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protoscript first strand cdna synthesis kit  (New England Biolabs)


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    Structured Review

    New England Biolabs protoscript first strand cdna synthesis kit
    a , Summary of EBV DNA ddPCR results from CSF supernatant in which EBER2 was normalized to a housekeeping gene (MS/CIS, n = 13; HC/OND, n = 5). b , EBV <t>cDNA</t> for each of the indicated genes were measured by ddPCR and normalized to a housekeeping gene. Each sample was run in duplicate and each dot represents the average result from each study participant. Data are the mean ± s.e.m. MS/CIS and HC/OND samples were compared using an unpaired two-tailed Student’s t -test with Welch’s correction; NS, not significant; n = 13 for MS/CIS for all genes except EBER2 where n = 12 due to lack of sufficient sample for MS27 and n = 5 in HC/OND for all genes except BamHI-W where n = 4 due to a lack of sufficient sample for OND4.
    Protoscript First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+kit/ProtoScript+First+Strand+cDNA+Synthesis+Kit/pmc12956596-392-6-12
    Average 97 stars, based on 2012 article reviews
    protoscript first strand cdna synthesis kit - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Antigen specificity of clonally enriched CD8 + T cells in multiple sclerosis"

    Article Title: Antigen specificity of clonally enriched CD8 + T cells in multiple sclerosis

    Journal: Nature Immunology

    doi: 10.1038/s41590-025-02412-3

    a , Summary of EBV DNA ddPCR results from CSF supernatant in which EBER2 was normalized to a housekeeping gene (MS/CIS, n = 13; HC/OND, n = 5). b , EBV cDNA for each of the indicated genes were measured by ddPCR and normalized to a housekeeping gene. Each sample was run in duplicate and each dot represents the average result from each study participant. Data are the mean ± s.e.m. MS/CIS and HC/OND samples were compared using an unpaired two-tailed Student’s t -test with Welch’s correction; NS, not significant; n = 13 for MS/CIS for all genes except EBER2 where n = 12 due to lack of sufficient sample for MS27 and n = 5 in HC/OND for all genes except BamHI-W where n = 4 due to a lack of sufficient sample for OND4.
    Figure Legend Snippet: a , Summary of EBV DNA ddPCR results from CSF supernatant in which EBER2 was normalized to a housekeeping gene (MS/CIS, n = 13; HC/OND, n = 5). b , EBV cDNA for each of the indicated genes were measured by ddPCR and normalized to a housekeeping gene. Each sample was run in duplicate and each dot represents the average result from each study participant. Data are the mean ± s.e.m. MS/CIS and HC/OND samples were compared using an unpaired two-tailed Student’s t -test with Welch’s correction; NS, not significant; n = 13 for MS/CIS for all genes except EBER2 where n = 12 due to lack of sufficient sample for MS27 and n = 5 in HC/OND for all genes except BamHI-W where n = 4 due to a lack of sufficient sample for OND4.

    Techniques Used: Two Tailed Test

    Related Articles

    RNA Extraction:

    Article Title: Spectrin coordinates cell shape and signaling essential for epidermal differentiation
    Article Snippet: Formation of TER was measured over time using an automated cell monitoring system (cellZscope, nanoAnalytics). .. RNA was extracted from samples using a Direct-zol RNA extraction kit (R2060; Zymo Research), and equal amounts of RNA were reverse transcribed using the ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs). .. Semiquantitative PCR was conducted using a StepOnePlus System (Thermo Fisher Scientific).

    Reverse Transcription:

    Article Title: Spectrin coordinates cell shape and signaling essential for epidermal differentiation
    Article Snippet: Formation of TER was measured over time using an automated cell monitoring system (cellZscope, nanoAnalytics). .. RNA was extracted from samples using a Direct-zol RNA extraction kit (R2060; Zymo Research), and equal amounts of RNA were reverse transcribed using the ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs). .. Semiquantitative PCR was conducted using a StepOnePlus System (Thermo Fisher Scientific).

    Article Title: Japanese soil-borne wheat mosaic virus CP-RT protein associates with the endoplasmic reticulum network and mobile particles at the ER-Golgi interface during infection.
    Article Snippet: 100 mg leaf material ground in liquid nitrogen using a TissueLyser (Qiagen) was processed with the RNeasy Plant Mini kit (Qiagen). .. Alternatively, RNA was extracted from roots and leaves of infected barley plants using NucleoZol (Macherey-Nagel). cDNA was synthesized using random primers and reverse transcriptase (ProtoScript First Strand cDNA Synthesis Kit, NEB). ..

    Article Title: SiO₂ exposure triggers NOX1/ROS-dependent epithelial necroptosis and drives Th17 cell activation to initiate pneumonia in mice.
    Article Snippet: SiO2 (silicon dioxide) particles have been classified as one of the most hazardous environmental pollutants for the respiratory system, yet their hazards are often overlooked.. The abnormal activation of Th17 helper T cells that produce interleukin-17 (IL-17) is associated with the development of multiple inflammatory diseases.. However, it is unclear whether SiO2 exposure-induced pneumonia is attributed to the recruitment and differentiation of pathogenic Th17 cells, and its specific mechanism.

    Article Title: The calcitonin receptor controls osteophyte formation, but not cartilage degeneration and subchondral bone loss in experimental osteoarthritis
    Article Snippet: Quality and quantity of RNA were determined by spectrophotometry NanoDrop 2000 (Thermo Fisher Scientific, USA), and only samples with A260/280 absorbance ratios between 1.9 and 2.1 were considered for complementary DNA (cDNA) synthesis. .. A total of 1 μg of the extracted RNA was reverse transcribed to cDNA using a ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs, USA), following manufacturer’s instructions. .. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed in a final reaction of 20 μl, consisting of 3 μl of cDNA (10-fold diluted), 10 μl of SYBR Select Master Mix for CFX (Applied Biosystems, USA), and 1 μl of primers.

    cDNA Synthesis:

    Article Title: Spectrin coordinates cell shape and signaling essential for epidermal differentiation
    Article Snippet: Formation of TER was measured over time using an automated cell monitoring system (cellZscope, nanoAnalytics). .. RNA was extracted from samples using a Direct-zol RNA extraction kit (R2060; Zymo Research), and equal amounts of RNA were reverse transcribed using the ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs). .. Semiquantitative PCR was conducted using a StepOnePlus System (Thermo Fisher Scientific).

    Article Title: Panx3 deficiency in mice impairs bone fracture healing and causes transient hypoglycemia in neonatal animals
    Article Snippet: RNA concentration and quality were determined using a Nanodrop 2000 spectrophotometer (Nanodrop Technology, Wilmington, MA, USA). .. Complementary DNA (cDNA) was prepared using the ProtoScript First Strand cDNA Synthesis Kit (#E6300L, New England BioLabs Inc., Ipswich, MA, USA). .. Quantitative polymerase chain reaction (RT-qPCR) was performed using primers designed for SYBR Select Master Mix for CFX (#4,472,942, Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, USA) or pre-designed probes with the TaqMan Gene Expression Master Mix (#4,369,016, Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, USA).

    Article Title: Japanese soil-borne wheat mosaic virus CP-RT protein associates with the endoplasmic reticulum network and mobile particles at the ER-Golgi interface during infection.
    Article Snippet: 100 mg leaf material ground in liquid nitrogen using a TissueLyser (Qiagen) was processed with the RNeasy Plant Mini kit (Qiagen). .. Alternatively, RNA was extracted from roots and leaves of infected barley plants using NucleoZol (Macherey-Nagel). cDNA was synthesized using random primers and reverse transcriptase (ProtoScript First Strand cDNA Synthesis Kit, NEB). ..

    Article Title: DtxR is a global iron-dependent regulatory protein with multiple roles in the control of gene expression in Corynebacterium diphtheriae .
    Article Snippet: .. RNA-seq raw data can be found at the NCBI SRA database: ID- PRJNA1336240. cDNA synthesis and qPCR The ProtoScript II First-Strand cDNA synthesis kit (New England Biolabs, Inc.) was used to synthesize cDNA from 100 ng of total RNA. qPCR was performed using the ViiA7 (Thermo Fisher Scientific) with Luna Universal qPCR master mix (New England Biolabs, Inc.). ..

    Article Title: SiO₂ exposure triggers NOX1/ROS-dependent epithelial necroptosis and drives Th17 cell activation to initiate pneumonia in mice.
    Article Snippet: SiO2 (silicon dioxide) particles have been classified as one of the most hazardous environmental pollutants for the respiratory system, yet their hazards are often overlooked.. The abnormal activation of Th17 helper T cells that produce interleukin-17 (IL-17) is associated with the development of multiple inflammatory diseases.. However, it is unclear whether SiO2 exposure-induced pneumonia is attributed to the recruitment and differentiation of pathogenic Th17 cells, and its specific mechanism.

    Article Title: Panx3 deficiency in mice impairs bone fracture healing and causes transient hypoglycemia in neonatal animals.
    Article Snippet: RNA concentration and quality were determined using a Nanodrop 2000 spectrophotometer (Nanodrop Technology, Wilmington, MA, USA). .. Complementary DNA (cDNA) was prepared using the ProtoScript First Strand cDNA Synthesis Kit (#E6300L, New England BioLabs Inc., Ipswich, MA, USA). .. Quantitative polymerase chain reaction (RT-qPCR) was performed using primers designed for SYBR Select Master Mix for CFX (#4472942, Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, USA) or pre-designed probes with the TaqMan Gene Expression Master Mix (#4369016, Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, USA).

    Article Title: DtxR is a global iron-dependent regulatory protein with multiple roles in the control of gene expression in Corynebacterium diphtheriae
    Article Snippet: .. The ProtoScript II First-Strand cDNA synthesis kit (New England Biolabs, Inc.) was used to synthesize cDNA from 100 ng of total RNA. .. qPCR was performed using the ViiA7 (Thermo Fisher Scientific) with Luna Universal qPCR master mix (New England Biolabs, Inc.).

    Article Title: The calcitonin receptor controls osteophyte formation, but not cartilage degeneration and subchondral bone loss in experimental osteoarthritis
    Article Snippet: Quality and quantity of RNA were determined by spectrophotometry NanoDrop 2000 (Thermo Fisher Scientific, USA), and only samples with A260/280 absorbance ratios between 1.9 and 2.1 were considered for complementary DNA (cDNA) synthesis. .. A total of 1 μg of the extracted RNA was reverse transcribed to cDNA using a ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs, USA), following manufacturer’s instructions. .. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed in a final reaction of 20 μl, consisting of 3 μl of cDNA (10-fold diluted), 10 μl of SYBR Select Master Mix for CFX (Applied Biosystems, USA), and 1 μl of primers.

    Infection:

    Article Title: Japanese soil-borne wheat mosaic virus CP-RT protein associates with the endoplasmic reticulum network and mobile particles at the ER-Golgi interface during infection.
    Article Snippet: 100 mg leaf material ground in liquid nitrogen using a TissueLyser (Qiagen) was processed with the RNeasy Plant Mini kit (Qiagen). .. Alternatively, RNA was extracted from roots and leaves of infected barley plants using NucleoZol (Macherey-Nagel). cDNA was synthesized using random primers and reverse transcriptase (ProtoScript First Strand cDNA Synthesis Kit, NEB). ..

    Synthesized:

    Article Title: Japanese soil-borne wheat mosaic virus CP-RT protein associates with the endoplasmic reticulum network and mobile particles at the ER-Golgi interface during infection.
    Article Snippet: 100 mg leaf material ground in liquid nitrogen using a TissueLyser (Qiagen) was processed with the RNeasy Plant Mini kit (Qiagen). .. Alternatively, RNA was extracted from roots and leaves of infected barley plants using NucleoZol (Macherey-Nagel). cDNA was synthesized using random primers and reverse transcriptase (ProtoScript First Strand cDNA Synthesis Kit, NEB). ..

    RNA Sequencing:

    Article Title: DtxR is a global iron-dependent regulatory protein with multiple roles in the control of gene expression in Corynebacterium diphtheriae .
    Article Snippet: .. RNA-seq raw data can be found at the NCBI SRA database: ID- PRJNA1336240. cDNA synthesis and qPCR The ProtoScript II First-Strand cDNA synthesis kit (New England Biolabs, Inc.) was used to synthesize cDNA from 100 ng of total RNA. qPCR was performed using the ViiA7 (Thermo Fisher Scientific) with Luna Universal qPCR master mix (New England Biolabs, Inc.). ..

    Real-time Polymerase Chain Reaction:

    Article Title: DtxR is a global iron-dependent regulatory protein with multiple roles in the control of gene expression in Corynebacterium diphtheriae .
    Article Snippet: .. RNA-seq raw data can be found at the NCBI SRA database: ID- PRJNA1336240. cDNA synthesis and qPCR The ProtoScript II First-Strand cDNA synthesis kit (New England Biolabs, Inc.) was used to synthesize cDNA from 100 ng of total RNA. qPCR was performed using the ViiA7 (Thermo Fisher Scientific) with Luna Universal qPCR master mix (New England Biolabs, Inc.). ..

    Article Title: SiO₂ exposure triggers NOX1/ROS-dependent epithelial necroptosis and drives Th17 cell activation to initiate pneumonia in mice.
    Article Snippet: SiO2 (silicon dioxide) particles have been classified as one of the most hazardous environmental pollutants for the respiratory system, yet their hazards are often overlooked.. The abnormal activation of Th17 helper T cells that produce interleukin-17 (IL-17) is associated with the development of multiple inflammatory diseases.. However, it is unclear whether SiO2 exposure-induced pneumonia is attributed to the recruitment and differentiation of pathogenic Th17 cells, and its specific mechanism.

    Quantitative RT-PCR:

    Article Title: SiO₂ exposure triggers NOX1/ROS-dependent epithelial necroptosis and drives Th17 cell activation to initiate pneumonia in mice.
    Article Snippet: SiO2 (silicon dioxide) particles have been classified as one of the most hazardous environmental pollutants for the respiratory system, yet their hazards are often overlooked.. The abnormal activation of Th17 helper T cells that produce interleukin-17 (IL-17) is associated with the development of multiple inflammatory diseases.. However, it is unclear whether SiO2 exposure-induced pneumonia is attributed to the recruitment and differentiation of pathogenic Th17 cells, and its specific mechanism.

    SYBR Green Assay:

    Article Title: SiO₂ exposure triggers NOX1/ROS-dependent epithelial necroptosis and drives Th17 cell activation to initiate pneumonia in mice.
    Article Snippet: SiO2 (silicon dioxide) particles have been classified as one of the most hazardous environmental pollutants for the respiratory system, yet their hazards are often overlooked.. The abnormal activation of Th17 helper T cells that produce interleukin-17 (IL-17) is associated with the development of multiple inflammatory diseases.. However, it is unclear whether SiO2 exposure-induced pneumonia is attributed to the recruitment and differentiation of pathogenic Th17 cells, and its specific mechanism.



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