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primescripttm rtreagent kit with gdnaeraser  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm rtreagent kit with gdnaeraser
    Primescripttm Rtreagent Kit With Gdnaeraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rtreagent+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pm41350926-237-7-13
    Average 99 stars, based on 14775 article reviews
    primescripttm rtreagent kit with gdnaeraser - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Resveratrol supplementation mitigates age-related declines in egg production, quality, and ovarian health of laying hens
    Article Snippet: .. Complementary DNA (cDNA) synthesis was performed with the PrimeScript RT Reagent Kit (RR047A, TaKaRa Bio Inc., Dalian, Liaoning, China) with genomic DNA removal performed. .. Quantitative real-time PCR amplification was conducted on an ABI Prism 7000 system (Applied Biosystems, Foster City, CA, USA) using SYBR Premix Ex Taq II (Takara Bio Inc., Dalian, Liaoning, China).

    Article Title: Heterologous production of epothilones in Streptomyces albus J1074 via promoter engineering of the biosynthetic gene cluster and precursor supply enhancement
    Article Snippet: Total RNA was extracted using the RNAprep Pure Kit (Tiangen Biotech (Beijing) Co., Ltd., cat. no. DP430). .. Genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis were performed using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio Inc., Shiga, Japan; cat. no. RR047A). .. Real-time PCR was carried out on a StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA) using TB Green Premix Ex Taq (Takara Bio Inc., cat. no. RR420A), following the manufacturer's protocol.

    Article Title: From feed to fillet: Dietary lipid affects sensory quality and fillet lipid profile by modulating lipid metabolism in triploid rainbow trout ( Oncorhynchus mykiss )
    Article Snippet: For triploid rainbow trout in the MF and MS groups, total RNA was isolated using TRIzol reagent (9109, TaKaRa, Japan). .. The cDNA template was prepared by reverse transcription with the PrimeScriptTM RT regern Kit strand cDNA synthesis kit (RR047A, TaKaRa, Japan). ..

    Reverse Transcription:

    Article Title: From feed to fillet: Dietary lipid affects sensory quality and fillet lipid profile by modulating lipid metabolism in triploid rainbow trout ( Oncorhynchus mykiss )
    Article Snippet: For triploid rainbow trout in the MF and MS groups, total RNA was isolated using TRIzol reagent (9109, TaKaRa, Japan). .. The cDNA template was prepared by reverse transcription with the PrimeScriptTM RT regern Kit strand cDNA synthesis kit (RR047A, TaKaRa, Japan). ..

    Article Title: BUD23 is associated with malignancy and correlates with immune infiltration in NSCLC
    Article Snippet: RNA was extracted from the cells using TRIzol ® (Invitrogen; Thermo Fisher Scientific, Inc.). .. RT was performed using the PrimeScript TM RT Reagent Kit with gDNA Eraser (Perfect Real Time; cat. no. RR047A; TaKaRa Bio Inc.) containing reverse transcriptase, RT buffer, dNTP mix, oligo(dT) primers, random primers and gDNA Eraser. ..

    Article Title: Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein response
    Article Snippet: Total RNA was extracted from cells or gingival tissues using RNAiso Plus (9108/9109, Takara). .. Two micrograms of RNA per sample were reverse transcribed into cDNA using the PrimeScriptTM RT Reagent Kit (RR047A, Takara). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (RK21203, Abclonal) on a CFX96 Real-Time PCR Detection System (BIO-RAD) following the manufacturer's protocol.

    Article Title: 3D-printing flexible PLGA scaffold modified with bioorthogonal IGF-1 for skin regeneration
    Article Snippet: On day 7, total RNA was extracted using TRIzol Reagent (Invitrogen). .. And the reverse transcription was governed using PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time, TaKaRa). ..

    Staining:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    TUNEL Assay:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Apoptosis Assay:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Polymer:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Polymerase Chain Reaction:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Isolation:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Purification:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..



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