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EZBioscience primescript rt master mix
Primescript Rt Master Mix, supplied by EZBioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+kit/primescript+rt+reagent+kit/pmc09388789-115-43-57
Average 90 stars, based on 1 article reviews
primescript rt master mix - by Bioz Stars, 2026-11
90/100 stars

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Reverse Transcription:

Article Title: A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder
Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (RT-qPCR): Total RNA of the synovial fibroblasts was isolated using an RNA Purification Kit (EZBioscience, USA) and transcribed into cDNA with a PrimeScript RT reagent kit (EZBioscience, USA). .. RT-qPCR analyses were then performed using a LightCycler 480 RealTime PCR System (Roche, Basel, Switzerland) with a SYBR Premix Ex Taq (Accurate Biology, China).

Article Title: The Oncogenic and Prognostic Significance of NSUN5 in Pan-Cancer and Its Potential roles in Clear Renal Cell Carcinoma
Article Snippet: .. RNA isolation and quantitative real time polymerasechain reaction (qRT‐PCR) We extracted total RNA from cell lines using TRIzol Reagent (Invitrogen, USA), and then utilized PrimeScriptTM RT reagent Kit (EZBioscience, China) for reverse transcription to produce cDNA. qRT-PCR was nished using the SYBR Green PCR reagent (EZBioscience, China). ..

Article Title: Machine learning-derived natural killer cell signature predicts prognosis and therapeutic response in clear cell renal cell carcinoma.
Article Snippet: Background: Natural killer cells, interconnected with patient prognosis and treatment response, play a pivotal role in the tumor immune microenvironment and may serve as potential novel predictive biomarkers for renal

Article Title: Crosstalk of disulfidptosis-related subtypes identifying a prognostic signature to improve prognosis and immunotherapy responses of clear cell renal cell carcinoma patients.
Article Snippet: The TRIzol Reagent (Invitrogen, USA) was applied to extract total cellular RNA according to the protocol. .. RNA was reverse transcribed to cDNA by the PrimeScript RT reagent kit (EZBioscience, China). .. EZBioscience 2 × SYBR Green qPCR Master Mix (EZBioscience, China) conducted the procedure.

Article Title: A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder.
Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (RTqPCR): Total RNA of the synovial fibroblasts was isolated using an RNA Purification Kit (EZBioscience, USA) and transcribed into cDNA with a PrimeScript RT reagent kit (EZBioscience, USA). .. RT-qPCR analyses were then performed using a LightCycler 480 RealTime PCR System (Roche, Basel, Switzerland) with a SYBR Premix Ex Taq (Accurate Biology, China).

Article Title: Proline-driven metabolic reprogramming promotes skeletal muscle hypertrophy and oxidative myofiber specification in porcine offspring: a stage-optimized maternal nutritional intervention.
Article Snippet: .. After reverse transcription using PrimerScript RT reagent Kit (EZBioscience, Guangzhou, China), RT-qPCR was performed to analyze the expression levels of related genes on a QuantStudio 6 RealTime PCR System (Thermo Fisher, Waltham, USA). ..

Article Title: Integrated bulk RNA and single-cell analysis with experimental validation reveal oxidative stress-related diagnostic biomarkers for osteoporosis.
Article Snippet: .. RNA was reverse transcribed to cDNA by the PrimeScript RT reagent kit (BL699A, EZBioscience, China). .. EZBioscience 2 × SYBR Green qPCR Master Mix (A301-10, GenStar, China) conducted the procedure.

Polymerase Chain Reaction:

Article Title: A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder
Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (RT-qPCR): Total RNA of the synovial fibroblasts was isolated using an RNA Purification Kit (EZBioscience, USA) and transcribed into cDNA with a PrimeScript RT reagent kit (EZBioscience, USA). .. RT-qPCR analyses were then performed using a LightCycler 480 RealTime PCR System (Roche, Basel, Switzerland) with a SYBR Premix Ex Taq (Accurate Biology, China).

Article Title: The Oncogenic and Prognostic Significance of NSUN5 in Pan-Cancer and Its Potential roles in Clear Renal Cell Carcinoma
Article Snippet: .. RNA isolation and quantitative real time polymerasechain reaction (qRT‐PCR) We extracted total RNA from cell lines using TRIzol Reagent (Invitrogen, USA), and then utilized PrimeScriptTM RT reagent Kit (EZBioscience, China) for reverse transcription to produce cDNA. qRT-PCR was nished using the SYBR Green PCR reagent (EZBioscience, China). ..

Article Title: Machine learning-derived natural killer cell signature predicts prognosis and therapeutic response in clear cell renal cell carcinoma.
Article Snippet: Background: Natural killer cells, interconnected with patient prognosis and treatment response, play a pivotal role in the tumor immune microenvironment and may serve as potential novel predictive biomarkers for renal

Article Title: A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder.
Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (RTqPCR): Total RNA of the synovial fibroblasts was isolated using an RNA Purification Kit (EZBioscience, USA) and transcribed into cDNA with a PrimeScript RT reagent kit (EZBioscience, USA). .. RT-qPCR analyses were then performed using a LightCycler 480 RealTime PCR System (Roche, Basel, Switzerland) with a SYBR Premix Ex Taq (Accurate Biology, China).

Article Title: Proline-driven metabolic reprogramming promotes skeletal muscle hypertrophy and oxidative myofiber specification in porcine offspring: a stage-optimized maternal nutritional intervention.
Article Snippet: .. After reverse transcription using PrimerScript RT reagent Kit (EZBioscience, Guangzhou, China), RT-qPCR was performed to analyze the expression levels of related genes on a QuantStudio 6 RealTime PCR System (Thermo Fisher, Waltham, USA). ..

Isolation:

Article Title: A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder
Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (RT-qPCR): Total RNA of the synovial fibroblasts was isolated using an RNA Purification Kit (EZBioscience, USA) and transcribed into cDNA with a PrimeScript RT reagent kit (EZBioscience, USA). .. RT-qPCR analyses were then performed using a LightCycler 480 RealTime PCR System (Roche, Basel, Switzerland) with a SYBR Premix Ex Taq (Accurate Biology, China).

Article Title: The Oncogenic and Prognostic Significance of NSUN5 in Pan-Cancer and Its Potential roles in Clear Renal Cell Carcinoma
Article Snippet: .. RNA isolation and quantitative real time polymerasechain reaction (qRT‐PCR) We extracted total RNA from cell lines using TRIzol Reagent (Invitrogen, USA), and then utilized PrimeScriptTM RT reagent Kit (EZBioscience, China) for reverse transcription to produce cDNA. qRT-PCR was nished using the SYBR Green PCR reagent (EZBioscience, China). ..

Article Title: A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder.
Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (RTqPCR): Total RNA of the synovial fibroblasts was isolated using an RNA Purification Kit (EZBioscience, USA) and transcribed into cDNA with a PrimeScript RT reagent kit (EZBioscience, USA). .. RT-qPCR analyses were then performed using a LightCycler 480 RealTime PCR System (Roche, Basel, Switzerland) with a SYBR Premix Ex Taq (Accurate Biology, China).

Purification:

Article Title: A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder
Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (RT-qPCR): Total RNA of the synovial fibroblasts was isolated using an RNA Purification Kit (EZBioscience, USA) and transcribed into cDNA with a PrimeScript RT reagent kit (EZBioscience, USA). .. RT-qPCR analyses were then performed using a LightCycler 480 RealTime PCR System (Roche, Basel, Switzerland) with a SYBR Premix Ex Taq (Accurate Biology, China).

Article Title: A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder.
Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (RTqPCR): Total RNA of the synovial fibroblasts was isolated using an RNA Purification Kit (EZBioscience, USA) and transcribed into cDNA with a PrimeScript RT reagent kit (EZBioscience, USA). .. RT-qPCR analyses were then performed using a LightCycler 480 RealTime PCR System (Roche, Basel, Switzerland) with a SYBR Premix Ex Taq (Accurate Biology, China).

Quantitative RT-PCR:

Article Title: The Oncogenic and Prognostic Significance of NSUN5 in Pan-Cancer and Its Potential roles in Clear Renal Cell Carcinoma
Article Snippet: .. RNA isolation and quantitative real time polymerasechain reaction (qRT‐PCR) We extracted total RNA from cell lines using TRIzol Reagent (Invitrogen, USA), and then utilized PrimeScriptTM RT reagent Kit (EZBioscience, China) for reverse transcription to produce cDNA. qRT-PCR was nished using the SYBR Green PCR reagent (EZBioscience, China). ..

Article Title: Proline-driven metabolic reprogramming promotes skeletal muscle hypertrophy and oxidative myofiber specification in porcine offspring: a stage-optimized maternal nutritional intervention.
Article Snippet: .. After reverse transcription using PrimerScript RT reagent Kit (EZBioscience, Guangzhou, China), RT-qPCR was performed to analyze the expression levels of related genes on a QuantStudio 6 RealTime PCR System (Thermo Fisher, Waltham, USA). ..

SYBR Green Assay:

Article Title: The Oncogenic and Prognostic Significance of NSUN5 in Pan-Cancer and Its Potential roles in Clear Renal Cell Carcinoma
Article Snippet: .. RNA isolation and quantitative real time polymerasechain reaction (qRT‐PCR) We extracted total RNA from cell lines using TRIzol Reagent (Invitrogen, USA), and then utilized PrimeScriptTM RT reagent Kit (EZBioscience, China) for reverse transcription to produce cDNA. qRT-PCR was nished using the SYBR Green PCR reagent (EZBioscience, China). ..

Article Title: Machine learning-derived natural killer cell signature predicts prognosis and therapeutic response in clear cell renal cell carcinoma.
Article Snippet: Background: Natural killer cells, interconnected with patient prognosis and treatment response, play a pivotal role in the tumor immune microenvironment and may serve as potential novel predictive biomarkers for renal

RNA Extraction:

Article Title: Machine learning-derived natural killer cell signature predicts prognosis and therapeutic response in clear cell renal cell carcinoma.
Article Snippet: Background: Natural killer cells, interconnected with patient prognosis and treatment response, play a pivotal role in the tumor immune microenvironment and may serve as potential novel predictive biomarkers for renal

Real-time Polymerase Chain Reaction:

Article Title: Machine learning-derived natural killer cell signature predicts prognosis and therapeutic response in clear cell renal cell carcinoma.
Article Snippet: Background: Natural killer cells, interconnected with patient prognosis and treatment response, play a pivotal role in the tumor immune microenvironment and may serve as potential novel predictive biomarkers for renal

Expressing:

Article Title: Proline-driven metabolic reprogramming promotes skeletal muscle hypertrophy and oxidative myofiber specification in porcine offspring: a stage-optimized maternal nutritional intervention.
Article Snippet: .. After reverse transcription using PrimerScript RT reagent Kit (EZBioscience, Guangzhou, China), RT-qPCR was performed to analyze the expression levels of related genes on a QuantStudio 6 RealTime PCR System (Thermo Fisher, Waltham, USA). ..

other:

Article Title: Tri-Layer Citrate-Based Hydroxyapatite Composite Scaffold Promoting Osteogenesis and Gingival Tissue Regeneration for Periodontal Bone Defect Repair.
Article Snippet: Periodontal bone defect (PBD) treatment involving oral soft and hard tissues is complicated and high requirements for regenerated materials.. Besides osteogenic effects, the materials are also required to have the function of barrier soft tissues and promote wound healing.. Citrate is reported to promote bone formation through enhanced osteoinductivity and facilitate wound healing by enabling phased angiogenesis.



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