synthesis Kit (KR211, TIANGEN, China). qPCR was performed using the Premix Ex Taq ROX plus (RR39LR,Takara, Japan) to measure the relative expression levels of target genes. .. Primers and probes for mRNA and miRNA were designed and synthesized by Generalbiol (Chuzhou, China) and are detailed in .

    cDNA Synthesis:

    Article Title: Supporting Information for Cellular redox homeostasis maintained by malic enzyme 2 is essential for MYC-driven T-cell lymphomagenesis
    Article Snippet: Quantitative PCR analysis Total RNA was isolated with RNA Simple Total RNA Kit (TIANGEN, #DP419). .. 1μg RNA per sample was reversed to complementary cDNA through first-strand cDNA Synthesis System (TIANGEN, #KR116). cDNA of each sample was used as template for quantitative PCR. ..

    Article Title: miR-423–5p/NDUFS7-mediated mitochondrial function modulation contributes to quercetin-induced attenuation of pulmonary fibrosis via extracellular matrix remodeling regulation
    Article Snippet: Band intensities were quantified via ImageJ software. .. For miRNA analysis, cDNA synthesis was performed via the miRcute Enhanced miRNA cDNA First-Strand Synthesis Kit (Cat. No. KR211, TIANGEN), followed by quantitative real-time PCR via the miRcute Enhanced miRNA Fluorescence Quantitative Detection Kit (Cat. No. FP411, TIANGEN). .. For miRNA analysis, cDNA synthesis was performed via the miRcute Enhanced miRNA cDNA First-Strand Synthesis Kit (Cat. No. KR211, TIANGEN), followed by quantitative real-time PCR via the miRcute Enhanced miRNA Fluorescence Quantitative Detection Kit (Cat. No. FP411, TIANGEN).

    Article Title: Potassium uptake function of LbKT1 and LbSKOR from Lycium barbarum and their influence on the arbuscular mycorrhizal symbiosis.
    Article Snippet: Potassium participates in a variety of plant physiological processes and has great impact on plant growth and stress adaptation.. The absorption of potassium by Plant is mediated by potassium channels and transporters, and the Shaker potassium channel gene family plays an important role in potassium uptake.. Arbuscular mycorrhizal (AM) fungi form ubiquitous symbioses with plants and increase plants’ potassium uptake.

    Concentration Assay:

    Article Title: Kudiezi injection attenuates apoptosis and neuroinflammation for ameliorating angiogenesis via miR-21/PDCD4/NF-κB axis in MCAO/reperfusion rats.
    Article Snippet: Ethnopharmacological relevance: miR-21 has multiple neuroprotective effects in central nervous system (CNS) ischemic damage.. Kudiezi (KDZ), a traditional Chinese medication, has a longstanding application in cerebral ischemia treatment and has been clinically confirmed to be effective for ischemic injury.. However, its specific effects on miR-21 regulation are yet unclear.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Kudiezi injection attenuates apoptosis and neuroinflammation for ameliorating angiogenesis via miR-21/PDCD4/NF-κB axis in MCAO/reperfusion rats.
    Article Snippet: Ethnopharmacological relevance: miR-21 has multiple neuroprotective effects in central nervous system (CNS) ischemic damage.. Kudiezi (KDZ), a traditional Chinese medication, has a longstanding application in cerebral ischemia treatment and has been clinically confirmed to be effective for ischemic injury.. However, its specific effects on miR-21 regulation are yet unclear.

    Cell Culture:

    Article Title: Genomic locus of lncRNA- Gm26793 forms an inter-chromosomal interaction with Cubn to ensure proper stem cell differentiation in vitro and in vivo
    Article Snippet: .. Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reverse transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf) using Stormstar SYBR green qPCR master mix (DBI-2144). ..

    SYBR Green Assay:

    Article Title: Genomic locus of lncRNA- Gm26793 forms an inter-chromosomal interaction with Cubn to ensure proper stem cell differentiation in vitro and in vivo
    Article Snippet: .. Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reverse transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf) using Stormstar SYBR green qPCR master mix (DBI-2144). ..

    Fluorescence:

    Article Title: miR-423–5p/NDUFS7-mediated mitochondrial function modulation contributes to quercetin-induced attenuation of pulmonary fibrosis via extracellular matrix remodeling regulation
    Article Snippet: Band intensities were quantified via ImageJ software. .. For miRNA analysis, cDNA synthesis was performed via the miRcute Enhanced miRNA cDNA First-Strand Synthesis Kit (Cat. No. KR211, TIANGEN), followed by quantitative real-time PCR via the miRcute Enhanced miRNA Fluorescence Quantitative Detection Kit (Cat. No. FP411, TIANGEN). .. For miRNA analysis, cDNA synthesis was performed via the miRcute Enhanced miRNA cDNA First-Strand Synthesis Kit (Cat. No. KR211, TIANGEN), followed by quantitative real-time PCR via the miRcute Enhanced miRNA Fluorescence Quantitative Detection Kit (Cat. No. FP411, TIANGEN).

    Article Title: OSW-1 inhibits tumor cell proliferation and migration via uncoupling protein 2 in hepatocellular carcinoma
    Article Snippet: RNA was extracted using the TRNzol Universal method (DP424; Tiangen Biotech Co., Ltd.). .. Reverse transcription was performed with the FastKing cDNA First Strand Synthesis Kit (KR116; Tiangen Biotech Co., Ltd.) under the following conditions: 42°C for 15 min followed by 95°C for 3 min. Quantitative PCR was performed using a Thermo 7300 Plus PCR instrument (Thermo Fisher Scientific) with the SuperReal Fluorescence Quantitative PreMix Enhanced kit (FP205; Tiangen Biotech Co., Ltd.). ..

    Polymerase Chain Reaction:

    Article Title: OSW-1 inhibits tumor cell proliferation and migration via uncoupling protein 2 in hepatocellular carcinoma
    Article Snippet: RNA was extracted using the TRNzol Universal method (DP424; Tiangen Biotech Co., Ltd.). .. Reverse transcription was performed with the FastKing cDNA First Strand Synthesis Kit (KR116; Tiangen Biotech Co., Ltd.) under the following conditions: 42°C for 15 min followed by 95°C for 3 min. Quantitative PCR was performed using a Thermo 7300 Plus PCR instrument (Thermo Fisher Scientific) with the SuperReal Fluorescence Quantitative PreMix Enhanced kit (FP205; Tiangen Biotech Co., Ltd.). ..



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