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tef1p cas9 cyc1t  (Addgene inc)


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    Structured Review

    Addgene inc tef1p cas9 cyc1t
    Tef1p Cas9 Cyc1t, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-4-10
    Average 93 stars, based on 127 article reviews
    tef1p cas9 cyc1t - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
    Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

    Cloning:

    Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
    Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

    Plasmid Preparation:

    Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
    Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: .. The operations are as follows: The codon-optimized Cas9 from Streptococcus pyogenes was obtained from the plasmid p414-TEF1p- Cas9-CYC1t (Addgene number: #43802) and used for expression in B . cinerea TB-31. ..

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..

    Construct:

    Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
    Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

    Polymerase Chain Reaction:

    Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
    Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

    Ligation:

    Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
    Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

    Transformation Assay:

    Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
    Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..

    Expressing:

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: .. The operations are as follows: The codon-optimized Cas9 from Streptococcus pyogenes was obtained from the plasmid p414-TEF1p- Cas9-CYC1t (Addgene number: #43802) and used for expression in B . cinerea TB-31. ..

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..

    other:


    CRISPR:

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..

    Selection:

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

    Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
    Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..



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