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pgreenlantern 1  (Addgene inc)


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    Structured Review

    Addgene inc pgreenlantern 1
    Pgreenlantern 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgreenlantern+vector/pRS316CUP+RNQ+CFP+(Plasmid+%231171)/pmc05420564-26-9-10
    Average 92 stars, based on 7 article reviews
    pgreenlantern 1 - by Bioz Stars, 2026-11
    92/100 stars

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    Construct:

    Article Title: PIN1 Modulates Huntingtin Levels and Aggregate Accumulation: An In vitro Model
    Article Snippet: Httex1Q60GFP in pcDNA3.0 (Invitrogen), encoding the first exon (1–85 aa) of human HTT with 60 glutamines in frame with GFP, was constructed by cloning PCR amplified HTT exon1 into EcoRI-XhoI sites of pcDNA3.0GFP vector. .. The GFP moiety was recovered by XhoI digestion from pGreenLantern-1 (Addgene). htt 1–171 Q21/Q60GFP, encoding the N-terminal 171 amino acids of human HTT, with 21 and 60 glutamines respectively, was constructed as previously described (Persichetti et al., ; by subcloning the NcoI-XhoI fragment of HTT cDNA into pcDNA3.0GFP vector). ..

    Article Title: Functional amyloid protein FXR1 is recruited into neuronal stress granules
    Article Snippet: The TIA-1 gene was amplified by PCR from the pET28a_TIA1 plasmid (Addgene, #106095) using the primers TIA1_For and TIA1_Rev presented in . .. The pRS316Cup-FXR1-CFP(URA3) and pRS425Cup-FXR1-YFP(LEU2) plasmids were constructed by the following scheme: the sequence encoding human FXR1, flanked by the Hind III and BamH I restriction sites, was amplified by PCR from the pFRT-TODestFLAGHAhFXR1 (Addgene, #48694) with FXR1_For and FXR1_Rev primers ( ). ..

    Article Title: Functional amyloid protein FXR1 is recruited into neuronal stress granules.
    Article Snippet: The TIA-1 gene was amplified by PCR from the pET28a_TIA1 plasmid (Addgene, #106095) using the primers TIA1_For and TIA1_Rev presented in Table 1. .. The pRS316Cup-FXR1-CFP(URA3) and pRS425Cup-FXR1-YFP(LEU2) plasmids were constructed by the following scheme: the sequence encoding human FXR1, flanked by the HindIII and BamHI restriction sites, was amplified by PCR from the pFRT-TODestFLAGHAhFXR1 (Addgene, #48694) with FXR1_For and FXR1_Rev primers (Table 1). ..

    Subcloning:

    Article Title: PIN1 Modulates Huntingtin Levels and Aggregate Accumulation: An In vitro Model
    Article Snippet: Httex1Q60GFP in pcDNA3.0 (Invitrogen), encoding the first exon (1–85 aa) of human HTT with 60 glutamines in frame with GFP, was constructed by cloning PCR amplified HTT exon1 into EcoRI-XhoI sites of pcDNA3.0GFP vector. .. The GFP moiety was recovered by XhoI digestion from pGreenLantern-1 (Addgene). htt 1–171 Q21/Q60GFP, encoding the N-terminal 171 amino acids of human HTT, with 21 and 60 glutamines respectively, was constructed as previously described (Persichetti et al., ; by subcloning the NcoI-XhoI fragment of HTT cDNA into pcDNA3.0GFP vector). ..

    Retroviral:

    Article Title: Modulators of telomere disease
    Article Snippet: Lentiviral vectors encoding EGFP and PARN were cloned into pLX301 (puromycin; Addgene #25895) and pLX304. .. Retroviral and lentiviral vectors pBABE-hTERT-puro (Addgene #1171), p-MIG-DKC1 and pHIV7/SF-U3-TER-500 and controls were described in e.g., Agarwal, Suneet, et al. “Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients.” Nature 464.7286 (2010): 292-296. .. Viral vector production and transduction.

    Sequencing:

    Article Title: Functional amyloid protein FXR1 is recruited into neuronal stress granules
    Article Snippet: The TIA-1 gene was amplified by PCR from the pET28a_TIA1 plasmid (Addgene, #106095) using the primers TIA1_For and TIA1_Rev presented in . .. The pRS316Cup-FXR1-CFP(URA3) and pRS425Cup-FXR1-YFP(LEU2) plasmids were constructed by the following scheme: the sequence encoding human FXR1, flanked by the Hind III and BamH I restriction sites, was amplified by PCR from the pFRT-TODestFLAGHAhFXR1 (Addgene, #48694) with FXR1_For and FXR1_Rev primers ( ). ..

    Article Title: Functional amyloid protein FXR1 is recruited into neuronal stress granules.
    Article Snippet: The TIA-1 gene was amplified by PCR from the pET28a_TIA1 plasmid (Addgene, #106095) using the primers TIA1_For and TIA1_Rev presented in Table 1. .. The pRS316Cup-FXR1-CFP(URA3) and pRS425Cup-FXR1-YFP(LEU2) plasmids were constructed by the following scheme: the sequence encoding human FXR1, flanked by the HindIII and BamHI restriction sites, was amplified by PCR from the pFRT-TODestFLAGHAhFXR1 (Addgene, #48694) with FXR1_For and FXR1_Rev primers (Table 1). ..

    Amplification:

    Article Title: Functional amyloid protein FXR1 is recruited into neuronal stress granules
    Article Snippet: The TIA-1 gene was amplified by PCR from the pET28a_TIA1 plasmid (Addgene, #106095) using the primers TIA1_For and TIA1_Rev presented in . .. The pRS316Cup-FXR1-CFP(URA3) and pRS425Cup-FXR1-YFP(LEU2) plasmids were constructed by the following scheme: the sequence encoding human FXR1, flanked by the Hind III and BamH I restriction sites, was amplified by PCR from the pFRT-TODestFLAGHAhFXR1 (Addgene, #48694) with FXR1_For and FXR1_Rev primers ( ). ..

    Article Title: Functional amyloid protein FXR1 is recruited into neuronal stress granules.
    Article Snippet: The TIA-1 gene was amplified by PCR from the pET28a_TIA1 plasmid (Addgene, #106095) using the primers TIA1_For and TIA1_Rev presented in Table 1. .. The pRS316Cup-FXR1-CFP(URA3) and pRS425Cup-FXR1-YFP(LEU2) plasmids were constructed by the following scheme: the sequence encoding human FXR1, flanked by the HindIII and BamHI restriction sites, was amplified by PCR from the pFRT-TODestFLAGHAhFXR1 (Addgene, #48694) with FXR1_For and FXR1_Rev primers (Table 1). ..

    Polymerase Chain Reaction:

    Article Title: Functional amyloid protein FXR1 is recruited into neuronal stress granules
    Article Snippet: The TIA-1 gene was amplified by PCR from the pET28a_TIA1 plasmid (Addgene, #106095) using the primers TIA1_For and TIA1_Rev presented in . .. The pRS316Cup-FXR1-CFP(URA3) and pRS425Cup-FXR1-YFP(LEU2) plasmids were constructed by the following scheme: the sequence encoding human FXR1, flanked by the Hind III and BamH I restriction sites, was amplified by PCR from the pFRT-TODestFLAGHAhFXR1 (Addgene, #48694) with FXR1_For and FXR1_Rev primers ( ). ..

    Article Title: Functional amyloid protein FXR1 is recruited into neuronal stress granules.
    Article Snippet: The TIA-1 gene was amplified by PCR from the pET28a_TIA1 plasmid (Addgene, #106095) using the primers TIA1_For and TIA1_Rev presented in Table 1. .. The pRS316Cup-FXR1-CFP(URA3) and pRS425Cup-FXR1-YFP(LEU2) plasmids were constructed by the following scheme: the sequence encoding human FXR1, flanked by the HindIII and BamHI restriction sites, was amplified by PCR from the pFRT-TODestFLAGHAhFXR1 (Addgene, #48694) with FXR1_For and FXR1_Rev primers (Table 1). ..

    Plasmid Preparation:

    Article Title: An aggregation-prone mutant of eIF3a forms reversible assemblies escaping spatial control in exponentially growing yeast cells.
    Article Snippet: Cells have elaborated a complex strategy to maintain protein homeostasis under physiological as well as stress conditions with the aim to ensure the smooth functioning of vital processes and producing healthy offspring.. Impairment of one of the most important processes in living cells, translation, might have serious consequences including various brain disorders in humans.. Here, we describe a variant of the translation initiation factor eIF3a, Rpg1-3, mutated in its PCI domain that displays an attenuated translation efficiency and formation of reversible assemblies at physiological growth conditions.



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