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physiologic buffer solution (Thermo Fisher)

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Thermo Fisher physiologic buffer solution
Physiologic Buffer Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/physiologic+buffer+solution/Buffer+solution+pH+7%2C+Phosphate+buffer/pmc13080204-38-34-51
Average 97 stars, based on 1 article reviews

physiologic buffer solution - by Bioz Stars, 2026-10

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Article Title: Optogenetic cross-linking of the actin cytoskeleton using DARPins
Article Snippet: .. DARPins were separated from unreacted peptide and the buffer was replaced with phosphate buffer (48 mM K2HPO4, 4.5 mM KH2PO4, 14 mM NaH2PO4 in distilled water at pH 7.2) by ZebaSpin Desalting columns with a molecular weight cut-off at 7 kDa (Thermo Fisher, #78606). ..

Spectrophotometry:

Article Title: Ultrasound-driven ternary conjugation reshapes protein-lipid interfaces and volatile profiles in high-protein egg milk systems.
Article Snippet: .. Samples were diluted to 0.2 mg/ mL in 10 mM phosphate buffer (pH 7.0) and scanned from 240 nm to 340 nm at 25 ◦C using a UV spectrophotometer (Thermo Fisher Scientific, MA, USA) with a 1 cm quartz cuvette. ..

Quartz:

Negative Control:

Article Title: Fc region variant
Article Snippet: .. A sample prepared by adding a phosphate buffer (pH 7.4) (Gibco) instead of the immune complex was used as the negative control. .. The produced plasmids were introduced transiently into the HEK293H cell line derived from human embryonic kidney cancer cells (Invitrogen) or into FreeStyle293 cells (Invitrogen) for antibody expression.

Membrane:

Article Title: Human 3D epithelioids enable continuous long-term clonal evolution studies across multiple epithelial tissues
Article Snippet: .. Membrane biopsies from human epithelioid cultures were obtained by using a 5 mm diameter biopsy punch, and fixed in a fixative solution containing 4% PFA (Sigma-Aldrich, P6148-500G) and 2.5% glutaraldehyde (EM grade distillation purified, Electron Microscopy Sciences, 16210) in 0.1 M phosphate buffer pH 7.4 (PB) (0.2 M Na 2 HPO 4 , Acros Organics, 448150010; 54 mM NaH 2 PO 4 , Acros Organics, 447760010; dissolved in ddH 2 O, pH 7.4) at 37°C for 15 minutes. .. Membrane biopsies from human epithelioid cultures were obtained by using a 5 mm diameter biopsy punch, and fixed in a fixative solution containing 4% PFA (Sigma-Aldrich, P6148-500G) and 2.5% glutaraldehyde (EM grade distillation purified, Electron Microscopy Sciences, 16210) in 0.1 M phosphate buffer pH 7.4 (PB) (0.2 M Na 2 HPO 4 , Acros Organics, 448150010; 54 mM NaH 2 PO 4 , Acros Organics, 447760010; dissolved in ddH 2 O, pH 7.4) at 37°C for 15 minutes.

Distillation:

Purification:

Electron Microscopy:

Article Title: Heparin-like compounds improve the biophysical properties of eosinophilic mucus.
Article Snippet: .. EETosis cells treated with or without heparin (300 μg/mL, 30 min) were fixed (1% glutaraldehyde and 1% paraformaldehyde) in 0.1 M phosphate buffer (pH 7.4) for 1 h at room temperature and analyzed by scanning electron microscopy (FEI Quanta 3D FEG Dual Beam). ..

Muscles:

Article Title: Genetic Rescue of a Lethal Wasting Mutation ( Syb1 lew/lew ) by Neuron-Specific Expression of ECFP-Syb2
Article Snippet: .. Briefly, lumbrical or triangularis sterni muscles were fixed in 2% paraformaldehyde in 0.1 M phosphate buffer (pH 7.3) overnight at 4 ° C. Muscle samples were then incubated with Texas-Red conjugated α-bungarotoxin (α-bgt) (2 nM, Invitrogen, Carlsbad, California, USA) for 30 minutes, followed by incubation with primary antibodies diluted in antibody dilution buffer (500 mM NaCl, 0.01 M phosphate buffer, 3% BSA and 0.01% thimerosal) overnight at 4 ° C. The following primary antibodies were used: rabbit polyclonal anti-synaptotagmin2 (I735) , rabbit polyclonal anti-synaptobrevin1 (P938) and rabbit polyclonal anti-synaptobrevin2 (P939) (generous gifts from Dr. Thomas Südhof, Stanford University School of Medicine, Palo Alto, CA, USA). .. After extensive washes, muscle samples were incubated overnight at 4 ° C with Alexa Fluor 647-conjugated goat anti-rabbit IgG (1:600, Jackson ImmunoResearch Laboratories).

Article Title: Loss of ACTA1 leads to delayed γ-AChR / ε-AChR switch in skeletal muscle in mice
Article Snippet: .. Briefly, either diaphragm or triangularis sterni muscles from Acta1 −/− and their littermate controls ( Acta1 +/+ or Acta1 +/− ) at E18.5 or P5 were fixed in 2% paraformaldehyde in 0.1 M phosphate buffer (pH 7.3) overnight at 4 ° C. Samples were extensively washed with PBS and then incubated with Texas-Red conjugated α-bungarotoxin (α-bgt) (2 nM, Invitrogen, Carlsbad, California, USA) for 30 minutes at room temperature. .. Samples were then incubated with primary antibodies overnight at 4 ° C. The following polyclonal antibodies were used: neurofilament (NF150) (Chemicon, Temecula, CA, USA), synaptotagmin 2 (I735), syntaxin 1(I375) (generous gifts from Dr. Thomas Südhof, Stanford University School of Medicine, Palo Alto, CA, USA) , and acetylcholinesterase (AChE) (generous gifts from Dr. Palmer Taylor, UC San Diego, CA, USA).

Incubation:

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$Experimental set-up for irradiation and OCT-imaging of the murine Arteria saphena. A For irradiation in the X-ray device (1), anesthetized animals were positioned on their left side and secured on a Plexiglas holder. The bent right leg and lower abdomen were shielded with lead to protect them from radiation, ensuring that only the inner side of the left lower leg remained within the irradiation field. The exposure area beneath the irradiation window was defined by a collimator plate made of a bismuth-lead-tin alloy (MCP-96) with copper cutouts (2). Up to five animals were irradiated simultaneously on an underlying Plexiglas plate (3). The mesures are given in centimeters. B The OCT system for vascular imaging of the A. saphena operates using near-infrared light emitted by a diode (1), which is transmitted to the scanner head (2) via fiber optic cables (3). Within the scanner head the incoming light is collimated to a beam of 2.4 mm in diameter through a collimator (4) (focal length = 12 mm) and subsequently divided into a reference and probe beam of equal diameter with a beam splitter. To scan the arterial surface, the probe beam is diffracted via two galvanometric scanners (5) (Cambridge Technologies, Planegg) and focused through an achromatic lense (6) (focal length = 25.4 mm, diameter = 15 mm). The light reflected by the arterial surface and the reference beam that has been reflected by a mirror are then recombined by the beam splitter. Fiber optic cables lead the resulting interference signal through a collimator (focal length = 40 mm) and to a spectrometer to be spectrally analyzed with a diffraction grating (1200 lines/mm). The interference spectrum is then focused through an achromatic lense (focal length = 75 mm) and detected with a silicon detector (LIS-1024, pixel size: 7.8 μm × 125 μm × 1024 px, Photon Vision Systems Inc., Homer, USA). A Fast Fourier Transform of the interference signal provides depth-resolved information about the arterial tissue. C Representative recording of the A. saphena (white arrows) and Vena saphena medialis (grey arrows) of a C57BL/6 mouse aged 8 weeks, one day after irradiation with 2 Gy. The upper picture row in the foreground represents 2-D cross sectional OCT-images. The picture row below in the background are video-recordings to orientate on the tissue. Left: Vessel diameter at rest after application of physiological buffer solution. Middle: Arterial vasoconstriction (VC) after application of buffer solution with high potassium concentration (K+). Right: Arterial vasodilation (VD) induced by sodium nitroprusside (SNP). The diameter of the saphenous vein was unaffected. Below: Time course of inner diameter changes of A. saphena with fitted sigmoid function (black line). d0: initial diameter, dVC: minimal diameter during VC. dVD: maximal diameter during VD. t 1/2 : time of half VC or VD$

Journal: Cardio-oncology

Article Title: Radiation- and age-related vascular dysfunction as an early indicator of cardiovascular risk: a long-term study in the ApoE −/− mouse model of atherosclerosis

doi: 10.1186/s40959-025-00395-6

Figure Lengend Snippet: Experimental set-up for irradiation and OCT-imaging of the murine Arteria saphena. A For irradiation in the X-ray device (1), anesthetized animals were positioned on their left side and secured on a Plexiglas holder. The bent right leg and lower abdomen were shielded with lead to protect them from radiation, ensuring that only the inner side of the left lower leg remained within the irradiation field. The exposure area beneath the irradiation window was defined by a collimator plate made of a bismuth-lead-tin alloy (MCP-96) with copper cutouts (2). Up to five animals were irradiated simultaneously on an underlying Plexiglas plate (3). The mesures are given in centimeters. B The OCT system for vascular imaging of the A. saphena operates using near-infrared light emitted by a diode (1), which is transmitted to the scanner head (2) via fiber optic cables (3). Within the scanner head the incoming light is collimated to a beam of 2.4 mm in diameter through a collimator (4) (focal length = 12 mm) and subsequently divided into a reference and probe beam of equal diameter with a beam splitter. To scan the arterial surface, the probe beam is diffracted via two galvanometric scanners (5) (Cambridge Technologies, Planegg) and focused through an achromatic lense (6) (focal length = 25.4 mm, diameter = 15 mm). The light reflected by the arterial surface and the reference beam that has been reflected by a mirror are then recombined by the beam splitter. Fiber optic cables lead the resulting interference signal through a collimator (focal length = 40 mm) and to a spectrometer to be spectrally analyzed with a diffraction grating (1200 lines/mm). The interference spectrum is then focused through an achromatic lense (focal length = 75 mm) and detected with a silicon detector (LIS-1024, pixel size: 7.8 μm × 125 μm × 1024 px, Photon Vision Systems Inc., Homer, USA). A Fast Fourier Transform of the interference signal provides depth-resolved information about the arterial tissue. C Representative recording of the A. saphena (white arrows) and Vena saphena medialis (grey arrows) of a C57BL/6 mouse aged 8 weeks, one day after irradiation with 2 Gy. The upper picture row in the foreground represents 2-D cross sectional OCT-images. The picture row below in the background are video-recordings to orientate on the tissue. Left: Vessel diameter at rest after application of physiological buffer solution. Middle: Arterial vasoconstriction (VC) after application of buffer solution with high potassium concentration (K+). Right: Arterial vasodilation (VD) induced by sodium nitroprusside (SNP). The diameter of the saphenous vein was unaffected. Below: Time course of inner diameter changes of A. saphena with fitted sigmoid function (black line). d0: initial diameter, dVC: minimal diameter during VC. dVD: maximal diameter during VD. t 1/2 : time of half VC or VD

Article Snippet: To assess the arterial diameter at baseline the exposed A. saphena was moistened with a physiological buffer solution (NaCl: 119 mmol/l, Merck, Darmstadt, Germany; KCl: 4.7 mmol/l, Merck; MgSO 4 : 1.17 mmol/l, Sigma-Aldrich, Taufkirchen, Germany; NaHCO 3 : 25 mmol/l, Merck; KH 2 PO 4 : 1.18 mmol/l, Merck; Glucose: 5.5 mmol/l, Merck; EDTA: 0.027 mmol/l, Prolabo, VWR International, Darmstadt) right before starting OCT. Acquisition of the baseline diameter stopped automatically after 30 initial B-scans (equivalent to a recording time of 7.5 s).

Techniques: Irradiation, Imaging, Concentration Assay