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40% polypep solution  (Millipore)


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    Structured Review

    Millipore 40% polypep solution
    40% Polypep Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polypep+solution/low+viscosity+polypeptide/pmc07999916-159-17-19
    Average 90 stars, based on 1 article reviews
    40% polypep solution - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Integrated coastal monitoring of a gas processing plant using native and caged mussels.
    Article Snippet: Contents lists available at SciVerse ScienceDirect Science of the Total Environment j ourna l homepage: www.e lsev ie r .com/ locate /sc i totenv Integrated coastal monitoring of a gas processing plant using native and caged mussels Steven Brooks a,⁎, Christopher Harman a, Manu Soto b, Ibon Cancio b, Tormod Glette c, Ionan Marigómez b a Norwegian Institute for Water Research (NIVA), Gaustadalléen 21, NO-0349 Oslo, Norway b CBET Res Grp, R&D Centre for Experimental Marine Biology and Biotechnology (PIE), Univ Basque Country, Areatza Z/G, Plentzia-Bizkaia, E-48620 Basque Country, Spain c Det Norske Veritas (DNV), Veritasveien 1, 1363 Høvik, Norway ⁎ Corresponding author.. Tel.. :+4722185100,+4792696 E-mail address: sbr@niva.no (S. Brooks).

    Article Title: Differences in copper bioaccumulation and biological responses in three Mytilus species.
    Article Snippet: .. The incubation medium consisted of 20 mg naphthol ASBI-Nacetyl- -d glucosaminide (Sigma, N 4006) dissolved in 2.5 mL 2-methoxyethanol (Merck, 859), and made up to 50 mL with 0.1 M citrate buffer (pH 4.5) containing 2.5% NaCl and 3.5 g low viscosity polypeptide (Sigma, P5115) to act as a section stabiliser. .. Sections were incubated in this medium for 20 min at 37 ◦C, rinsed in a saline solution (3.0% NaCl) at 37 ◦C for 2 min and then transferred to 0.1 M phosphate buffer (pH 7.4) containing 1 mg/mL diazonium dye Fast Violet B salt (Sigma, F1631), at RT for 10 min.

    Article Title: Integrated biomarker assessment of the effects exerted by treated produced water from an onshore natural gas processing plant in the North Sea on the mussel Mytilus edulis.
    Article Snippet: 0025-326X/$ see front matter 2010 Elsevier Ltd. A doi:10.1016/j.marpolbul.2010.10.007 ⇑ Corresponding author.. Tel.. : +47 22185100; mo 22185200.

    Article Title: Time-course study of the early lysosomal responses to pollutants in mussel digestive cells using acid phosphatase as lysosomal marker enzyme.
    Article Snippet: Article history: Lysosomal biomarkers are e Received 22 October 2008 Received in revised form 2 January 2009 Accepted 4 January 2009 Available online 13 January 2009

    Article Title: Influence of season-depending ecological variables on biomarker baseline levels in mussels (Mytilus trossulus) from two Baltic Sea subregions.
    Article Snippet: .. Following this treatment, sections were transferred to the substrate incubation medium for the demonstration of Hex activity, consisting of 20 mg naphthol AS BI N acetyl b D glucosaminide (Sigma, N 4006) dissolved in 2.5 ml of 2 methoxyethanol (Merck, 859), and made up to 50 ml with 0.1 M citrate buffer (pH 4.5) containing 2.5% NaCl and 3.5 g of low viscosity polypeptide (Sigma, P5115) to act as a section stabiliser. .. Sections were incubated in this medium for 20 min at 37 °C, rinsed in a saline solution (3.0% NaCl) at 37C for 2 min and then transferred to 0.1 M phosphate buffer (pH 7.4) containing 1 mg/ml of diazonium dye Fast Violet B salt (Sigma, F1631), at RT for 10 min.

    Viscosity:

    Article Title: Integrated coastal monitoring of a gas processing plant using native and caged mussels.
    Article Snippet: Contents lists available at SciVerse ScienceDirect Science of the Total Environment j ourna l homepage: www.e lsev ie r .com/ locate /sc i totenv Integrated coastal monitoring of a gas processing plant using native and caged mussels Steven Brooks a,⁎, Christopher Harman a, Manu Soto b, Ibon Cancio b, Tormod Glette c, Ionan Marigómez b a Norwegian Institute for Water Research (NIVA), Gaustadalléen 21, NO-0349 Oslo, Norway b CBET Res Grp, R&D Centre for Experimental Marine Biology and Biotechnology (PIE), Univ Basque Country, Areatza Z/G, Plentzia-Bizkaia, E-48620 Basque Country, Spain c Det Norske Veritas (DNV), Veritasveien 1, 1363 Høvik, Norway ⁎ Corresponding author.. Tel.. :+4722185100,+4792696 E-mail address: sbr@niva.no (S. Brooks).

    Article Title: Differences in copper bioaccumulation and biological responses in three Mytilus species.
    Article Snippet: .. The incubation medium consisted of 20 mg naphthol ASBI-Nacetyl- -d glucosaminide (Sigma, N 4006) dissolved in 2.5 mL 2-methoxyethanol (Merck, 859), and made up to 50 mL with 0.1 M citrate buffer (pH 4.5) containing 2.5% NaCl and 3.5 g low viscosity polypeptide (Sigma, P5115) to act as a section stabiliser. .. Sections were incubated in this medium for 20 min at 37 ◦C, rinsed in a saline solution (3.0% NaCl) at 37 ◦C for 2 min and then transferred to 0.1 M phosphate buffer (pH 7.4) containing 1 mg/mL diazonium dye Fast Violet B salt (Sigma, F1631), at RT for 10 min.

    Article Title: Integrated biomarker assessment of the effects exerted by treated produced water from an onshore natural gas processing plant in the North Sea on the mussel Mytilus edulis.
    Article Snippet: 0025-326X/$ see front matter 2010 Elsevier Ltd. A doi:10.1016/j.marpolbul.2010.10.007 ⇑ Corresponding author.. Tel.. : +47 22185100; mo 22185200.

    Article Title: Time-course study of the early lysosomal responses to pollutants in mussel digestive cells using acid phosphatase as lysosomal marker enzyme.
    Article Snippet: Article history: Lysosomal biomarkers are e Received 22 October 2008 Received in revised form 2 January 2009 Accepted 4 January 2009 Available online 13 January 2009

    Article Title: Influence of season-depending ecological variables on biomarker baseline levels in mussels (Mytilus trossulus) from two Baltic Sea subregions.
    Article Snippet: .. Following this treatment, sections were transferred to the substrate incubation medium for the demonstration of Hex activity, consisting of 20 mg naphthol AS BI N acetyl b D glucosaminide (Sigma, N 4006) dissolved in 2.5 ml of 2 methoxyethanol (Merck, 859), and made up to 50 ml with 0.1 M citrate buffer (pH 4.5) containing 2.5% NaCl and 3.5 g of low viscosity polypeptide (Sigma, P5115) to act as a section stabiliser. .. Sections were incubated in this medium for 20 min at 37 °C, rinsed in a saline solution (3.0% NaCl) at 37C for 2 min and then transferred to 0.1 M phosphate buffer (pH 7.4) containing 1 mg/ml of diazonium dye Fast Violet B salt (Sigma, F1631), at RT for 10 min.

    other:

    Article Title: Health and growth performance of the blue mussel (Mytilus edulis L.) from two hanging cultivation sites in the German Bight: a nearshore—offshore comparison
    Article Snippet: The health of cultivated blue mussels from an offshore and a nearshore sites was compared by assessing lysosomal membrane stability (LMS) and accumulations of lipofuscin and neutral lipids in the digestive glands.. In addition, the condition index, parasite infestation rates, growth performance and energy status of the mussels were assessed during 10 months of sampling.. Abiotic and biotic site characteristics were investigated by water analysis for each sampling cycle.

    Activity Assay:

    Article Title: Time-course study of the early lysosomal responses to pollutants in mussel digestive cells using acid phosphatase as lysosomal marker enzyme.
    Article Snippet: Article history: Lysosomal biomarkers are e Received 22 October 2008 Received in revised form 2 January 2009 Accepted 4 January 2009 Available online 13 January 2009

    Article Title: Influence of season-depending ecological variables on biomarker baseline levels in mussels (Mytilus trossulus) from two Baltic Sea subregions.
    Article Snippet: .. Following this treatment, sections were transferred to the substrate incubation medium for the demonstration of Hex activity, consisting of 20 mg naphthol AS BI N acetyl b D glucosaminide (Sigma, N 4006) dissolved in 2.5 ml of 2 methoxyethanol (Merck, 859), and made up to 50 ml with 0.1 M citrate buffer (pH 4.5) containing 2.5% NaCl and 3.5 g of low viscosity polypeptide (Sigma, P5115) to act as a section stabiliser. .. Sections were incubated in this medium for 20 min at 37 °C, rinsed in a saline solution (3.0% NaCl) at 37C for 2 min and then transferred to 0.1 M phosphate buffer (pH 7.4) containing 1 mg/ml of diazonium dye Fast Violet B salt (Sigma, F1631), at RT for 10 min.

    Article Title: The influence of intervertebral disc overloading on nociceptor calcium flickering
    Article Snippet: .. Dead cells loss the enzyme activity 36 h after cell death and is therefore not stained into the purple color, while their nuclei can be detected by ethidium homodimer-1 staining.20 LDH staining method was performed based on a formerly described protocol.21 Briefly, the sections were firstly stained in a 40% polypep solution (P5115, Sigma, Buchs, CH) containing 54 mM lactic acid (69 771, Fluka, Buchs, CH) and 1.75 mg/mL β-nicotinamide adenine dinucleotide hydrate (43410, Fluka, Buchs, CH). .. Dead cells loss the enzyme activity 36 h after cell death and is therefore not stained into the purple color, while their nuclei can be detected by ethidium homodimer-1 staining.20 LDH staining method was performed based on a formerly described protocol.21 Briefly, the sections were firstly stained in a 40% polypep solution (P5115, Sigma, Buchs, CH) containing 54 mM lactic acid (69 771, Fluka, Buchs, CH) and 1.75 mg/mL β-nicotinamide adenine dinucleotide hydrate (43410, Fluka, Buchs, CH).

    Staining:

    Article Title: The influence of intervertebral disc overloading on nociceptor calcium flickering
    Article Snippet: .. Dead cells loss the enzyme activity 36 h after cell death and is therefore not stained into the purple color, while their nuclei can be detected by ethidium homodimer-1 staining.20 LDH staining method was performed based on a formerly described protocol.21 Briefly, the sections were firstly stained in a 40% polypep solution (P5115, Sigma, Buchs, CH) containing 54 mM lactic acid (69 771, Fluka, Buchs, CH) and 1.75 mg/mL β-nicotinamide adenine dinucleotide hydrate (43410, Fluka, Buchs, CH). .. Dead cells loss the enzyme activity 36 h after cell death and is therefore not stained into the purple color, while their nuclei can be detected by ethidium homodimer-1 staining.20 LDH staining method was performed based on a formerly described protocol.21 Briefly, the sections were firstly stained in a 40% polypep solution (P5115, Sigma, Buchs, CH) containing 54 mM lactic acid (69 771, Fluka, Buchs, CH) and 1.75 mg/mL β-nicotinamide adenine dinucleotide hydrate (43410, Fluka, Buchs, CH).



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