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Macrogen primer3 algorithm
Primer3 Algorithm, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer3+algorithm/primer3+software/pm39085355-80-9-14
Average 90 stars, based on 1 article reviews
primer3 algorithm - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Quantitative RT-PCR:

Article Title: Investigation of ITGB3 Heterogeneity to Overcome Trastuzumab Resistance in HER2-Positive Breast Cancer.
Article Snippet: .. The melting curve analysis included a denaturation phase at 95 ◦C for 15 s, annealing at 60 ◦C for 1 min, and final elongation at 95 ◦C for 15 s. Primers used for RT-qPCR were custom-designed using the Primer3 software and obtained from Macrogen (Supplementary Table S1). ..

Article Title: Investigation of ITGB3 Heterogeneity to Overcome Trastuzumab Resistance in HER2-Positive Breast Cancer
Article Snippet: .. The melting curve analysis included a denaturation phase at 95 °C for 15 s, annealing at 60 °C for 1 min, and final elongation at 95 °C for 15 s. Primers used for RT-qPCR were custom-designed using the Primer3 software and obtained from Macrogen ( ). ..

Software:

Article Title: Investigation of ITGB3 Heterogeneity to Overcome Trastuzumab Resistance in HER2-Positive Breast Cancer.
Article Snippet: .. The melting curve analysis included a denaturation phase at 95 ◦C for 15 s, annealing at 60 ◦C for 1 min, and final elongation at 95 ◦C for 15 s. Primers used for RT-qPCR were custom-designed using the Primer3 software and obtained from Macrogen (Supplementary Table S1). ..

Article Title: Rapid, economical single-nucleotide polymorphism and microsatellite discovery based on de novo assembly of a reduced representation genome in a non-model organism: a case study of Atlantic cod Gadus morhua.
Article Snippet: *Beaufort Genetics Research Programme, School of Biological, Earth and Environmental Sciences/Aquauculture and Fisheries Development Centre, University College Cork, Distillery Fields, North Mall, Cork, Ireland, †School of Biology and Environmental Science, UCD Science Education and Research Centre – West, University College Dublin, Dublin 4, Ireland, §Department of Biological Sciences, 202E Mills Godwin Bldg., Old Dominion University, Norfolk, VA, 23529, U.S.A. and ‖Carna Research Station, Ryan Institute, National University of Ireland, Galway, Carna, Connemara, Co., Galway, Ireland

Article Title: Real time PCR detection of common CYP2D6 genetic variants and its application in a Karen population study.
Article Snippet: .. Optimal primer sequences of PCR-reamplified products were selected by Primer3 software version 0.4.0 and were then synthesized by Macrogen Inc. (Korea). .. Variant-specific primer and probe sets of real-time PCR were designed and supplied directly by Applied Biosystems (Thermal Fisher Scientific, Inc.).

Article Title: Investigation of ITGB3 Heterogeneity to Overcome Trastuzumab Resistance in HER2-Positive Breast Cancer
Article Snippet: .. The melting curve analysis included a denaturation phase at 95 °C for 15 s, annealing at 60 °C for 1 min, and final elongation at 95 °C for 15 s. Primers used for RT-qPCR were custom-designed using the Primer3 software and obtained from Macrogen ( ). ..

Amplification:

Article Title: Rapid, economical single-nucleotide polymorphism and microsatellite discovery based on de novo assembly of a reduced representation genome in a non-model organism: a case study of Atlantic cod Gadus morhua.
Article Snippet: *Beaufort Genetics Research Programme, School of Biological, Earth and Environmental Sciences/Aquauculture and Fisheries Development Centre, University College Cork, Distillery Fields, North Mall, Cork, Ireland, †School of Biology and Environmental Science, UCD Science Education and Research Centre – West, University College Dublin, Dublin 4, Ireland, §Department of Biological Sciences, 202E Mills Godwin Bldg., Old Dominion University, Norfolk, VA, 23529, U.S.A. and ‖Carna Research Station, Ryan Institute, National University of Ireland, Galway, Carna, Connemara, Co., Galway, Ireland

Article Title: Low-level brain somatic mutations in exonic regions are collectively implicated in autism with germline mutations in autism risk genes.
Article Snippet: .. Targeted amplicon validation sequencing Primers were designed with the Primer3 algorithm and synthesized by Macrogen (Seoul, Korea). .. Amplicons were prepared by two-step PCR using Illumina TruSeq adapters.

Sequencing:

Article Title: Rapid, economical single-nucleotide polymorphism and microsatellite discovery based on de novo assembly of a reduced representation genome in a non-model organism: a case study of Atlantic cod Gadus morhua.
Article Snippet: *Beaufort Genetics Research Programme, School of Biological, Earth and Environmental Sciences/Aquauculture and Fisheries Development Centre, University College Cork, Distillery Fields, North Mall, Cork, Ireland, †School of Biology and Environmental Science, UCD Science Education and Research Centre – West, University College Dublin, Dublin 4, Ireland, §Department of Biological Sciences, 202E Mills Godwin Bldg., Old Dominion University, Norfolk, VA, 23529, U.S.A. and ‖Carna Research Station, Ryan Institute, National University of Ireland, Galway, Carna, Connemara, Co., Galway, Ireland

Article Title: Low-level brain somatic mutations in exonic regions are collectively implicated in autism with germline mutations in autism risk genes.
Article Snippet: .. Targeted amplicon validation sequencing Primers were designed with the Primer3 algorithm and synthesized by Macrogen (Seoul, Korea). .. Amplicons were prepared by two-step PCR using Illumina TruSeq adapters.

Biomarker Discovery:

Article Title: Low-level brain somatic mutations in exonic regions are collectively implicated in autism with germline mutations in autism risk genes.
Article Snippet: .. Targeted amplicon validation sequencing Primers were designed with the Primer3 algorithm and synthesized by Macrogen (Seoul, Korea). .. Amplicons were prepared by two-step PCR using Illumina TruSeq adapters.

Synthesized:

Article Title: Low-level brain somatic mutations in exonic regions are collectively implicated in autism with germline mutations in autism risk genes.
Article Snippet: .. Targeted amplicon validation sequencing Primers were designed with the Primer3 algorithm and synthesized by Macrogen (Seoul, Korea). .. Amplicons were prepared by two-step PCR using Illumina TruSeq adapters.

Article Title: Real time PCR detection of common CYP2D6 genetic variants and its application in a Karen population study.
Article Snippet: .. Optimal primer sequences of PCR-reamplified products were selected by Primer3 software version 0.4.0 and were then synthesized by Macrogen Inc. (Korea). .. Variant-specific primer and probe sets of real-time PCR were designed and supplied directly by Applied Biosystems (Thermal Fisher Scientific, Inc.).

Article Title: Production and Molecular Identification of Interspecific Hybrids between Phaius mishmensis (Lindl. and Paxton) Rchb. f. and Phaius tankervilliae (Banks) Blume
Article Snippet: Representative sequences of each species were utilized for specific SCAR primer design using website IDTDNA (https://sg.idtdna.com/site, accessed on 16 February 2021). .. The primers were rechecked by the Primer 3 program (http://bioinfo.ut.ee/primer3--0.4/primer3/, accessed on 16 February 2021) and synthesized by Macrogen Company (Seoul, Korea). ..

Polymerase Chain Reaction:

Article Title: Real time PCR detection of common CYP2D6 genetic variants and its application in a Karen population study.
Article Snippet: .. Optimal primer sequences of PCR-reamplified products were selected by Primer3 software version 0.4.0 and were then synthesized by Macrogen Inc. (Korea). .. Variant-specific primer and probe sets of real-time PCR were designed and supplied directly by Applied Biosystems (Thermal Fisher Scientific, Inc.).

other:

Article Title: Bioinformatics Analyses of Serine Acetyltransferase (SAT) Gene Family in Rice (Oryza sativa) and their Expressions under Salt Stress
Article Snippet: Primers used in the study (Table 1) were designed using Primer3. v. 0.4.0 program (Untergasser et al. 2012) (http://bioinfo.ut.ee/primer3-0.4.0/) and synthesized by Macrogen Crop. (Amsterdam, The Netherlands).



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