luciferase vectors prl-null (Promega)
90
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Promega
luciferase vectors prl-null
Luciferase Vectors Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-+null+vector/pgl3+basic/pmc03594296-172-1-26
Average 90 stars, based on 1 article reviews
Luciferase Vectors Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-+null+vector/pgl3+basic/pmc03594296-172-1-26
Average 90 stars, based on 1 article reviews
luciferase vectors prl-null - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells"
Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells
Journal: PLoS ONE
doi: 10.1371/journal.pone.0058500
Figure Legend Snippet: a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).
Techniques Used: Construct, Plasmid Preparation, Luciferase
Figure Legend Snippet: a) Localization of the different Cx43 3′UTR constructs used in this work. All constructs contain the SV40 promoter (SV40-P) and the Luciferase coding region in addition to the pGL3 polyadenylation signal after the 3′UTR segments (not shown in graphics). The position of a putative polyadenylation signal is indicated. The S1516 region is shown in light grey. b, c) Luciferase assay of the first set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. d, e) Luciferase assay of the second set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05, reported to the pGL3-pr construct).
Techniques Used: Construct, Luciferase
Figure Legend Snippet: Luciferase assay in MCF7 cells transfected with different constructs including the pGL3 control, the pGL3-Pr, in addition to the full length 3′untranslated region (pGL3-Pr-3′UTR) or the S1516 regulatory element (pGL3-Pr-S1516). The experiments were performed at least three times in quadruplicates (* p <0.05).
Techniques Used: Luciferase, Transfection, Construct
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