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plasmid mini prep kit  (Zymo Research)


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    Structured Review

    Zymo Research plasmid mini prep kit
    Plasmid Mini Prep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 98/100, based on 374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+mini+prep+kit/ZymoPURE+Plasmid+Miniprep+Kit/pmc13106971-397-9-7
    Average 98 stars, based on 374 article reviews
    plasmid mini prep kit - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Expressing:

    Article Title: Albumin-Coated Gold Nanoparticles Functionalised with a Gadolinium-Binding Recombinant Protein for Dual CT/MR Imaging
    Article Snippet: Desalting used Zeba Spin Desalting Columns, 7 K MWCO (89882, Thermo Fisher Scientific) or PD-10 columns packed with Sephadex G-25 M (GE17085101, Cytiva, Marlborough, MA, USA). .. Reagents, enzymes, kits and strains used for the genetic construction and expression of FAG (pRSET-EmGFP vector V35320, Thermo Fisher Scientific; BamHI-HF, XhoI, T4 DNA ligase, Q5 High-Fidelity DNA Polymerase and Escherichia coli NiCo21(DE3), New England Biolabs, Ipswich, MA, USA; ZymoPURE Plasmid Miniprep Kit, Zymo Research, Irvine, CA, USA; HisPur Ni-NTA resin, Thermo Fisher Scientific; oligonucleotides, Syntol LLC, Moscow, Russia) are listed together with the construction procedure in Supplementary Materials (File S1). ..

    Plasmid Preparation:

    Article Title: Albumin-Coated Gold Nanoparticles Functionalised with a Gadolinium-Binding Recombinant Protein for Dual CT/MR Imaging
    Article Snippet: Desalting used Zeba Spin Desalting Columns, 7 K MWCO (89882, Thermo Fisher Scientific) or PD-10 columns packed with Sephadex G-25 M (GE17085101, Cytiva, Marlborough, MA, USA). .. Reagents, enzymes, kits and strains used for the genetic construction and expression of FAG (pRSET-EmGFP vector V35320, Thermo Fisher Scientific; BamHI-HF, XhoI, T4 DNA ligase, Q5 High-Fidelity DNA Polymerase and Escherichia coli NiCo21(DE3), New England Biolabs, Ipswich, MA, USA; ZymoPURE Plasmid Miniprep Kit, Zymo Research, Irvine, CA, USA; HisPur Ni-NTA resin, Thermo Fisher Scientific; oligonucleotides, Syntol LLC, Moscow, Russia) are listed together with the construction procedure in Supplementary Materials (File S1). ..

    Article Title: Targeting Cryptic Splicing Variants in SDCCAG8, CEP290, and ABCA4: A Translational Workflow from Diagnosis to ASO-Based Therapy
    Article Snippet: The PCR products were then cloned 428 into a pGEM-T Easy Vector (Promega) and transformed into JM109 competent E. coli cells, following 429 the manufacturer’s protocol. .. Plasmids containing either the wild-type (from the control; TA-ABCA4-430 WT) or the mutated sequence (from the proband; TA-ABCA4-MUT) were extracted using 431 ZymoPURE Plasmid MiniPrep (Zymo Research) and subsequently subcloned into the pSPL3 vector 432 (Life Sciences-Invitrogen). .. The final pSPL3 plasmids (+ABCA4-WT or -MUT) were transfected into 433 HeLa cells using Lipofectamine 2000 (Thermo Fisher Scientific).

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: .. Plasmid DNA was extracted from grown cultures and purified using a ZymoPURE Plasmid Miniprep kit (Zymo Research, D4210) following the manufacturer’s instructions. .. Purified DNA was quantified using a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific).

    Article Title: Production of antimicrobial peptides fused to a nanoparticle-forming polypeptide using Pichia pastoris
    Article Snippet: Taq Master Mix (2 ×), SimplyBlue SafeStain, Live/Dead BacLight bacterial viability kit, and Hoechst 33342 were obtained from Thermo Scientific (Waltham, MA, USA). .. Plasmid extractions were performed using the ZymoPURE Plasmid Miniprep Kit from Zymo Research (Irvine, CA, USA). .. Primers were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Article Title: Biological Characterisation of a Divergent Dragon Fruit ( Hylocereus spp.) Strain of Cactus Carlavirus‐2 ( CCV ‐2_Pit)
    Article Snippet: .. Overnight cultures of transformed E. coli, previously selected by colony PCR, were subjected to plasmid extraction using the ZymoPURE Plasmid MiniPrep Kit (ZymoResearch). ..

    Article Title: Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis
    Article Snippet: .. Plasmid DNA was extracted using the ZymoPURE Plasmid Miniprep Kit (Zymo Research), and the insert was sequence‐confirmed via Sanger sequencing. ..

    Article Title: Epigenetic activation of EBV BGLF4 determines antiviral-based regimen response in EBV+CNS lymphoproliferative disease
    Article Snippet: DNA and RNA from cell culture were isolated using the QIAamp DNA Mini Kit (QIAGEN) and TRIzol reagent (Invitrogen), respectively. .. Plasmid and lentiviral vector DNA were prepared using the ZymoPURE Plasmid Miniprep Kit (Zymo Research) and the PureYield Plasmid Midiprep System (Promega). ..

    Article Title: RNA polymerase I inhibitor BMH-21 promotes mutant p53 reactivation through nuclear zinc mobilization.
    Article Snippet: BMH-21, APR-246, and ZMC1 were dissolved in DMSO (Sigma-Aldrich). .. Plasmid transfections were performed with endotoxin-free plasmid preparations produced with the ZymoPURE Plasmid Miniprep Kit (Zymo Research). .. Cells were transfected using Lipofectamine 3000 (ThermoFisher) per manufacturer’s instructions.

    Control:

    Article Title: Targeting Cryptic Splicing Variants in SDCCAG8, CEP290, and ABCA4: A Translational Workflow from Diagnosis to ASO-Based Therapy
    Article Snippet: The PCR products were then cloned 428 into a pGEM-T Easy Vector (Promega) and transformed into JM109 competent E. coli cells, following 429 the manufacturer’s protocol. .. Plasmids containing either the wild-type (from the control; TA-ABCA4-430 WT) or the mutated sequence (from the proband; TA-ABCA4-MUT) were extracted using 431 ZymoPURE Plasmid MiniPrep (Zymo Research) and subsequently subcloned into the pSPL3 vector 432 (Life Sciences-Invitrogen). .. The final pSPL3 plasmids (+ABCA4-WT or -MUT) were transfected into 433 HeLa cells using Lipofectamine 2000 (Thermo Fisher Scientific).

    Sequencing:

    Article Title: Targeting Cryptic Splicing Variants in SDCCAG8, CEP290, and ABCA4: A Translational Workflow from Diagnosis to ASO-Based Therapy
    Article Snippet: The PCR products were then cloned 428 into a pGEM-T Easy Vector (Promega) and transformed into JM109 competent E. coli cells, following 429 the manufacturer’s protocol. .. Plasmids containing either the wild-type (from the control; TA-ABCA4-430 WT) or the mutated sequence (from the proband; TA-ABCA4-MUT) were extracted using 431 ZymoPURE Plasmid MiniPrep (Zymo Research) and subsequently subcloned into the pSPL3 vector 432 (Life Sciences-Invitrogen). .. The final pSPL3 plasmids (+ABCA4-WT or -MUT) were transfected into 433 HeLa cells using Lipofectamine 2000 (Thermo Fisher Scientific).

    Article Title: Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis
    Article Snippet: .. Plasmid DNA was extracted using the ZymoPURE Plasmid Miniprep Kit (Zymo Research), and the insert was sequence‐confirmed via Sanger sequencing. ..

    Purification:

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: .. Plasmid DNA was extracted from grown cultures and purified using a ZymoPURE Plasmid Miniprep kit (Zymo Research, D4210) following the manufacturer’s instructions. .. Purified DNA was quantified using a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific).

    Transformation Assay:

    Article Title: Biological Characterisation of a Divergent Dragon Fruit ( Hylocereus spp.) Strain of Cactus Carlavirus‐2 ( CCV ‐2_Pit)
    Article Snippet: .. Overnight cultures of transformed E. coli, previously selected by colony PCR, were subjected to plasmid extraction using the ZymoPURE Plasmid MiniPrep Kit (ZymoResearch). ..

    Polymerase Chain Reaction:

    Article Title: Biological Characterisation of a Divergent Dragon Fruit ( Hylocereus spp.) Strain of Cactus Carlavirus‐2 ( CCV ‐2_Pit)
    Article Snippet: .. Overnight cultures of transformed E. coli, previously selected by colony PCR, were subjected to plasmid extraction using the ZymoPURE Plasmid MiniPrep Kit (ZymoResearch). ..

    Extraction:

    Article Title: Biological Characterisation of a Divergent Dragon Fruit ( Hylocereus spp.) Strain of Cactus Carlavirus‐2 ( CCV ‐2_Pit)
    Article Snippet: .. Overnight cultures of transformed E. coli, previously selected by colony PCR, were subjected to plasmid extraction using the ZymoPURE Plasmid MiniPrep Kit (ZymoResearch). ..

    DNA Sequencing:

    Article Title: Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis
    Article Snippet: .. Plasmid DNA was extracted using the ZymoPURE Plasmid Miniprep Kit (Zymo Research), and the insert was sequence‐confirmed via Sanger sequencing. ..

    Transfection:

    Article Title: RNA polymerase I inhibitor BMH-21 promotes mutant p53 reactivation through nuclear zinc mobilization.
    Article Snippet: BMH-21, APR-246, and ZMC1 were dissolved in DMSO (Sigma-Aldrich). .. Plasmid transfections were performed with endotoxin-free plasmid preparations produced with the ZymoPURE Plasmid Miniprep Kit (Zymo Research). .. Cells were transfected using Lipofectamine 3000 (ThermoFisher) per manufacturer’s instructions.

    Produced:

    Article Title: RNA polymerase I inhibitor BMH-21 promotes mutant p53 reactivation through nuclear zinc mobilization.
    Article Snippet: BMH-21, APR-246, and ZMC1 were dissolved in DMSO (Sigma-Aldrich). .. Plasmid transfections were performed with endotoxin-free plasmid preparations produced with the ZymoPURE Plasmid Miniprep Kit (Zymo Research). .. Cells were transfected using Lipofectamine 3000 (ThermoFisher) per manufacturer’s instructions.



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