primescript rt kit (TaKaRa)
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primescript rt kit
Primescript Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+reagent+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pm42844252-463-15-22
Average 99 stars, based on 15291 article reviews
Primescript Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+reagent+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pm42844252-463-15-22
Average 99 stars, based on 15291 article reviews
primescript rt kit - by Bioz Stars,
2026-10
99/100 stars
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cDNA Synthesis:Article Title: Genome-wide identification of the TIFY gene family in Sorghum bicolor and functional characterization of SbTIFY9 in salt stress tolerance. Article Snippet: The TIFY gene family is a plant-specific group of transcriptional regulators characterized by a conserved TIFY domain (TIF[F/Y]XG), participating in plant development, phytohormone signaling and abiotic stress responses.. In this study, 21 SbTIFY genes were genome-widely identified in sorghum (Sorghum bicolor L.).. According to phylogeny and protein structures, SbTIFY proteins were grouped into JAZ and ZML subfamilies, and the JAZ subfamily was further divided into five clades. Quantitative RT-PCR:Article Title: Cell-type-specific PIN-FORMED 3-mediated polar auxin transport regulates cambial activity and tension wood formation in Populus tomentosa. Article Snippet: Auxin is a central regulator of tree secondary growth and forms maxima in the vascular cambium, yet how these maxima are established remains elusive.. Here, we functionally characterized two redundant PIN-FORMED 3 (PIN3) paralogs from Populus tomentosa to define their roles in secondary growth and gravity-induced tension wood (TW) formation.. We employed fluorescent reporter lines, clustered regularly interspaced short palindromic repeats-generated knockout mutants, phloem-specific RNA interference transgenic plants and isotopic auxin transport assays to dissect PIN3 functions during secondary growth of poplar stems. Isolation:Article Title: Cell-type-specific PIN-FORMED 3-mediated polar auxin transport regulates cambial activity and tension wood formation in Populus tomentosa. Article Snippet: Auxin is a central regulator of tree secondary growth and forms maxima in the vascular cambium, yet how these maxima are established remains elusive.. Here, we functionally characterized two redundant PIN-FORMED 3 (PIN3) paralogs from Populus tomentosa to define their roles in secondary growth and gravity-induced tension wood (TW) formation.. We employed fluorescent reporter lines, clustered regularly interspaced short palindromic repeats-generated knockout mutants, phloem-specific RNA interference transgenic plants and isotopic auxin transport assays to dissect PIN3 functions during secondary growth of poplar stems. RNA Extraction:Article Title: Cell-type-specific PIN-FORMED 3-mediated polar auxin transport regulates cambial activity and tension wood formation in Populus tomentosa. Article Snippet: Auxin is a central regulator of tree secondary growth and forms maxima in the vascular cambium, yet how these maxima are established remains elusive.. Here, we functionally characterized two redundant PIN-FORMED 3 (PIN3) paralogs from Populus tomentosa to define their roles in secondary growth and gravity-induced tension wood (TW) formation.. We employed fluorescent reporter lines, clustered regularly interspaced short palindromic repeats-generated knockout mutants, phloem-specific RNA interference transgenic plants and isotopic auxin transport assays to dissect PIN3 functions during secondary growth of poplar stems. Article Title: A Macrophage-Integrated 3D Spheroid Model Inoculated with Bacteria for Simultaneous Evaluation of Antibacterial Efficacy, Cytotoxicity, and Immunomodulation. Article Snippet: Conventional antimicrobial-material testing typically relies on separate bacterial assays and two-dimensional (2D) mammalian cell cultures.. Although essential as first-line screening tools, these approaches do not capture the three-dimensional (3D) tissue architecture, diffusion constraints, extracellular-matrix remodeling, and innate immune interactions encountered by host-contacting antimicrobial materials.. Herein, this study introduces a macrophage-containing 3D spheroid model designed for the simultaneous evaluation of antimicrobial efficacy, host-cell cytotoxicity, and immune responses. Synthesized:Article Title: Cell-type-specific PIN-FORMED 3-mediated polar auxin transport regulates cambial activity and tension wood formation in Populus tomentosa. Article Snippet: Auxin is a central regulator of tree secondary growth and forms maxima in the vascular cambium, yet how these maxima are established remains elusive.. Here, we functionally characterized two redundant PIN-FORMED 3 (PIN3) paralogs from Populus tomentosa to define their roles in secondary growth and gravity-induced tension wood (TW) formation.. We employed fluorescent reporter lines, clustered regularly interspaced short palindromic repeats-generated knockout mutants, phloem-specific RNA interference transgenic plants and isotopic auxin transport assays to dissect PIN3 functions during secondary growth of poplar stems. Article Title: Deoxythymidylate Kinase (DTYMK)-Driven Pyrimidine Hypermetabolism Is Associated with a Folate-Dependent Macrophage Programme in Triple-Negative Breast Cancer. Article Snippet: RT-qPCR Total RNA was isolated from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, MA, USA, cat# 15596018CN), and RNA concentration and purity were assessed via a NanoDrop spectrophotometer. .. Complementary DNA (cDNA) was synthesized employing the PrimeScript RT reagent Kit with Article Title: Non-coding RNA levels integrating analysis revealed the salt and drought stress response mechanism of miR395e-PvUXS1 pathway in switchgrass. Article Snippet: Soil salinization and drought are major constraints on global agricultural production, yet the shared molecular mechanisms by which forage crops respond to these stresses remain incompletedly understood.. Identifying candidate genes with potential value for stress-resilient breeding is therefore an important research objective.. Switchgrass (Panicum virgatum L.) is a perennial forage and bioenergy crop with adaptation to saline, semi-arid, and drought-prone environments. Reverse Transcription:Article Title: PA4933 Mediates Dual Regulation of Oxidative Resistance and Virulence in Pseudomonas aeruginosa Pulmonary Infection. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Analysis:Article Title: Non-coding RNA levels integrating analysis revealed the salt and drought stress response mechanism of miR395e-PvUXS1 pathway in switchgrass. Article Snippet: Soil salinization and drought are major constraints on global agricultural production, yet the shared molecular mechanisms by which forage crops respond to these stresses remain incompletedly understood.. Identifying candidate genes with potential value for stress-resilient breeding is therefore an important research objective.. Switchgrass (Panicum virgatum L.) is a perennial forage and bioenergy crop with adaptation to saline, semi-arid, and drought-prone environments. |