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dnase i reaction buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dnase i reaction buffer
    Dnase I Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+ii+reverse+transcriptase+reaction+buffer/Deoxyribonuclease+I/us09790263-1597-7-38
    Average 99 stars, based on 1 article reviews
    dnase i reaction buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Engineering Clostridium thermocellum for production of 2,3-butanediol from cellulose
    Article Snippet: .. Cells were lysed by the addition of 1 μL of Ready-Lyse lysozyme solution (Epicenter, WI, USA) and incubated at room temperature for 20 min. Then, 1 μL of DNase I solution (Thermo Scientific, MA, USA) was added, and lysate was incubated for an additional 20 min at room temperature. ..

    Article Title: Operating CRISPR/Cas12a in a complex nucleic acid sequence background
    Article Snippet: Total RNA was isolated the following day using the Quick-RNA Miniprep Plus Kit (Zymo Research) according to the manufacturer’s instructions, including the on-column DNase I digestion step. .. To ensure complete removal of residual genomic DNA, the eluted RNA was subjected to an additional DNase treatment using the TURBO DNA-freeTM Kit (Invitrogen) under rigorous conditions according to the manufacturer's instructions with an incubation time of 1 h. Complementary DNA (cDNA) synthesis was performed with 100 ng of RNA per sample using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions for qPCR use. qPCR was performed using the Luna® Universal qPCR Kit according to the manufacturer’s protocol. ..

    Reverse Transcription:

    Article Title: Sangyod rice extract attenuates oleic acid–induced hepatic steatosis by modulating apoptotic, inflammatory, and lipid metabolic pathways
    Article Snippet: .. One microgram of total RNA was treated with DNase I (Invitrogen, Thermo Fisher Scientific, USA) to remove genomic DNA contamination and subsequently reverse-transcribed into first-strand cDNA at 42 °C for 1 h using the SuperScriptTM VILO cDNA Synthesis Kit (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA). .. Quantitative real-time PCR (qPCR) was performed using the SensiFASTTM SYBR® No-ROX Kit (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) in a real-time PCR detection system (BIOER, Hangzhou Bioer Technology Co., Ltd., Hangzhou, China).

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis
    Article Snippet: RNA concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo ScientificTM). .. For cDNA synthesis, 1 μg of total RNA was treated with DNase I (Invitrogen) and reverse-transcribed using the HiScript® III RT SuperMix (+gDNA wiper) kit (R323-01, Vazyme, Nanjing, China). ..

    cDNA Synthesis:

    Article Title: Sangyod rice extract attenuates oleic acid–induced hepatic steatosis by modulating apoptotic, inflammatory, and lipid metabolic pathways
    Article Snippet: .. One microgram of total RNA was treated with DNase I (Invitrogen, Thermo Fisher Scientific, USA) to remove genomic DNA contamination and subsequently reverse-transcribed into first-strand cDNA at 42 °C for 1 h using the SuperScriptTM VILO cDNA Synthesis Kit (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA). .. Quantitative real-time PCR (qPCR) was performed using the SensiFASTTM SYBR® No-ROX Kit (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) in a real-time PCR detection system (BIOER, Hangzhou Bioer Technology Co., Ltd., Hangzhou, China).

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis
    Article Snippet: RNA concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo ScientificTM). .. For cDNA synthesis, 1 μg of total RNA was treated with DNase I (Invitrogen) and reverse-transcribed using the HiScript® III RT SuperMix (+gDNA wiper) kit (R323-01, Vazyme, Nanjing, China). ..

    Article Title: Operating CRISPR/Cas12a in a complex nucleic acid sequence background
    Article Snippet: Total RNA was isolated the following day using the Quick-RNA Miniprep Plus Kit (Zymo Research) according to the manufacturer’s instructions, including the on-column DNase I digestion step. .. To ensure complete removal of residual genomic DNA, the eluted RNA was subjected to an additional DNase treatment using the TURBO DNA-freeTM Kit (Invitrogen) under rigorous conditions according to the manufacturer's instructions with an incubation time of 1 h. Complementary DNA (cDNA) synthesis was performed with 100 ng of RNA per sample using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions for qPCR use. qPCR was performed using the Luna® Universal qPCR Kit according to the manufacturer’s protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Operating CRISPR/Cas12a in a complex nucleic acid sequence background
    Article Snippet: Total RNA was isolated the following day using the Quick-RNA Miniprep Plus Kit (Zymo Research) according to the manufacturer’s instructions, including the on-column DNase I digestion step. .. To ensure complete removal of residual genomic DNA, the eluted RNA was subjected to an additional DNase treatment using the TURBO DNA-freeTM Kit (Invitrogen) under rigorous conditions according to the manufacturer's instructions with an incubation time of 1 h. Complementary DNA (cDNA) synthesis was performed with 100 ng of RNA per sample using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions for qPCR use. qPCR was performed using the Luna® Universal qPCR Kit according to the manufacturer’s protocol. ..

    Concentration Assay:

    Article Title: Stage-dependent effects of composite probiotics on gut mineral transport, Skeletal health, and egg quality in laying hens
    Article Snippet: Following the manufacturer's instructions, total RNA was isolated from intestinal and tibial bone tissues with TRIzol reagent (Takara, Dalian, China). .. To eliminate potential genomic DNA contamination, the extracted RNA was subsequently treated with DNase I. RNA concentration and purity were assessed spectrophotometrically using a Nanodrop instrument, with all samples exhibiting A260/A280 ratio between 1.8 and 2.1. ..



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