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pgl3 basic luciferase reporter vectors  (Genechem)

 
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    Genechem pgl3 basic luciferase reporter vectors
    Pgl3 Basic Luciferase Reporter Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3-basic+vector/abcb11+construct+gv238+luciferase+promoter+reporter/pmc12622415-72-22-26
    Average 86 stars, based on 1 article reviews
    pgl3 basic luciferase reporter vectors - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Luciferase:

    Article Title: The NR4A1-IRF1 axis suppresses dendritic cell maturation to promote an immunosuppressive microenvironment in osteosarcoma
    Article Snippet: .. Firefly luciferase reporter plasmids, driven by the wild-type (AAATGACA) or mutant IRF1 promoter sequence (ACGCCGTA), were commercially synthesized by GeneChem (Shanghai, China). ..

    Article Title: HIF-1α suppresses SNPH expression to facilitate liver metastasis of colorectal cancer through regulating mitochondrial dynamics and filopodia formation
    Article Snippet: Images were acquired on a TEM (HITACHI, Japan). .. A total of 2.5 × 10 4 CRC were co-transfected with GV238-MIR130A-HG-promoter (Genechem, China) and siNC or siHIF-1α (Genepharma, China) and cultured in 5% CO 2 for 12 h, followed by 1% O 2 for 24 h. To assess the luciferase activity of the cell lysates, a Dual-Luciferase Reporter System (Promega, United States) was employed. .. In a separate experiment, a total of 2.5 × 10 4 CRC were co-transfected with miR-130a-3p mimic and pmirGLO-SNPH-MUT or pmirGLO-SNPH-WT (Genepharma, China).

    Article Title: Hepatic CBP/p300 maintain bile acid homeostasis through histone acetylation-mediated KLF10-BSEP axis.
    Article Snippet: .. Cells were co-transfected 518 with the GV238-Abcb11-promoter luciferase reporter construct (Genechem, Shanghai, China), 519 the CV702-Klf10 expression plasmid (Genechem, Shanghai, China), and the internal control 520 plasmid pRL-TK (Promega, Madison, WI, USA) using Lipofectamine 2000 (Gibco, 521 11668019). .. After 24 hours, luciferase activity was measured using the Dual-Luciferase 522 Reporter Assay System (Promega, Beijing, China) and detected with a Spark multimode 523 microplate reader (Tecan Group Ltd.).

    Article Title: IRP1/ARID3A complex promotes pancreatic cancer chemoresistance by suppressing CYGB-related ferroptosis
    Article Snippet: .. After 24 h of culture, the cells were co-transfected with the designed firefly luciferase reporter plasmids, Renilla luciferase plasmids, and corresponding empty vectors (Genechem, China). .. Firefly and Renilla fluorescence signals were detected 48 h after transfection using a dual luciferase reporter assay kit (YEASEN, Shanghai, China) by a fluorescence microplate reader.

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Article Title: Necrotizing enterocolitis is exacerbated through SUCNR1/HIF-1α/BNIP3 axis-mediated succinate-induced intestinal epithelial cell apoptosis.
    Article Snippet: BACKGROUND: To elucidate the mechanism by which succinate exacerbates intestinal injury in necrotizing enterocolitis (NEC).. METHODS: Succinate levels were measured in newborns and mice with NEC.. Intestinal epithelial damage and apoptosis were evaluated in NEC models.

    Mutagenesis:

    Article Title: The NR4A1-IRF1 axis suppresses dendritic cell maturation to promote an immunosuppressive microenvironment in osteosarcoma
    Article Snippet: .. Firefly luciferase reporter plasmids, driven by the wild-type (AAATGACA) or mutant IRF1 promoter sequence (ACGCCGTA), were commercially synthesized by GeneChem (Shanghai, China). ..

    Sequencing:

    Article Title: The NR4A1-IRF1 axis suppresses dendritic cell maturation to promote an immunosuppressive microenvironment in osteosarcoma
    Article Snippet: .. Firefly luciferase reporter plasmids, driven by the wild-type (AAATGACA) or mutant IRF1 promoter sequence (ACGCCGTA), were commercially synthesized by GeneChem (Shanghai, China). ..

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Synthesized:

    Article Title: The NR4A1-IRF1 axis suppresses dendritic cell maturation to promote an immunosuppressive microenvironment in osteosarcoma
    Article Snippet: .. Firefly luciferase reporter plasmids, driven by the wild-type (AAATGACA) or mutant IRF1 promoter sequence (ACGCCGTA), were commercially synthesized by GeneChem (Shanghai, China). ..

    Reporter Assay:

    Article Title: HIF-1α suppresses SNPH expression to facilitate liver metastasis of colorectal cancer through regulating mitochondrial dynamics and filopodia formation.
    Article Snippet: Images were acquired on a TEM (HITACHI, Japan). .. Luciferase Reporter Assay AR TI CL E IN P RE SS A total of 2.5×104 CRC were co-transfected with GV238-MIR130A-HG-promoter (Genechem, China) and siNC or siHIF-1α (Genepharma, China) and cultured in 5% CO2 for 12 hours followed by 1% O2 for 24 hours. .. To assess the luciferase activity of the cell lysates, a Dual-Luciferase Reporter System (Promega, United States) was employed.

    Cell Culture:

    Article Title: HIF-1α suppresses SNPH expression to facilitate liver metastasis of colorectal cancer through regulating mitochondrial dynamics and filopodia formation.
    Article Snippet: Images were acquired on a TEM (HITACHI, Japan). .. Luciferase Reporter Assay AR TI CL E IN P RE SS A total of 2.5×104 CRC were co-transfected with GV238-MIR130A-HG-promoter (Genechem, China) and siNC or siHIF-1α (Genepharma, China) and cultured in 5% CO2 for 12 hours followed by 1% O2 for 24 hours. .. To assess the luciferase activity of the cell lysates, a Dual-Luciferase Reporter System (Promega, United States) was employed.

    Article Title: HIF-1α suppresses SNPH expression to facilitate liver metastasis of colorectal cancer through regulating mitochondrial dynamics and filopodia formation
    Article Snippet: Images were acquired on a TEM (HITACHI, Japan). .. A total of 2.5 × 10 4 CRC were co-transfected with GV238-MIR130A-HG-promoter (Genechem, China) and siNC or siHIF-1α (Genepharma, China) and cultured in 5% CO 2 for 12 h, followed by 1% O 2 for 24 h. To assess the luciferase activity of the cell lysates, a Dual-Luciferase Reporter System (Promega, United States) was employed. .. In a separate experiment, a total of 2.5 × 10 4 CRC were co-transfected with miR-130a-3p mimic and pmirGLO-SNPH-MUT or pmirGLO-SNPH-WT (Genepharma, China).

    Activity Assay:

    Article Title: HIF-1α suppresses SNPH expression to facilitate liver metastasis of colorectal cancer through regulating mitochondrial dynamics and filopodia formation
    Article Snippet: Images were acquired on a TEM (HITACHI, Japan). .. A total of 2.5 × 10 4 CRC were co-transfected with GV238-MIR130A-HG-promoter (Genechem, China) and siNC or siHIF-1α (Genepharma, China) and cultured in 5% CO 2 for 12 h, followed by 1% O 2 for 24 h. To assess the luciferase activity of the cell lysates, a Dual-Luciferase Reporter System (Promega, United States) was employed. .. In a separate experiment, a total of 2.5 × 10 4 CRC were co-transfected with miR-130a-3p mimic and pmirGLO-SNPH-MUT or pmirGLO-SNPH-WT (Genepharma, China).

    Over Expression:

    Article Title: Nicotinamide Mononucleotide Ameliorates Nano-Aluminum Oxide-Induced Cognitive Impairment and Ferroptosis via the GSH/GPX4 Axis.
    Article Snippet: Aluminum oxide nanoparticles (Nano-Al2O3) are widely used engineered nanomaterials, but their neurotoxic effects and underlying mechanisms remain incompletely understood.. Nicotinamide mononucleotide (NMN), a precursor to nicotinamide adenine dinucleotide (NAD), possesses antioxidant properties andneuroprotective potential.. In this study,we investigatedwhether NMN mitigates Nano-Al2O3-induced cognitive impairment and neuronal injury, with a focus on ferroptosis-related pathways.

    Plasmid Preparation:

    Article Title: Nicotinamide Mononucleotide Ameliorates Nano-Aluminum Oxide-Induced Cognitive Impairment and Ferroptosis via the GSH/GPX4 Axis.
    Article Snippet: Aluminum oxide nanoparticles (Nano-Al2O3) are widely used engineered nanomaterials, but their neurotoxic effects and underlying mechanisms remain incompletely understood.. Nicotinamide mononucleotide (NMN), a precursor to nicotinamide adenine dinucleotide (NAD), possesses antioxidant properties andneuroprotective potential.. In this study,we investigatedwhether NMN mitigates Nano-Al2O3-induced cognitive impairment and neuronal injury, with a focus on ferroptosis-related pathways.

    Article Title: Hepatic CBP/p300 maintain bile acid homeostasis through histone acetylation-mediated KLF10-BSEP axis.
    Article Snippet: .. Cells were co-transfected 518 with the GV238-Abcb11-promoter luciferase reporter construct (Genechem, Shanghai, China), 519 the CV702-Klf10 expression plasmid (Genechem, Shanghai, China), and the internal control 520 plasmid pRL-TK (Promega, Madison, WI, USA) using Lipofectamine 2000 (Gibco, 521 11668019). .. After 24 hours, luciferase activity was measured using the Dual-Luciferase 522 Reporter Assay System (Promega, Beijing, China) and detected with a Spark multimode 523 microplate reader (Tecan Group Ltd.).

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Construct:

    Article Title: Hepatic CBP/p300 maintain bile acid homeostasis through histone acetylation-mediated KLF10-BSEP axis.
    Article Snippet: .. Cells were co-transfected 518 with the GV238-Abcb11-promoter luciferase reporter construct (Genechem, Shanghai, China), 519 the CV702-Klf10 expression plasmid (Genechem, Shanghai, China), and the internal control 520 plasmid pRL-TK (Promega, Madison, WI, USA) using Lipofectamine 2000 (Gibco, 521 11668019). .. After 24 hours, luciferase activity was measured using the Dual-Luciferase 522 Reporter Assay System (Promega, Beijing, China) and detected with a Spark multimode 523 microplate reader (Tecan Group Ltd.).

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Expressing:

    Article Title: Hepatic CBP/p300 maintain bile acid homeostasis through histone acetylation-mediated KLF10-BSEP axis.
    Article Snippet: .. Cells were co-transfected 518 with the GV238-Abcb11-promoter luciferase reporter construct (Genechem, Shanghai, China), 519 the CV702-Klf10 expression plasmid (Genechem, Shanghai, China), and the internal control 520 plasmid pRL-TK (Promega, Madison, WI, USA) using Lipofectamine 2000 (Gibco, 521 11668019). .. After 24 hours, luciferase activity was measured using the Dual-Luciferase 522 Reporter Assay System (Promega, Beijing, China) and detected with a Spark multimode 523 microplate reader (Tecan Group Ltd.).

    Control:

    Article Title: Hepatic CBP/p300 maintain bile acid homeostasis through histone acetylation-mediated KLF10-BSEP axis.
    Article Snippet: .. Cells were co-transfected 518 with the GV238-Abcb11-promoter luciferase reporter construct (Genechem, Shanghai, China), 519 the CV702-Klf10 expression plasmid (Genechem, Shanghai, China), and the internal control 520 plasmid pRL-TK (Promega, Madison, WI, USA) using Lipofectamine 2000 (Gibco, 521 11668019). .. After 24 hours, luciferase activity was measured using the Dual-Luciferase 522 Reporter Assay System (Promega, Beijing, China) and detected with a Spark multimode 523 microplate reader (Tecan Group Ltd.).

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Cloning:

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Reporter Gene Assay:

    Article Title: Necrotizing enterocolitis is exacerbated through SUCNR1/HIF-1α/BNIP3 axis-mediated succinate-induced intestinal epithelial cell apoptosis.
    Article Snippet: BACKGROUND: To elucidate the mechanism by which succinate exacerbates intestinal injury in necrotizing enterocolitis (NEC).. METHODS: Succinate levels were measured in newborns and mice with NEC.. Intestinal epithelial damage and apoptosis were evaluated in NEC models.



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