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super-script ii reverse transcriptase kit with an oligo(dt) 12–18 primer  (Thermo Fisher)


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    Thermo Fisher super-script ii reverse transcriptase kit with an oligo(dt) 12–18 primer
    Super Script Ii Reverse Transcriptase Kit With An Oligo(dt) 12–18 Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer+script+reverse+transcriptase+kit/pmc02742303-91-27-36
    Average 90 stars, based on 1 article reviews
    super-script ii reverse transcriptase kit with an oligo(dt) 12–18 primer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Dietary oleic acid intake increases the proportion of type 1 and 2X muscle fibers in mice
    Article Snippet: EDL and soleus muscle tissues were pulverized under liquid nitrogen, then total RNA was extracted from them using the ISOGENII kit (NIPPON GENE, Tokyo, Japan) and reverse transcribed using SuperScript III Reverse Transcriptase (Invitrogen, Grand Island, NY, USA) and Oligo d(T)12–18 primers (Applied Biosystems, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: GEF-H1 Transduces FcεRI Signaling in Mast Cells to Activate RhoA and Focal Adhesion Formation during Exocytosis.
    Article Snippet: RNA was precipitated from the top aqueous phase by adding an equal volume of isopropanol, washed in 70% ethanol, and 5 μg was used to synthesize cDNA using 100 units SuperScriptTM II Reverse Transcriptase (Invitrogen, Waltham, MA, USA) and 0.5 μg Oligo (dT)12–18 primer (Invitrogen, Waltham, MA, USA) in a 20 μL reaction.

    Article Title: Method of reducing antigenic drift or reassortment of viruses in a host animal using α interferon
    Article Snippet: Two μg of amplified RNA, 1 μl dT12-18 primer (SEQ ID NO: 3) (1 μg per μl, Invitrogen), 2.6 μl random hexanucleotides (3 μg per μl, Invitrogen) and 1 μl anti-RNAse (Ambion) were combined in a reaction volume of 15.5 μl and incubated for 10 min at 70° C. Reverse transcription was for 2 h at 42° C. in a 30 μl reaction containing annealed RNA template, first strand buffer, 500 mM each dATP, dCTP, dGTP, 300 μM dTTP, 200 μM aminoallyl-dUTP (Sigma), 10 mM DTT, 12.7 U per μl Superscript II.

    Article Title: Cytotoxic T-Lymphocyte-Associated Protein 4 Fused to a Modified Fragment of IgG1 Reduces Muscle Fiber Damage in a Model of Duchenne Muscular Dystrophy by Attenuating Proinflammatory Gene Expression in Myeloid Lineage Cells.
    Article Snippet: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 ajp.amjpathol.org 63 64 65 66 67 68 69 70 71 72 73 74 Cytotoxic T-LymphocyteeAssociated Protein 4 75 76 Fused to a Modified Fragment of IgG1 Reduces 77 78 79 80 81 82 83 84 85 86 Muscle Fiber Damage in a Model of Duchenne Muscular Dystrophy by Attenuating Proinflammatory Gene Expression in Myeloid Lineage Cells Michelle Wehling-Henricks,* Su-yin Kok,* Haley Gamboa,* Pranav Kannan,*y Connor Thomas,* Ivan Flores,y Steven S. Welc,zx and James G. Tidball*y{ 87 88 89 90 From the Department of Integrative Biology and Physiology,* the Molecular, Cellular and Integrative Physiology Program,y and the Department of Pathology and Laboratory Medicine,{ David Geffen School of Medicine at UCLA, University of California, Los Angeles, California; and the Department of Anatomy, Cell Biology and Physiologyz and the Indiana Center for Musculoskeletal Health,x Indiana University School of Medicine, Indianapolis, Indiana 91 92 93 Accepted for publication

    Article Title: The RIDD activity of C. elegans IRE1 modifies neuroendocrine signaling in anticipation of environment stress to ensure survival
    Article Snippet: 200ng of extracted RNA was retro-transcribed to cDNA by using oligo (dT)12-18 (Invitrogen) and SuperScript II reverse transcriptase (Invitrogen).

    Extraction:

    Article Title: Reticular adhesions are assembled at flat clathrin lattices and opposed by active integrin α5β1
    Article Snippet: .. Total RNA was extracted from cells using TRIzol reagent with an additional acidic phenol (pH 5.4) extraction step to remove genomic DNA contamination. cDNA synthesis from 1 μg total RNA was carried out using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific Scientific) and oligo dT12–18 (Life Technologies). ..

    cDNA Synthesis:

    Article Title: Reticular adhesions are assembled at flat clathrin lattices and opposed by active integrin α5β1
    Article Snippet: .. Total RNA was extracted from cells using TRIzol reagent with an additional acidic phenol (pH 5.4) extraction step to remove genomic DNA contamination. cDNA synthesis from 1 μg total RNA was carried out using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific Scientific) and oligo dT12–18 (Life Technologies). ..

    Reverse Transcription:

    Article Title: Reticular adhesions are assembled at flat clathrin lattices and opposed by active integrin α5β1
    Article Snippet: .. Total RNA was extracted from cells using TRIzol reagent with an additional acidic phenol (pH 5.4) extraction step to remove genomic DNA contamination. cDNA synthesis from 1 μg total RNA was carried out using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific Scientific) and oligo dT12–18 (Life Technologies). ..

    Article Title: Preferential lipolysis of DGAT1 over DGAT2 generated triacylglycerol in Huh7 hepatocytes.
    Article Snippet: .. First-strand cDNA was synthesized from 2 μg of total RNA using Superscript ІІІ reverse transcriptase (Invitrogen) primed by oligo (dt)12–18 (Invitrogen) and random primers (Invitrogen). .. Real-time quantitative qPCR was performed with Power SYBR® Green PCR Master Mix (Life Technologies, UK) using the StepOnePlus real-time PCR system (Applied Biosystems, Canada).

    Synthesized:

    Article Title: Preferential lipolysis of DGAT1 over DGAT2 generated triacylglycerol in Huh7 hepatocytes.
    Article Snippet: .. First-strand cDNA was synthesized from 2 μg of total RNA using Superscript ІІІ reverse transcriptase (Invitrogen) primed by oligo (dt)12–18 (Invitrogen) and random primers (Invitrogen). .. Real-time quantitative qPCR was performed with Power SYBR® Green PCR Master Mix (Life Technologies, UK) using the StepOnePlus real-time PCR system (Applied Biosystems, Canada).



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    Thermo Fisher super-script ii reverse transcriptase kit with an oligo(dt) 12–18 primer
    Super Script Ii Reverse Transcriptase Kit With An Oligo(dt) 12–18 Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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