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TransGen biotech co primescript rt reagent kit
Primescript Rt Reagent Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+ii+reverse+transcriptase+kit/cdna+synthesis+kit/pm38177310-238-19-24
Average 90 stars, based on 1 article reviews
primescript rt reagent kit - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Mefunidone alleviates silica-induced inflammation and fibrosis by inhibiting the TLR4-NF-κB/MAPK pathway and attenuating pyroptosis in murine macrophages.
Article Snippet: Aims: Silicosis is the most common and severe type of pneumoconiosis, imposing a substantial disease burden and economic loss on patients and society.. The pathogenesis and key targets of silicosis are not yet clear, and there are currently no effective treatments available.. Therefore, we conducted research on mefunidone (MFD), a novel antifibrotic drug, to explore its efficacy and mechanism of action in murine silicosis.

Article Title: Total Syntheses and Anti-Inflammatory Studies of Three Natural Coumarins: Glycycoumarin, Glycyrin, and 3- O -Methylglycyrol
Article Snippet: Total RNA (2 μg) was reverse-transcribed using TransScript ® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (Cat No: AT341-01, TransGen Biotech, Beijing, China).

RNA Extraction:

Article Title: Deoxynivalenol induces m 6 A-mediated upregulation of p21 and growth arrest of mouse hippocampal neuron cells in vitro
Article Snippet: .. Total RNA extraction from HT-22 cells using Total RNA Extraction Reagent (R401-01, Vazyme Biotech, China), and the cDNA was synthesized using the Reverse Transcription Kit (AU341, TransGen Biotech, China). .. Gene expression was assessed using a QuantStudioTM 6 Flex real-time PCR system (Thermo Scientific, USA).

Synthesized:

Article Title: Deoxynivalenol induces m 6 A-mediated upregulation of p21 and growth arrest of mouse hippocampal neuron cells in vitro
Article Snippet: .. Total RNA extraction from HT-22 cells using Total RNA Extraction Reagent (R401-01, Vazyme Biotech, China), and the cDNA was synthesized using the Reverse Transcription Kit (AU341, TransGen Biotech, China). .. Gene expression was assessed using a QuantStudioTM 6 Flex real-time PCR system (Thermo Scientific, USA).

Reverse Transcription:

Article Title: Deoxynivalenol induces m 6 A-mediated upregulation of p21 and growth arrest of mouse hippocampal neuron cells in vitro
Article Snippet: .. Total RNA extraction from HT-22 cells using Total RNA Extraction Reagent (R401-01, Vazyme Biotech, China), and the cDNA was synthesized using the Reverse Transcription Kit (AU341, TransGen Biotech, China). .. Gene expression was assessed using a QuantStudioTM 6 Flex real-time PCR system (Thermo Scientific, USA).

Article Title: METTL14-mediated upregulation of lncRNA HOTAIR represses PP1α expression by promoting H3K4me1 demethylation in oxycodone-treated mice.
Article Snippet: Images obtained using Nikon Ci confocal microscopy (Japan) with excitation wavelengths 405, 488, and 561 nm were analyzed with ImageJ software (NIH). .. Total RNA in the striatum (n = 4 animals in each group) was extracted using TRIzol reagent (Cat# 12183555; ThermoFisher, USA) and reverse- transcribed into cDNA using an EasyScript Kit (Cat# AE341- 02; TransGen Biotech, China). .. Real- time PCR (Stratagene Mx3000P; Agilent Technologies, Germany) was performed in triplicate on a 20 μL sample (0.4 μL ROX reference dye, 10 μM primers, 10 ng DNA, 10 μL 2× SYBR Green qPCR Master Mix).

Article Title: Injectable citrate-based polyurethane-urea as a tug-of-war-inspired bioactive self-expansive and planar-fixing screw augmented bone-tendon healing
Article Snippet: .. On the 7th day, the cells were harvested, the total cellular RNA was extracted by lysis in Trizol (TransGen Biotech, Beijing), which was then reverse-transcribed into cDNA with a cDNA Reverse Transcription Kit (TransGen, China). .. The qPCR was performed using PerfectStart® Fast Green qPCR SuperMix (TransGen, China) with QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, USA).

Article Title: Mechanism of Mitochondrial Kinetic Imbalance and Nrf2 Signaling Pathway-Mediated Oxidative Stress in Nickel and/or Chromium-Induced Kidney Injury in Mice.
Article Snippet: Total kidney RNA was extracted according to the instructions of the TransZol Up Plus RNA Kit (Takara Bio, Kusatsu, Shiga, Japan, Cat. No: 9109). .. The extracted total RNA was reverse transcribed to synthesize cDNA, following the instructions provided by the reverse transcription kit (TransGen Biotech Co., Ltd., Beijing, China, Cat. No: AU341-02). .. Real-time qPCR reactions were carried out using cDNA as a template and the amplification system according to the instruction manual of the qRT-PCR kit (Vazyme Biotech Co., Ltd., Nanjing, China, Cat. No: Q141-02).

Article Title: METTL14 ‐mediated upregulation of lncRNA HOTAIR represses PP1α expression by promoting H3K4me1 demethylation in oxycodone‐treated mice
Article Snippet: Images obtained using Nikon Ci confocal microscopy (Japan) with excitation wavelengths 405, 488, and 561 nm were analyzed with ImageJ software (NIH). .. Total RNA in the striatum ( n = 4 animals in each group) was extracted using TRIzol reagent (Cat# 12183555; ThermoFisher, USA) and reverse‐transcribed into cDNA using an EasyScript Kit (Cat# AE341‐02; TransGen Biotech, China). .. Real‐time PCR (Stratagene Mx3000P; Agilent Technologies, Germany) was performed in triplicate on a 20 μL sample (0.4 μL ROX reference dye, 10 μM primers, 10 ng DNA, 10 μL 2× SYBR Green qPCR Master Mix).

cDNA Synthesis:

Article Title: Integrated Transcriptomic and Metabolomic Analysis Revealed Abscisic Acid-Induced Regulation of Monoterpene Biosynthesis in Grape Berries.
Article Snippet: .. DNase-treated RNA was transcribed with a cDNA synthesis kit to confirm the accuracy of the transcriptome analysis (TransGen Biotech, Beijing, China). .. The qRT–PCR iQ5 Connect real-time system was operated on a ChamQ SYBR qPCR Master Mix (Vazyme#Q311, Nanjing, China) (Bio-Rad, Hercules, CA, USA).

Lysis:

Article Title: Injectable citrate-based polyurethane-urea as a tug-of-war-inspired bioactive self-expansive and planar-fixing screw augmented bone-tendon healing
Article Snippet: .. On the 7th day, the cells were harvested, the total cellular RNA was extracted by lysis in Trizol (TransGen Biotech, Beijing), which was then reverse-transcribed into cDNA with a cDNA Reverse Transcription Kit (TransGen, China). .. The qPCR was performed using PerfectStart® Fast Green qPCR SuperMix (TransGen, China) with QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, USA).



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