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Beijing CWBio primescript™ rt reagent kit
Primescript™ Rt Reagent Kit, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+ii+reverse+transcriptase+kit/primescript+rt+reagent+kit/pm34737155-58-40-44
Average 90 stars, based on 1 article reviews
primescript™ rt reagent kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

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Article Title: Characterization of metallothionein genes from Broussonetia papyrifera : metal binding and heavy metal tolerance mechanisms
Article Snippet: Reverse transcription of RNA to cDNA was performed using the PrimeScriptTM RT reagent Kit (CWBIO, Beijing, China).

Article Title: Light-regulated microRNAs shape dynamic gene expression in the zebrafish circadian clock.
Article Snippet: Total RNA was extracted from zebrafish larvae using the TRIzol Reagent as previously described [17,19]. mRNA was reverse transcribed into cDNA using the PrimeScript RT PLOS Genetics | https://doi.org/10.1371/journal.pgen.1011545 January 8, 2025 4 / 27 Reagent Kit (CWBIO, China), while miRNA was reverse transcribed using the All-in-One miRNA First-Strand cDNA Synthesis Kit (Genecopoeia, USA).

Real-time Polymerase Chain Reaction:

Article Title: Probiotics fermentation enhanced the bioactive properties of Gnaphalium affine water extract and improved regulation ability of gut microbiota
Article Snippet: E. coli (ATCC 25922) and S. aureus (ATCC 25923) were maintained at Key Laboratory of Pathogenic Biology, Guizhou Medical University. .. The PrimeScript RT Master Kit and SYBR Real-time PCR Kit were purchased from CoWin Biological Technology (CWBIO) Co., Ltd. (Jiangsu, China). .. TRIzol reagent and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Reverse Transcription:

Article Title: Dietary Gnaphalium affine supplementation prevents fat accumulation and inflammation by regulating composition and metabolism of gut microbiota in high fat diet fed mice
Article Snippet: .. Reverse transcription of 1 μg RNA was performed using PrimeScript RT Master Kit (CWBIO, China). .. Gene expression was measured in a the CFX Connect Real-Time PCR detection system (Bio-Rad) with SYBR Real-time PCR Kit (CWBIO, China).

Article Title: JrMYB44 is required for the accumulation of polyphenols and contributes to drought tolerance in Juglans regia
Article Snippet: .. The purified RNA was reverse-transcribed into cDNA using PrimeScript TM RT reagent Kit (CWBIO, Beijing, China). .. The cDNA was diluted to 1/10 and used as the template of RT-qPCR, which was implemented using the SYBR Green Real time PCR Master mix (CWBIO) and carried out on a CFX96 TouchTM Real-Time PCR Detection System (Bio-Rad Laboratories, Redmond, WA).

Article Title: Babaodan inhibits cell proliferation and metastasis and enhances anti-tumor effects of camrelizumab by inhibiting M2 phenotype macrophages in hepatocellular carcinoma.
Article Snippet: Ethnopharmacological relevance: Babaodan (BBD) is a unique Chinese medication utilized in traditional Chinese medicine.. It can eliminate toxins, induce diuresis, and eliminate yellowish hue.. In addition to treating acute and chronic viral hepatitis, cholecystitis, cholangitis, and urinary tract infections, BBD has garnered popularity as a substitution treatment for several malignant cancers, particularly hepatocellular carcinoma (HCC).

Article Title: Light-regulated microRNAs shape dynamic gene expression in the zebrafish circadian clock
Article Snippet: Comparative Sequence Analysis: pre-miRNA sequences from zebrafish ( Danio rerio ), human ( Homo sapiens ), mouse ( Mus musculus ), African clawed frog ( Xenopus leavis ), Carolina anole ( Anolis carolinensis ), Japanese medaka ( Oryzias latipes ) and sea Lamprey ( Petromyzon marinus ) were obtained from the miRBase database and aligned using the MEGA software (version 7.0) [ ]. .. Total RNA was extracted from zebrafish larvae using the TRIzol Reagent as previously described [ , ]. mRNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (CWBIO, China), while miRNA was reverse transcribed using the All-in-One miRNA First-Strand cDNA Synthesis Kit (Genecopoeia, USA). .. Quantitative real-time PCR (qRT-PCR) analyses were performed using the SYBR Green Kit (CWBIO, China) with three biological replicates per group.

Purification:

Article Title: JrMYB44 is required for the accumulation of polyphenols and contributes to drought tolerance in Juglans regia
Article Snippet: .. The purified RNA was reverse-transcribed into cDNA using PrimeScript TM RT reagent Kit (CWBIO, Beijing, China). .. The cDNA was diluted to 1/10 and used as the template of RT-qPCR, which was implemented using the SYBR Green Real time PCR Master mix (CWBIO) and carried out on a CFX96 TouchTM Real-Time PCR Detection System (Bio-Rad Laboratories, Redmond, WA).

cDNA Synthesis:

Article Title: Light-regulated microRNAs shape dynamic gene expression in the zebrafish circadian clock
Article Snippet: Comparative Sequence Analysis: pre-miRNA sequences from zebrafish ( Danio rerio ), human ( Homo sapiens ), mouse ( Mus musculus ), African clawed frog ( Xenopus leavis ), Carolina anole ( Anolis carolinensis ), Japanese medaka ( Oryzias latipes ) and sea Lamprey ( Petromyzon marinus ) were obtained from the miRBase database and aligned using the MEGA software (version 7.0) [ ]. .. Total RNA was extracted from zebrafish larvae using the TRIzol Reagent as previously described [ , ]. mRNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (CWBIO, China), while miRNA was reverse transcribed using the All-in-One miRNA First-Strand cDNA Synthesis Kit (Genecopoeia, USA). .. Quantitative real-time PCR (qRT-PCR) analyses were performed using the SYBR Green Kit (CWBIO, China) with three biological replicates per group.



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