total rna isolation nucleospin rna ii kit (MACHEREY NAGEL)

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MACHEREY NAGEL total rna isolation nucleospin rna ii kit
Total Rna Isolation Nucleospin Rna Ii Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 13848 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protoscript+ii+reverse+transcriptase+reaction+buffer/NucleoSpin+RNA+%2F+Protein%2C+Mini+kit+for+RNA+and+protein+purification/pm26143143-97-17-24
Average 96 stars, based on 13848 article reviews

total rna isolation nucleospin rna ii kit - by Bioz Stars, 2026-10

96/100 stars

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Isolation:

Article Title: Peripartum-Associated Heart Failure Develops Independently of RHOT Proteins.
Article Snippet: Citrate synthase and HADH activities in LV tissue were measured using a SynergyTM HT multi-detection microplate reader (BioTek Instruments, Winooski, VT, USA) in a total reaction volume of 200 μL, as previously described [45]. .. Total RNA from LV tissue was isolated using the NucleoSpin RNA kit (MachereyNagel, Düren, Germany). .. RNA quality and integrity were assessed using a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany).

Article Title: Biallelic rescue of CTG18.1 in two Fuchs endothelial corneal dystrophy-derived iPSC lines (SCTCi047-A-2, SCTCi046-A-2) following a two-step gene editing strategy.
Article Snippet: .. RT-qPCR Total RNA was isolated from the hiPSCs with NucleoSpin RNA Mini Kit (Macherey-Nagel), according to the manufacturer’s instructions. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad). .. RTqPCR was performed (Table 2) with GoTaq qPCR Master Mix List of recommended germ layer markers qRT-PCR Endoderm (SOX17 and CXCR4), mesoderm (TBXT and HAND1), ectoderm (PAX6 and OTX2) Fig. 1F Outcomes of gene editing experiment (OPTIONAL) N/A N/A N/A Donor screening (OPTIONAL) HIV-1 and − 2, Hepatitis B, Hepatitis C N/A N/A Genotype − additional histocompatibility info (OPTIONAL) Blood group genotyping N/A N/A HLA tissue typing N/A N/A (Promega) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific).

Article Title: Gene loss and compensatory evolution as a source of metabolic pre-adaptations.
Article Snippet: .. Total RNA was isolated with Nucleospin RNA Kit (Macherey Nagel) 18 following the manufacturer’s instruction. ..

Article Title: Thapsigargin-induced autophagic flux impairment and inflammation are potentiated by CLN3 deficiency and alleviated by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) in human ARPE-19 cells.
Article Snippet: .. RNA was isolated by lysing the cells directly on a cell culture plate 48 h after siRNA transfections using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's instructions. .. A Nanodrop (NanoDrop Technologies, Model: ND-1000) spectrophotometer was used to measure RNA purity and concentration at the wavelength of 260/280 nm. cDNA synthesis was performed using the SuperScript® VILOTM cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, US) according to the manufacturer's instructions.

Control:

Article Title: Modelling 16/8 intermittent fasting and breakfast-skipping in mouse adipocytes 3T3-L1 in vitro: a transcriptomics study.
Article Snippet: .. Total RNA was extracted from treated 3T3-L1 adipocytes of the control group, 16/8 IF group (most effective treatment), and breakfastskipping group (least effective treatment) using the NucleoSpin® RNA extraction kit (MACHEREY-NAGEL, Germany) following the manufacturer’s protocol with slight modifications. ..

RNA Extraction:

Protein Purification:

Article Title: SRF3 couples cellular iron homeostasis and defense responses through ubiquitin-dependent nano-organization.
Article Snippet: .. Total RNA was extracted from roots of plants 5 days after germination using an RNA protein purification kit (Macherey- Nagel). .. Nextgeneration sequencing libraries were generated using the TruSeq Stranded mRNA library prep kits (Illumina, San Diego, CA, USA) and sequenced on a HiSeq 2500 instrument as single- end 50 base reads.

Purification:

Article Title: Sustained antigen-specific CD8+ T cell immunity post-mRNA booster requires notch pathway activation.
Article Snippet: .. Total RNA was purified using a NucleoSpin RNA Plus XS kit (MACHEREY-NAGEL) according to the manufacturer’s protocol. cDNA was synthesized from total RNA using SuperScript III First-Strand Synthesis system with oligo(dT) (Thermo Fisher Scientific), following the manufacturer’s protocol. .. Quantitative polymerase chain reaction was performed using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a StepOne plus (Thermo Fisher Scientific).

Synthesized:

Quantitative RT-PCR:

cDNA Synthesis:

Extraction:

Article Title: Toxicogenomic signatures in zebrafish embryos provide a resource for mode-of-action-informed ecotoxic hazard screening.
Article Snippet: .. At 96 hpf, the experiment was terminated by extracting RNA using the NucleoSpin RNA/protein extraction kit (Macherey-Nagel, Düren, Germany). .. For this, ten embryos were randomly collected from each Petri dish and euthanized on ice for 10 min, before being homogenised in lysis buffer provided by the manufacturer (Macherey-Nagel, Düren, Germany) using lysing matrix ceramic beads at a speed of 5 m/s for 45 s in a FastPrep-24 homogeniser (MP Biomedicals, Irvine, CA, USA).

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