p414 (Addgene inc)
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p414
P414, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-0-10
Average 93 stars, based on 128 article reviews
P414, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-0-10
Average 93 stars, based on 128 article reviews
p414 - by Bioz Stars,
2026-11
93/100 stars
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Clone Assay:Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the Cloning:Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the Plasmid Preparation:Article Title: Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis Article Snippet: Primers, plasmids, strains, genes used in this study are summarized in p414-TEF1p-Cas9-CYC1t (Addgene 43802) was obtained from Addgene. .. The FnCas12a gene from Francisella novicida was codon-optimized, cloned into the Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system Article Snippet: .. The operations are as follows: The codon-optimized Cas9 from Streptococcus pyogenes was obtained from the Article Title: ROS signaling mediates aeration-dependent thermotolerance in Kluyveromyces marxianus for high-temperature xylitol production. Article Snippet: This study uncovers that moderate reactive oxygen species (ROS) signaling mediates aeration-dependent thermotolerance in Kluyveromyces marxianus, challenging the long-held paradigm that ROS function solely as toxic metabolic byproducts.. Through integrated transcriptomic profiling and RT-qPCR validation, 19 key transcription factors regulating this adaptive response were identified, and functional assays demonstrated that targeted knockout of GSF2 and RIM101 coupled with MED15 overexpression significantly enhances hypoxic thermotolerance.. The ERG6-overexpressing strain YZB559 yields 72.45 g/L xylitol at 45 ◦C under medium–high oxygen (MHO) condition, marking a 20% improvement over the parental strain, while the combinatorially engineered YZB639 (ΔGSF2::MED15) achieves 74.13 g/L xylitol with complete xylose consumption at 46 ◦C and 30.64 g/L ethanol under micro-oxygen conditions, representing a 23% increase in ethanol production. Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a Expressing:Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system Article Snippet: .. The operations are as follows: The codon-optimized Cas9 from Streptococcus pyogenes was obtained from the other:Article Title: Engineering Kluyveromyces marxianus for 3-hydroxypropionic acid production at elevated temperature from Jerusalem artichoke tubers and crude glycerol Article Snippet: p414 , CRISPR:Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a Selection:Article Title: Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing Article Snippet: .. CRISPR-Cas9 endonuclease experiment was performed similarly as described by DiCarlo et al. ( ): SpCas9 endonuclease was firstly expressed from a |