polya selection module (New England Biolabs)
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polya selection module
Polya Selection Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polya+selection+module/NEBNext+Ultra+Ligation+Module/pmc10141529-337-12-15
Average 95 stars, based on 449 article reviews
Polya Selection Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polya+selection+module/NEBNext+Ultra+Ligation+Module/pmc10141529-337-12-15
Average 95 stars, based on 449 article reviews
polya selection module - by Bioz Stars,
2026-09
95/100 stars
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Chromatin Immunoprecipitation:Article Title: Mechanism insights into the regulation of the LuxS/AI-2 quorum sensing system on the formation of viable but nonculturable state in biofilm cells of beer-spoilage Lactiplantibacillus plantarum. Article Snippet: Bound DNA was eluted with 300 lL elution buffer (100 mM NaHCO3 (Macklin, #S837271), 1 % SDS) and treated sequentially with RNase A (8 lg/mL, ThermoFisher #EN0531, 65 °C, 6 h) and proteinase K (345 lg/mL, Macklin #39450–01-6, 45 °C, overnight). .. ChIP and input DNA were end-repaired/dA-tailed (NEB #E7442), ligated to Amplification:Article Title: Mechanism insights into the regulation of the LuxS/AI-2 quorum sensing system on the formation of viable but nonculturable state in biofilm cells of beer-spoilage Lactiplantibacillus plantarum. Article Snippet: Bound DNA was eluted with 300 lL elution buffer (100 mM NaHCO3 (Macklin, #S837271), 1 % SDS) and treated sequentially with RNase A (8 lg/mL, ThermoFisher #EN0531, 65 °C, 6 h) and proteinase K (345 lg/mL, Macklin #39450–01-6, 45 °C, overnight). .. ChIP and input DNA were end-repaired/dA-tailed (NEB #E7442), ligated to Sequencing:Article Title: Posttranscriptional 3′-Terminal Modifications of Escherichia coli RNA Fragments Evolved for Diversity Boosting Article Snippet: Amplicons were then purified using AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). .. Sequencing libraries were prepared using the Native Barcoding Kit 96 V14 (SQK-NBD114.96) protocol, with modified incubation times for enzymatic reactions: the DNA repair step using NEBNext ® UltraTM II End Repair/dA-Tailing Article Title: Bryophytes hold a larger gene family space than vascular plants. Article Snippet: Transcriptome libraries were constructed with a TruSeq RNA Library Prep Kit v2 (Illumina) with an insert size of 200–400 bp, after polyA selection, and were sequenced for 150 bp paired-end reads on the MGI-SEQ or Illumina NovaSeq 6000 platform. .. For long-read sequencing, DNA fragments over 30 kb were selected for library construction, and the library was first end-repaired with Article Title: Bryophytes hold a larger gene family space than vascular plants Article Snippet: Transcriptome libraries were constructed with a TruSeq RNA Library Prep Kit v2 (Illumina) with an insert size of 200–400 bp, after polyA selection, and were sequenced for 150 bp paired-end reads on the MGI-SEQ or Illumina NovaSeq 6000 platform. .. For long-read sequencing, DNA fragments over 30 kb were selected for library construction, and the library was first end-repaired with Modification:Article Title: Posttranscriptional 3′-Terminal Modifications of Escherichia coli RNA Fragments Evolved for Diversity Boosting Article Snippet: Amplicons were then purified using AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). .. Sequencing libraries were prepared using the Native Barcoding Kit 96 V14 (SQK-NBD114.96) protocol, with modified incubation times for enzymatic reactions: the DNA repair step using NEBNext ® UltraTM II End Repair/dA-Tailing Incubation:Article Title: Posttranscriptional 3′-Terminal Modifications of Escherichia coli RNA Fragments Evolved for Diversity Boosting Article Snippet: Amplicons were then purified using AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). .. Sequencing libraries were prepared using the Native Barcoding Kit 96 V14 (SQK-NBD114.96) protocol, with modified incubation times for enzymatic reactions: the DNA repair step using NEBNext ® UltraTM II End Repair/dA-Tailing Ligation:Article Title: Posttranscriptional 3′-Terminal Modifications of Escherichia coli RNA Fragments Evolved for Diversity Boosting Article Snippet: Amplicons were then purified using AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). .. Sequencing libraries were prepared using the Native Barcoding Kit 96 V14 (SQK-NBD114.96) protocol, with modified incubation times for enzymatic reactions: the DNA repair step using NEBNext ® UltraTM II End Repair/dA-Tailing Article Title: Bryophytes hold a larger gene family space than vascular plants. Article Snippet: Transcriptome libraries were constructed with a TruSeq RNA Library Prep Kit v2 (Illumina) with an insert size of 200–400 bp, after polyA selection, and were sequenced for 150 bp paired-end reads on the MGI-SEQ or Illumina NovaSeq 6000 platform. .. For long-read sequencing, DNA fragments over 30 kb were selected for library construction, and the library was first end-repaired with Article Title: Bryophytes hold a larger gene family space than vascular plants Article Snippet: Transcriptome libraries were constructed with a TruSeq RNA Library Prep Kit v2 (Illumina) with an insert size of 200–400 bp, after polyA selection, and were sequenced for 150 bp paired-end reads on the MGI-SEQ or Illumina NovaSeq 6000 platform. .. For long-read sequencing, DNA fragments over 30 kb were selected for library construction, and the library was first end-repaired with Article Title: DNA construct for sequencing and method for preparing the same Article Snippet: End Repair/dA-Tailing Follow the NEBNext® UltraTM End Repair/dA-Tailing Module (NEB #E7442) protocol: Mix the following components in a sterile, nuclease-free tube: (green) End Prep Enzyme Mix—3.0 μl; (green) End Repair Reaction Buffer (10×)—6.5 μl; PCR amplicons from previous step—55.5 μl; Mix by pipetting, followed by a quick spin to collect all liquid from the sides of the tube. .. Place in a thermocycler, with the heated lid on, and run the following program: 30 minutes @ 20° C.; 30 minutes @ 65° C.; Hold at 4° C. Proceed directly to Formalin-fixed Paraffin-Embedded:Article Title: Bryophytes hold a larger gene family space than vascular plants. Article Snippet: Transcriptome libraries were constructed with a TruSeq RNA Library Prep Kit v2 (Illumina) with an insert size of 200–400 bp, after polyA selection, and were sequenced for 150 bp paired-end reads on the MGI-SEQ or Illumina NovaSeq 6000 platform. .. For long-read sequencing, DNA fragments over 30 kb were selected for library construction, and the library was first end-repaired with Article Title: Bryophytes hold a larger gene family space than vascular plants Article Snippet: Transcriptome libraries were constructed with a TruSeq RNA Library Prep Kit v2 (Illumina) with an insert size of 200–400 bp, after polyA selection, and were sequenced for 150 bp paired-end reads on the MGI-SEQ or Illumina NovaSeq 6000 platform. .. For long-read sequencing, DNA fragments over 30 kb were selected for library construction, and the library was first end-repaired with Spectrophotometry:Article Title: Differences in microbial composition of litter and water line biofilm of broiler farms as influenced by water quality history. Article Snippet: The yield and quality of the PCR product were then assessed using 1 % w/v agarose gel electrophoresis and subsequently purified with AMPure XP beads (Beckman Coulter, Brea, CA, USA). .. The cleaned amplicons were quantified using a NanoDrop One spectrophotometer (Thermo Scientific, Wilmington, DE, USA), end-prepped using the NEBNext Ultra II End Repair/dA-Tailing Article Title: Differences in microbial composition of litter and water line biofilm of broiler farms as influenced by water quality history Article Snippet: The yield and quality of the PCR product were then assessed using 1 % w/v agarose gel electrophoresis and subsequently purified with AMPure XP beads (Beckman Coulter, Brea, CA, USA). .. The cleaned amplicons were quantified using a NanoDrop One spectrophotometer (Thermo Scientific, Wilmington, DE, USA), end-prepped using the NEBNext Ultra II End Repair/dA-Tailing Concentration Assay:Article Title: DNA construct for sequencing and method for preparing the same Article Snippet: End Repair/dA-Tailing Follow the NEBNext® UltraTM End Repair/dA-Tailing Module (NEB #E7442) protocol: Mix the following components in a sterile, nuclease-free tube: (green) End Prep Enzyme Mix—3.0 μl; (green) End Repair Reaction Buffer (10×)—6.5 μl; PCR amplicons from previous step—55.5 μl; Mix by pipetting, followed by a quick spin to collect all liquid from the sides of the tube. .. Place in a thermocycler, with the heated lid on, and run the following program: 30 minutes @ 20° C.; 30 minutes @ 65° C.; Hold at 4° C. Proceed directly to |
