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ptyb21 expression vector  (New England Biolabs)


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    Structured Review

    New England Biolabs ptyb21 expression vector
    Ptyb21 Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptyb21/pTYB21+Vector/pm36637620-41-6-9
    Average 94 stars, based on 87 article reviews
    ptyb21 expression vector - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis.
    Article Snippet: The TABLE 2 Rhodococcus equi strains used in this study Strain name Source vap gene Description Reference ATCC33701 A pneumonic foal vapA VapA-producing strain (34) U19 Tracheal lavage fluid of a Friesian horse vapA VapA-producing strain (35) S4 Mandibular lymph node of a pig vapB VapB-producing strain (36, 37) JCM94-3 AIDS patients in Switzerland vapN VapN-producing strain (14, 15) JID03-27 AIDS patients in Thailand vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-25 Renal transplant patients in the USA vapN VapN-producing strain (14, 15) JCM94-27 Lymphosarcoma patients in New Zealand vapN VapN-producing strain (14, 15) JCM94-14 AIDS patients in Australia vapN Weakly VapN-producing strain (14, 15) JCM94-16 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-31 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) November/December 2023 Volume 11 Issue 6 10.1128/spectrum.00729-23 11 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 14 J an ua ry 2 02 4 by 1 14 .4 .2 14 .1 75 . vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sapI and EcoRI (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    Article Title: The connection of α- and β-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties.
    Article Snippet: .. Preparation of protein expression vectors The pTYB21 vector belonging to the Intein Mediated Purification with an Affinity Chitin-binding Tag system (IMPACT, New England BioLabs) was chosen for expression in bacterial cells. .. The codon optimized sequence for the β-villin gene was synthesized by ATG: Biosynthetics and was delivered in the pUC vector, which was subcloned in the pTYB21 vector.

    Agarose Gel Electrophoresis:

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis.
    Article Snippet: The TABLE 2 Rhodococcus equi strains used in this study Strain name Source vap gene Description Reference ATCC33701 A pneumonic foal vapA VapA-producing strain (34) U19 Tracheal lavage fluid of a Friesian horse vapA VapA-producing strain (35) S4 Mandibular lymph node of a pig vapB VapB-producing strain (36, 37) JCM94-3 AIDS patients in Switzerland vapN VapN-producing strain (14, 15) JID03-27 AIDS patients in Thailand vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-25 Renal transplant patients in the USA vapN VapN-producing strain (14, 15) JCM94-27 Lymphosarcoma patients in New Zealand vapN VapN-producing strain (14, 15) JCM94-14 AIDS patients in Australia vapN Weakly VapN-producing strain (14, 15) JCM94-16 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-31 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) November/December 2023 Volume 11 Issue 6 10.1128/spectrum.00729-23 11 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 14 J an ua ry 2 02 4 by 1 14 .4 .2 14 .1 75 . vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sapI and EcoRI (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    Gel Extraction:

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis.
    Article Snippet: The TABLE 2 Rhodococcus equi strains used in this study Strain name Source vap gene Description Reference ATCC33701 A pneumonic foal vapA VapA-producing strain (34) U19 Tracheal lavage fluid of a Friesian horse vapA VapA-producing strain (35) S4 Mandibular lymph node of a pig vapB VapB-producing strain (36, 37) JCM94-3 AIDS patients in Switzerland vapN VapN-producing strain (14, 15) JID03-27 AIDS patients in Thailand vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-25 Renal transplant patients in the USA vapN VapN-producing strain (14, 15) JCM94-27 Lymphosarcoma patients in New Zealand vapN VapN-producing strain (14, 15) JCM94-14 AIDS patients in Australia vapN Weakly VapN-producing strain (14, 15) JCM94-16 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-31 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) November/December 2023 Volume 11 Issue 6 10.1128/spectrum.00729-23 11 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 14 J an ua ry 2 02 4 by 1 14 .4 .2 14 .1 75 . vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sapI and EcoRI (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    other:

    Article Title: Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3.
    Article Snippet: Ampicillin, chloramphenicol, 1,4-dithiothreitol (DTT) and Tris base were from Roth, pTYB21 vector and chitin resin from New England BioLabs, Chelex 100 resin from Bio-Rad, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES), 5,5ʹ-dithiobis-(2-nitrobenzoic acid) (DTNB) from TCI Europe.

    Article Title: Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3
    Article Snippet: Ampicillin, chloramphenicol, 1,4-dithiothreitol (DTT) and Tris base were from Roth, pTYB21 vector and chitin resin from New England BioLabs, Chelex 100 resin from Bio-Rad, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES), 5,5ʹ-dithiobis-(2-nitrobenzoic acid) (DTNB) from TCI Europe.

    Expressing:

    Article Title: The connection of α- and β-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties.
    Article Snippet: .. Preparation of protein expression vectors The pTYB21 vector belonging to the Intein Mediated Purification with an Affinity Chitin-binding Tag system (IMPACT, New England BioLabs) was chosen for expression in bacterial cells. .. The codon optimized sequence for the β-villin gene was synthesized by ATG: Biosynthetics and was delivered in the pUC vector, which was subcloned in the pTYB21 vector.

    Plasmid Preparation:

    Article Title: The connection of α- and β-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties.
    Article Snippet: .. Preparation of protein expression vectors The pTYB21 vector belonging to the Intein Mediated Purification with an Affinity Chitin-binding Tag system (IMPACT, New England BioLabs) was chosen for expression in bacterial cells. .. The codon optimized sequence for the β-villin gene was synthesized by ATG: Biosynthetics and was delivered in the pUC vector, which was subcloned in the pTYB21 vector.



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