maxent sequence program (Micromass UK Limited)
Structured Review
Maxent Sequence Program, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/program+maxent/maxent+sequence+program/pm11237698-83-13-14
Average 90 stars, based on 1 article reviews
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Sequencing:Article Title: Copurification of the Lac repressor with polyhistidine-tagged proteins in immobilized metal affinity chromatography. Article Snippet: One of the commonly used resins for immobilized metal affinity purification of polyhistidine-tagged recombinant proteins is TALON resin, a cobalt (II)– carboxymethylaspartate-based matrix linked to Sepharose CL-6B.. Here, we show that TALON resin efficiently purifies the native form of Lac repressor, which represents the major contaminant when (His)6tagged proteins are isolated from Escherichia coli host cells carrying the lacIq gene.. Inspection of the crystal structure of the repressor suggests that three His residues (residues 163, 173, and 202) in each subunit of the tetramer are optimally spaced on an exposed face of the protein to allow interaction with Co(II). |
