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Servicebio Inc sds page protein loading buffer
Sds Page Protein Loading Buffer, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+loading+buffer/buffer+loading/pmc13265561-92-8-12
Average 86 stars, based on 1 article reviews
sds page protein loading buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

SDS Page:

Article Title: Nanozyme-engineered liners for proactive prevention of wear particle-induced osteolysis.
Article Snippet: .. Protein extracts were mixed with 5× SDS-PAGE loading buffer (Servicebio #G2075) and heated at 100 °C for 5 min before storage. ..

Article Title: Identification and functional characterization of plasma exosomal miR-618 as a novel biomarker in recurrent depressive disorder
Article Snippet: The supernatant contained total protein who’s concentration was determined using the BCA Protein Concentration Assay Kit (LABLEAD, China). .. The remaining protein samples were mixed with 5× SDS-PAGE protein loading buffer (Servicebio, China) at a 4:1 (v/v) ratio and heat-denatured at 95 °C for 5 min using a metal heating block. .. 40 μg of protein was separated on a 10% SDS-PAGE gel (pre-cast using a One Step PAGE Gel Super Fast Preparation Kit, Meilun, China) by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under constant voltage (200 V) for 45 min, followed by being electrophoretically transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon, Germany) at 400 mA for 25 min. After incubation for 1 h at room temperature with Tris-Buffered Saline with Tween 20 (TBST, Servicebio, China) containing 5% Bovine Serum Albumin (Beyotime, China), the PVDF membrane was rinsed three times with TBST for 10 min each, followed by overnight incubation at 4 °C with the following primary antibody: CD9 Recombinant Rabbit Monoclonal Antibody (clone NO. SA35-08, Cat# ET1601-9, 1:1,000, HUABIO, China), CD63 Recombinant Rabbit Monoclonal Antibody (clone NO. SY21-02, Cat# ET1607-2, 1:500, HUABIO, China) and TSG101 Recombinant Rabbit Monoclonal Antibody (clone NO. JJ0900, Cat# ET1701-59, 1:2000, HUABIO, China).

Article Title: A mechanism of target mRNA selection and activity regulation in meiosis-related RBM46-MEIOC-YTHDC2 complex
Article Snippet: PAGE Gel Rapid Preparation Kit , EPIZYME , Cat#PG111. .. SDS-PAGE running buffer powder , Servicebio , Cat#G2018-1L. .. Rapid Membrane Transfer Buffer , EPIZYME , Cat#PS109S.

Article Title: High expression of the underexplored SLC4A11 protein-coding transcript is specific to the corneal endothelium.
Article Snippet: The cells were detached from a 6-well plate using 0.25% w/v Trypsin-EDTA (Thermo Fisher Scientific; Waltham, Massachusetts, USA). .. Lysates were AR TIC LE IN PR ES S prepared using 2x SDS-PAGE Loading Buffer (Non-Reduced) (G2032-1ML, Servicebio) supplemented with beta-mercaptoethanol to a concentration of 5%. ..

Blocking Assay:

Article Title: Identification and functional characterization of plasma exosomal miR-618 as a novel biomarker in recurrent depressive disorder
Article Snippet: The supernatant contained total protein who’s concentration was determined using the BCA Protein Concentration Assay Kit (LABLEAD, China). .. The remaining protein samples were mixed with 5× SDS-PAGE protein loading buffer (Servicebio, China) at a 4:1 (v/v) ratio and heat-denatured at 95 °C for 5 min using a metal heating block. .. 40 μg of protein was separated on a 10% SDS-PAGE gel (pre-cast using a One Step PAGE Gel Super Fast Preparation Kit, Meilun, China) by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under constant voltage (200 V) for 45 min, followed by being electrophoretically transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon, Germany) at 400 mA for 25 min. After incubation for 1 h at room temperature with Tris-Buffered Saline with Tween 20 (TBST, Servicebio, China) containing 5% Bovine Serum Albumin (Beyotime, China), the PVDF membrane was rinsed three times with TBST for 10 min each, followed by overnight incubation at 4 °C with the following primary antibody: CD9 Recombinant Rabbit Monoclonal Antibody (clone NO. SA35-08, Cat# ET1601-9, 1:1,000, HUABIO, China), CD63 Recombinant Rabbit Monoclonal Antibody (clone NO. SY21-02, Cat# ET1607-2, 1:500, HUABIO, China) and TSG101 Recombinant Rabbit Monoclonal Antibody (clone NO. JJ0900, Cat# ET1701-59, 1:2000, HUABIO, China).

Imaging:

Article Title: Acetylated mitochondrial MDH2 regulates CTR2 transcription to induce cuproptosis during Escherichia coli infection.
Article Snippet: Equal protein aliquots were denatured in 5× SDS-PAGE loading buffer (Yeasen #20315ES) at 95°C for 5 min, separated on 4–12% Bis-Tris gels, and electroblotted onto PVDF membranes. .. Membranes were blocked with protein-free buffer (Servicebio #G2052), probed with primary antibodies (4°C, overnight), and detected using HRPconjugated secondary antibodies (1 h, room temperature) with chemiluminescence imaging (Servicebio SCG-W5000). .. Non-denatured protein samples were solubilized in NativePAGETM buffer (Invitrogen #BN2003), resolved on NativePAGETM Bis-Tris gels (Invitrogen #BN1001BOX), and transferred to PVDF membranes.

Concentration Assay:

Article Title: High expression of the underexplored SLC4A11 protein-coding transcript is specific to the corneal endothelium.
Article Snippet: The cells were detached from a 6-well plate using 0.25% w/v Trypsin-EDTA (Thermo Fisher Scientific; Waltham, Massachusetts, USA). .. Lysates were AR TIC LE IN PR ES S prepared using 2x SDS-PAGE Loading Buffer (Non-Reduced) (G2032-1ML, Servicebio) supplemented with beta-mercaptoethanol to a concentration of 5%. ..



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Image Search Results


Journal: Frontiers in Oncology

Article Title: Integrating network pharmacology, molecular docking, and experimental validation, this study explores the mechanism of action of Typhonii Rhizoma in colon cancer

doi: 10.3389/fonc.2026.1815506

Figure Lengend Snippet: Experiment-related reagents.

Article Snippet: 5× Protein Loading Buffer (DTT-containing) , Epizyme , 4% Hematoxylin and Eosin Fixative , Solarbio.

Techniques: CCK-8 Assay, Protease Inhibitor, Western Blot, Membrane, Electrophoresis