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Becton Dickinson 1x lysing buffer
1x Lysing Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protoscript+ii+buffer+1x/1%C3%97+lyse+buffer/pmc09718471-323-7-10
Average 90 stars, based on 1 article reviews
1x lysing buffer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Lysis:

Article Title: Preclinical Efficacy of VTX-0811: A Humanized First-in-Class PSGL-1 mAb Targeting TAMs to Suppress Tumor Growth.
Article Snippet: After centrifugation, 100 μL of a secondary antibody (Invitrogen, Waltham, MA, USA, Cat. #A55749; diluted 1:10) was added per well, and the cells were incubated for 10 min on ice followed by the addition of 120 μL NWB to each well. .. The cells were centrifuged and washed with 200 μL NWB as above and then resuspended in 200 μL of 1× Lysis buffer (Becton Dickinson, Franklin Lakes, NJ, USA, Cat #349202 1:10 in ddH20) and incubated for 10 min at room temperature. .. After centrifugation, 100 μL of a secondary antibody (Invitrogen, Waltham, MA, USA, Cat. #A55749; diluted 1:10) was added per well, and the cells were incubated for 10 min on ice followed by the addition of 120 μL NWB to each well.

Article Title: Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance
Article Snippet: .. The resuspended live single cells were stained separately using a LIVE/DEAD Fixable Violet Dead Cell Staining Kit and blocked using TruStain FcXTM after the red blood cells were removed using 1× lysis buffer (BD biosciences 555,899). ..

Article Title: Lymphostatin, a virulence factor of attaching and effacing Escherichia coli , inhibits proliferation and cytokine responses of human T cells in a manner associated with cell cycle arrest but not apoptosis or necrosis
Article Snippet: .. To lyse red blood cells, the cell pellet was resuspended in 1 ml of 1X lysing buffer (BD Biosciences) and incubated for 5 - 10 min. To stop cell lysis, 2% (v/v) FBS in RPMI medium was then added. ..

Incubation:

Article Title: Preclinical Efficacy of VTX-0811: A Humanized First-in-Class PSGL-1 mAb Targeting TAMs to Suppress Tumor Growth.
Article Snippet: After centrifugation, 100 μL of a secondary antibody (Invitrogen, Waltham, MA, USA, Cat. #A55749; diluted 1:10) was added per well, and the cells were incubated for 10 min on ice followed by the addition of 120 μL NWB to each well. .. The cells were centrifuged and washed with 200 μL NWB as above and then resuspended in 200 μL of 1× Lysis buffer (Becton Dickinson, Franklin Lakes, NJ, USA, Cat #349202 1:10 in ddH20) and incubated for 10 min at room temperature. .. After centrifugation, 100 μL of a secondary antibody (Invitrogen, Waltham, MA, USA, Cat. #A55749; diluted 1:10) was added per well, and the cells were incubated for 10 min on ice followed by the addition of 120 μL NWB to each well.

Article Title: Lymphostatin, a virulence factor of attaching and effacing Escherichia coli , inhibits proliferation and cytokine responses of human T cells in a manner associated with cell cycle arrest but not apoptosis or necrosis
Article Snippet: .. To lyse red blood cells, the cell pellet was resuspended in 1 ml of 1X lysing buffer (BD Biosciences) and incubated for 5 - 10 min. To stop cell lysis, 2% (v/v) FBS in RPMI medium was then added. ..

other:

Article Title: LPS differentially affects expression of CD14 and CCR2 in monocyte subsets of Post-STEMI patients with hyperglycemia.
Article Snippet: Aims: Following ST-segment elevation myocardial infarction (STEMI), recruitment and activation of monocytes [classical (CD14++CD16-CCR2++), intermediate (CD14CD16CCR2), non-classical (CD14LowCD16++CCR2Low)] are needed for myocardial wound healing.. Monocyte surface receptor C–C chemokine receptor type 2 (CCR2) is responsible for monocyte chemotaxis to sites of inflammation and the lipopolysaccharide (LPS)binding protein co-receptor, CD14, is involved in pro-inflammatory monocyte activation.. The purpose of this investigation was to determine the effects of ex-vivo LPS activation on monocyte subset CD14 and CCR2 expression in post-STEMI individuals with normal and elevated random blood glucose.

Article Title: Excessive neutrophil recruitment promotes typical T-helper 17 responses in Coronavirus disease 2019 patients
Article Snippet: The 1X lysing solution was prepared by diluting the 10X concentrate of the BD MultitestTM IMK kit lysing solution with deionised water.

Staining:

Article Title: Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance
Article Snippet: .. The resuspended live single cells were stained separately using a LIVE/DEAD Fixable Violet Dead Cell Staining Kit and blocked using TruStain FcXTM after the red blood cells were removed using 1× lysis buffer (BD biosciences 555,899). ..



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