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primescript ii 1st strand cdna synthesis kit  (Promega)

 
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    Structured Review

    Promega primescript ii 1st strand cdna synthesis kit
    Primescript Ii 1st Strand Cdna Synthesis Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+ii+reverse+transcriptase+kit/primescript+ii+1st+strand+cdna+synthesis+kit/pmc10815410-38-12-19
    Average 90 stars, based on 1 article reviews
    primescript ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: A PR-4 gene identified from Malus domestica is involved in the defense responses against Botryosphaeria dothidea.
    Article Snippet: Pathogenesis-related protein-4 (PR-4) family is a group of proteins with a Barwin domain in C-terminus and generally thought to be involved in plant defense responses.. However, their detailed roles are poorly understood in defense of apple plant against pathogenic infection.. In the present study, a new PR-4 gene (designated as MdPR-4) was identified from Malus domestica, and its roles in defense responses of apple were investigated.

    Article Title: Molecular Characteristics of Bean Common Mosaic Virus Occurring in Inner Mongolia, China
    Article Snippet: .. Briefly, using the RNA samples as templates, cDNAs were synthesized using a PrimeScript II 1st strand cDNA synthesis kit (Promega Biotechnology, Beijing, China) following the manufacturer’s instructions. ..

    cDNA Synthesis:

    Article Title: A PR-4 gene identified from Malus domestica is involved in the defense responses against Botryosphaeria dothidea.
    Article Snippet: Pathogenesis-related protein-4 (PR-4) family is a group of proteins with a Barwin domain in C-terminus and generally thought to be involved in plant defense responses.. However, their detailed roles are poorly understood in defense of apple plant against pathogenic infection.. In the present study, a new PR-4 gene (designated as MdPR-4) was identified from Malus domestica, and its roles in defense responses of apple were investigated.

    Article Title: Extract of Triticum aestivum Sprouts Suppresses Acetaminophen-Induced Hepatotoxicity in Mice by Inhibiting Oxidative Stress
    Article Snippet: .. Total RNA was reverse transcribed into cDNA using PrimeScriptTM II 1st Strand cDNA synthesis kit (Promega, Madison, WI, USA). .. The qRT-PCR was performed using a SYBR Green Master Mix (Thermo Fisher Scientific Inc., Waltham, MA, USA) on StepOne Real-Time PCR System (Applied Biosystems).

    Article Title: Green coffee bean extract improves obesity by decreasing body fat in high-fat diet-induced obese mice.
    Article Snippet: Total RNA of adipose and liver tissues was isolated using the RNeasy mini kit (Qiagen, Velno, Netherlands) according to the manufacturer's protocol. .. The extracted RNA was reverse transcribed to cDNA by using Primescript first strand cDNA synthesis kit (Promega, Fitchburg, USA). .. Then the RNA expression levels of adipogenesis, beta-oxidation, and lipolysis related genes were analyzed by a real-time PCR LightCycler 96 system (Roche, Basel, Switzerland) using a SYBR Green Master PCR Kit (Takara, Shiga, Japan) according to the manufacturer's protocols.

    Article Title: Molecular Characteristics of Bean Common Mosaic Virus Occurring in Inner Mongolia, China
    Article Snippet: .. Briefly, using the RNA samples as templates, cDNAs were synthesized using a PrimeScript II 1st strand cDNA synthesis kit (Promega Biotechnology, Beijing, China) following the manufacturer’s instructions. ..

    Article Title: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion.
    Article Snippet: RNA-level validation of luciferase screens Transfection was performed as described for the luciferase reporter screens, using standard 96-well tissue culture plates (Costar, 3596). .. RNA was isolated from cells using the Direct-zol RNA Miniprep Kit (Zymo Research, R2052). cDNA was generated using the ProtoScript II First Strand cDNA Synthesis Kit (Promega, E6560L). cDNA was amplified using GoTaq Green Master Mix (Promega, M7122), and primers were designed for an amplicon stretching from MAPT exon 9 to the Renilla luciferase ORF. .. Amplicons were run through a 3% SeaKem Agarose Gel (Lonza, 5004) at 100 V for 25 min. Statistical analysis.

    Article Title: Ericoid mycorrhizal fungus enhances microcutting rooting of Rhododendron fortunei and subsequent growth
    Article Snippet: .. The PrimeScript 1st cDNA Synthesis Kit (Promega, WI, USA) was used to synthesize the first strand cDNA. .. RT-qPCR was performed in a 20 μL reaction mixture containing 2× SYBR (PerfeCTa SYBR Green FastMix, Low ROX) 10.0 μL (Quantabio, MD, USA), 1 μL of each corresponding primer for EF1α and the gene of interest, and 1.5 μL of cDNA template (1:5 dilution).

    Article Title: Identification of a xyloglucan-specific endo-(1-4)-beta-D-glucanase inhibitor protein from apple (Malus × domestica Borkh.) as a potential defense gene against Botryosphaeria dothidea.
    Article Snippet: Botryosphaeria dothidea is the causal agent of apple ring rot which is a highly destructive apple disease in China.. Here, a putative xyloglucan-specific endo-(1-4)-beta-d-glucanase inhibitor protein from Malus × domestica (designated as MdXEGIP1) was found to be involved in defense against B. dothidea infection.. MdXEGIP1 shares high amino acid sequence identity with other apple XEGIPs, but exhibited significantly different responses to B. dothidea infection.

    Article Title: Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
    Article Snippet: .. Quantitative RT‐PCR analysis The reverse transcription reactions were performed with the HiScript II 1st Strand cDNA Synthesis Kit (Promega, Madison, Wisconsin, United States). .. Real‐time qPCR analysis was conducted using ChamQ Universal SYBR® qPCR Master Mix (Vazyme, Nanjing, China) on a CFX Connect Real‐Time System (Bio‐Rad, Hercules, California, United States).

    Reverse Transcription:

    Article Title: Extract of Triticum aestivum Sprouts Suppresses Acetaminophen-Induced Hepatotoxicity in Mice by Inhibiting Oxidative Stress
    Article Snippet: .. Total RNA was reverse transcribed into cDNA using PrimeScriptTM II 1st Strand cDNA synthesis kit (Promega, Madison, WI, USA). .. The qRT-PCR was performed using a SYBR Green Master Mix (Thermo Fisher Scientific Inc., Waltham, MA, USA) on StepOne Real-Time PCR System (Applied Biosystems).

    Article Title: Green coffee bean extract improves obesity by decreasing body fat in high-fat diet-induced obese mice.
    Article Snippet: Total RNA of adipose and liver tissues was isolated using the RNeasy mini kit (Qiagen, Velno, Netherlands) according to the manufacturer's protocol. .. The extracted RNA was reverse transcribed to cDNA by using Primescript first strand cDNA synthesis kit (Promega, Fitchburg, USA). .. Then the RNA expression levels of adipogenesis, beta-oxidation, and lipolysis related genes were analyzed by a real-time PCR LightCycler 96 system (Roche, Basel, Switzerland) using a SYBR Green Master PCR Kit (Takara, Shiga, Japan) according to the manufacturer's protocols.

    Article Title: Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
    Article Snippet: .. Quantitative RT‐PCR analysis The reverse transcription reactions were performed with the HiScript II 1st Strand cDNA Synthesis Kit (Promega, Madison, Wisconsin, United States). .. Real‐time qPCR analysis was conducted using ChamQ Universal SYBR® qPCR Master Mix (Vazyme, Nanjing, China) on a CFX Connect Real‐Time System (Bio‐Rad, Hercules, California, United States).

    Isolation:

    Article Title: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion.
    Article Snippet: RNA-level validation of luciferase screens Transfection was performed as described for the luciferase reporter screens, using standard 96-well tissue culture plates (Costar, 3596). .. RNA was isolated from cells using the Direct-zol RNA Miniprep Kit (Zymo Research, R2052). cDNA was generated using the ProtoScript II First Strand cDNA Synthesis Kit (Promega, E6560L). cDNA was amplified using GoTaq Green Master Mix (Promega, M7122), and primers were designed for an amplicon stretching from MAPT exon 9 to the Renilla luciferase ORF. .. Amplicons were run through a 3% SeaKem Agarose Gel (Lonza, 5004) at 100 V for 25 min. Statistical analysis.

    Generated:

    Article Title: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion.
    Article Snippet: RNA-level validation of luciferase screens Transfection was performed as described for the luciferase reporter screens, using standard 96-well tissue culture plates (Costar, 3596). .. RNA was isolated from cells using the Direct-zol RNA Miniprep Kit (Zymo Research, R2052). cDNA was generated using the ProtoScript II First Strand cDNA Synthesis Kit (Promega, E6560L). cDNA was amplified using GoTaq Green Master Mix (Promega, M7122), and primers were designed for an amplicon stretching from MAPT exon 9 to the Renilla luciferase ORF. .. Amplicons were run through a 3% SeaKem Agarose Gel (Lonza, 5004) at 100 V for 25 min. Statistical analysis.

    Amplification:

    Article Title: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion.
    Article Snippet: RNA-level validation of luciferase screens Transfection was performed as described for the luciferase reporter screens, using standard 96-well tissue culture plates (Costar, 3596). .. RNA was isolated from cells using the Direct-zol RNA Miniprep Kit (Zymo Research, R2052). cDNA was generated using the ProtoScript II First Strand cDNA Synthesis Kit (Promega, E6560L). cDNA was amplified using GoTaq Green Master Mix (Promega, M7122), and primers were designed for an amplicon stretching from MAPT exon 9 to the Renilla luciferase ORF. .. Amplicons were run through a 3% SeaKem Agarose Gel (Lonza, 5004) at 100 V for 25 min. Statistical analysis.

    Luciferase:

    Article Title: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion.
    Article Snippet: RNA-level validation of luciferase screens Transfection was performed as described for the luciferase reporter screens, using standard 96-well tissue culture plates (Costar, 3596). .. RNA was isolated from cells using the Direct-zol RNA Miniprep Kit (Zymo Research, R2052). cDNA was generated using the ProtoScript II First Strand cDNA Synthesis Kit (Promega, E6560L). cDNA was amplified using GoTaq Green Master Mix (Promega, M7122), and primers were designed for an amplicon stretching from MAPT exon 9 to the Renilla luciferase ORF. .. Amplicons were run through a 3% SeaKem Agarose Gel (Lonza, 5004) at 100 V for 25 min. Statistical analysis.

    RNA Extraction:

    Article Title: Identification of a xyloglucan-specific endo-(1-4)-beta-D-glucanase inhibitor protein from apple (Malus × domestica Borkh.) as a potential defense gene against Botryosphaeria dothidea.
    Article Snippet: Botryosphaeria dothidea is the causal agent of apple ring rot which is a highly destructive apple disease in China.. Here, a putative xyloglucan-specific endo-(1-4)-beta-d-glucanase inhibitor protein from Malus × domestica (designated as MdXEGIP1) was found to be involved in defense against B. dothidea infection.. MdXEGIP1 shares high amino acid sequence identity with other apple XEGIPs, but exhibited significantly different responses to B. dothidea infection.

    Quantitative RT-PCR:

    Article Title: Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
    Article Snippet: .. Quantitative RT‐PCR analysis The reverse transcription reactions were performed with the HiScript II 1st Strand cDNA Synthesis Kit (Promega, Madison, Wisconsin, United States). .. Real‐time qPCR analysis was conducted using ChamQ Universal SYBR® qPCR Master Mix (Vazyme, Nanjing, China) on a CFX Connect Real‐Time System (Bio‐Rad, Hercules, California, United States).



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