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Bio-Quant inc bioquant life software
A) Monocyte migration was assayed using 48-well micro-chemotaxis chambers with the addition of monocytes (106/ml) and TLF in the upper and lower chambers, respectively. After 2 h of incubation, the membrane was scraped, fixed, and stained. Migrated monocytes were counted using <t>Bioquant</t> Life Science software. Results shown are the average +SD of seven experiments. *=p<0.01. B) Dose-response of monocyte migration to TLF. One experiment out of three is shown. C) Checkerboard analysis. Monocytes were allowed to migrate in the presence (+) of 1 μg/ml of TLF in the lower chamber or in both the upper and lower chambers. The results shown are the average (mean+SD) of three experiments. D) Effect of pertussis toxin (PTx). Monocytes were pretreated with 100 ng/ml PTx for 1 h at 37°C prior to utilization in the migration assay. 100ng/ml of CCL2 was used as a positive control for monocyte migration and to confirm the effect of PTx. The data show the average (mean+SD) of 4 experiments. *p<0.01; **p<0.05.
Bioquant Life Software, supplied by Bio-Quant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quant+software/bioquant+software/pmc02408856-84-19-22
Average 90 stars, based on 1 article reviews
bioquant life software - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "Lactoferrin acts as an alarmin to promote the recruitment and activation of antigen-presenting cells and antigen-specific immune responses"

Article Title: Lactoferrin acts as an alarmin to promote the recruitment and activation of antigen-presenting cells and antigen-specific immune responses

Journal:

doi:

A) Monocyte migration was assayed using 48-well micro-chemotaxis chambers with the addition of monocytes (106/ml) and TLF in the upper and lower chambers, respectively. After 2 h of incubation, the membrane was scraped, fixed, and stained. Migrated monocytes were counted using Bioquant Life Science software. Results shown are the average +SD of seven experiments. *=p<0.01. B) Dose-response of monocyte migration to TLF. One experiment out of three is shown. C) Checkerboard analysis. Monocytes were allowed to migrate in the presence (+) of 1 μg/ml of TLF in the lower chamber or in both the upper and lower chambers. The results shown are the average (mean+SD) of three experiments. D) Effect of pertussis toxin (PTx). Monocytes were pretreated with 100 ng/ml PTx for 1 h at 37°C prior to utilization in the migration assay. 100ng/ml of CCL2 was used as a positive control for monocyte migration and to confirm the effect of PTx. The data show the average (mean+SD) of 4 experiments. *p<0.01; **p<0.05.
Figure Legend Snippet: A) Monocyte migration was assayed using 48-well micro-chemotaxis chambers with the addition of monocytes (106/ml) and TLF in the upper and lower chambers, respectively. After 2 h of incubation, the membrane was scraped, fixed, and stained. Migrated monocytes were counted using Bioquant Life Science software. Results shown are the average +SD of seven experiments. *=p<0.01. B) Dose-response of monocyte migration to TLF. One experiment out of three is shown. C) Checkerboard analysis. Monocytes were allowed to migrate in the presence (+) of 1 μg/ml of TLF in the lower chamber or in both the upper and lower chambers. The results shown are the average (mean+SD) of three experiments. D) Effect of pertussis toxin (PTx). Monocytes were pretreated with 100 ng/ml PTx for 1 h at 37°C prior to utilization in the migration assay. 100ng/ml of CCL2 was used as a positive control for monocyte migration and to confirm the effect of PTx. The data show the average (mean+SD) of 4 experiments. *p<0.01; **p<0.05.

Techniques Used: Migration, Chemotaxis Assay, Incubation, Staining, Software, Positive Control

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Article Title: Loss of Vhl alters trabecular bone loss during S. aureus osteomyelitis in a cell-specific manner
Article Snippet: .. TRAP-stained slides were imaged with a Leica SCN400 Slide Scanner (Leica Biosystems) in brightfield at 20X or on a Cytation 5 Imaging System [BioTek Instruments, Winooski, VT, USA] in brightfield at 10X and analyzed with Bioquant software (Bioquant Image Analysis Corporation, Nashville, TN, USA). ..

Software:

Article Title: Loss of Vhl alters trabecular bone loss during S. aureus osteomyelitis in a cell-specific manner
Article Snippet: .. TRAP-stained slides were imaged with a Leica SCN400 Slide Scanner (Leica Biosystems) in brightfield at 20X or on a Cytation 5 Imaging System [BioTek Instruments, Winooski, VT, USA] in brightfield at 10X and analyzed with Bioquant software (Bioquant Image Analysis Corporation, Nashville, TN, USA). ..

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Article Snippet: .. The stained slides were imaged at ×20 magnification, and the percentage of fibrosis was quantified as the collagenpositive tissue of the total stained LV tissue using the Bioquant software (BIOQUANT Image Analysis Corporation, Nashville, TN), as described previously.11 A semiquantitative score was used to assess myocardial inflammation (0, no infiltrate; 1+, infiltrates involving <25% myocardium; 2+, infiltrates involving 25%–50%; 3+, infiltrates involving 50%–75% of the myocardium; and 4+, infiltrates involving 75%–100% of the myocardium), as described previously.23 The investigators performing all the above measurements and data analysis were blinded to group assignment to avoid bias. .. LV tissue was first minced using a sterile razor blade and then digested for 1.5 hours at 37 °C with the following combination of enzymes: collagenase XI (2500 U/g of tissue), DNase I (2000 U/g of tissue), hyaluronidase (2600 U/g of tissue; Millipore Sigma, St. Louis, MO), and collagenase I (1200 U/g of tissue; Worthington Biochemical Corporation, Lakewood, NJ).

Article Title: Deficiency of Trps1 in Cementoblasts Impairs Cementogenesis and Tooth Root Formation.
Article Snippet: Images of TB- and PR-stained sections were captured by Zeiss AxioCam on a Zeiss Axioskop A1 microscope and Nikon TE2000 microscope with polarizing filters, respectively. .. BIOQUANT software (BIOQUANT Image Analysis Corporation, Nashville, TN USA) was used to measure cementum area and alignment of collagen fibers. ..

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Staining:

Article Title: Transcutaneous Vagus Nerve Stimulation Ameliorates the Phenotype of Heart Failure With Preserved Ejection Fraction Through Its Anti-Inflammatory Effects
Article Snippet: .. The stained slides were imaged at ×20 magnification, and the percentage of fibrosis was quantified as the collagenpositive tissue of the total stained LV tissue using the Bioquant software (BIOQUANT Image Analysis Corporation, Nashville, TN), as described previously.11 A semiquantitative score was used to assess myocardial inflammation (0, no infiltrate; 1+, infiltrates involving <25% myocardium; 2+, infiltrates involving 25%–50%; 3+, infiltrates involving 50%–75% of the myocardium; and 4+, infiltrates involving 75%–100% of the myocardium), as described previously.23 The investigators performing all the above measurements and data analysis were blinded to group assignment to avoid bias. .. LV tissue was first minced using a sterile razor blade and then digested for 1.5 hours at 37 °C with the following combination of enzymes: collagenase XI (2500 U/g of tissue), DNase I (2000 U/g of tissue), hyaluronidase (2600 U/g of tissue; Millipore Sigma, St. Louis, MO), and collagenase I (1200 U/g of tissue; Worthington Biochemical Corporation, Lakewood, NJ).



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