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Sangon Biotech phosphate buffer saline pbs solution
Phosphate Buffer Saline Pbs Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphate+buffer+solution/buffered+phosphate+saline/pm42257551-73-7-15
Average 86 stars, based on 1 article reviews
phosphate buffer saline pbs solution - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Transfection:

Article Title: Dual Pathways of UBE4B Inhibit Apoptosis in p53-Positive Tumor Cells via CCAR2 Degradation.
Article Snippet: Cell apoptosis was assessed by flow cytometry using an Annexin V-FITC/PI apoptosis detection kit (Biosharp, Beijing, China, BL107A). .. Cells were transfected with siRNAs for 72 h. After transfection, the cells were harvested by trypsinization (without EDTA) and washed twice with phosphate-buffered saline (PBS, Sangon Biotech, B640011-0010) via centrifugation at 800× g for 3 min per wash. For staining, approximately 5 × 105 cells were resuspended in 500 μL of PBS. ..

Article Title: Dual-Site Cooperative Recognition Hybridization Chain Reaction for High-Sensitivity Imaging of lncRNA.
Article Snippet: Developing highly sensitive and specific imaging strategies for long noncoding RNAs (lncRNAs) is crucial for disease diagnosis and therapeutic research.. However, lncRNAs are characterized by long sequence lengths (>200 nt), low abundance, and complex secondary structures, which make their sensitive and specific imaging challenging.. To address these challenges, we developed a hybridization chain reaction (HCR) strategy based on dual-site cooperative recognition for lncRNA imaging, termed dualsite cooperative recognition HCR (DCHCR).

Saline:

Article Title: Dual Pathways of UBE4B Inhibit Apoptosis in p53-Positive Tumor Cells via CCAR2 Degradation.
Article Snippet: Cell apoptosis was assessed by flow cytometry using an Annexin V-FITC/PI apoptosis detection kit (Biosharp, Beijing, China, BL107A). .. Cells were transfected with siRNAs for 72 h. After transfection, the cells were harvested by trypsinization (without EDTA) and washed twice with phosphate-buffered saline (PBS, Sangon Biotech, B640011-0010) via centrifugation at 800× g for 3 min per wash. For staining, approximately 5 × 105 cells were resuspended in 500 μL of PBS. ..

Article Title: Osteoblast‐Derived Osteomodulin Suppresses Bone Loss in Osteoporosis by Inhibiting Osteoclastogenesis
Article Snippet: .. Cells were washed twice with 1× phosphate- buffered saline (PBS) and fixed with 4% paraformaldehyde (PFA; Sangon Biotech, Shanghai, China) for 20 min. After washing, cells were stained with 0.1% ARS (Beyotime, Shanghai, China) solution for 30 min. ALP staining was carried out using an ALP Staining Kit (Beyotime) according to the manufacturer's instructions. ..

Article Title: Yupingfeng San extract ameliorates cisplatin induced renal interstitial fibrosis via IL-6/JAK/STAT3 mediated restoration of mitochondrial homeostasis.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: Deep eutectic solvent tailoring the electrode-enzyme interface for phenylalanine dehydrogenase immobilization and efficient bioelectrosynthesis.
Article Snippet: .. Readyto-use phosphate buffered saline (PBS) powder was purchased from Shanghai Sangon Biotech Co., Ltd. (Shanghai, China). ..

Article Title: Hypoxia-preconditioned adipose-derived mesenchymal stem cells-derived exosomes transferring H19 obstruct neutrophil extracellular traps formation via HOXA5-mediated inactivation of TLR4/NF-κB/NLRP3 inflammatory signaling.
Article Snippet: Human neutrophil isolation was performed using a Human peripheral blood neutrophil isolation solution kit (#P9040, Solarbio Biotech, Beijing, China), following the manufacturer’s protocol. .. Briefly, human peripheral blood was supplemented with phosphate buffer saline (PBS) solution (#C500623, 1×, 30%, Sangon Biotech, Shanghai, China) and erythrocyte sedimentation solution (30%, #P9040, Solarbio Biotech). ..

Article Title: Modular CRISPR-Cas12a-Activated Gold Nanoparticle Assay for Rapid Visual Detection of Hepatocellular Carcinoma-Related miRNAs.
Article Snippet: ). he ABSTRACT: MicroRNAs (miRNAs) are emerging biomarkers for early hepatocellular carcinoma (HCC) detection, but most CRISPR−Cas12a assays rely on reverse transcription and preamplification and often lack simple visual readouts.. Here, we develop a reverse transcription-free, cleavage-guided strategy that converts miRNA recognition into visual outputs.. In the presence of the target miRNA, a scaffold RNA forms an active crRNA that triggers Cas12a trans-cleavage using a preformed dsDNA activator, enabling two readout formats.

Article Title: Flexible regulation of CRISPR/Cas12a activity by spatial confinement effect
Article Snippet: .. HPLC-purified oligonucleotides , TE buffer, RNase inhibitor, 10 × phosphate-buffered saline (PBS), RNase-free water, and all DNase/RNase-free tips were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). .. 6 × loading buffer and 20 bp DNA Marker were obtained from Takara Biotech Inc. (Dalian, China).

Centrifugation:

Article Title: Dual Pathways of UBE4B Inhibit Apoptosis in p53-Positive Tumor Cells via CCAR2 Degradation.
Article Snippet: Cell apoptosis was assessed by flow cytometry using an Annexin V-FITC/PI apoptosis detection kit (Biosharp, Beijing, China, BL107A). .. Cells were transfected with siRNAs for 72 h. After transfection, the cells were harvested by trypsinization (without EDTA) and washed twice with phosphate-buffered saline (PBS, Sangon Biotech, B640011-0010) via centrifugation at 800× g for 3 min per wash. For staining, approximately 5 × 105 cells were resuspended in 500 μL of PBS. ..

Staining:

Article Title: Dual Pathways of UBE4B Inhibit Apoptosis in p53-Positive Tumor Cells via CCAR2 Degradation.
Article Snippet: Cell apoptosis was assessed by flow cytometry using an Annexin V-FITC/PI apoptosis detection kit (Biosharp, Beijing, China, BL107A). .. Cells were transfected with siRNAs for 72 h. After transfection, the cells were harvested by trypsinization (without EDTA) and washed twice with phosphate-buffered saline (PBS, Sangon Biotech, B640011-0010) via centrifugation at 800× g for 3 min per wash. For staining, approximately 5 × 105 cells were resuspended in 500 μL of PBS. ..

Article Title: Osteoblast‐Derived Osteomodulin Suppresses Bone Loss in Osteoporosis by Inhibiting Osteoclastogenesis
Article Snippet: .. Cells were washed twice with 1× phosphate- buffered saline (PBS) and fixed with 4% paraformaldehyde (PFA; Sangon Biotech, Shanghai, China) for 20 min. After washing, cells were stained with 0.1% ARS (Beyotime, Shanghai, China) solution for 30 min. ALP staining was carried out using an ALP Staining Kit (Beyotime) according to the manufacturer's instructions. ..

ALP Assay:

Article Title: Osteoblast‐Derived Osteomodulin Suppresses Bone Loss in Osteoporosis by Inhibiting Osteoclastogenesis
Article Snippet: .. Cells were washed twice with 1× phosphate- buffered saline (PBS) and fixed with 4% paraformaldehyde (PFA; Sangon Biotech, Shanghai, China) for 20 min. After washing, cells were stained with 0.1% ARS (Beyotime, Shanghai, China) solution for 30 min. ALP staining was carried out using an ALP Staining Kit (Beyotime) according to the manufacturer's instructions. ..

Sedimentation:

Article Title: Hypoxia-preconditioned adipose-derived mesenchymal stem cells-derived exosomes transferring H19 obstruct neutrophil extracellular traps formation via HOXA5-mediated inactivation of TLR4/NF-κB/NLRP3 inflammatory signaling.
Article Snippet: Human neutrophil isolation was performed using a Human peripheral blood neutrophil isolation solution kit (#P9040, Solarbio Biotech, Beijing, China), following the manufacturer’s protocol. .. Briefly, human peripheral blood was supplemented with phosphate buffer saline (PBS) solution (#C500623, 1×, 30%, Sangon Biotech, Shanghai, China) and erythrocyte sedimentation solution (30%, #P9040, Solarbio Biotech). ..

High Performance Liquid Chromatography:

Article Title: Flexible regulation of CRISPR/Cas12a activity by spatial confinement effect
Article Snippet: .. HPLC-purified oligonucleotides , TE buffer, RNase inhibitor, 10 × phosphate-buffered saline (PBS), RNase-free water, and all DNase/RNase-free tips were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). .. 6 × loading buffer and 20 bp DNA Marker were obtained from Takara Biotech Inc. (Dalian, China).



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