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lamin b1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology lamin b1
    (A) Immunofluorescence of SKmel147 cells stably expressing AMIGO2-GFP (green), stained with AMIGO2 antibody (red) and Hoechst 33342 (blue). Scale bar, 20 μm. (B) Functional annotation of AMIGO2-interacting proteins detected by GFP pull-down followed by MS in SKmel147 cells stably expressing AMIGO2-GFP (see Table S4). (C) PTK7 and GFP immunoblots following GFP pull-down from 501MEL cells stably expressing AMIGO2-GFP. (D) Full-length PTK7 (FL-PTK7), C-terminal fragments CTF1- and CTF2-PTK7, and FOXM1 immunoblots of 501MEL cells 72 hr post-infection with shSCR or shPTK7 (shP7 #1 and #2). Actin was used as a loading control. (E) Relative growth curves of 501MEL (left) and SKmel147 (right) cells stably transduced with shSCR or shPTK7 (shP7 #1 and #2). Values are normalized to seeding control (n = 3). (F) Percent Annexin V-positive cells 6 days post-transduction for same cells as in (E). (G) FL-PTK7, CTF-PTK7, and FOXM1 immunoblots of 501MEL cells 48 hr post-transduction with shSCR or shAMIGO2 (shA2 #1 and #2). Actin was used as a loading control. (H) FL-PTK7, CTF-PTK7, FOXM1, and AMIGO2 immunoblots of 501MEL cells untreated or treated with JQ1 (JQ1[+]) for 72 hr. Tubulin was used as a loading control. (I) CTF2-PTK7 immunoblot of nuclear lysates from same cell as in (G) (left). <t>Lamin</t> <t>B1</t> was used as loading control. Signal quantification (right), normalized to Lamin B1, relative to shSCR (n = 3). All values and error bars represent mean ± SD or ± SEM. See also Figures S3 and S4.
    Lamin B1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+binding+microarray+chips/Lamin+B1+(m)-PR/pmc05993436-16-0-3
    Average 93 stars, based on 32 article reviews
    lamin b1 - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene"

    Article Title: Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2017.11.004

    (A) Immunofluorescence of SKmel147 cells stably expressing AMIGO2-GFP (green), stained with AMIGO2 antibody (red) and Hoechst 33342 (blue). Scale bar, 20 μm. (B) Functional annotation of AMIGO2-interacting proteins detected by GFP pull-down followed by MS in SKmel147 cells stably expressing AMIGO2-GFP (see Table S4). (C) PTK7 and GFP immunoblots following GFP pull-down from 501MEL cells stably expressing AMIGO2-GFP. (D) Full-length PTK7 (FL-PTK7), C-terminal fragments CTF1- and CTF2-PTK7, and FOXM1 immunoblots of 501MEL cells 72 hr post-infection with shSCR or shPTK7 (shP7 #1 and #2). Actin was used as a loading control. (E) Relative growth curves of 501MEL (left) and SKmel147 (right) cells stably transduced with shSCR or shPTK7 (shP7 #1 and #2). Values are normalized to seeding control (n = 3). (F) Percent Annexin V-positive cells 6 days post-transduction for same cells as in (E). (G) FL-PTK7, CTF-PTK7, and FOXM1 immunoblots of 501MEL cells 48 hr post-transduction with shSCR or shAMIGO2 (shA2 #1 and #2). Actin was used as a loading control. (H) FL-PTK7, CTF-PTK7, FOXM1, and AMIGO2 immunoblots of 501MEL cells untreated or treated with JQ1 (JQ1[+]) for 72 hr. Tubulin was used as a loading control. (I) CTF2-PTK7 immunoblot of nuclear lysates from same cell as in (G) (left). Lamin B1 was used as loading control. Signal quantification (right), normalized to Lamin B1, relative to shSCR (n = 3). All values and error bars represent mean ± SD or ± SEM. See also Figures S3 and S4.
    Figure Legend Snippet: (A) Immunofluorescence of SKmel147 cells stably expressing AMIGO2-GFP (green), stained with AMIGO2 antibody (red) and Hoechst 33342 (blue). Scale bar, 20 μm. (B) Functional annotation of AMIGO2-interacting proteins detected by GFP pull-down followed by MS in SKmel147 cells stably expressing AMIGO2-GFP (see Table S4). (C) PTK7 and GFP immunoblots following GFP pull-down from 501MEL cells stably expressing AMIGO2-GFP. (D) Full-length PTK7 (FL-PTK7), C-terminal fragments CTF1- and CTF2-PTK7, and FOXM1 immunoblots of 501MEL cells 72 hr post-infection with shSCR or shPTK7 (shP7 #1 and #2). Actin was used as a loading control. (E) Relative growth curves of 501MEL (left) and SKmel147 (right) cells stably transduced with shSCR or shPTK7 (shP7 #1 and #2). Values are normalized to seeding control (n = 3). (F) Percent Annexin V-positive cells 6 days post-transduction for same cells as in (E). (G) FL-PTK7, CTF-PTK7, and FOXM1 immunoblots of 501MEL cells 48 hr post-transduction with shSCR or shAMIGO2 (shA2 #1 and #2). Actin was used as a loading control. (H) FL-PTK7, CTF-PTK7, FOXM1, and AMIGO2 immunoblots of 501MEL cells untreated or treated with JQ1 (JQ1[+]) for 72 hr. Tubulin was used as a loading control. (I) CTF2-PTK7 immunoblot of nuclear lysates from same cell as in (G) (left). Lamin B1 was used as loading control. Signal quantification (right), normalized to Lamin B1, relative to shSCR (n = 3). All values and error bars represent mean ± SD or ± SEM. See also Figures S3 and S4.

    Techniques Used: Immunofluorescence, Stable Transfection, Expressing, Staining, Functional Assay, Western Blot, Infection, Control, Transduction

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Microarray, Derivative Assay, Recombinant, Magnetic Beads, Flow Cytometry, Caspase Activity Assay, DNA Library Preparation, Blocking Assay, Extraction, TA Cloning, Sequencing, RNA Sequencing, Western Blot, Mass Spectrometry, Expressing, Software

    Related Articles

    other:

    Article Title: The transcription factor PREP1(PKNOX1) regulates nuclear stiffness, the expression of LINC complex proteins and mechanotransduction
    Article Snippet: IF: LAMIN B1 (Goat, 1:400, Santa Cruz Biotechnology), LAMIN A/C (Mouse, 1:1000, Santa Cruz Biotechnology), SUN1 (Rabbit, 1:200, Abcam), SUN2 (Rabbit, 1:200, Abcam), LAP2α (Rabbit, 1:400, Abcam), LAP2β (Mouse, 1:200, Sigma), LBR (Rabbit, 1:400, Abcam), EMERIN (Mouse, 1:500, Leica), PREP1 (Mouse, 1:200, Santa Cruz Biotechnology), vinculin (Mouse, 1:1000, Sigma-Aldrich), YAP-TAZ (Mouse, 1:200, Sigma-Aldrich), Nesprin1 (Mouse, 1:200, ThermoFisher Scientific), Nesprin-2 (Mouse, 1:200, Immuquest).

    Nonspecific Binding:

    Article Title: Functional Expression of Multidrug-Resistance (MDR) Transporters in Developing Human Fetal Brain Endothelial Cells
    Article Snippet: The hfBECs were rinsed with cold PBS, fixed with 4% PFA (Electron Microscopy Sciences) for 15 min and then permeabilized with Triton X-100 (lab grade, Sigma) (0.2%, 5 min, room temperature). .. Autofluorescence was reduced using 0.1% Sudan Black in 70% ethanol (1 min) and non-specific binding was blocked using 1% BSA for 1 h. The hfBECs slides were incubated with primary P-gp, BCRP and vWF antibodies described above, as well as glial fibrillary acidic protein (GFAP), (36705,1:300, Cell Signalling Technology, Danvers, MA, USA) and Lamin B1 (sc-6217,1:200, Santa Cruz Biotechnology, Dallas, TX, USA) overnight at 4 °C. .. For ZO-1 (339100, 1:200, Invitrogen, ON, Canada) immunofluorescence cells were permeabilized with 0.5% Triton X-100 (lab grade, Sigma) for 15 min and blocked using 2% BSA (BioShop) for 1 h. For Claudin5 (4C3C2, 1:500, Invitrogen) immunofluorescence, cells were fixed with methanol for 10 min and permeabilized with 0.1% Triton X-100 (lab grade, Sigma) for 10 min and blocked using 3% BSA (BioShop) for 10 min and incubated with primary overnight at 4 °C.

    Incubation:

    Article Title: Functional Expression of Multidrug-Resistance (MDR) Transporters in Developing Human Fetal Brain Endothelial Cells
    Article Snippet: The hfBECs were rinsed with cold PBS, fixed with 4% PFA (Electron Microscopy Sciences) for 15 min and then permeabilized with Triton X-100 (lab grade, Sigma) (0.2%, 5 min, room temperature). .. Autofluorescence was reduced using 0.1% Sudan Black in 70% ethanol (1 min) and non-specific binding was blocked using 1% BSA for 1 h. The hfBECs slides were incubated with primary P-gp, BCRP and vWF antibodies described above, as well as glial fibrillary acidic protein (GFAP), (36705,1:300, Cell Signalling Technology, Danvers, MA, USA) and Lamin B1 (sc-6217,1:200, Santa Cruz Biotechnology, Dallas, TX, USA) overnight at 4 °C. .. For ZO-1 (339100, 1:200, Invitrogen, ON, Canada) immunofluorescence cells were permeabilized with 0.5% Triton X-100 (lab grade, Sigma) for 15 min and blocked using 2% BSA (BioShop) for 1 h. For Claudin5 (4C3C2, 1:500, Invitrogen) immunofluorescence, cells were fixed with methanol for 10 min and permeabilized with 0.1% Triton X-100 (lab grade, Sigma) for 10 min and blocked using 3% BSA (BioShop) for 10 min and incubated with primary overnight at 4 °C.

    Article Title: Hepatic protective effects of sulforaphane through the modulation of inflammatory pathways.
    Article Snippet: The aim of this study was to investigate the effects of sulforaphane (SFN) on lipopolysaccharide (LPS)-induced liver failure, and to elucidate underlying mechanisms.. SFN, a natural isothiocyanate present in cruciferous vegetables such as broccoli and cabbage, is effective in preventing carcinogenesis, diabetes, and inflammatory responses.. Mice were treated intravenously with SFN at 12h after LPS treatment.

    Western Blot:

    Article Title: YAP and TAZ are distinct effectors of corneal myofibroblast transformation
    Article Snippet: Protein was separated on precast gels (NuPAGE 10% Bis-Tris; Invitrogen) and transferred onto nitrocellulose membranes. .. Immunoblotting was done against anti-human YAP-H9 (sc-271134, Santa Cruz Biotechnologies, Santa Cruz, CA); TAZ-H70 (sc-48805, Santa Cruz Biotechnologies, Santa Cruz, CA); and pan-actin (MAB1501, Millipore), pTAZ (ser 89, sc-17610; Santa Cruz Biotechnologies); pYAP (S127, D9W21; Cell Signalling); pSmad4 (T277, PA5–12695; Thermo Scientific); Smad4 (B-8, sc-7966; Santa Cruz); pSmad3 (S423 + S425, ab52903; Abcam); Smad3 (C67H9, Cell Signalling); pSmad2 (S467, ab53100; Abcam); Smad2 (D43B4, Cell Signalling), αSMA (A5228; Sigma), β-Tubulin (ab15568, abcam), GAPDH (CS207795, Millipore), CTGF (ab6992; Abcam) and Lamin B1 (sc-20682, Santa Cruz Biotechnologies) overnight at 4 °C. .. This was followed by incubation with secondary antibodies conjugated with horseradish peroxidase (HRP; SeraCare) for 1 h at 37 °C.

    Article Title: The Role of Runx2 in Microtubule Acetylation in Bone Metastatic Breast Cancer Cells
    Article Snippet: For all western blots conducted using cytoplasmic and whole-cell lysate, β-actin (CST) was used as a loading control. .. Lamin B1 (Santa Cruz Biotechnology, Dallas, TX, USA) was utilized as a loading control for western blots involving nuclear protein fractions. .. Single-cell clones of virally-transduced BoM-MDA-231 cells expressing Runx2 shRNA were washed with ice-cold PBS, and then cells were lysed in 500 μL of immunoprecipitation buffer (20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM sodium orthovanadate, 1% Triton X-100) supplemented with complete mini EDTA free protease inhibitor tablets (Roche) and 1 mM PMSF at the time of use.

    Control:

    Article Title: The Role of Runx2 in Microtubule Acetylation in Bone Metastatic Breast Cancer Cells
    Article Snippet: For all western blots conducted using cytoplasmic and whole-cell lysate, β-actin (CST) was used as a loading control. .. Lamin B1 (Santa Cruz Biotechnology, Dallas, TX, USA) was utilized as a loading control for western blots involving nuclear protein fractions. .. Single-cell clones of virally-transduced BoM-MDA-231 cells expressing Runx2 shRNA were washed with ice-cold PBS, and then cells were lysed in 500 μL of immunoprecipitation buffer (20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM sodium orthovanadate, 1% Triton X-100) supplemented with complete mini EDTA free protease inhibitor tablets (Roche) and 1 mM PMSF at the time of use.



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