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proprietary blocking buffer  (LI-COR)


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    LI-COR proprietary blocking buffer
    Proprietary Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 2162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proprietary+blocking+buffer/Intercept+(TBS)+Blocking+Buffer/us12415841-461-6-10
    Average 98 stars, based on 2162 article reviews
    proprietary blocking buffer - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Membrane:

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells
    Article Snippet: The proteins from the gel were transferred to a Trans‐Blot Turbo Midi 0.2 μm PVDF Transfer Packs (Bio‐Rad) nitrocellulose membrane using the Mix MW function of the Trans‐Blot Turbo Transfer System. .. The membrane was stained with Ponceau S solution (Sigma–Aldrich) and then blocked with 50% Intercept (TBS) Blocking Buffer (Licor) in TBS (Thermo Fisher Scientific). .. Following blocking, the membrane was incubated overnight at 4°C with primary antibodies in v/v 50%/50% Intercept (TBS) Blocking Buffer (Licor)/TBS (Thermo Fisher Scientific) supplemented with 0.1% Tween‐20 (Sigma–Aldrich).

    Article Title: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness.
    Article Snippet: Immunoblot to detect Cre recombinase expression Cells were lysed in Laemmli buffer, supplemented with protease and phosphatase inhibitors (cOmplete and EDTA-free Protease Inhibitor cocktail; Roche, COEDTAF-RO and PHOSS-RO). .. Proteins were separated in NuPage Bis-Tris gels (Invitrogen, NP0322BOX), transferred on a nitrocellulose membrane (0.2μm; Bio-Rad), and blocked by incubation with Intercept (TBS) Blocking buffer (LI-COR: 927-60001). .. The following primary antibodies were used: anti-Cre recombinase (1:1,000; Cell Signaling, 15036) and anti-α-tubulin (1:5,000; Cell Signaling, 2144).

    Article Title: Transcriptomic Plasticity and Its Spatial Architecture Shape Melanoma Response to Immunotherapy and Its Combination With BRAF/MEKi
    Article Snippet: PURPOSE In BRAF-mutant melanoma, clinical trials evaluating combination and sequencing strategies of MAPK-targeted therapy with immune checkpoint blockade (ICB) have yielded mixed results.. We sought to define tumor biology associated with resistance to ICB and response to ICB and its combination with MAPK-targeted therapy, and determine biomarkers to guide treatment strategies.. PATIENTS AND METHODS We integrated clinical with multi-omics data from IMspire150, BRIM, AVASTM, and publicly available immunotherapy-treated melanoma cohorts to identify transcriptional states associated with response or resistance to ICB and BRAF/MEK inhibition combined with ICB.

    Staining:

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells
    Article Snippet: The proteins from the gel were transferred to a Trans‐Blot Turbo Midi 0.2 μm PVDF Transfer Packs (Bio‐Rad) nitrocellulose membrane using the Mix MW function of the Trans‐Blot Turbo Transfer System. .. The membrane was stained with Ponceau S solution (Sigma–Aldrich) and then blocked with 50% Intercept (TBS) Blocking Buffer (Licor) in TBS (Thermo Fisher Scientific). .. Following blocking, the membrane was incubated overnight at 4°C with primary antibodies in v/v 50%/50% Intercept (TBS) Blocking Buffer (Licor)/TBS (Thermo Fisher Scientific) supplemented with 0.1% Tween‐20 (Sigma–Aldrich).

    Transcranial Magnetic Stimulation:

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells
    Article Snippet: The proteins from the gel were transferred to a Trans‐Blot Turbo Midi 0.2 μm PVDF Transfer Packs (Bio‐Rad) nitrocellulose membrane using the Mix MW function of the Trans‐Blot Turbo Transfer System. .. The membrane was stained with Ponceau S solution (Sigma–Aldrich) and then blocked with 50% Intercept (TBS) Blocking Buffer (Licor) in TBS (Thermo Fisher Scientific). .. Following blocking, the membrane was incubated overnight at 4°C with primary antibodies in v/v 50%/50% Intercept (TBS) Blocking Buffer (Licor)/TBS (Thermo Fisher Scientific) supplemented with 0.1% Tween‐20 (Sigma–Aldrich).

    Article Title: Placental trophoblasts exhibit iron dysregulation and ferroptosis signaling associated with SARS-CoV-2 restriction.
    Article Snippet: Total protein signals were acquired using stain-free technology with a Bio-Rad Chemidoc system. .. Membranes were blocked with TBS Intercept Blocking Buffer (LICORbio, 927–60001) for one hour at room temperature before incubation with primary antibodies (listed in Table S1) overnight at 4◦C. .. Membranes were subsequently washed five times with Tris-buffered saline containing 0.1% Tween 20 (TBST) before incubation with infrared dye-linked fluorescent secondary antibodies (LICORbio, Table S1) for one hour at room temperature.

    Article Title: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness.
    Article Snippet: Immunoblot to detect Cre recombinase expression Cells were lysed in Laemmli buffer, supplemented with protease and phosphatase inhibitors (cOmplete and EDTA-free Protease Inhibitor cocktail; Roche, COEDTAF-RO and PHOSS-RO). .. Proteins were separated in NuPage Bis-Tris gels (Invitrogen, NP0322BOX), transferred on a nitrocellulose membrane (0.2μm; Bio-Rad), and blocked by incubation with Intercept (TBS) Blocking buffer (LI-COR: 927-60001). .. The following primary antibodies were used: anti-Cre recombinase (1:1,000; Cell Signaling, 15036) and anti-α-tubulin (1:5,000; Cell Signaling, 2144).

    Blocking Assay:

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells
    Article Snippet: The proteins from the gel were transferred to a Trans‐Blot Turbo Midi 0.2 μm PVDF Transfer Packs (Bio‐Rad) nitrocellulose membrane using the Mix MW function of the Trans‐Blot Turbo Transfer System. .. The membrane was stained with Ponceau S solution (Sigma–Aldrich) and then blocked with 50% Intercept (TBS) Blocking Buffer (Licor) in TBS (Thermo Fisher Scientific). .. Following blocking, the membrane was incubated overnight at 4°C with primary antibodies in v/v 50%/50% Intercept (TBS) Blocking Buffer (Licor)/TBS (Thermo Fisher Scientific) supplemented with 0.1% Tween‐20 (Sigma–Aldrich).

    Article Title: Acute partner loss enhances social motivation and nucleus accumbens dopamine release in prairie voles
    Article Snippet: .. Membranes were blocked for 1 hour at room temperature (or overnight at 4 degrees) in Intercept Blocking Buffer (LICORbio, 92760001). ..

    Article Title: Placental trophoblasts exhibit iron dysregulation and ferroptosis signaling associated with SARS-CoV-2 restriction.
    Article Snippet: Total protein signals were acquired using stain-free technology with a Bio-Rad Chemidoc system. .. Membranes were blocked with TBS Intercept Blocking Buffer (LICORbio, 927–60001) for one hour at room temperature before incubation with primary antibodies (listed in Table S1) overnight at 4◦C. .. Membranes were subsequently washed five times with Tris-buffered saline containing 0.1% Tween 20 (TBST) before incubation with infrared dye-linked fluorescent secondary antibodies (LICORbio, Table S1) for one hour at room temperature.

    Article Title: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness.
    Article Snippet: Immunoblot to detect Cre recombinase expression Cells were lysed in Laemmli buffer, supplemented with protease and phosphatase inhibitors (cOmplete and EDTA-free Protease Inhibitor cocktail; Roche, COEDTAF-RO and PHOSS-RO). .. Proteins were separated in NuPage Bis-Tris gels (Invitrogen, NP0322BOX), transferred on a nitrocellulose membrane (0.2μm; Bio-Rad), and blocked by incubation with Intercept (TBS) Blocking buffer (LI-COR: 927-60001). .. The following primary antibodies were used: anti-Cre recombinase (1:1,000; Cell Signaling, 15036) and anti-α-tubulin (1:5,000; Cell Signaling, 2144).

    Article Title: Transcriptomic Plasticity and Its Spatial Architecture Shape Melanoma Response to Immunotherapy and Its Combination With BRAF/MEKi
    Article Snippet: PURPOSE In BRAF-mutant melanoma, clinical trials evaluating combination and sequencing strategies of MAPK-targeted therapy with immune checkpoint blockade (ICB) have yielded mixed results.. We sought to define tumor biology associated with resistance to ICB and response to ICB and its combination with MAPK-targeted therapy, and determine biomarkers to guide treatment strategies.. PATIENTS AND METHODS We integrated clinical with multi-omics data from IMspire150, BRIM, AVASTM, and publicly available immunotherapy-treated melanoma cohorts to identify transcriptional states associated with response or resistance to ICB and BRAF/MEK inhibition combined with ICB.

    Article Title: Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag
    Article Snippet: Cell lysateswere run on SDS-PAGE and then transferred to the nitrocellulosemembrane (BioRad #162-0115). .. After the transfer was complete, membranes were allowed to dry overnight, then rehydrated in TBST (10mM Tris, 100mM NaCl, 0.1% Tween 20) and blocked using LI-COR blocking buffer (#927-60001) for 1 h. Primary antibodies targeting SFPQ (Cell Signaling, #ab177149), and control β-actin Mouse mAb #3700 (Cell Signaling, #8H10D10) were diluted 1:2000 in LI-COR buffer supplementedwith0.2%Tween-20 and incubatedwithmembranes overnight with gentle rocking at 4 °C. .. Membranes were then washed 3 times in TBST and incubated with IRDye 800CW Goat anti-Rabbit Ig, (LI-COR, #827-08365) and IRDye 680RD Goat anti-Mouse IgG (LI-COR, #926- 68070) secondary antibodies were diluted 1:20000 in LI-COR buffer supplemented with 0.2% Tween-20 for 45min with gentle rocking at room temperature.

    other:

    Article Title: AI platform for CRISPR functional mapping and function-based drug design
    Article Snippet: Protein was transferred using Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs (BioRad, 1704156).

    Article Title: PACS1 syndrome variant alters the proteomic landscape of developing cortical organoids.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Ctip2 monoclonal antibody (25B6) (recommended dilution 1:500) Abcam Cat #: ab18465; RRID: AB_2064130 Anti-SOX2 polyclonal antibody (recommended dilution 1:200) MilliporeSigma Cat #: AB5603; RRID: AB_2286686 Anti-Nestin Antibody, clone 10C2 (recommended dilution 1:1000) MilliporeSigma Cat #: MAB5326; RRID:AB_2251134 Anti-FOXG1 antibody [EPR18987] (recommended dilution 1:100) Abcam Cat #: ab196868; RRID: AB_2892604 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LICORbio Cat #: 926–32211; RRID AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LICORbio Cat #: 926–68070; RRID AB_10956588 IRDye 800CW Goat anti-Mouse IgG Secondary Antibody LICORbio Cat #: 926–32210; RRID AB_621842 Donkey anti-mouse IgG(H + L) highly crossadsorbed secondary antibody, Alexa Fluor 594 (recommended dilution 1:200) Thermo Fisher Scientific Cat#:A-21203; RRID:AB_141633 Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat #: A32766TR; RRID: AB_2866493 Chemicals, peptides, and recombinant proteins Matrigel hESC-qualified matrix, LDEV-free Corning Cat #: 354277 DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) Gibco Cat #: 11320033 Y-276322HCl Selleck Chemicals Cat #: S1049 Accutase MilliporeSigma Cat #: SCR005 Paraformaldehyde MilliporeSigma Cat #: 158127 Normal donkey serum SouthernBiotech Cat #: 0030–01 Bovine serum albumin MilliporeSigma Cat#: A2153 DAPI Abcam Cat#: ab285390 Fluoromount-GTM Mounting Medium Thermo Fisher Scientific Cat#: 00–4958–02 PVDF Transfer membrane Thermo Fisher Scientific Cat#: 88518 Intercept blocking buffer LICORbio Cat#: 927–60001 Halt Protease & Phosphatase Inhibitor Cocktail (100×) Thermo Fisher Scientific Cat#: 1861284 RIPA Lysis Buffer (10×) EMD Millipore Corp Cat#: 20–188 4× Laemmli Sample Buffer Bio-Rad Cat#: 1610747 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat#: 23225 Critical commercial assays STEMdiffTM Dorsal Forebrain Organoid Differentiation Kit STEM CELL Technologies Cat#:08620 STEMdiffTM Trilineage Differentiation Kit STEM CELL Technologies Cat#: 05230 STEMdiffTM Neural Organoid Maintenance Kit STEM CELL Technologies Cat#: 100–0120 mTeSRTM medium STEM CELL Technologies Cat#: 05825 AggreWellTM800 24-well Plate STEM CELL Technologies Cat#: 34811 (Continued on next page) 14 iScience 29, 117566, October 16, 2026

    Incubation:

    Article Title: Placental trophoblasts exhibit iron dysregulation and ferroptosis signaling associated with SARS-CoV-2 restriction.
    Article Snippet: Total protein signals were acquired using stain-free technology with a Bio-Rad Chemidoc system. .. Membranes were blocked with TBS Intercept Blocking Buffer (LICORbio, 927–60001) for one hour at room temperature before incubation with primary antibodies (listed in Table S1) overnight at 4◦C. .. Membranes were subsequently washed five times with Tris-buffered saline containing 0.1% Tween 20 (TBST) before incubation with infrared dye-linked fluorescent secondary antibodies (LICORbio, Table S1) for one hour at room temperature.

    Article Title: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness.
    Article Snippet: Immunoblot to detect Cre recombinase expression Cells were lysed in Laemmli buffer, supplemented with protease and phosphatase inhibitors (cOmplete and EDTA-free Protease Inhibitor cocktail; Roche, COEDTAF-RO and PHOSS-RO). .. Proteins were separated in NuPage Bis-Tris gels (Invitrogen, NP0322BOX), transferred on a nitrocellulose membrane (0.2μm; Bio-Rad), and blocked by incubation with Intercept (TBS) Blocking buffer (LI-COR: 927-60001). .. The following primary antibodies were used: anti-Cre recombinase (1:1,000; Cell Signaling, 15036) and anti-α-tubulin (1:5,000; Cell Signaling, 2144).

    Article Title: Transcriptomic Plasticity and Its Spatial Architecture Shape Melanoma Response to Immunotherapy and Its Combination With BRAF/MEKi
    Article Snippet: PURPOSE In BRAF-mutant melanoma, clinical trials evaluating combination and sequencing strategies of MAPK-targeted therapy with immune checkpoint blockade (ICB) have yielded mixed results.. We sought to define tumor biology associated with resistance to ICB and response to ICB and its combination with MAPK-targeted therapy, and determine biomarkers to guide treatment strategies.. PATIENTS AND METHODS We integrated clinical with multi-omics data from IMspire150, BRIM, AVASTM, and publicly available immunotherapy-treated melanoma cohorts to identify transcriptional states associated with response or resistance to ICB and BRAF/MEK inhibition combined with ICB.

    Cell-Signaling:

    Article Title: Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag
    Article Snippet: Cell lysateswere run on SDS-PAGE and then transferred to the nitrocellulosemembrane (BioRad #162-0115). .. After the transfer was complete, membranes were allowed to dry overnight, then rehydrated in TBST (10mM Tris, 100mM NaCl, 0.1% Tween 20) and blocked using LI-COR blocking buffer (#927-60001) for 1 h. Primary antibodies targeting SFPQ (Cell Signaling, #ab177149), and control β-actin Mouse mAb #3700 (Cell Signaling, #8H10D10) were diluted 1:2000 in LI-COR buffer supplementedwith0.2%Tween-20 and incubatedwithmembranes overnight with gentle rocking at 4 °C. .. Membranes were then washed 3 times in TBST and incubated with IRDye 800CW Goat anti-Rabbit Ig, (LI-COR, #827-08365) and IRDye 680RD Goat anti-Mouse IgG (LI-COR, #926- 68070) secondary antibodies were diluted 1:20000 in LI-COR buffer supplemented with 0.2% Tween-20 for 45min with gentle rocking at room temperature.

    Control:

    Article Title: Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag
    Article Snippet: Cell lysateswere run on SDS-PAGE and then transferred to the nitrocellulosemembrane (BioRad #162-0115). .. After the transfer was complete, membranes were allowed to dry overnight, then rehydrated in TBST (10mM Tris, 100mM NaCl, 0.1% Tween 20) and blocked using LI-COR blocking buffer (#927-60001) for 1 h. Primary antibodies targeting SFPQ (Cell Signaling, #ab177149), and control β-actin Mouse mAb #3700 (Cell Signaling, #8H10D10) were diluted 1:2000 in LI-COR buffer supplementedwith0.2%Tween-20 and incubatedwithmembranes overnight with gentle rocking at 4 °C. .. Membranes were then washed 3 times in TBST and incubated with IRDye 800CW Goat anti-Rabbit Ig, (LI-COR, #827-08365) and IRDye 680RD Goat anti-Mouse IgG (LI-COR, #926- 68070) secondary antibodies were diluted 1:20000 in LI-COR buffer supplemented with 0.2% Tween-20 for 45min with gentle rocking at room temperature.

    Gentle:

    Article Title: Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag
    Article Snippet: Cell lysateswere run on SDS-PAGE and then transferred to the nitrocellulosemembrane (BioRad #162-0115). .. After the transfer was complete, membranes were allowed to dry overnight, then rehydrated in TBST (10mM Tris, 100mM NaCl, 0.1% Tween 20) and blocked using LI-COR blocking buffer (#927-60001) for 1 h. Primary antibodies targeting SFPQ (Cell Signaling, #ab177149), and control β-actin Mouse mAb #3700 (Cell Signaling, #8H10D10) were diluted 1:2000 in LI-COR buffer supplementedwith0.2%Tween-20 and incubatedwithmembranes overnight with gentle rocking at 4 °C. .. Membranes were then washed 3 times in TBST and incubated with IRDye 800CW Goat anti-Rabbit Ig, (LI-COR, #827-08365) and IRDye 680RD Goat anti-Mouse IgG (LI-COR, #926- 68070) secondary antibodies were diluted 1:20000 in LI-COR buffer supplemented with 0.2% Tween-20 for 45min with gentle rocking at room temperature.



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    Image Search Results


    Details of Reagents Used for TopoI-pS10 IHC.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Developing a Phosphospecific IHC Assay as a Predictive Biomarker for Topoisomerase I Inhibitors

    doi: 10.1369/0022155418766503

    Figure Lengend Snippet: Details of Reagents Used for TopoI-pS10 IHC.

    Article Snippet: Antibody Diluent , Pierce (Thermo Fisher) , 37571 , Pierce Protein-free T20 (TBS) blocking buffer-proprietary formulation in TBS with surfactant and stabilizing agent.

    Techniques: Purification, Modification, Blocking Assay, Formulation