other:Article Title: Combined Menin and XPO1 inhibition drive synergistic antileukemic activity in KMT2A r and NPM1 -m AML
Article Snippet: The protein samples were subjected to SDS-PAGE using precast gel (Bio-Rad, Hercules, CA, USA) and then transferred onto nitrocellulose (Amersham, Germany; catalog no. 10600006) or polyvinylidene fluoride (PVDF) membranes (Sigma, IPVH00010) by electrophoresis.
Recombinant:Article Title: Humidity shapes reproductive development in Arabidopsis thaliana by modulating epidermal identity and cell wall dynamics.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Technovit 7100 combipack Heraeus Kulzer Cat# 64709003 Nitroblue tetrazolium Sigma-Aldrich Cat#N6639 3,3-diaminobenzidine Sigma-Aldrich Cat#D5637 Toluidine blue O ThermoFisher Scientific Cat#BP10710 Piperonylic acid Sigma-Aldrich Cat#P49805 Coniferyl alcohol Sigma-Aldrich Cat#223735 Sinapyl alcohol Sigma-Aldrich Cat#404586 Phloroglucinol Sigma-Aldrich Cat#P3502 Epigallocatechin gallate Sigma-Aldrich Cat#44901 Pectinase from Aspergillus aculeatus Sigma-Aldrich Cat#P2611 Aminoethoxyvinylglycine Cayman Chemical Cat#15546 6-benzylaminopurine KisanBio Cat#B5812 β-estradiol Sigma-Aldrich Cat#43632 Paraformaldehyde Sigma-Aldrich Cat#A11313 Phosphate buffered saline Bio-Rad Cat1610780 ClearSee Ursache et al. 60 N/A Calcofluor whte Sigma-Aldrich Cat#18909 Basic fuchsin Sigma-Aldrich Cat#B0904 Propidium iodide Sigma-Aldrich Cat#537059 Fluorescein diacetate Sigma-Aldrich Cat#F7378 Tween 20 Sigma-Aldrich Cat#P1379 Lip balm Nivea Cat#88070 4-Head Industrial Commercial Electric Ultrasonic Atomizer Mist Maker Humidifier Fogger Foshan Nanhai Keri Electronic Co., Ltd. KERI M0403 Digital thermo-hygrometer SATO SK-110TRHII TYPE 4 Isopropanol Sigma-Aldrich Cat#278475 Hexane Sigma-Aldrich Cat#52750 Chloroform Sigma-Aldrich Cat#319988 Heptadecanoate Sigma-Aldrich Cat#286087 ω-pentadecalactone Sigma-Aldrich Cat#W284009 N,O-bis(trimethylsilyl) trifluoroacetamide Sigma-Aldrich Cat#155195 Pyridine Sigma-Aldrich Cat#360570 Heptane Sigma-Aldrich Cat#34873 Toluene solution Sigma-Aldrich Cat#CRM48620 Aniline Blue diammonium salt Sigma-Aldrich Cat#415049 amfiRivert cDNA Synthesis Mix GenDEPOT Cat#R5600-050 SYBR Green qPCR Master Mix ThermoFisher Scientific Cat#A25742 Critical commercial assays RNeasy Plant Mini Kit QIAGEN Cat#74094 TruSeq Stranded mRNA Library Prep kit Illumina Cat#20020611 Illumina HiSeq 2500 platform Illumina Cat#SY-401-2501 (Continued on next page) 18 Cell Reports 45, 116707, January 27, 2026 ..
Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Monoclonal EZH1 antibody, 1:1000 dilution Cell Signaling Technology Cat# 42088; RRID: AB_2799212 Rabbit Monoclonal KMT6/EZH2 antibody, 1:1000 dilution Abcam Cat# ab191250; RRID: AB_2933983 Rabbit Monoclonal H3K27me3 antibody, 1:1000 dilution ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749. .. Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026
Article Title: An integrative spatial multi-omic workflow for unified analysis of tumor tissue.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Alexa Fluor® 647 phalloidin Thermo Fisher Scientific A22287 GFAP Thermo Fisher Scientific Cat# PA1-10004; RRID: AB_1074620 DAPI Thermo Fisher Scientific Cat# D1306; RRID: AB_2629482 Alexa Fluor 488 Plus Thermo Fisher Scientific Cat# A11039; RRID: AB_2534096 Nuclease-free Water (not DEPC treated) Thermo Fisher Scientific AM9937 TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0 (nuclease-free) Fisher Scientific BP24731 PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free Thermo Fisher Scientific AM9624 10% Tween 20 Bio-Rad 1662404 Ethyl alcohol, Pure (200 Proof, anhydrous) Millipore Sigma E7023-500ML Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 Methanol Methanol, for HPLC Millipore Sigma 34860 Critical commercial assays Xenium Slides & Sample Prep Reagents 10× Genomics PN-1000460 Xenium Decoding Consumables 10× Genomics PN-1000487 Xenium Instrument Accessory Kit, Module A 10× Genomics PN-1000530 Xenium Stand Alone Gene Expression Panel 10× Genomics PN-1000563 ZQ94XW Deposited data Analyzed ST data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Analyzed SM data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Processed tile scans This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Software and algorithms R-4.4.1 R-foundation https://cran.r-project.org// Python 3.11 python.org https://www.python.org/downloads/release/ python-3110/ Cellpose 2.0 Stringer et al.8 https://github.com/MouseLand/cellpose/ STalign 1.0 Clifton et al.11 https://github.com/JEFworks-Lab/STalign FIJI/ImageJ 1.54f Schindelin et al.29 https://imagej.net/software/fiji/ SpatialData 0.2.3 Marconato et al.30 https://github.com/scverse/spatialdata Napari 0.5.4 Sofroniew et al.31 https://napari.org/stable/ Xenium Explorer 3.0 10× Genomics https://www.10xgenomics.com/support/software/ xenium-explorer/latest Xenium Ranger 3.0.1 10× Genomics https://www.10xgenomics.com/support/software/ xenium-ranger/latest sf 1.0–16 Pebesma and Bivand32 https://CRAN.R-project.org/package=sf sfheaders 0.4.4 Cooley33 https://CRAN.R-project.org/package=sfheaders lwgeom 0.2–14 Pebesma and Bivand32 https://postgis.net SingleR 2.6.0 Aran et al.34 https://bioconductor.org/packages/SingleR Seurat 5.1.0 Stuart et al.35 https://CRAN.R-project.org/package=Seurat (Continued on next page) e1 Cell Reports Methods 6, 101306, February 23, 2026 Article ll OPEN ACCESS ..
Saline:Article Title: Humidity shapes reproductive development in Arabidopsis thaliana by modulating epidermal identity and cell wall dynamics.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Technovit 7100 combipack Heraeus Kulzer Cat# 64709003 Nitroblue tetrazolium Sigma-Aldrich Cat#N6639 3,3-diaminobenzidine Sigma-Aldrich Cat#D5637 Toluidine blue O ThermoFisher Scientific Cat#BP10710 Piperonylic acid Sigma-Aldrich Cat#P49805 Coniferyl alcohol Sigma-Aldrich Cat#223735 Sinapyl alcohol Sigma-Aldrich Cat#404586 Phloroglucinol Sigma-Aldrich Cat#P3502 Epigallocatechin gallate Sigma-Aldrich Cat#44901 Pectinase from Aspergillus aculeatus Sigma-Aldrich Cat#P2611 Aminoethoxyvinylglycine Cayman Chemical Cat#15546 6-benzylaminopurine KisanBio Cat#B5812 β-estradiol Sigma-Aldrich Cat#43632 Paraformaldehyde Sigma-Aldrich Cat#A11313 Phosphate buffered saline Bio-Rad Cat1610780 ClearSee Ursache et al. 60 N/A Calcofluor whte Sigma-Aldrich Cat#18909 Basic fuchsin Sigma-Aldrich Cat#B0904 Propidium iodide Sigma-Aldrich Cat#537059 Fluorescein diacetate Sigma-Aldrich Cat#F7378 Tween 20 Sigma-Aldrich Cat#P1379 Lip balm Nivea Cat#88070 4-Head Industrial Commercial Electric Ultrasonic Atomizer Mist Maker Humidifier Fogger Foshan Nanhai Keri Electronic Co., Ltd. KERI M0403 Digital thermo-hygrometer SATO SK-110TRHII TYPE 4 Isopropanol Sigma-Aldrich Cat#278475 Hexane Sigma-Aldrich Cat#52750 Chloroform Sigma-Aldrich Cat#319988 Heptadecanoate Sigma-Aldrich Cat#286087 ω-pentadecalactone Sigma-Aldrich Cat#W284009 N,O-bis(trimethylsilyl) trifluoroacetamide Sigma-Aldrich Cat#155195 Pyridine Sigma-Aldrich Cat#360570 Heptane Sigma-Aldrich Cat#34873 Toluene solution Sigma-Aldrich Cat#CRM48620 Aniline Blue diammonium salt Sigma-Aldrich Cat#415049 amfiRivert cDNA Synthesis Mix GenDEPOT Cat#R5600-050 SYBR Green qPCR Master Mix ThermoFisher Scientific Cat#A25742 Critical commercial assays RNeasy Plant Mini Kit QIAGEN Cat#74094 TruSeq Stranded mRNA Library Prep kit Illumina Cat#20020611 Illumina HiSeq 2500 platform Illumina Cat#SY-401-2501 (Continued on next page) 18 Cell Reports 45, 116707, January 27, 2026 ..
Article Title: Ketone body supplementation into a standard high-carbohydrate diet reduces fecundity, delays larval development, and may induce autophagy in Drosophila melanogaster.
Article Snippet: Fasting (typically intermittent; IF), caloric restriction (CR), and the increasingly-popular high-fat, low-carbohydrate ketogenic diet (KD) count among many starvation-mimicking dietary regimens known to exert diverse effects on cellular and whole-organism behavioral, developmental, and physiological parameters.. These effects include lowering neuronal excitability, inducing the cellular-component-recycling process of autophagy, altering reproductive outcomes (especially in pathological cases), and extending lifespan.. These challenging diet regimens can produce elevated levels of circulating ketone bodies (KBs), which themselves are known to exert numerous potentially-beneficial genetic and signaling effects.
Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Monoclonal EZH1 antibody, 1:1000 dilution Cell Signaling Technology Cat# 42088; RRID: AB_2799212 Rabbit Monoclonal KMT6/EZH2 antibody, 1:1000 dilution Abcam Cat# ab191250; RRID: AB_2933983 Rabbit Monoclonal H3K27me3 antibody, 1:1000 dilution ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749. .. Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026
Article Title: An integrative spatial multi-omic workflow for unified analysis of tumor tissue.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Alexa Fluor® 647 phalloidin Thermo Fisher Scientific A22287 GFAP Thermo Fisher Scientific Cat# PA1-10004; RRID: AB_1074620 DAPI Thermo Fisher Scientific Cat# D1306; RRID: AB_2629482 Alexa Fluor 488 Plus Thermo Fisher Scientific Cat# A11039; RRID: AB_2534096 Nuclease-free Water (not DEPC treated) Thermo Fisher Scientific AM9937 TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0 (nuclease-free) Fisher Scientific BP24731 PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free Thermo Fisher Scientific AM9624 10% Tween 20 Bio-Rad 1662404 Ethyl alcohol, Pure (200 Proof, anhydrous) Millipore Sigma E7023-500ML Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 Methanol Methanol, for HPLC Millipore Sigma 34860 Critical commercial assays Xenium Slides & Sample Prep Reagents 10× Genomics PN-1000460 Xenium Decoding Consumables 10× Genomics PN-1000487 Xenium Instrument Accessory Kit, Module A 10× Genomics PN-1000530 Xenium Stand Alone Gene Expression Panel 10× Genomics PN-1000563 ZQ94XW Deposited data Analyzed ST data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Analyzed SM data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Processed tile scans This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Software and algorithms R-4.4.1 R-foundation https://cran.r-project.org// Python 3.11 python.org https://www.python.org/downloads/release/ python-3110/ Cellpose 2.0 Stringer et al.8 https://github.com/MouseLand/cellpose/ STalign 1.0 Clifton et al.11 https://github.com/JEFworks-Lab/STalign FIJI/ImageJ 1.54f Schindelin et al.29 https://imagej.net/software/fiji/ SpatialData 0.2.3 Marconato et al.30 https://github.com/scverse/spatialdata Napari 0.5.4 Sofroniew et al.31 https://napari.org/stable/ Xenium Explorer 3.0 10× Genomics https://www.10xgenomics.com/support/software/ xenium-explorer/latest Xenium Ranger 3.0.1 10× Genomics https://www.10xgenomics.com/support/software/ xenium-ranger/latest sf 1.0–16 Pebesma and Bivand32 https://CRAN.R-project.org/package=sf sfheaders 0.4.4 Cooley33 https://CRAN.R-project.org/package=sfheaders lwgeom 0.2–14 Pebesma and Bivand32 https://postgis.net SingleR 2.6.0 Aran et al.34 https://bioconductor.org/packages/SingleR Seurat 5.1.0 Stuart et al.35 https://CRAN.R-project.org/package=Seurat (Continued on next page) e1 Cell Reports Methods 6, 101306, February 23, 2026 Article ll OPEN ACCESS ..
cDNA Synthesis:Article Title: Humidity shapes reproductive development in Arabidopsis thaliana by modulating epidermal identity and cell wall dynamics.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Technovit 7100 combipack Heraeus Kulzer Cat# 64709003 Nitroblue tetrazolium Sigma-Aldrich Cat#N6639 3,3-diaminobenzidine Sigma-Aldrich Cat#D5637 Toluidine blue O ThermoFisher Scientific Cat#BP10710 Piperonylic acid Sigma-Aldrich Cat#P49805 Coniferyl alcohol Sigma-Aldrich Cat#223735 Sinapyl alcohol Sigma-Aldrich Cat#404586 Phloroglucinol Sigma-Aldrich Cat#P3502 Epigallocatechin gallate Sigma-Aldrich Cat#44901 Pectinase from Aspergillus aculeatus Sigma-Aldrich Cat#P2611 Aminoethoxyvinylglycine Cayman Chemical Cat#15546 6-benzylaminopurine KisanBio Cat#B5812 β-estradiol Sigma-Aldrich Cat#43632 Paraformaldehyde Sigma-Aldrich Cat#A11313 Phosphate buffered saline Bio-Rad Cat1610780 ClearSee Ursache et al. 60 N/A Calcofluor whte Sigma-Aldrich Cat#18909 Basic fuchsin Sigma-Aldrich Cat#B0904 Propidium iodide Sigma-Aldrich Cat#537059 Fluorescein diacetate Sigma-Aldrich Cat#F7378 Tween 20 Sigma-Aldrich Cat#P1379 Lip balm Nivea Cat#88070 4-Head Industrial Commercial Electric Ultrasonic Atomizer Mist Maker Humidifier Fogger Foshan Nanhai Keri Electronic Co., Ltd. KERI M0403 Digital thermo-hygrometer SATO SK-110TRHII TYPE 4 Isopropanol Sigma-Aldrich Cat#278475 Hexane Sigma-Aldrich Cat#52750 Chloroform Sigma-Aldrich Cat#319988 Heptadecanoate Sigma-Aldrich Cat#286087 ω-pentadecalactone Sigma-Aldrich Cat#W284009 N,O-bis(trimethylsilyl) trifluoroacetamide Sigma-Aldrich Cat#155195 Pyridine Sigma-Aldrich Cat#360570 Heptane Sigma-Aldrich Cat#34873 Toluene solution Sigma-Aldrich Cat#CRM48620 Aniline Blue diammonium salt Sigma-Aldrich Cat#415049 amfiRivert cDNA Synthesis Mix GenDEPOT Cat#R5600-050 SYBR Green qPCR Master Mix ThermoFisher Scientific Cat#A25742 Critical commercial assays RNeasy Plant Mini Kit QIAGEN Cat#74094 TruSeq Stranded mRNA Library Prep kit Illumina Cat#20020611 Illumina HiSeq 2500 platform Illumina Cat#SY-401-2501 (Continued on next page) 18 Cell Reports 45, 116707, January 27, 2026 ..
SYBR Green Assay:Article Title: Humidity shapes reproductive development in Arabidopsis thaliana by modulating epidermal identity and cell wall dynamics.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Technovit 7100 combipack Heraeus Kulzer Cat# 64709003 Nitroblue tetrazolium Sigma-Aldrich Cat#N6639 3,3-diaminobenzidine Sigma-Aldrich Cat#D5637 Toluidine blue O ThermoFisher Scientific Cat#BP10710 Piperonylic acid Sigma-Aldrich Cat#P49805 Coniferyl alcohol Sigma-Aldrich Cat#223735 Sinapyl alcohol Sigma-Aldrich Cat#404586 Phloroglucinol Sigma-Aldrich Cat#P3502 Epigallocatechin gallate Sigma-Aldrich Cat#44901 Pectinase from Aspergillus aculeatus Sigma-Aldrich Cat#P2611 Aminoethoxyvinylglycine Cayman Chemical Cat#15546 6-benzylaminopurine KisanBio Cat#B5812 β-estradiol Sigma-Aldrich Cat#43632 Paraformaldehyde Sigma-Aldrich Cat#A11313 Phosphate buffered saline Bio-Rad Cat1610780 ClearSee Ursache et al. 60 N/A Calcofluor whte Sigma-Aldrich Cat#18909 Basic fuchsin Sigma-Aldrich Cat#B0904 Propidium iodide Sigma-Aldrich Cat#537059 Fluorescein diacetate Sigma-Aldrich Cat#F7378 Tween 20 Sigma-Aldrich Cat#P1379 Lip balm Nivea Cat#88070 4-Head Industrial Commercial Electric Ultrasonic Atomizer Mist Maker Humidifier Fogger Foshan Nanhai Keri Electronic Co., Ltd. KERI M0403 Digital thermo-hygrometer SATO SK-110TRHII TYPE 4 Isopropanol Sigma-Aldrich Cat#278475 Hexane Sigma-Aldrich Cat#52750 Chloroform Sigma-Aldrich Cat#319988 Heptadecanoate Sigma-Aldrich Cat#286087 ω-pentadecalactone Sigma-Aldrich Cat#W284009 N,O-bis(trimethylsilyl) trifluoroacetamide Sigma-Aldrich Cat#155195 Pyridine Sigma-Aldrich Cat#360570 Heptane Sigma-Aldrich Cat#34873 Toluene solution Sigma-Aldrich Cat#CRM48620 Aniline Blue diammonium salt Sigma-Aldrich Cat#415049 amfiRivert cDNA Synthesis Mix GenDEPOT Cat#R5600-050 SYBR Green qPCR Master Mix ThermoFisher Scientific Cat#A25742 Critical commercial assays RNeasy Plant Mini Kit QIAGEN Cat#74094 TruSeq Stranded mRNA Library Prep kit Illumina Cat#20020611 Illumina HiSeq 2500 platform Illumina Cat#SY-401-2501 (Continued on next page) 18 Cell Reports 45, 116707, January 27, 2026 ..
Real-time Polymerase Chain Reaction:Article Title: Humidity shapes reproductive development in Arabidopsis thaliana by modulating epidermal identity and cell wall dynamics.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Technovit 7100 combipack Heraeus Kulzer Cat# 64709003 Nitroblue tetrazolium Sigma-Aldrich Cat#N6639 3,3-diaminobenzidine Sigma-Aldrich Cat#D5637 Toluidine blue O ThermoFisher Scientific Cat#BP10710 Piperonylic acid Sigma-Aldrich Cat#P49805 Coniferyl alcohol Sigma-Aldrich Cat#223735 Sinapyl alcohol Sigma-Aldrich Cat#404586 Phloroglucinol Sigma-Aldrich Cat#P3502 Epigallocatechin gallate Sigma-Aldrich Cat#44901 Pectinase from Aspergillus aculeatus Sigma-Aldrich Cat#P2611 Aminoethoxyvinylglycine Cayman Chemical Cat#15546 6-benzylaminopurine KisanBio Cat#B5812 β-estradiol Sigma-Aldrich Cat#43632 Paraformaldehyde Sigma-Aldrich Cat#A11313 Phosphate buffered saline Bio-Rad Cat1610780 ClearSee Ursache et al. 60 N/A Calcofluor whte Sigma-Aldrich Cat#18909 Basic fuchsin Sigma-Aldrich Cat#B0904 Propidium iodide Sigma-Aldrich Cat#537059 Fluorescein diacetate Sigma-Aldrich Cat#F7378 Tween 20 Sigma-Aldrich Cat#P1379 Lip balm Nivea Cat#88070 4-Head Industrial Commercial Electric Ultrasonic Atomizer Mist Maker Humidifier Fogger Foshan Nanhai Keri Electronic Co., Ltd. KERI M0403 Digital thermo-hygrometer SATO SK-110TRHII TYPE 4 Isopropanol Sigma-Aldrich Cat#278475 Hexane Sigma-Aldrich Cat#52750 Chloroform Sigma-Aldrich Cat#319988 Heptadecanoate Sigma-Aldrich Cat#286087 ω-pentadecalactone Sigma-Aldrich Cat#W284009 N,O-bis(trimethylsilyl) trifluoroacetamide Sigma-Aldrich Cat#155195 Pyridine Sigma-Aldrich Cat#360570 Heptane Sigma-Aldrich Cat#34873 Toluene solution Sigma-Aldrich Cat#CRM48620 Aniline Blue diammonium salt Sigma-Aldrich Cat#415049 amfiRivert cDNA Synthesis Mix GenDEPOT Cat#R5600-050 SYBR Green qPCR Master Mix ThermoFisher Scientific Cat#A25742 Critical commercial assays RNeasy Plant Mini Kit QIAGEN Cat#74094 TruSeq Stranded mRNA Library Prep kit Illumina Cat#20020611 Illumina HiSeq 2500 platform Illumina Cat#SY-401-2501 (Continued on next page) 18 Cell Reports 45, 116707, January 27, 2026 ..
Cell-Signaling:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Monoclonal EZH1 antibody, 1:1000 dilution Cell Signaling Technology Cat# 42088; RRID: AB_2799212 Rabbit Monoclonal KMT6/EZH2 antibody, 1:1000 dilution Abcam Cat# ab191250; RRID: AB_2933983 Rabbit Monoclonal H3K27me3 antibody, 1:1000 dilution ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749. .. Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026
Protease Inhibitor:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Monoclonal EZH1 antibody, 1:1000 dilution Cell Signaling Technology Cat# 42088; RRID: AB_2799212 Rabbit Monoclonal KMT6/EZH2 antibody, 1:1000 dilution Abcam Cat# ab191250; RRID: AB_2933983 Rabbit Monoclonal H3K27me3 antibody, 1:1000 dilution ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749. .. Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026
SDS Page:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Monoclonal EZH1 antibody, 1:1000 dilution Cell Signaling Technology Cat# 42088; RRID: AB_2799212 Rabbit Monoclonal KMT6/EZH2 antibody, 1:1000 dilution Abcam Cat# ab191250; RRID: AB_2933983 Rabbit Monoclonal H3K27me3 antibody, 1:1000 dilution ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749. .. Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026
Membrane:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Monoclonal EZH1 antibody, 1:1000 dilution Cell Signaling Technology Cat# 42088; RRID: AB_2799212 Rabbit Monoclonal KMT6/EZH2 antibody, 1:1000 dilution Abcam Cat# ab191250; RRID: AB_2933983 Rabbit Monoclonal H3K27me3 antibody, 1:1000 dilution ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749. .. Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026
Blocking Assay:Article Title: Dual impact of EZH1/2-targeted therapy in HTLV-1-associated inflammation: Direct and indirect mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Monoclonal EZH1 antibody, 1:1000 dilution Cell Signaling Technology Cat# 42088; RRID: AB_2799212 Rabbit Monoclonal KMT6/EZH2 antibody, 1:1000 dilution Abcam Cat# ab191250; RRID: AB_2933983 Rabbit Monoclonal H3K27me3 antibody, 1:1000 dilution ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749. .. Rabbit Polyclonal Histone H3 antibody, 1:2000 dilution Abcam Cat# ab1791; RRID: AB_302613 Mouse Monoclonal GAPDH antibody, 1:2000 dilution MBL Cat# M171-3; RRID: AB_10597731 Secondary antibody: Sheep anti-mouse IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA931; RRID: AB_772210 Secondary antibody: Donkey anti-rabbit IgG HRP-linked secondary antibody, 1:5000 dilution GE Healthcare (UK) Cat# NA934; RRID: AB_772206 Rabbit monoclonal NF-κB p65 (D14E12) antibody, 1:500 dilution Cell Signaling Technology Cat# 8242; RRID: AB_10859369 Goat anti-rabbit IgG H&L Alexa Fluor 488 secondary antibody, 1:2000 dilution Abcam Cat# ab150085 Chemicals, peptides, and recombinant proteins DMEM/F-12 Thermo Fisher Scientific (Gibco) Cat# 11320033 Fetal Bovine Serum (Heat-inactivated) Biowest Cat# S1810 Penicillin–Streptomycin (10,000 U/mL) Thermo Fisher Scientific Cat# 15140-122 Trabecular Meshwork Cell Medium ScienCell Research Laboratories Cat# 6591 Fetal Bovine Serum (for TM medium) GE Healthcare Japan Cat# SH30070.03 Fibroblast Growth Supplement ScienCell Research Laboratories Cat# 2352 RPMI-1640 Wako Pure Chemical Corporation Cat# 187-02705 EZH1/2 dual inhibitor (Valemetostat/DS-3201) Selleck Chemicals, Yokohama, Japan Cat# S8926 DMSO Wako Cat# 041-29351 Phosphate-Buffered Saline (PBS) Thermo Fisher Scientific Cat# 10010023 Paraformaldehyde, 4% Leagene Biotechnology Cat# DF0135 TritonTM X-100 Thermo Fisher Scientific Cat# 28314 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9418 TNF-α R&D Systems Cat# 210-TA-005 DAPI-Fluoromount-G Thermo Fisher Scientific Cat# 00-4959-52 Tween 20 Thermo Fisher Scientific Cat# BP337-500 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 87786 4–15% SDS-PAGE gel Bio-Rad Cat# 4561084 PVDF membrane Millipore Cat# IPVH00010 Bullet Blocking One Nacalai Tesque Cat# 13779-01 ECL solution Bio-Rad Cat# 1705060 RNase-free water Thermo Fisher Scientific Cat# AM9930 TBST Takara Cat# T972 10× Tris/Glycine/SDS Bio-Rad Cat# 1610732 10× Tris/Glycine Bio-Rad Cat# 1610734 10× PBS Bio-Rad Cat# 1610780 Ethanol 99.5% Wako Cat# 056-00485 (Continued on next page) iScience 29, 117775, November 20, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ponceau S Staining Solution Thermo Fisher Scientific Cat# A40000279 2-Mercaptoethanol Wako Cat# 13575-45 Laemmli buffer Bio-Rad Cat# 1610737 Normal Goat Serum Thermo Fisher Scientific Cat# 31872 TE buffer (PH = 8.0) FUJIFILM Cat# 314-90021 Critical commercial assays Annexin V-FITC/PI (propidium iodide) staining kit Abcam Cat# ab14085 CBA Human Inflammatory Cytokine kit BD, Biosciences Cat# 551811; RRID: AB_2868941 CBA Human Chemokine kit BD, Biosciences Cat# 552990; RRID: AB_2868970 Human IL-8 ELISA Kit Thermo Fisher Scientific Cat# KAC1301 Human IL-6 ELISA Kit Thermo Fisher Scientific Cat# EH2IL6 EZ1 Virus Mini Kit v2.0 QIAGEN Cat# 955134 CCK-8 assay AbMole Bioscience Cat# M4839 NF-κB InstantOne ELISA Kit eBioscience Cat# 85-86083-11 Protein concentration detection: BCA assay kit Thermo Fisher Scientific Cat# 23227 RNeasy Kits for RNA Purification Qiagen Cat# 74104 SuperScriptTM III PlatinumTM SYBRTM Green One-Step qRT-PCR Kit Thermo Fisher Scientific Cat# 11736051 Experimental models: Cell lines Human cell line: ARPE-19 ATCC Cat# CRL-2302; RRID: CVCL_0145 Primary human cells: hTMCs ScienCell Research Laboratories Cat# 6590 Human cell line: MT-2 JCRB Cell Bank (Osaka, Japan) JCRB1210; RRID: CVCL_2631 Human cell line: TL-Om1 The Institute of Medical Science, The University of Tokyo RRID: CVCL_B473 Human cell line: Jurkat ATCC Cat# TIB-152; RRID: CVCL_0367 Oligonucleotides RT-qPCR primers: EZH1-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAATTCAAGCTGGCGAAGAG-3′ RT-qPCR primers: EZH1-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CAAGACAGTGCCGCTACCA-3′ RT-qPCR primers: EZH2-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GCCAAGAGAGCCATCCAGAC-3′ RT-qPCR primers: EZH2-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCGACATACTTCAGGGCATCA-3′ RT-qPCR primers: Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- TCAACCCTCACCACTCCAGGC-3′ RT-qPCR primers: Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGGGCTCATGGTCATTGTCAT-3′ RT-qPCR primers: HBZ-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-AAGAGCATCGACGACATCGG-3′ RT-qPCR primers: HBZ-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′- GTGGGACAGGAGCTTGAAGT-3′ Proviral load primers: HTLV-1 Tax-F This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-CCCACTTCCCAGGGTTTGGA-3′ Proviral load primers: HTLV-1 Tax-R This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-GGCCAGTAGGGCGTGA-3′ Proviral load primers: probe This study; synthesized by NIHON TECHNO SERVICE CO., LTD 5′-FAM-CCAGTCTACGTGTTTG GAGACTGTGTACA-TAMRA-3′ (Continued on next page) 18 iScience 29, 117775, November 20, 2026
Immunofluorescence:Article Title: Connexin hemichannel blockade by abEC1.1 disrupts glioblastoma progression, suppresses invasiveness, and reduces hyperexcitability in preclinical models.
Article Snippet: .. Immunofluorescence staining of mouse brain sections Mouse brains were dissected, and post-fixed overnight in 4% paraformaldehyde (PFA, cat. n. P6148, Merck) in 1 × PBS (cat. n. 161–0780, Bio-Rad, CA, USA) at 4 °C. .. Upon embedding in 2% agarose (cat. n. A0575, Merck), the entire tissues were serially sectioned (50 μm coronal sections) using a Vibratome (VT1000 S, Leica Biosystems, IL, USA).
Staining:Article Title: Connexin hemichannel blockade by abEC1.1 disrupts glioblastoma progression, suppresses invasiveness, and reduces hyperexcitability in preclinical models.
Article Snippet: .. Immunofluorescence staining of mouse brain sections Mouse brains were dissected, and post-fixed overnight in 4% paraformaldehyde (PFA, cat. n. P6148, Merck) in 1 × PBS (cat. n. 161–0780, Bio-Rad, CA, USA) at 4 °C. .. Upon embedding in 2% agarose (cat. n. A0575, Merck), the entire tissues were serially sectioned (50 μm coronal sections) using a Vibratome (VT1000 S, Leica Biosystems, IL, USA).
Electron Microscopy:Article Title: An integrative spatial multi-omic workflow for unified analysis of tumor tissue.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Alexa Fluor® 647 phalloidin Thermo Fisher Scientific A22287 GFAP Thermo Fisher Scientific Cat# PA1-10004; RRID: AB_1074620 DAPI Thermo Fisher Scientific Cat# D1306; RRID: AB_2629482 Alexa Fluor 488 Plus Thermo Fisher Scientific Cat# A11039; RRID: AB_2534096 Nuclease-free Water (not DEPC treated) Thermo Fisher Scientific AM9937 TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0 (nuclease-free) Fisher Scientific BP24731 PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free Thermo Fisher Scientific AM9624 10% Tween 20 Bio-Rad 1662404 Ethyl alcohol, Pure (200 Proof, anhydrous) Millipore Sigma E7023-500ML Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 Methanol Methanol, for HPLC Millipore Sigma 34860 Critical commercial assays Xenium Slides & Sample Prep Reagents 10× Genomics PN-1000460 Xenium Decoding Consumables 10× Genomics PN-1000487 Xenium Instrument Accessory Kit, Module A 10× Genomics PN-1000530 Xenium Stand Alone Gene Expression Panel 10× Genomics PN-1000563 ZQ94XW Deposited data Analyzed ST data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Analyzed SM data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Processed tile scans This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Software and algorithms R-4.4.1 R-foundation https://cran.r-project.org// Python 3.11 python.org https://www.python.org/downloads/release/ python-3110/ Cellpose 2.0 Stringer et al.8 https://github.com/MouseLand/cellpose/ STalign 1.0 Clifton et al.11 https://github.com/JEFworks-Lab/STalign FIJI/ImageJ 1.54f Schindelin et al.29 https://imagej.net/software/fiji/ SpatialData 0.2.3 Marconato et al.30 https://github.com/scverse/spatialdata Napari 0.5.4 Sofroniew et al.31 https://napari.org/stable/ Xenium Explorer 3.0 10× Genomics https://www.10xgenomics.com/support/software/ xenium-explorer/latest Xenium Ranger 3.0.1 10× Genomics https://www.10xgenomics.com/support/software/ xenium-ranger/latest sf 1.0–16 Pebesma and Bivand32 https://CRAN.R-project.org/package=sf sfheaders 0.4.4 Cooley33 https://CRAN.R-project.org/package=sfheaders lwgeom 0.2–14 Pebesma and Bivand32 https://postgis.net SingleR 2.6.0 Aran et al.34 https://bioconductor.org/packages/SingleR Seurat 5.1.0 Stuart et al.35 https://CRAN.R-project.org/package=Seurat (Continued on next page) e1 Cell Reports Methods 6, 101306, February 23, 2026 Article ll OPEN ACCESS ..
High Performance Liquid Chromatography:Article Title: An integrative spatial multi-omic workflow for unified analysis of tumor tissue.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Alexa Fluor® 647 phalloidin Thermo Fisher Scientific A22287 GFAP Thermo Fisher Scientific Cat# PA1-10004; RRID: AB_1074620 DAPI Thermo Fisher Scientific Cat# D1306; RRID: AB_2629482 Alexa Fluor 488 Plus Thermo Fisher Scientific Cat# A11039; RRID: AB_2534096 Nuclease-free Water (not DEPC treated) Thermo Fisher Scientific AM9937 TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0 (nuclease-free) Fisher Scientific BP24731 PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free Thermo Fisher Scientific AM9624 10% Tween 20 Bio-Rad 1662404 Ethyl alcohol, Pure (200 Proof, anhydrous) Millipore Sigma E7023-500ML Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 Methanol Methanol, for HPLC Millipore Sigma 34860 Critical commercial assays Xenium Slides & Sample Prep Reagents 10× Genomics PN-1000460 Xenium Decoding Consumables 10× Genomics PN-1000487 Xenium Instrument Accessory Kit, Module A 10× Genomics PN-1000530 Xenium Stand Alone Gene Expression Panel 10× Genomics PN-1000563 ZQ94XW Deposited data Analyzed ST data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Analyzed SM data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Processed tile scans This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Software and algorithms R-4.4.1 R-foundation https://cran.r-project.org// Python 3.11 python.org https://www.python.org/downloads/release/ python-3110/ Cellpose 2.0 Stringer et al.8 https://github.com/MouseLand/cellpose/ STalign 1.0 Clifton et al.11 https://github.com/JEFworks-Lab/STalign FIJI/ImageJ 1.54f Schindelin et al.29 https://imagej.net/software/fiji/ SpatialData 0.2.3 Marconato et al.30 https://github.com/scverse/spatialdata Napari 0.5.4 Sofroniew et al.31 https://napari.org/stable/ Xenium Explorer 3.0 10× Genomics https://www.10xgenomics.com/support/software/ xenium-explorer/latest Xenium Ranger 3.0.1 10× Genomics https://www.10xgenomics.com/support/software/ xenium-ranger/latest sf 1.0–16 Pebesma and Bivand32 https://CRAN.R-project.org/package=sf sfheaders 0.4.4 Cooley33 https://CRAN.R-project.org/package=sfheaders lwgeom 0.2–14 Pebesma and Bivand32 https://postgis.net SingleR 2.6.0 Aran et al.34 https://bioconductor.org/packages/SingleR Seurat 5.1.0 Stuart et al.35 https://CRAN.R-project.org/package=Seurat (Continued on next page) e1 Cell Reports Methods 6, 101306, February 23, 2026 Article ll OPEN ACCESS ..
Sample Prep:Article Title: An integrative spatial multi-omic workflow for unified analysis of tumor tissue.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Alexa Fluor® 647 phalloidin Thermo Fisher Scientific A22287 GFAP Thermo Fisher Scientific Cat# PA1-10004; RRID: AB_1074620 DAPI Thermo Fisher Scientific Cat# D1306; RRID: AB_2629482 Alexa Fluor 488 Plus Thermo Fisher Scientific Cat# A11039; RRID: AB_2534096 Nuclease-free Water (not DEPC treated) Thermo Fisher Scientific AM9937 TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0 (nuclease-free) Fisher Scientific BP24731 PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free Thermo Fisher Scientific AM9624 10% Tween 20 Bio-Rad 1662404 Ethyl alcohol, Pure (200 Proof, anhydrous) Millipore Sigma E7023-500ML Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 Methanol Methanol, for HPLC Millipore Sigma 34860 Critical commercial assays Xenium Slides & Sample Prep Reagents 10× Genomics PN-1000460 Xenium Decoding Consumables 10× Genomics PN-1000487 Xenium Instrument Accessory Kit, Module A 10× Genomics PN-1000530 Xenium Stand Alone Gene Expression Panel 10× Genomics PN-1000563 ZQ94XW Deposited data Analyzed ST data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Analyzed SM data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Processed tile scans This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Software and algorithms R-4.4.1 R-foundation https://cran.r-project.org// Python 3.11 python.org https://www.python.org/downloads/release/ python-3110/ Cellpose 2.0 Stringer et al.8 https://github.com/MouseLand/cellpose/ STalign 1.0 Clifton et al.11 https://github.com/JEFworks-Lab/STalign FIJI/ImageJ 1.54f Schindelin et al.29 https://imagej.net/software/fiji/ SpatialData 0.2.3 Marconato et al.30 https://github.com/scverse/spatialdata Napari 0.5.4 Sofroniew et al.31 https://napari.org/stable/ Xenium Explorer 3.0 10× Genomics https://www.10xgenomics.com/support/software/ xenium-explorer/latest Xenium Ranger 3.0.1 10× Genomics https://www.10xgenomics.com/support/software/ xenium-ranger/latest sf 1.0–16 Pebesma and Bivand32 https://CRAN.R-project.org/package=sf sfheaders 0.4.4 Cooley33 https://CRAN.R-project.org/package=sfheaders lwgeom 0.2–14 Pebesma and Bivand32 https://postgis.net SingleR 2.6.0 Aran et al.34 https://bioconductor.org/packages/SingleR Seurat 5.1.0 Stuart et al.35 https://CRAN.R-project.org/package=Seurat (Continued on next page) e1 Cell Reports Methods 6, 101306, February 23, 2026 Article ll OPEN ACCESS ..
Gene Expression:Article Title: An integrative spatial multi-omic workflow for unified analysis of tumor tissue.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Alexa Fluor® 647 phalloidin Thermo Fisher Scientific A22287 GFAP Thermo Fisher Scientific Cat# PA1-10004; RRID: AB_1074620 DAPI Thermo Fisher Scientific Cat# D1306; RRID: AB_2629482 Alexa Fluor 488 Plus Thermo Fisher Scientific Cat# A11039; RRID: AB_2534096 Nuclease-free Water (not DEPC treated) Thermo Fisher Scientific AM9937 TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0 (nuclease-free) Fisher Scientific BP24731 PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free Thermo Fisher Scientific AM9624 10% Tween 20 Bio-Rad 1662404 Ethyl alcohol, Pure (200 Proof, anhydrous) Millipore Sigma E7023-500ML Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 Methanol Methanol, for HPLC Millipore Sigma 34860 Critical commercial assays Xenium Slides & Sample Prep Reagents 10× Genomics PN-1000460 Xenium Decoding Consumables 10× Genomics PN-1000487 Xenium Instrument Accessory Kit, Module A 10× Genomics PN-1000530 Xenium Stand Alone Gene Expression Panel 10× Genomics PN-1000563 ZQ94XW Deposited data Analyzed ST data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Analyzed SM data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Processed tile scans This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Software and algorithms R-4.4.1 R-foundation https://cran.r-project.org// Python 3.11 python.org https://www.python.org/downloads/release/ python-3110/ Cellpose 2.0 Stringer et al.8 https://github.com/MouseLand/cellpose/ STalign 1.0 Clifton et al.11 https://github.com/JEFworks-Lab/STalign FIJI/ImageJ 1.54f Schindelin et al.29 https://imagej.net/software/fiji/ SpatialData 0.2.3 Marconato et al.30 https://github.com/scverse/spatialdata Napari 0.5.4 Sofroniew et al.31 https://napari.org/stable/ Xenium Explorer 3.0 10× Genomics https://www.10xgenomics.com/support/software/ xenium-explorer/latest Xenium Ranger 3.0.1 10× Genomics https://www.10xgenomics.com/support/software/ xenium-ranger/latest sf 1.0–16 Pebesma and Bivand32 https://CRAN.R-project.org/package=sf sfheaders 0.4.4 Cooley33 https://CRAN.R-project.org/package=sfheaders lwgeom 0.2–14 Pebesma and Bivand32 https://postgis.net SingleR 2.6.0 Aran et al.34 https://bioconductor.org/packages/SingleR Seurat 5.1.0 Stuart et al.35 https://CRAN.R-project.org/package=Seurat (Continued on next page) e1 Cell Reports Methods 6, 101306, February 23, 2026 Article ll OPEN ACCESS ..
Software:Article Title: An integrative spatial multi-omic workflow for unified analysis of tumor tissue.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Alexa Fluor® 647 phalloidin Thermo Fisher Scientific A22287 GFAP Thermo Fisher Scientific Cat# PA1-10004; RRID: AB_1074620 DAPI Thermo Fisher Scientific Cat# D1306; RRID: AB_2629482 Alexa Fluor 488 Plus Thermo Fisher Scientific Cat# A11039; RRID: AB_2534096 Nuclease-free Water (not DEPC treated) Thermo Fisher Scientific AM9937 TE Buffer, TRIS-EDTA, 1X Solution, pH 8.0 (nuclease-free) Fisher Scientific BP24731 PBS - Phosphate Buffered Saline (10X) pH 7.4, RNase-free Thermo Fisher Scientific AM9624 10% Tween 20 Bio-Rad 1662404 Ethyl alcohol, Pure (200 Proof, anhydrous) Millipore Sigma E7023-500ML Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 Methanol Methanol, for HPLC Millipore Sigma 34860 Critical commercial assays Xenium Slides & Sample Prep Reagents 10× Genomics PN-1000460 Xenium Decoding Consumables 10× Genomics PN-1000487 Xenium Instrument Accessory Kit, Module A 10× Genomics PN-1000530 Xenium Stand Alone Gene Expression Panel 10× Genomics PN-1000563 ZQ94XW Deposited data Analyzed ST data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Analyzed SM data This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Processed tile scans This paper https://www.ebi.ac.uk/biostudies/bioimages/ studies/S-BIAD1426 Software and algorithms R-4.4.1 R-foundation https://cran.r-project.org// Python 3.11 python.org https://www.python.org/downloads/release/ python-3110/ Cellpose 2.0 Stringer et al.8 https://github.com/MouseLand/cellpose/ STalign 1.0 Clifton et al.11 https://github.com/JEFworks-Lab/STalign FIJI/ImageJ 1.54f Schindelin et al.29 https://imagej.net/software/fiji/ SpatialData 0.2.3 Marconato et al.30 https://github.com/scverse/spatialdata Napari 0.5.4 Sofroniew et al.31 https://napari.org/stable/ Xenium Explorer 3.0 10× Genomics https://www.10xgenomics.com/support/software/ xenium-explorer/latest Xenium Ranger 3.0.1 10× Genomics https://www.10xgenomics.com/support/software/ xenium-ranger/latest sf 1.0–16 Pebesma and Bivand32 https://CRAN.R-project.org/package=sf sfheaders 0.4.4 Cooley33 https://CRAN.R-project.org/package=sfheaders lwgeom 0.2–14 Pebesma and Bivand32 https://postgis.net SingleR 2.6.0 Aran et al.34 https://bioconductor.org/packages/SingleR Seurat 5.1.0 Stuart et al.35 https://CRAN.R-project.org/package=Seurat (Continued on next page) e1 Cell Reports Methods 6, 101306, February 23, 2026 Article ll OPEN ACCESS ..
|
Bio-Rad is a verified supplier 
Bio-Rad manufactures this product 