cdnas (New England Biolabs)

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New England Biolabs cdnas
Cdnas, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/program+stata+version+14+statistical+software+program/Terminal+Transferase/pm31881655-250-1-16
Average 98 stars, based on 1301 article reviews

cdnas - by Bioz Stars, 2026-10

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Plasmid Preparation:

Article Title: Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters
Article Snippet: .. The targeting vector was linearized using restriction enzymes, and then the 3′ end of the targeting vector was biotinylated with biotin-16-ddUTP (Roche, Basel, Switzerland) using terminal transferase (NEB Japan, Tokyo, Japan). .. The resulting DNA fragment was purified from the agarose gel, and its biotinylation efficiency was assessed by a depletion experiment using streptavidin magnetic beads (NEB Japan) and found to be close to 100%.

Incubation:

Article Title: Host exonuclease SbcB and a phage-encoded SSB-like protein control activation of the DRT10 reverse transcriptase defense system
Article Snippet: .. The mixture was incubated with terminal transferase (New England Biolabs) and 4 μM dATP to dA-tail 3’ ends. .. An anchored primer (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTTTTTTTTTTV-3’) was annealed to the poly-A tails and extended with exo- Klenow polymerase (New England Biolabs).

Article Title: Direct-to-library methods, systems, and compositions
Article Snippet: The processing without the beads (DTL w/o beads) was performed as follows: Plasma was incubated with 1.6 units of Proteinase K (Sigma-Aldrich, St. Louis, MO) in 1× Terminal Transferase Reaction buffer (NEB, Ipswich, MA) at 60° C. for 20 minutes and 95° C. for 10 minutes and placed on ice until cool. .. One third of the solution was added to the A tailing reaction containing 0.16 mM dATP (NEB, Ipswich, MA) and 20 units Terminal Transferase (NEB, Ipswich, MA) and incubated at 37° C. for 25 minutes and heat inactivated at 75° C. for 10 minutes. ..

Article Title: Direct-to-library methods, systems, and compositions
Article Snippet: .. 40.0 μL of 10× Terminal Transferase Reaction Buffer (NEB, Ipswich, MA), 2.0 μL of 10 mM dATP and 2.0 μL Terminal Transferase (20 u/μL, NEB, Ipswich, MA) was added to prepare the A-tailing reaction which was incubated at 37° C. for 40 min. ..

Article Title: Direct-to-library methods, systems, and compositions
Article Snippet: .. The reaction conditions used were as follows: (i) RNA A-tailing reaction: 50.0 μL of each spiked sample was mixed with a mastermix comprising of 20.0 μL of 10× E. coli Poly(A) polymerase Reaction Buffer (NEB, Ipswich, MA), 6.0 μL of 10 mM ATP and 3.0 μL Poly(A) polymerase (5000 u/μL, NEB, Ipswich, MA) and 121.0 μL Nuclease-free water to prepare the A-tailing reaction which was incubated at 37° C. for 40 min. (ii) DNA A-tailing reaction: 50.0 μL of each spiked sample was mixed with a master mix comprising of 40.0 μL of 10× Terminal Transferase Reaction Buffer (NEB, Ipswich, MA), 2.0 μL of 10% Tween-20 (Thermo-Fisher Scientific, Waltham, MA), 2.0 μL of 10 mM dATP and 2.0 μL Terminal Transferase (20 u/μL, NEB, Ipswich, MA), and 104.0 μL Nuclease-free water to prepare the A-tailing reaction which was incubated at 37° C. for 40 min. (iii) Combined nucleic acid A-tailing reaction: 50.0 μL of each spiked sample was mixed with a master mix comprising of 40.0 μL of 10× Terminal Transferase Reaction Buffer (NEB, Ipswich, MA), 2.0 μL of 10% Tween-20 (Thermo-Fisher Scientific, Waltham, MA), 2.0 μL of 10 mM dATP, 15.0 μL of 10 mM ATP, 2.0 μL Terminal Transferase (20 u/μL, NEB, Ipswich, MA), 1.0 μL Poly(A) polymerase (5000 u/μL, NEB, Ipswich, MA) and 87.0 μL Nuclease-free water to prepare the A-tailing reaction which was incubated at 37° C. for 40 min. ..

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