Review




Structured Review

Addgene inc p414
P414, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-0-10
Average 93 stars, based on 127 article reviews
p414 - by Bioz Stars, 2026-09
93/100 stars

Images

Related Articles

Clone Assay:

Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

Cloning:

Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

Plasmid Preparation:

Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
Article Snippet: .. The operations are as follows: The codon-optimized Cas9 from Streptococcus pyogenes was obtained from the plasmid p414-TEF1p- Cas9-CYC1t (Addgene number: #43802) and used for expression in B . cinerea TB-31. ..

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..

Construct:

Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

Polymerase Chain Reaction:

Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

Ligation:

Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

Transformation Assay:

Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the p414-TEF1p-Cas9-CYC1t using a seamless cloning kit (Sangon Biotech Co., Shanghai, China), and replaced the original SpCas9 gene, resulting in the plasmid pZB214. pZB211 that containing the fragment P ScSNR52 -DR was constructed by PCR with p426-SNR52p-gRNA.CAN1.Y-SUP4t (Addgene 43803) as template using primers 5′-phosphorylation 426P-F and 426P-R following ligation and transformation. ..

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..

Expressing:

Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
Article Snippet: .. The operations are as follows: The codon-optimized Cas9 from Streptococcus pyogenes was obtained from the plasmid p414-TEF1p- Cas9-CYC1t (Addgene number: #43802) and used for expression in B . cinerea TB-31. ..

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..

other:


CRISPR:

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..

Selection:

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. (DiCarlo et 23 al. 2013): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, 24 Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under 25 the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-26 418 or hygromycin selection. ..

Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing
Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a low-copy CEN6/ARS4 p414 plasmid (#43802, Addgene) with clonNat selection under a strong constitutive TEF1 promoter. gRNA was expressed under the SNR52 promoter from sequentially transformed high-copy 2μ gRNA expression plasmid p426 with G-418 or hygromycin selection. ..



Similar Products

96
Zymo Research zymopure plasmid miniprep kit
Zymopure Plasmid Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/ZymoPURE+Plasmid+Miniprep+Kit/custom%40d4208t%4042674247
Average 96 stars, based on 1 article reviews
zymopure plasmid miniprep kit - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

93
Sino Biological rat mlc1 gene orf cdna clone expression plasmid, c-gfpspark tag
Rat Mlc1 Gene Orf Cdna Clone Expression Plasmid, C Gfpspark Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/Rat+MLC1+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-GFPSpark+tag/sino+biological___rg86578
Average 93 stars, based on 1 article reviews
rat mlc1 gene orf cdna clone expression plasmid, c-gfpspark tag - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

86
Accurate Biotechnology Co Ltd plasmid mini kit
Plasmid Mini Kit, supplied by Accurate Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/ag21001+kit+mini+plasmid/pmc13197805-106-10-15
Average 86 stars, based on 1 article reviews
plasmid mini kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Sangon Biotech plasmids
Plasmids, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/plasmids/pmc12993211-57-2-9
Average 86 stars, based on 1 article reviews
plasmids - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Genechem firefly luciferase reporter plasmids
Firefly Luciferase Reporter Plasmids, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/abcb11+construct+gv238+luciferase+promoter+reporter/pmc13254595-132-12-23
Average 86 stars, based on 1 article reviews
firefly luciferase reporter plasmids - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

93
Addgene inc addgene 43802
Addgene 43802, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-10-10
Average 93 stars, based on 1 article reviews
addgene 43802 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

86
Sangon Biotech sanprep column plasmid mini preps kit
Sanprep Column Plasmid Mini Preps Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/column+dna+extraction+gel+kit+sanprep/pmc12907642-86-9-25
Average 86 stars, based on 1 article reviews
sanprep column plasmid mini preps kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Yeasen Biotechnology universal enzyme construct plasmid
Universal Enzyme Construct Plasmid, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/construct+enzyme+plasmid+universal/pmc12905773-55-16-9
Average 86 stars, based on 1 article reviews
universal enzyme construct plasmid - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

93
Addgene inc p414
P414, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-0-10
Average 93 stars, based on 1 article reviews
p414 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc tef1p cas9 cyc1t
Tef1p Cas9 Cyc1t, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-4-10
Average 93 stars, based on 1 article reviews
tef1p cas9 cyc1t - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results