taqman one-step rt-qpcr kit (Solarbio Inc)
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Solarbio Inc
taqman one-step rt-qpcr kit
Taqman One Step Rt Qpcr Kit, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+kit/primescript++rt+master+mix+kit/pmc09459177-56-9-13
Average 90 stars, based on 1 article reviews
Taqman One Step Rt Qpcr Kit, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+kit/primescript++rt+master+mix+kit/pmc09459177-56-9-13
Average 90 stars, based on 1 article reviews
taqman one-step rt-qpcr kit - by Bioz Stars,
2026-09
90/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). Isolation:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). RNA Extraction:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). Reverse Transcription Polymerase Chain Reaction:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). Quantitative RT-PCR:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). Expressing:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). Amplification:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). Reverse Transcription:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). Polymerase Chain Reaction:Article Title: Adaptive resistance in tumors to anti-PD-1 therapy through re-immunosuppression by upregulation of GPNMB expression. Article Snippet: Acquired resistance to the antitumor activity of antibodies targeting the programmed death 1 (PD-1): programmed death ligand 1 (PD-L1) immune checkpoint in various types of cancers has increasingly been observed during treatment.. To gain insight into the molecular mechanism underlying anti-PD-1 therapy resistance, we developed a mouse MC38 colon adenocarcinoma cell line that was made resistant to anti-PD-1 treatment through repeated in vivo selection.. We compared the transcriptomic profiles of anti-PD-1 therapy-resistant and -sensitive tumors using RNA sequencing analysis. Article Title: Endothelial cell-specific molecule 1 (ESM1) promoted by transcription factor SPI1 acts as an oncogene to modulate the malignant phenotype of endometrial cancer Article Snippet: Thereafter, qRT-PCR amplification was carried out in ABI7500 instrument (Applied Biosystems, USA) with the help of universal RT-PCR kit (RP1100; Solarbio). Article Title: Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation. Article Snippet: Real-Time Quantitative PCR Total RNA was isolated from macrophages, SVCs, BMDMs, or human WATs using the Total RNA Extraction Kit (Solarbio, Beijing, China) and reverse transcribed into cDNA using the One Step SuperRT-PCR Mix Kit (Solarbio). Article Title: Rejuvenation of BMSCs senescence by pharmacological enhancement of TFEB-mediated autophagy alleviates aged-related bone loss and extends lifespan in middle aged mice Article Snippet: 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche, Mannhein, Germany) according to the manufacturer’s instructions. Article Title: ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy. Article Snippet: Ferroptosis is a newly discovered non-apoptotic cell death form but its therapeutic efficacy triggered by traditional iron-based nanomaterials or classic drug inducers has been far from satisfactory due to the high glutathione (GSH) level in cancer cells and insufficient lipid peroxide production.. Here we reported a ferroptosis/apoptosis combinational therapy by depleting GSH and downregulating GPX4 to disrupt redox homeostasis and amplify ferroptosis-related oxidation effect.. In this study, we developed reactive oxygen species (ROS)-responsive serum-resistant nanoparticles with thioketal-crosslinked fluorinated polyethyleneimine 1.8K ( TK PF) as the core, which were wrapped with hyaluronic acid (HA) as the shell ( TK PFH NP) to co-deliver shGPX4 and shMTHFD2 plasmids for cancer treatment. Article Title: Circ_0007099 upregulates GNG7 to function as a tumor inhibitor in gastric carcinoma by interacting with miR-425-3p Article Snippet: Subsequently, a RevertAid First Strand cDNA Synthesis Kit and TaqMan One-Step RT-qPCR Kit (Solarbio) were used for reverse transcription and PCR detection according to the manufacturer’s guidelines. Article Title: Proteomic and Phenotypic Studies of Mycoplasma pneumoniae Revealed Macrolide-Resistant Mutation (A2063G) Associated Changes in Protein Composition and Pathogenicity of Type I Strains Article Snippet: Total RNA of the cells were extracted with TRIzol regent (Invitrogen), the extracted RNA was reverse transcribed (Universal RT-PCR kit, Solarbio) and detected by fluorescent quantitative PCR method (UltraSYBR RT-qPCR kit, CWbio). Article Title: BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis Article Snippet: Two microgram of total RNA was reverse transcribed to single strand cDNA using universal RT-PCR Kit (RP1100; Solarbio). |