primescript rt reagent kit (Promega)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Promega primescript rt reagent kit
Primescript Rt Reagent Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+reverse+transcriptase+kit/primescript+rt+reagent+kit/pm38238316-195-21-25
Average 90 stars, based on 1 article reviews

primescript rt reagent kit - by Bioz Stars, 2026-09

90/100 stars

Images

Synthesized:

Article Title: Bisphosphonate-mineralized nano-IFNγ suppresses residual tumor growth caused by incomplete radiofrequency ablation through metabolically remodeling tumor-associated macrophages
Article Snippet: The live/dead dye as well as antibodies involved in the FACS experiment include Zombie Aqua (Biolegend, Catalog No.423101), APC-Cy7 anti-CD45 (Biolegend, Catalog No. 103116), FITC anti-CD11b (Biolegend, Catalog No.101206) and PE anti-F4/80 (Biolegend, Catalog No. 123110). .. The total RNA was extracted using TRIzol® Reagent (Invitrogen, USA) and cDNA was synthesized by a PrimeScript RT Reagent Kit (Promega, USA). ..

Reverse Transcription:

Article Title: Method for determining decrease in functions of hippocampus by using correlation between micro RNA and NMDA receptor, method for inhibiting decrease in functions, and method for screening for inhibitors of decrease in functions
Article Snippet: .. First, PrimeScriptTM RT reagent Kit (Promega ImPromm-II Reverse Transcription System) was used to synthesize cDNA by reverse transcription of RNA of the rat hippocampal tissues of each stage separated in Example 1. ..

Article Title: In Vitro Cytotoxic Mechanism and Exposure Risk Assessments of Banned Organochlorine Pesticides in Indo-Pacific Humpback Dolphins.
Article Snippet: The persistent detrimental effects of banned organochlorine pesticide (dichlorodiphenyltrichloroethanes (DDTs)) exposure are a concern for cetaceans, yet their toxicological effects and risks have been difficult to handle due to technical and ethical issues.. Here, we measured the concentrations of DDTs in the blubber of Indo-Pacific humpback dolphin (Sousa chinensis) (n = 128) collected from the Pearl River Estuary (PRE) during 2003−2020 and studied by an in vitro assay that used the S. chinensis skin fibroblasts to answer questions about the cytotoxicity mechanism and risks of DDTs.. DDT levels in humpback dolphins exceed those reported in other marine mammal studies.

Article Title: Spinal cord injury-derived exosomes exacerbate damage: miR-155-5p mediates inflammatory responses
Article Snippet: Isolation of RNA and quantitative reverse transcription-polymerase chain reaction Total RNA was isolated from spinal cord, BV2 microglia, and exosomes using TRIzol reagent (Invitrogen). .. Subsequently, cDNA synthesis was performed through reverse transcription using the PrimeScriptTM RT Reagent Kit (Promega, Madison, WI, USA) following the manufacturer’s instructions. .. Additionally, miRNAs were reverse-transcribed into cDNA using the miRNA First Strand cDNA Synthesis Kit (Sangon Biotech).

Article Title: Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits.
Article Snippet: The RNA concentration and purity were assessed using Nanodrop 2000 (Thermo Fisher Scientific Corporation, Wilmington, NC, USA). .. Subsequently, total RNA was reverse transcribed to cDNA with the PrimeScript RT kit (Promega Biotechnology Co., Ltd., Beijing, China). .. Finally, quantitative real-time PCR (qRT-PCR) was used to determine the relative gene expression levels under a fluorescence quantitative PCR instrument (Bio-Rad Laboratories Co., Ltd, Shanghai, China), using PerfectStart® Green qPCR SuperMix kit (Promag Beijing Biotechnology Co., Ltd., Beijing, China). qRT-PCR was carried out on the Go Taq® qPCR Master Mix, and the amplification procedure was as follows: initial denaturation at 95 ◦C for 5 min, 40 cycles of denaturation at 95 ◦C for 10 s, annealing at 55–56 ◦C for 10 s, and extension at 72 ◦C for 10 s. The primers were designed and synthesized by Fuzhou Shangya Biotechnology Co., Ltd., Fuzhou, China.

Article Title: 3D-printed advanced scaffold armed with exosomes derived from human skeletal stem cell identified by single-cell RNA sequencing enhances osteochondral regeneration
Article Snippet: Total RNA was extracted from Exos and co-cultured BMSCs (7 days after exosome treatment) using TRIzol reagent (Invitrogen, USA). .. Subsequently, cDNA was reverse transcribed using the PrimeScriptTM RT Reagent Kit (Promega, USA) following the manufacturer's instructions. .. For miRNA, reverse transcription was performed using the miRNA First Strand cDNA Synthesis Kit (Tail Reaction, Sangon Biotech, China).

other:

Article Title: Isolation and pathogenicity of a highly virulent group III porcine Getah virus in China
Article Snippet: RT and PCR were performed using the PrimeScriptTM RT-PCR Kit (Promega, Madison, WI, USA) and PrimeSTAR ® GXL DNA Polymerase, respectively.

cDNA Synthesis:

Concentration Assay:

Article Title: Enhancing m 6 A modification in the motor cortex facilitates corticospinal tract remodeling after spinal cord injury
Article Snippet: Total RNA was extracted from the sensorimotor cortex or neurons using a TRIzol reagent (Invitrogen). .. After the RNA concentration was measured using a microplate reader (Thermofisher, Waltham, MA, USA), RNA was reversed and transcribed into complementary DNA using a PrimeScript TM RT Reagent kit (Promega, Madison, MI, USA). .. Subsequently, a GoTaq qPCR Master Mix kit (Promega) was applied to detect the cycle threshold (Ct).

Review