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cdna  (New England Biolabs)


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    Structured Review

    New England Biolabs cdna
    Cdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+ii+rt+reaction+buffer/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pmc08329733-252-11-22
    Average 99 stars, based on 1832 article reviews
    cdna - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
    Article Snippet: .. For the reverse transcription, 1 μg of DNase treated RNA was transcribed to cDNA using the Protoscript II First Strand cDNA Synthesis Kit (NEB, #E6560). .. Quantitative RT-PCR was performed with the KAPA SYBR Fast qPCR Mix (Roche, #KK4601) and a 1:80 dilution of the cDNA.

    Article Title: High resistance barrier and prophylactic protection in preclinical models of SARS-CoV-2 with two siRNA combination
    Article Snippet: .. Reverse transcription reactions were performed with 6 μl of purified RNA and random hexamer primers using the NEB ProtoScript II First Strand cDNA Synthesis kit. .. The resulting cDNA was used as a template for PCR amplification of the siRNA binding sites, two primer pairs per site, using KapaBiosystems polymerase (KAPA HiFi HotStart ReadyMix) with primers, 5′-acgctgcttctggtaatc-3′ plus 5′-agaaacccttagacacagc-3′, and 5′-ctagataaacgcactacgtg-3′ and 5′-cctgagcaaagaagaagtg-3′ for the COV-siRNA1 binding site, and 5′-agatgccttcaaactcaac-3′ plus 5′-ttctactctgagttgttgc-3′, and 5′-ttggtggcaaaccttgtatc-3′ plus 5′-tgtgtaactggacacattg-3′ for the COV-siRNA2 binding site.

    Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
    Article Snippet: The sample concentration was quantified with the Nanodrop and 50 ng of the linker-ligated sample was used for rRNA depletion using the Ribo-Seq riboPOOL h/m/r depletion kit (siTOOLs, dp-K024-000050). .. The footprints were annealed with RT primer (5’-pRNAGATCGGAAGAGCGTCGTGTAGGGAAAGAG/iSp18/GTGACTGGAGTTCAGAC GTGTGCTC-3’) at 65°C for 5 minutes and subsequently reverse transcribed for 30 minutes at 50°C in 1x Protoscript II Buffer containing 0.5 mM dNTPs, 10 mM DTT, 20 U Superase In and 200 U Protoscript II (NEB, #E6560S). ..

    Article Title: Expression of the IGF‑1Ea isoform in human placentas from third trimester normal and idiopathic intrauterine growth restriction singleton pregnancies: Correlations with clinical and histopathological parameters.
    Article Snippet: intrauterine growth restriction (iuGr) is the second most common obstetric complication after preterm labor. appropriate trophoblast differentiation and placental structure, growth and function are key for the maintenance of pregnancy and normal fetal growth, development and survival. extravillous trophoblast cell proliferation, migra‐ tion and invasion are regulated by molecules produced by the fetomaternal interface, including autocrine factors produced by the trophoblast, such as insulin‐like growth factor (iGF)‐1.. The aim of the present study was to investigate expression patterns of iGF‐1ea isoform in iuGr placenta compared with appropriate for gestational age (aGa) pregnancies.. Placental frozen tissues were collected from 13 aGa and 15 iuGr third trimester pregnancies for detection of iGF‐1ea mrna expression using reverse transcription‐quantitative Pcr.

    cDNA Synthesis:

    Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
    Article Snippet: .. For the reverse transcription, 1 μg of DNase treated RNA was transcribed to cDNA using the Protoscript II First Strand cDNA Synthesis Kit (NEB, #E6560). .. Quantitative RT-PCR was performed with the KAPA SYBR Fast qPCR Mix (Roche, #KK4601) and a 1:80 dilution of the cDNA.

    Article Title: Rapid and quantitative functional interrogation of human enhancer variant activity in live mice
    Article Snippet: After fluorescent-activated cell sorting, 10 μL of RNAprotect (Qiagen, #76104) was immediately added to cells. .. RNA was isolated using the RNeasy Mini Kit (Qiagen, #74104) according to manufacturer instructions. cDNA libraries were constructed from isolated RNA with the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, #E6560) using Oligo(d)T primers and following the manufacturer’s recommended protocol. ..

    Article Title: High resistance barrier and prophylactic protection in preclinical models of SARS-CoV-2 with two siRNA combination
    Article Snippet: .. Reverse transcription reactions were performed with 6 μl of purified RNA and random hexamer primers using the NEB ProtoScript II First Strand cDNA Synthesis kit. .. The resulting cDNA was used as a template for PCR amplification of the siRNA binding sites, two primer pairs per site, using KapaBiosystems polymerase (KAPA HiFi HotStart ReadyMix) with primers, 5′-acgctgcttctggtaatc-3′ plus 5′-agaaacccttagacacagc-3′, and 5′-ctagataaacgcactacgtg-3′ and 5′-cctgagcaaagaagaagtg-3′ for the COV-siRNA1 binding site, and 5′-agatgccttcaaactcaac-3′ plus 5′-ttctactctgagttgttgc-3′, and 5′-ttggtggcaaaccttgtatc-3′ plus 5′-tgtgtaactggacacattg-3′ for the COV-siRNA2 binding site.

    Article Title: Expression of the IGF‑1Ea isoform in human placentas from third trimester normal and idiopathic intrauterine growth restriction singleton pregnancies: Correlations with clinical and histopathological parameters.
    Article Snippet: intrauterine growth restriction (iuGr) is the second most common obstetric complication after preterm labor. appropriate trophoblast differentiation and placental structure, growth and function are key for the maintenance of pregnancy and normal fetal growth, development and survival. extravillous trophoblast cell proliferation, migra‐ tion and invasion are regulated by molecules produced by the fetomaternal interface, including autocrine factors produced by the trophoblast, such as insulin‐like growth factor (iGF)‐1.. The aim of the present study was to investigate expression patterns of iGF‐1ea isoform in iuGr placenta compared with appropriate for gestational age (aGa) pregnancies.. Placental frozen tissues were collected from 13 aGa and 15 iuGr third trimester pregnancies for detection of iGF‐1ea mrna expression using reverse transcription‐quantitative Pcr.

    Isolation:

    Article Title: Rapid and quantitative functional interrogation of human enhancer variant activity in live mice
    Article Snippet: After fluorescent-activated cell sorting, 10 μL of RNAprotect (Qiagen, #76104) was immediately added to cells. .. RNA was isolated using the RNeasy Mini Kit (Qiagen, #74104) according to manufacturer instructions. cDNA libraries were constructed from isolated RNA with the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, #E6560) using Oligo(d)T primers and following the manufacturer’s recommended protocol. ..

    Construct:

    Article Title: Rapid and quantitative functional interrogation of human enhancer variant activity in live mice
    Article Snippet: After fluorescent-activated cell sorting, 10 μL of RNAprotect (Qiagen, #76104) was immediately added to cells. .. RNA was isolated using the RNeasy Mini Kit (Qiagen, #74104) according to manufacturer instructions. cDNA libraries were constructed from isolated RNA with the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, #E6560) using Oligo(d)T primers and following the manufacturer’s recommended protocol. ..

    Purification:

    Article Title: High resistance barrier and prophylactic protection in preclinical models of SARS-CoV-2 with two siRNA combination
    Article Snippet: .. Reverse transcription reactions were performed with 6 μl of purified RNA and random hexamer primers using the NEB ProtoScript II First Strand cDNA Synthesis kit. .. The resulting cDNA was used as a template for PCR amplification of the siRNA binding sites, two primer pairs per site, using KapaBiosystems polymerase (KAPA HiFi HotStart ReadyMix) with primers, 5′-acgctgcttctggtaatc-3′ plus 5′-agaaacccttagacacagc-3′, and 5′-ctagataaacgcactacgtg-3′ and 5′-cctgagcaaagaagaagtg-3′ for the COV-siRNA1 binding site, and 5′-agatgccttcaaactcaac-3′ plus 5′-ttctactctgagttgttgc-3′, and 5′-ttggtggcaaaccttgtatc-3′ plus 5′-tgtgtaactggacacattg-3′ for the COV-siRNA2 binding site.

    Random Hexamer:

    Article Title: High resistance barrier and prophylactic protection in preclinical models of SARS-CoV-2 with two siRNA combination
    Article Snippet: .. Reverse transcription reactions were performed with 6 μl of purified RNA and random hexamer primers using the NEB ProtoScript II First Strand cDNA Synthesis kit. .. The resulting cDNA was used as a template for PCR amplification of the siRNA binding sites, two primer pairs per site, using KapaBiosystems polymerase (KAPA HiFi HotStart ReadyMix) with primers, 5′-acgctgcttctggtaatc-3′ plus 5′-agaaacccttagacacagc-3′, and 5′-ctagataaacgcactacgtg-3′ and 5′-cctgagcaaagaagaagtg-3′ for the COV-siRNA1 binding site, and 5′-agatgccttcaaactcaac-3′ plus 5′-ttctactctgagttgttgc-3′, and 5′-ttggtggcaaaccttgtatc-3′ plus 5′-tgtgtaactggacacattg-3′ for the COV-siRNA2 binding site.



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